Click here to switch to the map view.

The map label for this gene is yfjR [H]

Identifier: 37523328

GI number: 37523328

Start: 3968496

End: 3969368

Strand: Direct

Name: yfjR [H]

Synonym: glr3759

Alternate gene names: 37523328

Gene position: 3968496-3969368 (Clockwise)

Preceding gene: 37523326

Following gene: 37523332

Centisome position: 85.18

GC content: 62.89

Gene sequence:

>873_bases
ATGTCGAAAGCCGCCGTTTTGGGAATGGGTGCCATGGGCTCGCGTATGGCCGCAGCGCTACTGAAGGCAGGTCATCAAGT
GACCGTCTGGAACCGCAATTCGCAGAAAACCGCTCCGCTAGCCGAAATGGGAGCAAAAGCCGCCCAGACTCCTCGGGCGG
CCGTGGAGGAAGTCGATTTTGCCGTTTGCATGGTCCGCGACGACGCGGCCTCGCGCTTCGTCTGGCTCGATTTTGAGACC
GGGGCGCTTGCAGGTCTTCCGGCAGATGCTGTGGCCATCGAAAGCTCGACGCTGAGTGTCGCCGGGGTGCAGGAGCTTGC
TGAACACTTCCAGGCAAGGGGGAAGACTTTGCTGGACGCTCCGGTCTCGGGCTCCCGGCCCCAGGCGGAGGCGGGGCAGC
TGATTTTTCTTGTCGGCGGCGACGCCGGTGCGGTTGCGAAGGCGGGACCGATACTAAACGCCATGGGTGGGACAGTCCAC
CACGCCGGACCGCTTGGCAACGGGGCTGCCGTCAAGCTTGCAATCAACGCCCTGTTAGGAGTACAGGTGGCGGCCATGGG
CGAACTGATCGCACTGCTGCGCCACTGTGGGATCGACGAGGTCCGGGCTGTCGAAATCATTGGCTCGACCTCCGTTTGCA
GCCCCGCGGCCAGAGGTGCCGCAAGCGCTATGGCAGTGGGCAATTACGCGCCCTTATTTCCAAGCGCCCTGATGGAGAAA
GATCTCGGTTACTTGCAGAATATGGCTGCAGCGCACCGGGCGCGCGCTCCGCTGACCCAAGCTGCCCGAAGCGTATTTGG
CGAAGCGATGAGCCGGGGGTACGGCGAAGACAACATGACCGGTGTAGCCCAGCTGTACTGGCAAGGACCGTGA

Upstream 100 bases:

>100_bases
CCATTGCTGCATTAGCTGCACGAATACAATGCTTGGATCCTAGTTTATTTGTCTGCATGCACAGGATTAGGATGGCTTCG
AGAAGCAACGGAGCAAGCAC

Downstream 100 bases:

>100_bases
GCAGCGGTTGGAGACCAACAAAGAAGATGTTTTAAGGCCGTTTGCCGGCCGAGCTTGTGATCCAATCAGCTACATCATCG
ACGACAACCTGCACGACGTG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSKAAVLGMGAMGSRMAAALLKAGHQVTVWNRNSQKTAPLAEMGAKAAQTPRAAVEEVDFAVCMVRDDAASRFVWLDFET
GALAGLPADAVAIESSTLSVAGVQELAEHFQARGKTLLDAPVSGSRPQAEAGQLIFLVGGDAGAVAKAGPILNAMGGTVH
HAGPLGNGAAVKLAINALLGVQVAAMGELIALLRHCGIDEVRAVEIIGSTSVCSPAARGAASAMAVGNYAPLFPSALMEK
DLGYLQNMAAAHRARAPLTQAARSVFGEAMSRGYGEDNMTGVAQLYWQGP

Sequences:

>Translated_290_residues
MSKAAVLGMGAMGSRMAAALLKAGHQVTVWNRNSQKTAPLAEMGAKAAQTPRAAVEEVDFAVCMVRDDAASRFVWLDFET
GALAGLPADAVAIESSTLSVAGVQELAEHFQARGKTLLDAPVSGSRPQAEAGQLIFLVGGDAGAVAKAGPILNAMGGTVH
HAGPLGNGAAVKLAINALLGVQVAAMGELIALLRHCGIDEVRAVEIIGSTSVCSPAARGAASAMAVGNYAPLFPSALMEK
DLGYLQNMAAAHRARAPLTQAARSVFGEAMSRGYGEDNMTGVAQLYWQGP
>Mature_289_residues
SKAAVLGMGAMGSRMAAALLKAGHQVTVWNRNSQKTAPLAEMGAKAAQTPRAAVEEVDFAVCMVRDDAASRFVWLDFETG
ALAGLPADAVAIESSTLSVAGVQELAEHFQARGKTLLDAPVSGSRPQAEAGQLIFLVGGDAGAVAKAGPILNAMGGTVHH
AGPLGNGAAVKLAINALLGVQVAAMGELIALLRHCGIDEVRAVEIIGSTSVCSPAARGAASAMAVGNYAPLFPSALMEKD
LGYLQNMAAAHRARAPLTQAARSVFGEAMSRGYGEDNMTGVAQLYWQGP

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=285, Percent_Identity=29.8245614035088, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI23308751, Length=286, Percent_Identity=26.5734265734266, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI145693186, Length=286, Percent_Identity=30.4195804195804, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1790315, Length=290, Percent_Identity=31.3793103448276, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1786719, Length=286, Percent_Identity=25.8741258741259, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789092, Length=285, Percent_Identity=28.7719298245614, Blast_Score=81, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17557316, Length=274, Percent_Identity=25.9124087591241, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28574115, Length=277, Percent_Identity=31.0469314079422, Blast_Score=131, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24655230, Length=286, Percent_Identity=31.4685314685315, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI19922568, Length=286, Percent_Identity=31.4685314685315, Blast_Score=127, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 29647; Mature: 29516

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAAVLGMGAMGSRMAAALLKAGHQVTVWNRNSQKTAPLAEMGAKAAQTPRAAVEEVDF
CCCCEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHCCHHHCCHHHHHHHCCE
AVCMVRDDAASRFVWLDFETGALAGLPADAVAIESSTLSVAGVQELAEHFQARGKTLLDA
EEEEEECCCCCEEEEEEECCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEEC
PVSGSRPQAEAGQLIFLVGGDAGAVAKAGPILNAMGGTVHHAGPLGNGAAVKLAINALLG
CCCCCCCCCCCCCEEEEEECCCCCHHHCCCHHHHCCCCEECCCCCCCCCCHHHHHHHHHH
VQVAAMGELIALLRHCGIDEVRAVEIIGSTSVCSPAARGAASAMAVGNYAPLFPSALMEK
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHCHHHHHHCCCCCCCCHHHHHHH
DLGYLQNMAAAHRARAPLTQAARSVFGEAMSRGYGEDNMTGVAQLYWQGP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
SKAAVLGMGAMGSRMAAALLKAGHQVTVWNRNSQKTAPLAEMGAKAAQTPRAAVEEVDF
CCCEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHCCHHHCCHHHHHHHCCE
AVCMVRDDAASRFVWLDFETGALAGLPADAVAIESSTLSVAGVQELAEHFQARGKTLLDA
EEEEEECCCCCEEEEEEECCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEEC
PVSGSRPQAEAGQLIFLVGGDAGAVAKAGPILNAMGGTVHHAGPLGNGAAVKLAINALLG
CCCCCCCCCCCCCEEEEEECCCCCHHHCCCHHHHCCCCEECCCCCCCCCCHHHHHHHHHH
VQVAAMGELIALLRHCGIDEVRAVEIIGSTSVCSPAARGAASAMAVGNYAPLFPSALMEK
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHCHHHHHHCCCCCCCCHHHHHHH
DLGYLQNMAAAHRARAPLTQAARSVFGEAMSRGYGEDNMTGVAQLYWQGP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]