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The map label for this gene is nfi

Identifier: 37523812

GI number: 37523812

Start: 4460327

End: 4461067

Strand: Direct

Name: nfi

Synonym: glr4243

Alternate gene names: 37523812

Gene position: 4460327-4461067 (Clockwise)

Preceding gene: 37523809

Following gene: 37523814

Centisome position: 95.74

GC content: 65.45

Gene sequence:

>741_bases
GTGTCTGTCCCGAACGAACCCGACTCGAGTTTAGCCATGCATTCCTGGAATCTTACGCCTCAACAGGCGATCGAAGTGCA
AAAACAACTGGCCGTCCAGACCGTCCGCACGGGCAACCCCGAGGGGGTGCAACGCGTCGCCGGTGTGGATGTGAGCTTTA
ACCCCCGCGAGCCGAAAGCTCTAGTGCACGCGGTGGTGGTGGTGCTGAGCTATCCGGGCCTGGAGGTGGTCGACCGGCAG
GCGGTGAGTGCGGCGGTCGATTTTCCCTACATTCCGGGATTGTTGAGCTTTCGGGAGGCGCCACCGATTCTTGCCGCCAT
CGGCCAACTGTCCCAGAAGCCGGACCTGGTGATCGTGGATGGCCACGGCTACGCCCACCCGCGCCGTCTGGGGATCGCTT
CCCACCTGGGTCTGTTTCTCGATTTGCCCACGATCGGCTGCGCCAAATCGATCCTGGTGGGCCGTGCGGATGGGGATCTG
GCCGAAGCGGCCGGTTCGCTCACGGACCTGCTCTGGCGCGGCGAAGTGGTGGGGCGAGCCGTGCGCACCCGCAGCCGCGT
GCAGCCGGTCTATGTCTCCCCCGGACACCGCCTGGGTCTGGACAGTGCCGTCGAATGGGTGCTGCGCTGCTGCCGCGGGT
ACCGTTTGCCGGAGCCGACCCGCCAGGCCCACAACTACAGCAACCTGGTGCGCAAGGCGCGCCAGCCCATCAGCCTCAAC
GGCCTGAGCGGCGCAAAATAA

Upstream 100 bases:

>100_bases
CTGCGAGTGCTTTTTGCAACACCTGCCAGTTGTCGAACTTGCCGTCGCCCACGCAGCCGAACCGGCGGATGTCGGTCGCC
TCTTCTTTGAAAGTTTCCAA

Downstream 100 bases:

>100_bases
AGACTTCCTGGACGCCGACGGGCATCCAGCGCTCGACACGGTGCCGCTCGTAGATGGTGTAGCCGTCGATGGGTCCGAAA
GCAAAGACAAATTCGTCACG

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSVPNEPDSSLAMHSWNLTPQQAIEVQKQLAVQTVRTGNPEGVQRVAGVDVSFNPREPKALVHAVVVVLSYPGLEVVDRQ
AVSAAVDFPYIPGLLSFREAPPILAAIGQLSQKPDLVIVDGHGYAHPRRLGIASHLGLFLDLPTIGCAKSILVGRADGDL
AEAAGSLTDLLWRGEVVGRAVRTRSRVQPVYVSPGHRLGLDSAVEWVLRCCRGYRLPEPTRQAHNYSNLVRKARQPISLN
GLSGAK

Sequences:

>Translated_246_residues
MSVPNEPDSSLAMHSWNLTPQQAIEVQKQLAVQTVRTGNPEGVQRVAGVDVSFNPREPKALVHAVVVVLSYPGLEVVDRQ
AVSAAVDFPYIPGLLSFREAPPILAAIGQLSQKPDLVIVDGHGYAHPRRLGIASHLGLFLDLPTIGCAKSILVGRADGDL
AEAAGSLTDLLWRGEVVGRAVRTRSRVQPVYVSPGHRLGLDSAVEWVLRCCRGYRLPEPTRQAHNYSNLVRKARQPISLN
GLSGAK
>Mature_245_residues
SVPNEPDSSLAMHSWNLTPQQAIEVQKQLAVQTVRTGNPEGVQRVAGVDVSFNPREPKALVHAVVVVLSYPGLEVVDRQA
VSAAVDFPYIPGLLSFREAPPILAAIGQLSQKPDLVIVDGHGYAHPRRLGIASHLGLFLDLPTIGCAKSILVGRADGDLA
EAAGSLTDLLWRGEVVGRAVRTRSRVQPVYVSPGHRLGLDSAVEWVLRCCRGYRLPEPTRQAHNYSNLVRKARQPISLNG
LSGAK

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=200, Percent_Identity=41, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI257467548, Length=195, Percent_Identity=34.8717948717949, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI257467550, Length=195, Percent_Identity=34.8717948717949, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI87082357, Length=178, Percent_Identity=50.561797752809, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_GLOVI (Q7NDJ2)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_927189.1
- ProteinModelPortal:   Q7NDJ2
- GeneID:   2602646
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:glr4243
- NMPDR:   fig|251221.1.peg.4243
- HOGENOM:   HBG716360
- OMA:   MPYIPGL
- ProtClustDB:   CLSK647959
- BioCyc:   GVIO251221:GLR4243-MONOMER
- BRENDA:   3.1.21.7
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 26549; Mature: 26418

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPNEPDSSLAMHSWNLTPQQAIEVQKQLAVQTVRTGNPEGVQRVAGVDVSFNPREPKA
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHH
LVHAVVVVLSYPGLEVVDRQAVSAAVDFPYIPGLLSFREAPPILAAIGQLSQKPDLVIVD
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHCCCCCEEEEC
GHGYAHPRRLGIASHLGLFLDLPTIGCAKSILVGRADGDLAEAAGSLTDLLWRGEVVGRA
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHH
VRTRSRVQPVYVSPGHRLGLDSAVEWVLRCCRGYRLPEPTRQAHNYSNLVRKARQPISLN
HHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEC
GLSGAK
CCCCCC
>Mature Secondary Structure 
SVPNEPDSSLAMHSWNLTPQQAIEVQKQLAVQTVRTGNPEGVQRVAGVDVSFNPREPKA
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHH
LVHAVVVVLSYPGLEVVDRQAVSAAVDFPYIPGLLSFREAPPILAAIGQLSQKPDLVIVD
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHCCCCCEEEEC
GHGYAHPRRLGIASHLGLFLDLPTIGCAKSILVGRADGDLAEAAGSLTDLLWRGEVVGRA
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHH
VRTRSRVQPVYVSPGHRLGLDSAVEWVLRCCRGYRLPEPTRQAHNYSNLVRKARQPISLN
HHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEC
GLSGAK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292