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The map label for this gene is walK [H]

Identifier: 37523781

GI number: 37523781

Start: 4409534

End: 4411594

Strand: Direct

Name: walK [H]

Synonym: glr4212

Alternate gene names: 37523781

Gene position: 4409534-4411594 (Clockwise)

Preceding gene: 37523780

Following gene: 37523782

Centisome position: 94.65

GC content: 62.49

Gene sequence:

>2061_bases
ATGAACGAATCCTCCCCCTCGCCCGCCAACCTGCACGCCACGCTGCTCAAGGAGCTGGCGGACATCAAGTTTGCTCTCGA
TCAATCGTCGATTGTGGCGATCACCGACGCGGCGGGGCTGATCGTCTATGTCAACGACAAATTCTGCGAAATCTCACAGT
ACAGCCGCGCCGAGCTTTTGGGCCAGAACCACCGGATTGTCAACTCCGGCTTCCATCCGTCGGCATTTTTCGAGAACCTC
TGGCAGACGATCGCGGGGGGCAAAGTCTGGAGAGGCGAAATCCGCAATCGGGCCAAGGACGGCTCAATCTACTGGGTGGA
CACGACGATCGTGCCGTTTCTAGACCACCGCGGTCACTCCTACCAGTACGTGTCCATCCGCAACGACATCACCGAGCGCA
AGCGCGTCGAGGAAGCCCTGCGCCAGGCCAAAGACGAACTTGAAAGGCGCGTCGCCGAGCGTACCGACGACCTCAGCACC
GCCAACGCCCGGCTGCATGGTGAACTGGCCGAGCGCCGCCGCGCCGAAAAAGCGATCGAACTGTTGAGCCACCAAAACGA
ACTGATCTTGAATTCGGCGGGCGAGGGGATCGTGGGCCTCGATCTGCAGGGGCGGGTGGCGTTCGTCAACCCGGCCGGGG
CGCGCATGCTGGGCTGCGAGGTGCACGAACTGCTGGGCCAACCCTTTCACGAGCGGTTGCCCGGTGGGTGGACTGCGCCA
CCCGCCTATCGGATGAGCGACATCCCGCTTTACGCGTCGCTTATCGACGCCTCTGTGCACCGGGTGGGCAGTATTTGCTT
CGGCCGCCGGGACGGCTCGTCATTTCCGGTCGAGTACGTGAGCACCCCGATTCTCGAGCAGGGGCAAACCCTGGGGGCGG
TGATCACCTTTCGCGACATTACCGAAAGGCAGGCCGTCGAGCGGCTCAAAGCCGAATTTGTCTCGGTGGTGAGCCACGAA
CTGCGCACCCCGCTCACCTCTATCCGCGGGGCACTCGGGTTGCTCACCGGCGGGTTGTTGGGGCCGCTGTCCGAAAAAGG
CCGGCGCATGCTCGATATTGCCATTGCCAACACCGACCGGCTGGTGCGGCTTATCAACGACATCCTCGACGTCGAGCGCA
TGGAATCGGGCAAGGTGATTCTGGTGCGCCGGCGCTGCGATCTGGCCGAATTGCTCACCCAATCGGCGGACCTGATGCAG
TCAATGGCCCAGCAAGGCGGGGTAATGCTGCTCTGTGAGAGCAAATCCGCACCGCTCTGGGCCGATCCCGACCGCCTTAT
CCAGACATTTACCAATTTGATCAGCAATGCCATCAAGTTCTCTCCCGCGGGTTCGACGGTGCGGGTGAGCGCCGCGTGCG
ATGGGCCGCAGGTGCAGGTGCGGGTCGTCGACCAAGGGCGCGGCATCCCGGCGGAAAAACTCGAAAGCATCTTCGAGCGA
TTCCAGCAGGTCGACGCGAGCGATTCGCGCAAAAAAGGCGGCACCGGCCTGGGTCTGGCGATCTGCCGCAGCATCGTCTT
GCAGCACGGCGGTCGGCTCTGGGCGGAAAGTGCCCCCGGCAAAGGCAGCACCTTTGTGCTCACCCTGCCGTTGGCCGTCG
GCGAAACGCCCGCCCTTCCGGATACCTCCGCTTCTGCCGACATCCCCAATACGGATGAGAGTGCTTTGAAGGTTTTGGTG
GTCGAGGACGATCTCGAACTGGCCCACGTCCTTACCGTGATGTTCGCCCGCTATGGCGTGCAGACTCTCCACGCCCGCAC
CGCATCCGAGGCGTTCGCTTTTTGTTTGGACCACCACCCCGACTTGTTGGTGCTCGGTCTGCTCCTGACCGACGGCGACG
GCTTCGCGGTCGTCAATCGCCTTAGGCAGCACGACCGTCTGCGGTTGATGCCGCTGGTGGTTTATACCGTCCAGGAAATC
GATGAAGCCCAGCGCGAACGGCTCAAACTCGGGCCGACCCTGTTTTTTACCAAAAGCCGCATCCCGCCTGTCGAGTTCGA
GCATCAGGTTTTAAGGTTGCTGGCGCGTATCGCCCGCGAGCAGGCCGGGGAGGCCGGATGA

Upstream 100 bases:

>100_bases
CCTGGGCCGGCGGAGCCGACGACTTTGTGGCCAAACCCGTCTCCGGTCCGAATCTGGCTATGCGCATTCTCAACCGTCTT
GAACGCAGACGCTCCCTGTA

Downstream 100 bases:

>100_bases
AGCGCATCCTGGTCATCGACGACGAGGCGGATATCCGCGAAGTGATTCAGCTGACTCTGGAATTGACCCGCGGTTGGGAA
ATTGTTGCTGCCGAGTCGGG

Product: two-component hybrid sensor and regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MNESSPSPANLHATLLKELADIKFALDQSSIVAITDAAGLIVYVNDKFCEISQYSRAELLGQNHRIVNSGFHPSAFFENL
WQTIAGGKVWRGEIRNRAKDGSIYWVDTTIVPFLDHRGHSYQYVSIRNDITERKRVEEALRQAKDELERRVAERTDDLST
ANARLHGELAERRRAEKAIELLSHQNELILNSAGEGIVGLDLQGRVAFVNPAGARMLGCEVHELLGQPFHERLPGGWTAP
PAYRMSDIPLYASLIDASVHRVGSICFGRRDGSSFPVEYVSTPILEQGQTLGAVITFRDITERQAVERLKAEFVSVVSHE
LRTPLTSIRGALGLLTGGLLGPLSEKGRRMLDIAIANTDRLVRLINDILDVERMESGKVILVRRRCDLAELLTQSADLMQ
SMAQQGGVMLLCESKSAPLWADPDRLIQTFTNLISNAIKFSPAGSTVRVSAACDGPQVQVRVVDQGRGIPAEKLESIFER
FQQVDASDSRKKGGTGLGLAICRSIVLQHGGRLWAESAPGKGSTFVLTLPLAVGETPALPDTSASADIPNTDESALKVLV
VEDDLELAHVLTVMFARYGVQTLHARTASEAFAFCLDHHPDLLVLGLLLTDGDGFAVVNRLRQHDRLRLMPLVVYTVQEI
DEAQRERLKLGPTLFFTKSRIPPVEFEHQVLRLLARIAREQAGEAG

Sequences:

>Translated_686_residues
MNESSPSPANLHATLLKELADIKFALDQSSIVAITDAAGLIVYVNDKFCEISQYSRAELLGQNHRIVNSGFHPSAFFENL
WQTIAGGKVWRGEIRNRAKDGSIYWVDTTIVPFLDHRGHSYQYVSIRNDITERKRVEEALRQAKDELERRVAERTDDLST
ANARLHGELAERRRAEKAIELLSHQNELILNSAGEGIVGLDLQGRVAFVNPAGARMLGCEVHELLGQPFHERLPGGWTAP
PAYRMSDIPLYASLIDASVHRVGSICFGRRDGSSFPVEYVSTPILEQGQTLGAVITFRDITERQAVERLKAEFVSVVSHE
LRTPLTSIRGALGLLTGGLLGPLSEKGRRMLDIAIANTDRLVRLINDILDVERMESGKVILVRRRCDLAELLTQSADLMQ
SMAQQGGVMLLCESKSAPLWADPDRLIQTFTNLISNAIKFSPAGSTVRVSAACDGPQVQVRVVDQGRGIPAEKLESIFER
FQQVDASDSRKKGGTGLGLAICRSIVLQHGGRLWAESAPGKGSTFVLTLPLAVGETPALPDTSASADIPNTDESALKVLV
VEDDLELAHVLTVMFARYGVQTLHARTASEAFAFCLDHHPDLLVLGLLLTDGDGFAVVNRLRQHDRLRLMPLVVYTVQEI
DEAQRERLKLGPTLFFTKSRIPPVEFEHQVLRLLARIAREQAGEAG
>Mature_686_residues
MNESSPSPANLHATLLKELADIKFALDQSSIVAITDAAGLIVYVNDKFCEISQYSRAELLGQNHRIVNSGFHPSAFFENL
WQTIAGGKVWRGEIRNRAKDGSIYWVDTTIVPFLDHRGHSYQYVSIRNDITERKRVEEALRQAKDELERRVAERTDDLST
ANARLHGELAERRRAEKAIELLSHQNELILNSAGEGIVGLDLQGRVAFVNPAGARMLGCEVHELLGQPFHERLPGGWTAP
PAYRMSDIPLYASLIDASVHRVGSICFGRRDGSSFPVEYVSTPILEQGQTLGAVITFRDITERQAVERLKAEFVSVVSHE
LRTPLTSIRGALGLLTGGLLGPLSEKGRRMLDIAIANTDRLVRLINDILDVERMESGKVILVRRRCDLAELLTQSADLMQ
SMAQQGGVMLLCESKSAPLWADPDRLIQTFTNLISNAIKFSPAGSTVRVSAACDGPQVQVRVVDQGRGIPAEKLESIFER
FQQVDASDSRKKGGTGLGLAICRSIVLQHGGRLWAESAPGKGSTFVLTLPLAVGETPALPDTSASADIPNTDESALKVLV
VEDDLELAHVLTVMFARYGVQTLHARTASEAFAFCLDHHPDLLVLGLLLTDGDGFAVVNRLRQHDRLRLMPLVVYTVQEI
DEAQRERLKLGPTLFFTKSRIPPVEFEHQVLRLLARIAREQAGEAG

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788713, Length=324, Percent_Identity=29.6296296296296, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI48994928, Length=362, Percent_Identity=34.5303867403315, Blast_Score=140, Evalue=4e-34,
Organism=Escherichia coli, GI1788549, Length=363, Percent_Identity=27.5482093663912, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789149, Length=236, Percent_Identity=36.4406779661017, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87081816, Length=352, Percent_Identity=31.5340909090909, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI145693157, Length=232, Percent_Identity=37.0689655172414, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1786783, Length=267, Percent_Identity=30.3370786516854, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788393, Length=226, Percent_Identity=30.0884955752212, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1786600, Length=236, Percent_Identity=32.2033898305085, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=33.3333333333333, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1786912, Length=226, Percent_Identity=31.4159292035398, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1790346, Length=256, Percent_Identity=32.421875, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI87082128, Length=273, Percent_Identity=27.8388278388278, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1790861, Length=188, Percent_Identity=30.3191489361702, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1789453, Length=108, Percent_Identity=35.1851851851852, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787894, Length=224, Percent_Identity=31.25, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1790300, Length=371, Percent_Identity=26.1455525606469, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788279, Length=225, Percent_Identity=26.2222222222222, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1790551, Length=219, Percent_Identity=28.7671232876712, Blast_Score=64, Evalue=4e-11,
Organism=Escherichia coli, GI1789403, Length=200, Percent_Identity=30.5, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 75563; Mature: 75563

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNESSPSPANLHATLLKELADIKFALDQSSIVAITDAAGLIVYVNDKFCEISQYSRAELL
CCCCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHC
GQNHRIVNSGFHPSAFFENLWQTIAGGKVWRGEIRNRAKDGSIYWVDTTIVPFLDHRGHS
CCCCEEECCCCCHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCEEEEECEEEHHHCCCCCC
YQYVSIRNDITERKRVEEALRQAKDELERRVAERTDDLSTANARLHGELAERRRAEKAIE
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LLSHQNELILNSAGEGIVGLDLQGRVAFVNPAGARMLGCEVHELLGQPFHERLPGGWTAP
HHHCCCCEEEECCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCHHHHCCCCCCCC
PAYRMSDIPLYASLIDASVHRVGSICFGRRDGSSFPVEYVSTPILEQGQTLGAVITFRDI
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHCCCCEEEEEEEHHH
TERQAVERLKAEFVSVVSHELRTPLTSIRGALGLLTGGLLGPLSEKGRRMLDIAIANTDR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHCCCEEEEEEECCHHH
LVRLINDILDVERMESGKVILVRRRCDLAELLTQSADLMQSMAQQGGVMLLCESKSAPLW
HHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
ADPDRLIQTFTNLISNAIKFSPAGSTVRVSAACDGPQVQVRVVDQGRGIPAEKLESIFER
CCHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHH
FQQVDASDSRKKGGTGLGLAICRSIVLQHGGRLWAESAPGKGSTFVLTLPLAVGETPALP
HHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEEEECCCCCCCC
DTSASADIPNTDESALKVLVVEDDLELAHVLTVMFARYGVQTLHARTASEAFAFCLDHHP
CCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCC
DLLVLGLLLTDGDGFAVVNRLRQHDRLRLMPLVVYTVQEIDEAQRERLKLGPTLFFTKSR
CCEEEEEHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
IPPVEFEHQVLRLLARIAREQAGEAG
CCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNESSPSPANLHATLLKELADIKFALDQSSIVAITDAAGLIVYVNDKFCEISQYSRAELL
CCCCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHC
GQNHRIVNSGFHPSAFFENLWQTIAGGKVWRGEIRNRAKDGSIYWVDTTIVPFLDHRGHS
CCCCEEECCCCCHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCEEEEECEEEHHHCCCCCC
YQYVSIRNDITERKRVEEALRQAKDELERRVAERTDDLSTANARLHGELAERRRAEKAIE
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LLSHQNELILNSAGEGIVGLDLQGRVAFVNPAGARMLGCEVHELLGQPFHERLPGGWTAP
HHHCCCCEEEECCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCHHHHCCCCCCCC
PAYRMSDIPLYASLIDASVHRVGSICFGRRDGSSFPVEYVSTPILEQGQTLGAVITFRDI
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHCCCCEEEEEEEHHH
TERQAVERLKAEFVSVVSHELRTPLTSIRGALGLLTGGLLGPLSEKGRRMLDIAIANTDR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHCCCEEEEEEECCHHH
LVRLINDILDVERMESGKVILVRRRCDLAELLTQSADLMQSMAQQGGVMLLCESKSAPLW
HHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
ADPDRLIQTFTNLISNAIKFSPAGSTVRVSAACDGPQVQVRVVDQGRGIPAEKLESIFER
CCHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHH
FQQVDASDSRKKGGTGLGLAICRSIVLQHGGRLWAESAPGKGSTFVLTLPLAVGETPALP
HHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEEEECCCCCCCC
DTSASADIPNTDESALKVLVVEDDLELAHVLTVMFARYGVQTLHARTASEAFAFCLDHHP
CCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCC
DLLVLGLLLTDGDGFAVVNRLRQHDRLRLMPLVVYTVQEIDEAQRERLKLGPTLFFTKSR
CCEEEEEHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
IPPVEFEHQVLRLLARIAREQAGEAG
CCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA