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The map label for this gene is b2999 [C]

Identifier: 37523309

GI number: 37523309

Start: 3951729

End: 3952421

Strand: Reverse

Name: b2999 [C]

Synonym: gll3740

Alternate gene names: 37523309

Gene position: 3952421-3951729 (Counterclockwise)

Preceding gene: 37523312

Following gene: 37523307

Centisome position: 84.83

GC content: 63.64

Gene sequence:

>693_bases
ATGGCGGAGCGCATCACCATCGACTCGAAGGACGGCAGCTTCGGCGCCTACGTGGCCAGACCGGGTGCGGGCTTCGGCCC
CGGCCTGGTGGTCATCCAGGAAATATTTGGCATCAACGCCACGATGCGGGCCATCGCCGACGCCTTTGCCCGGGCGGGCT
ACATCGCCGTGGTGCCGGATTTGTTCTGGCGGCTGGAGCCGGGAGTTGAACTGATTGACCAGGGCAGCGATCTCGAAAAA
GCCTTTGGACTCTACAACGCCTACGACGAAGATCTGGGCGTCACGGATCTGATTGCCGCCCTCGGTGCCGTCCGCCAACT
GGAAGGCTGCACCGGCAAGGTGGGCACGGTGGGCTACTGCCTGGGAGGCAAACTCGCGTATCTGATGGCCACCCGCTCCG
ACGCCGATGCCAACGTCAGCTACTACGGCGTCGGTATCGAACGCAACCTGGAGGAAGTGGGCAACATTTCCCGGCCACTG
CTGTTGCATATCGCCGGGGAGGACGAACTGGTCGCCCCGGCCGCCCGGGGGCGCATTCTGGATGCCGTGGCGCAAAATCC
GCTCATCACCACCTATCTCTATCCAGGTCTGGGCCACGCCTTTGCCCGCGTGGACGGCACCACCTATCAAAAGGAAGCCG
CCGAGCTGGCCAACGGCCGTACCCTCACATTTTTGAGCAGCCACCTGGGCTAG

Upstream 100 bases:

>100_bases
CCTGTACTTCAAGCTTGTCGTACCGTGGAGACGGATACCATCGGCGGATCGGCTAGCGGCCACCCGGAAGATTGGTTAGG
AAGTGGGGGAGACCAACAGC

Downstream 100 bases:

>100_bases
AGCTTTCAGCGGTTCACCAGTTCGCCTACCACCAAAACCGGCACCGTCTCCCCTTCGCAGGCACCCGTCACCCCGGCTTC
CAGCCAGGCAAAGGCGTTGA

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MAERITIDSKDGSFGAYVARPGAGFGPGLVVIQEIFGINATMRAIADAFARAGYIAVVPDLFWRLEPGVELIDQGSDLEK
AFGLYNAYDEDLGVTDLIAALGAVRQLEGCTGKVGTVGYCLGGKLAYLMATRSDADANVSYYGVGIERNLEEVGNISRPL
LLHIAGEDELVAPAARGRILDAVAQNPLITTYLYPGLGHAFARVDGTTYQKEAAELANGRTLTFLSSHLG

Sequences:

>Translated_230_residues
MAERITIDSKDGSFGAYVARPGAGFGPGLVVIQEIFGINATMRAIADAFARAGYIAVVPDLFWRLEPGVELIDQGSDLEK
AFGLYNAYDEDLGVTDLIAALGAVRQLEGCTGKVGTVGYCLGGKLAYLMATRSDADANVSYYGVGIERNLEEVGNISRPL
LLHIAGEDELVAPAARGRILDAVAQNPLITTYLYPGLGHAFARVDGTTYQKEAAELANGRTLTFLSSHLG
>Mature_229_residues
AERITIDSKDGSFGAYVARPGAGFGPGLVVIQEIFGINATMRAIADAFARAGYIAVVPDLFWRLEPGVELIDQGSDLEKA
FGLYNAYDEDLGVTDLIAALGAVRQLEGCTGKVGTVGYCLGGKLAYLMATRSDADANVSYYGVGIERNLEEVGNISRPLL
LHIAGEDELVAPAARGRILDAVAQNPLITTYLYPGLGHAFARVDGTTYQKEAAELANGRTLTFLSSHLG

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=237, Percent_Identity=25.3164556962025, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 24375; Mature: 24244

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERITIDSKDGSFGAYVARPGAGFGPGLVVIQEIFGINATMRAIADAFARAGYIAVVPD
CCCEEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEHHH
LFWRLEPGVELIDQGSDLEKAFGLYNAYDEDLGVTDLIAALGAVRQLEGCTGKVGTVGYC
HHHHCCCCHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
LGGKLAYLMATRSDADANVSYYGVGIERNLEEVGNISRPLLLHIAGEDELVAPAARGRIL
CCCEEEEEEEECCCCCCCEEEEECCHHCCHHHHCCCCCCEEEEECCCCCEECHHHHCHHH
DAVAQNPLITTYLYPGLGHAFARVDGTTYQKEAAELANGRTLTFLSSHLG
HHHHCCCEEEEEECCCCCHHHHHCCCCCHHHHHHHHHCCCEEEHHHHHCC
>Mature Secondary Structure 
AERITIDSKDGSFGAYVARPGAGFGPGLVVIQEIFGINATMRAIADAFARAGYIAVVPD
CCEEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEHHH
LFWRLEPGVELIDQGSDLEKAFGLYNAYDEDLGVTDLIAALGAVRQLEGCTGKVGTVGYC
HHHHCCCCHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
LGGKLAYLMATRSDADANVSYYGVGIERNLEEVGNISRPLLLHIAGEDELVAPAARGRIL
CCCEEEEEEEECCCCCCCEEEEECCHHCCHHHHCCCCCCEEEEECCCCCEECHHHHCHHH
DAVAQNPLITTYLYPGLGHAFARVDGTTYQKEAAELANGRTLTFLSSHLG
HHHHCCCEEEEEECCCCCHHHHHCCCCCHHHHHHHHHCCCEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8138139 [H]