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The map label for this gene is ebrB [H]

Identifier: 37523893

GI number: 37523893

Start: 4557322

End: 4557657

Strand: Direct

Name: ebrB [H]

Synonym: glr4324

Alternate gene names: 37523893

Gene position: 4557322-4557657 (Clockwise)

Preceding gene: 37523891

Following gene: 37523896

Centisome position: 97.82

GC content: 62.5

Gene sequence:

>336_bases
GTGACTTTGCATCCGTTAATCCTGCTGGCTGTCGCCATCGCCAGCGAGGTGATCGGCTCCAGCGCGCTCAAATTGTCGGA
AGGTTTCAGCCGTCCCCTGCCAAGTTTGCTGGTAGTACTGGGCTACGGCGCAGCGTTTTACTTGCTGGGTCTGACCCTCA
AGGCGATGCCGCTGAGCGTGGTTTACGCCATCTGGTCCGGGGTGGGCACGGCGGCCACCGCCTTCATCGGCGTCGTTCTC
TTCCGCGAAGTACTCGACGCGCCCCGCCTGATCGGCATCGCCCTCATCATCGTTGGCGTGCTCGTCTTGAATCTTTCGGG
CGGCGGCCGCCTCTGA

Upstream 100 bases:

>100_bases
TGCAGCCGTGGCTGGGAGTGACCGGTGCGGTATTTTCACCCGTTCCATCGCCGAAAGCGGTATGGTTTGGTCTGGCCCGT
CAGGGGCTTTTTGGGGGGAG

Downstream 100 bases:

>100_bases
TATTGATCTATCTGAGCCGCTCCTCAAAATCTCAGGCGTTGCAATCCAGGTAGGCGGTAAAGCCCTCGATATTTTCGGCA
TCGATAATGGCACCGGCCAA

Product: multidrug resistance protein EbrA-like protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 111; Mature: 110

Protein sequence:

>111_residues
MTLHPLILLAVAIASEVIGSSALKLSEGFSRPLPSLLVVLGYGAAFYLLGLTLKAMPLSVVYAIWSGVGTAATAFIGVVL
FREVLDAPRLIGIALIIVGVLVLNLSGGGRL

Sequences:

>Translated_111_residues
MTLHPLILLAVAIASEVIGSSALKLSEGFSRPLPSLLVVLGYGAAFYLLGLTLKAMPLSVVYAIWSGVGTAATAFIGVVL
FREVLDAPRLIGIALIIVGVLVLNLSGGGRL
>Mature_110_residues
TLHPLILLAVAIASEVIGSSALKLSEGFSRPLPSLLVVLGYGAAFYLLGLTLKAMPLSVVYAIWSGVGTAATAFIGVVLF
REVLDAPRLIGIALIIVGVLVLNLSGGGRL

Specific function: Part of a multidrug efflux pump. Confers resistance to cationic lipophilic dyes such as ethidium bromide, acriflavine, pyronine Y and safranin O. The efflux is probably coupled to an influx of protons [H]

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family. EbrA/ebrB (TC 2.A.7.1.6) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786755, Length=103, Percent_Identity=44.6601941747573, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI87082378, Length=100, Percent_Identity=37, Blast_Score=65, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000390 [H]

Pfam domain/function: PF00893 Multi_Drug_Res [H]

EC number: NA

Molecular weight: Translated: 11429; Mature: 11298

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLHPLILLAVAIASEVIGSSALKLSEGFSRPLPSLLVVLGYGAAFYLLGLTLKAMPLSV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYAIWSGVGTAATAFIGVVLFREVLDAPRLIGIALIIVGVLVLNLSGGGRL
HHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TLHPLILLAVAIASEVIGSSALKLSEGFSRPLPSLLVVLGYGAAFYLLGLTLKAMPLSV
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYAIWSGVGTAATAFIGVVLFREVLDAPRLIGIALIIVGVLVLNLSGGGRL
HHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA