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The map label for this gene is leuC1 [H]

Identifier: 37523656

GI number: 37523656

Start: 4284516

End: 4286453

Strand: Direct

Name: leuC1 [H]

Synonym: glr4087

Alternate gene names: 37523656

Gene position: 4284516-4286453 (Clockwise)

Preceding gene: 37523654

Following gene: 37523658

Centisome position: 91.96

GC content: 65.07

Gene sequence:

>1938_bases
ATGAATCTGACCTGTAAAATCTTGCAAGCCCACCTGATGAGCGGCGAATTGCGGCCAGGCTCCGAAATCGGCATCCGCAT
CGACCAGACACTGACCCAGGATGCCACCGGCACGATGGCCTATCTACAGTTCGAAGCGATGGGATTGCCGCGGGTGCGCA
CGAAGCTGTCGGTTTCCTACATCGACCACAACATGTTGCAGACGGGCTTCGAGAACGCCGACGATCATCGCTATTTGCAG
TCGGTGGGTCAGAAATACGGCATCGTCTTCTCGCGCCCGGGCAACGGCATCTGCCACCAGGTGCACCGCGAGCGCTTCGC
GGTGCCCGCTCAGACGCTGCTGGGTTCCGATTCGCACACGCCCACCTGCGGCGGCCTCGGCATGCTCGCCATCGGCGCGG
GCGGGCTCGACGTGGCGGTGGCGATGGCGGGCGAGCCTTACTTTATTCCGATGCCGAAGGTGCTGGGGGTAAGACTGACC
GGCACCCTGCAGCCCTGGTGCTCGGGCAAGGACGTCATCTTGGAGATGCTCCGGCGGCTCACCGTCAAGGGCGGCACCGG
CCGCATCGTCGAATATACCGGTCCGGGGGTCGCCTCGCTGAGCGTCTCCCATCGCTTCACGATCACCAACATGGGTGCCG
AACTCGGGGCAACCACCAGTGTCTTTCCTTCCGACGAAGTCACCCATCGCTTCCTGGCCGCCCAGGGCCGCGAAGGCGAC
TGGGTGCCCCTCGCCGCCGATCCGGACGCCACCTACGACGAAACGGTCGAAATCGACCTCGACACCCTGGAGCCGCTGAC
CGCCTGTCCCTCCAGCCCCGACAACGTCGTGAGCGTGCGCGCAGTGGCGGGGACACCCGTGGTGCAGGTGTGCATCGGCT
CGTGCACCAACTCCAGCTACGAAGATCTGGCCACCGCCGCGCTGTTGCTGCGCGGCAGGCGCGTGCATCCGAACGTCTCG
ATGACGGTCACCCCGGGTTCCAAGCAGGTCTTCGAGATGATCGTCCGCGACGGCTATTTCGCGGACTTGATTGCCGCCGG
TTGCCGCATTCTCGAATCGGCCTGCGGGCCGTGCATCGGCATGGGTCAGGCCCCTCCCACCGGCGGTGTCTCGGTGCGCA
GCTTCAACCGCAACTTCAAGGGCCGCTCCGGCACCGCCGACGATCTGGTCTATCTGGCGAGCCCCGAAGTCTGCGTCGCC
TGTGCCCTCACCGGCGAGATCACCGACCCGCGCACGCTGGGCATCGATCCGATTGCCATGGCCACCCCCGGGCAGTTCCT
CATCAACGACAACATGCTCGTCTTCCCGCCCGCCGACGGCAAAGACGTCGAGGTGCTGCGCGGTCCCAACATCAAGCCGG
TGCCGCTTGGCGAACCGCCCACCGCTACGCTGGAGGCGCCCGTTTTATTGAAACTGGGGGACAATATTTCCACCGACCAC
ATCATGCCGGCGGGGGCCAAGGTTTTGCCTTTGCGCTCCAATATCCCGGCCATCGCCGAGTTCGTCTTTATGCGCGTCGA
TCCGACTTTTCCTGAGCGCGCCCGGGCCGCCGGCCCTTCGTTGGTCGTGGGCGGGAGCAACTACGGCCAGGGTTCCTCGC
GCGAACACGCCGCCCTCGCCCCGATGTACCTGGGCGTGCGGGCGGTGATCGTCAAGTCCTTCGCCCGCATCCACCGCGCC
AACTTGATCAACTTCGGCATCCTGCCGCTCACCTTTGCGGACGAGGCCGACCACGACGGGGTCGATCAAGGCGACCAGTT
GCGCCTCGAAGGGATTGCCGAGGGTCTGACGACCGGCCACTTCACCGTCGCCAACCTCACCAAGGGCACGAACTTCCGGG
CCGACGCCCAGCTCACCGAGCGCGAGCGCGAAGTGATCCTGGCGGGCGGTCTGCTCAACAAGATCAAAAGCGAAAAGACC
GAGACGGCGGCGGTATAA

Upstream 100 bases:

>100_bases
GGCGTTTCTTTCTGAAAATGGCAGCTTCCGATACGGATCTGGAGGCGCGCTTAGACTAGAAAGTGATGCCCAGACCCGCA
AGCGTAGCGAGACACCCGCG

Downstream 100 bases:

>100_bases
CAGCGCTAGGTTCAAAAAGTCGCGCGCTTTTTCAGCGCGCGCTTTCTCCACGCACCTGGGTGGCGCTGGCGCTGTCGGAG
TGGAATTTCGCAATCGTCTG

Product: aconitate hydratase

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 645; Mature: 645

Protein sequence:

>645_residues
MNLTCKILQAHLMSGELRPGSEIGIRIDQTLTQDATGTMAYLQFEAMGLPRVRTKLSVSYIDHNMLQTGFENADDHRYLQ
SVGQKYGIVFSRPGNGICHQVHRERFAVPAQTLLGSDSHTPTCGGLGMLAIGAGGLDVAVAMAGEPYFIPMPKVLGVRLT
GTLQPWCSGKDVILEMLRRLTVKGGTGRIVEYTGPGVASLSVSHRFTITNMGAELGATTSVFPSDEVTHRFLAAQGREGD
WVPLAADPDATYDETVEIDLDTLEPLTACPSSPDNVVSVRAVAGTPVVQVCIGSCTNSSYEDLATAALLLRGRRVHPNVS
MTVTPGSKQVFEMIVRDGYFADLIAAGCRILESACGPCIGMGQAPPTGGVSVRSFNRNFKGRSGTADDLVYLASPEVCVA
CALTGEITDPRTLGIDPIAMATPGQFLINDNMLVFPPADGKDVEVLRGPNIKPVPLGEPPTATLEAPVLLKLGDNISTDH
IMPAGAKVLPLRSNIPAIAEFVFMRVDPTFPERARAAGPSLVVGGSNYGQGSSREHAALAPMYLGVRAVIVKSFARIHRA
NLINFGILPLTFADEADHDGVDQGDQLRLEGIAEGLTTGHFTVANLTKGTNFRADAQLTEREREVILAGGLLNKIKSEKT
ETAAV

Sequences:

>Translated_645_residues
MNLTCKILQAHLMSGELRPGSEIGIRIDQTLTQDATGTMAYLQFEAMGLPRVRTKLSVSYIDHNMLQTGFENADDHRYLQ
SVGQKYGIVFSRPGNGICHQVHRERFAVPAQTLLGSDSHTPTCGGLGMLAIGAGGLDVAVAMAGEPYFIPMPKVLGVRLT
GTLQPWCSGKDVILEMLRRLTVKGGTGRIVEYTGPGVASLSVSHRFTITNMGAELGATTSVFPSDEVTHRFLAAQGREGD
WVPLAADPDATYDETVEIDLDTLEPLTACPSSPDNVVSVRAVAGTPVVQVCIGSCTNSSYEDLATAALLLRGRRVHPNVS
MTVTPGSKQVFEMIVRDGYFADLIAAGCRILESACGPCIGMGQAPPTGGVSVRSFNRNFKGRSGTADDLVYLASPEVCVA
CALTGEITDPRTLGIDPIAMATPGQFLINDNMLVFPPADGKDVEVLRGPNIKPVPLGEPPTATLEAPVLLKLGDNISTDH
IMPAGAKVLPLRSNIPAIAEFVFMRVDPTFPERARAAGPSLVVGGSNYGQGSSREHAALAPMYLGVRAVIVKSFARIHRA
NLINFGILPLTFADEADHDGVDQGDQLRLEGIAEGLTTGHFTVANLTKGTNFRADAQLTEREREVILAGGLLNKIKSEKT
ETAAV
>Mature_645_residues
MNLTCKILQAHLMSGELRPGSEIGIRIDQTLTQDATGTMAYLQFEAMGLPRVRTKLSVSYIDHNMLQTGFENADDHRYLQ
SVGQKYGIVFSRPGNGICHQVHRERFAVPAQTLLGSDSHTPTCGGLGMLAIGAGGLDVAVAMAGEPYFIPMPKVLGVRLT
GTLQPWCSGKDVILEMLRRLTVKGGTGRIVEYTGPGVASLSVSHRFTITNMGAELGATTSVFPSDEVTHRFLAAQGREGD
WVPLAADPDATYDETVEIDLDTLEPLTACPSSPDNVVSVRAVAGTPVVQVCIGSCTNSSYEDLATAALLLRGRRVHPNVS
MTVTPGSKQVFEMIVRDGYFADLIAAGCRILESACGPCIGMGQAPPTGGVSVRSFNRNFKGRSGTADDLVYLASPEVCVA
CALTGEITDPRTLGIDPIAMATPGQFLINDNMLVFPPADGKDVEVLRGPNIKPVPLGEPPTATLEAPVLLKLGDNISTDH
IMPAGAKVLPLRSNIPAIAEFVFMRVDPTFPERARAAGPSLVVGGSNYGQGSSREHAALAPMYLGVRAVIVKSFARIHRA
NLINFGILPLTFADEADHDGVDQGDQLRLEGIAEGLTTGHFTVANLTKGTNFRADAQLTEREREVILAGGLLNKIKSEKT
ETAAV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=720, Percent_Identity=37.7777777777778, Blast_Score=348, Evalue=1e-95,
Organism=Homo sapiens, GI8659555, Length=712, Percent_Identity=29.3539325842697, Blast_Score=223, Evalue=4e-58,
Organism=Homo sapiens, GI41352693, Length=714, Percent_Identity=27.8711484593838, Blast_Score=179, Evalue=6e-45,
Organism=Escherichia coli, GI87081781, Length=732, Percent_Identity=28.1420765027322, Blast_Score=234, Evalue=9e-63,
Organism=Escherichia coli, GI1786259, Length=442, Percent_Identity=29.4117647058824, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1787531, Length=625, Percent_Identity=28, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI2367097, Length=411, Percent_Identity=26.7639902676399, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25149337, Length=722, Percent_Identity=37.2576177285319, Blast_Score=348, Evalue=5e-96,
Organism=Caenorhabditis elegans, GI32564738, Length=686, Percent_Identity=37.7551020408163, Blast_Score=341, Evalue=7e-94,
Organism=Caenorhabditis elegans, GI25149342, Length=572, Percent_Identity=37.2377622377622, Blast_Score=281, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI17568399, Length=800, Percent_Identity=26, Blast_Score=197, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6323335, Length=721, Percent_Identity=37.5866851595007, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6322261, Length=731, Percent_Identity=34.610123119015, Blast_Score=361, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6320440, Length=628, Percent_Identity=29.2993630573248, Blast_Score=202, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6321429, Length=661, Percent_Identity=26.7776096822995, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI281365315, Length=718, Percent_Identity=36.0724233983287, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI17864292, Length=718, Percent_Identity=36.0724233983287, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI28571643, Length=720, Percent_Identity=36.1111111111111, Blast_Score=326, Evalue=3e-89,
Organism=Drosophila melanogaster, GI161076999, Length=682, Percent_Identity=36.6568914956012, Blast_Score=322, Evalue=6e-88,
Organism=Drosophila melanogaster, GI24645686, Length=762, Percent_Identity=27.6902887139108, Blast_Score=176, Evalue=5e-44,
Organism=Drosophila melanogaster, GI17137564, Length=656, Percent_Identity=28.3536585365854, Blast_Score=171, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 68609; Mature: 68609

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTCKILQAHLMSGELRPGSEIGIRIDQTLTQDATGTMAYLQFEAMGLPRVRTKLSVSY
CCCCHHHHHHHHHCCCCCCCCCCCEEECCHHCCCCCCCEEEEEEEECCCCHHHHEEEEEH
IDHNMLQTGFENADDHRYLQSVGQKYGIVFSRPGNGICHQVHRERFAVPAQTLLGSDSHT
HHHHHHHHCCCCCCHHHHHHHHCHHCCEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCCC
PTCGGLGMLAIGAGGLDVAVAMAGEPYFIPMPKVLGVRLTGTLQPWCSGKDVILEMLRRL
CCCCCCCEEEECCCCCEEEEEECCCCEEEECHHHHCEEEEECCCCCCCCHHHHHHHHHHH
TVKGGTGRIVEYTGPGVASLSVSHRFTITNMGAELGATTSVFPSDEVTHRFLAAQGREGD
CCCCCCCCEEEECCCCEEEEEEEEEEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCCCC
WVPLAADPDATYDETVEIDLDTLEPLTACPSSPDNVVSVRAVAGTPVVQVCIGSCTNSSY
EEEEECCCCCCCCCEEEEECCCCCHHHCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCH
EDLATAALLLRGRRVHPNVSMTVTPGSKQVFEMIVRDGYFADLIAAGCRILESACGPCIG
HHHHHHHHHHCCCEECCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEE
MGQAPPTGGVSVRSFNRNFKGRSGTADDLVYLASPEVCVACALTGEITDPRTLGIDPIAM
CCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCEEE
ATPGQFLINDNMLVFPPADGKDVEVLRGPNIKPVPLGEPPTATLEAPVLLKLGDNISTDH
CCCCCEEEECCEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
IMPAGAKVLPLRSNIPAIAEFVFMRVDPTFPERARAAGPSLVVGGSNYGQGSSREHAALA
CCCCCCEEEEECCCCCHHHHEEEEEECCCCCHHHHHCCCEEEECCCCCCCCCCCCCHHHH
PMYLGVRAVIVKSFARIHRANLINFGILPLTFADEADHDGVDQGDQLRLEGIAEGLTTGH
HHHHHHHHHHHHHHHHHHHHCEEEECEEEEEECCCCCCCCCCCCCEEEHHHHHCCCCCCC
FTVANLTKGTNFRADAQLTEREREVILAGGLLNKIKSEKTETAAV
EEEEEECCCCCCCCCCHHHHCCCEEEEECHHHHHHHCCCHHHCCC
>Mature Secondary Structure
MNLTCKILQAHLMSGELRPGSEIGIRIDQTLTQDATGTMAYLQFEAMGLPRVRTKLSVSY
CCCCHHHHHHHHHCCCCCCCCCCCEEECCHHCCCCCCCEEEEEEEECCCCHHHHEEEEEH
IDHNMLQTGFENADDHRYLQSVGQKYGIVFSRPGNGICHQVHRERFAVPAQTLLGSDSHT
HHHHHHHHCCCCCCHHHHHHHHCHHCCEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCCC
PTCGGLGMLAIGAGGLDVAVAMAGEPYFIPMPKVLGVRLTGTLQPWCSGKDVILEMLRRL
CCCCCCCEEEECCCCCEEEEEECCCCEEEECHHHHCEEEEECCCCCCCCHHHHHHHHHHH
TVKGGTGRIVEYTGPGVASLSVSHRFTITNMGAELGATTSVFPSDEVTHRFLAAQGREGD
CCCCCCCCEEEECCCCEEEEEEEEEEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCCCC
WVPLAADPDATYDETVEIDLDTLEPLTACPSSPDNVVSVRAVAGTPVVQVCIGSCTNSSY
EEEEECCCCCCCCCEEEEECCCCCHHHCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCH
EDLATAALLLRGRRVHPNVSMTVTPGSKQVFEMIVRDGYFADLIAAGCRILESACGPCIG
HHHHHHHHHHCCCEECCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEE
MGQAPPTGGVSVRSFNRNFKGRSGTADDLVYLASPEVCVACALTGEITDPRTLGIDPIAM
CCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCEEE
ATPGQFLINDNMLVFPPADGKDVEVLRGPNIKPVPLGEPPTATLEAPVLLKLGDNISTDH
CCCCCEEEECCEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
IMPAGAKVLPLRSNIPAIAEFVFMRVDPTFPERARAAGPSLVVGGSNYGQGSSREHAALA
CCCCCCEEEEECCCCCHHHHEEEEEECCCCCHHHHHCCCEEEECCCCCCCCCCCCCHHHH
PMYLGVRAVIVKSFARIHRANLINFGILPLTFADEADHDGVDQGDQLRLEGIAEGLTTGH
HHHHHHHHHHHHHHHHHHHHCEEEECEEEEEECCCCCCCCCCCCCEEEHHHHHCCCCCCC
FTVANLTKGTNFRADAQLTEREREVILAGGLLNKIKSEKTETAAV
EEEEEECCCCCCCCCCHHHHCCCEEEEECHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]