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The map label for this gene is purU [H]

Identifier: 37523894

GI number: 37523894

Start: 4557889

End: 4558791

Strand: Reverse

Name: purU [H]

Synonym: gll4325

Alternate gene names: 37523894

Gene position: 4558791-4557889 (Counterclockwise)

Preceding gene: 37523895

Following gene: 37523892

Centisome position: 97.85

GC content: 62.13

Gene sequence:

>903_bases
ATGCTTGTGCTTTTGCCGCCGGATTGTCCGGCACCCGACACCGCGATGTCCCCTGCCACCCGCACCCTACTCATTTCCTG
CCCCGACCGCCGGGGAATCGTGGCCGCCGTCTCGCAGTGCGTGTTGGAGGCGGGGGGCAACATCTTGCGCTCCGACCAGC
ACACCACCGACCCGCTCGGGGGCATCTTCTTCATGCGCCTCGAATTTCTCTACGCGAACGCGCCTGCCGACGACGACGGC
TTCGCCCGCTGCTTCGCGCCCGTGGCCGAGCGCTTTGCGATGGACTGGCGGCTGGTGCGCACGGGCGAACCCAAGCGCAT
GGCGCTATTCGTCTCGCGGCTGGACCATTGCTTTGTCGATTTGCTGTGGCGCCGGCAGTCGGGAGAACTGCCTGTCAAGA
TCCCGCTGGTGGTGAGCAACCACCCCGATCTCGAACCGGTGGCCGCCCAGTACGGTCTGCCGTACCATTACCTCGCGATT
GACAAAACCAACCAGCCCGCCCGCGAAGCCCAGATGCTCAACTTGCTCGAAGGTGAGGTCGATTTCATCGTTCTGGCCCG
CTACATGCGCGTACTGAGCCCACAGTTTGTGGAACGTTACGCCGGGCGGATTATTAATATCCACCACAGCTTCCTGCCTG
CCTTCGTGGGCGCTTCGCCATACGAGCGAGCCTGCGAGCGGGGAGTGAAAGTGATCGGCGCCACCGCCCACTACGTCACC
GAAGAGTTGGACGCGGGGCCGATTATCGAGCAGGACGTGGTGCGGGTCAACCATCGAGACCAGGTAGCCGATCTCAAACT
CAAAGGCCGCGACATCGAGCGCGTCGTGCTCGCCCGCGCGGTCAAATGGCATGTTGAAGATCGCGTTTTGATCTATGGAA
ATAGGACCGTAGTTTTTGTTTGA

Upstream 100 bases:

>100_bases
AATAGGAGAGTGCATAGGTCAAAGGCGAACTGCGCCGCGATCGTACTTTGGTGCAGGACACTCGTCCATGCACACGCGCA
TCCGGGCGATTTCGGCTATC

Downstream 100 bases:

>100_bases
GTTTATCTTGGCACTGAATCCTGTACTTTAAACATTTTCGCAGGCGGCAAATTGGCAGGAAATTTGGCTCCGTATCGGAT
GACCTGTCTTGCCGCATTGC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MLVLLPPDCPAPDTAMSPATRTLLISCPDRRGIVAAVSQCVLEAGGNILRSDQHTTDPLGGIFFMRLEFLYANAPADDDG
FARCFAPVAERFAMDWRLVRTGEPKRMALFVSRLDHCFVDLLWRRQSGELPVKIPLVVSNHPDLEPVAAQYGLPYHYLAI
DKTNQPAREAQMLNLLEGEVDFIVLARYMRVLSPQFVERYAGRIINIHHSFLPAFVGASPYERACERGVKVIGATAHYVT
EELDAGPIIEQDVVRVNHRDQVADLKLKGRDIERVVLARAVKWHVEDRVLIYGNRTVVFV

Sequences:

>Translated_300_residues
MLVLLPPDCPAPDTAMSPATRTLLISCPDRRGIVAAVSQCVLEAGGNILRSDQHTTDPLGGIFFMRLEFLYANAPADDDG
FARCFAPVAERFAMDWRLVRTGEPKRMALFVSRLDHCFVDLLWRRQSGELPVKIPLVVSNHPDLEPVAAQYGLPYHYLAI
DKTNQPAREAQMLNLLEGEVDFIVLARYMRVLSPQFVERYAGRIINIHHSFLPAFVGASPYERACERGVKVIGATAHYVT
EELDAGPIIEQDVVRVNHRDQVADLKLKGRDIERVVLARAVKWHVEDRVLIYGNRTVVFV
>Mature_300_residues
MLVLLPPDCPAPDTAMSPATRTLLISCPDRRGIVAAVSQCVLEAGGNILRSDQHTTDPLGGIFFMRLEFLYANAPADDDG
FARCFAPVAERFAMDWRLVRTGEPKRMALFVSRLDHCFVDLLWRRQSGELPVKIPLVVSNHPDLEPVAAQYGLPYHYLAI
DKTNQPAREAQMLNLLEGEVDFIVLARYMRVLSPQFVERYAGRIINIHHSFLPAFVGASPYERACERGVKVIGATAHYVT
EELDAGPIIEQDVVRVNHRDQVADLKLKGRDIERVVLARAVKWHVEDRVLIYGNRTVVFV

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=199, Percent_Identity=31.1557788944724, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI209869995, Length=199, Percent_Identity=31.1557788944724, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI209869993, Length=199, Percent_Identity=31.1557788944724, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1787483, Length=293, Percent_Identity=37.8839590443686, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788846, Length=114, Percent_Identity=38.5964912280702, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=167, Percent_Identity=35.3293413173653, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24582400, Length=195, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33711; Mature: 33711

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVLLPPDCPAPDTAMSPATRTLLISCPDRRGIVAAVSQCVLEAGGNILRSDQHTTDPLG
CEEEECCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCHH
GIFFMRLEFLYANAPADDDGFARCFAPVAERFAMDWRLVRTGEPKRMALFVSRLDHCFVD
HHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHH
LLWRRQSGELPVKIPLVVSNHPDLEPVAAQYGLPYHYLAIDKTNQPAREAQMLNLLEGEV
HHHHCCCCCCCEEEEEEECCCCCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCH
DFIVLARYMRVLSPQFVERYAGRIINIHHSFLPAFVGASPYERACERGVKVIGATAHYVT
HHHHHHHHHHHHCHHHHHHHCCCEEEEHHHHHHHHHCCCHHHHHHHCCCEEEECHHHHHH
EELDAGPIIEQDVVRVNHRDQVADLKLKGRDIERVVLARAVKWHVEDRVLIYGNRTVVFV
HHCCCCCCCHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECEEEEEC
>Mature Secondary Structure
MLVLLPPDCPAPDTAMSPATRTLLISCPDRRGIVAAVSQCVLEAGGNILRSDQHTTDPLG
CEEEECCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCHH
GIFFMRLEFLYANAPADDDGFARCFAPVAERFAMDWRLVRTGEPKRMALFVSRLDHCFVD
HHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHH
LLWRRQSGELPVKIPLVVSNHPDLEPVAAQYGLPYHYLAIDKTNQPAREAQMLNLLEGEV
HHHHCCCCCCCEEEEEEECCCCCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCH
DFIVLARYMRVLSPQFVERYAGRIINIHHSFLPAFVGASPYERACERGVKVIGATAHYVT
HHHHHHHHHHHHCHHHHHHHCCCEEEEHHHHHHHHHCCCHHHHHHHCCCEEEECHHHHHH
EELDAGPIIEQDVVRVNHRDQVADLKLKGRDIERVVLARAVKWHVEDRVLIYGNRTVVFV
HHCCCCCCCHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]