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The map label for this gene is cobB

Identifier: 37523264

GI number: 37523264

Start: 3905624

End: 3906982

Strand: Direct

Name: cobB

Synonym: gvip500

Alternate gene names: 37523264

Gene position: 3905624-3906982 (Clockwise)

Preceding gene: 37523262

Following gene: 37523265

Centisome position: 83.83

GC content: 67.11

Gene sequence:

>1359_bases
ATGGCGATCATCATTGCAGCCCCGGCGAGCGGCACCGGCAAGACGACGATAACCCTCGCTCTGCTTGCCTACCTCAAGGC
CCACGCCTTGCGGGTGCGCTCGTTCAAGGTCGGCCCCGATTATATCGACCCGATGTTCCACGCCGCCGTCACCGGCCAGG
GCTGCGTCAATCTCGACTTGTTTTTGACCGACGAACCGTTCGTGCGCGCCACCTACCACCACCACTGCCGGGGCCAGCAG
GCGGCCGTGATCGAAGGGGTAATGGGGCTATTCGACGGACGGGCGGGTCAGGGCGATTTTGCCAGCACCGCCCACGTCGC
CCGCCTGTTGCGCTTGCCGGTGATCCTGGTCATCGATGGATCGGGGGCCGGCTTTTCGGTGGCGGCCACCCTCTACGGAT
TTCGCCGCTTCGACCCGCGCGTGCGCATCGCCGGGGTGATCCTCAACCGGGTGGGCTCCGAGCGCCACGCCCAAATCCTC
TCTGAGGCTGTGCGCAGCCAGGGTCTCGAACTATTGGGAACCGTCTACAAAGACGAAACGATCGCCCTGCCGCACCGCCA
CCTGGGATTGGTGCCGGTGGAGGAACTGTCCGACTTTCGGCGCACCCAGCAGCAGTTGGCTGCCCTCGCCGAGCGCTCCT
TCGACTGGCAGAAATTGCTGCCGCTGGTGGCCATCCAACCTACCGCCCCCGCCCCGGCGCGCTGGGAAGCGGAGCCATTG
CCGCCGGTGCGCATCGCCGTCGCCCGCGACCGGGCCTTCAGCTTCTACTACCAGGACAATCTGGAATTGCTCGCGGCCCT
GGGGGCGCAGCTGGAGACCTTTTCGCCTTTGGCCGGCGAGTGGCCCGATTGCCGGGGTTACTTGTTGGGAGGCGGTTTTC
CCGAGCTGTGCGCGGGCGAACTCAGCGCAAAAACCCGCTTCTGGGAAGGACTGCGGGGGGCGGTGGCCCGTGGGGTGCCC
CTCTATGCCGAGTGCGGCGGGTTGATGGTGCTGGGCGAAGCGCTGCGCACCCCCGAGGGCCGCTCCCACCCGATGGCGGG
CATCCTGGAGGCCAGTTGCTGGATGGGCAAACGCACGGTACTGGGCTACCGGGTGGCCACGGCTTTGCACAGTTCCTGCG
CCGTCGAAGCCGGCAGACAGTTGCGCGGCCACCTGTTTCACCGCTCGCGCATGGAACCCCAGCCGGGCGCGCCGCTGTGG
CAACTCGACGACGATGAGCAAGAAGGCTGGGTGCGCGGCAATCTGCACGCTTCGTATCTGCACCTGCACTGGGGTACCCA
GGCGTGGGCGGCCCGCCGCTTCGTGCGCCGCTGTCTGGCGGTCGCTTTCGAGGGAAAATCCACCCCGGCCCGCTTCTGA

Upstream 100 bases:

>100_bases
TGCGCAACAATTTGGACCACCTGTCTCCCTGTTCAACGTGGCAGCCACCCGGATTTTGCCCATAGACTGAAGAGCGGTTT
TTCACCGGACGACAGGCTCT

Downstream 100 bases:

>100_bases
AGGCCACCGGTTGATAGGATAGTCGCCAAACGGTTTGCGCTGAGCGGAGGCCCGGGTGCTCCTATCGATCCTGCGGGAAG
TAGTCGCCGATGGCCTGCTC

Product: cobyrinic acid a,c-diamide synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MAIIIAAPASGTGKTTITLALLAYLKAHALRVRSFKVGPDYIDPMFHAAVTGQGCVNLDLFLTDEPFVRATYHHHCRGQQ
AAVIEGVMGLFDGRAGQGDFASTAHVARLLRLPVILVIDGSGAGFSVAATLYGFRRFDPRVRIAGVILNRVGSERHAQIL
SEAVRSQGLELLGTVYKDETIALPHRHLGLVPVEELSDFRRTQQQLAALAERSFDWQKLLPLVAIQPTAPAPARWEAEPL
PPVRIAVARDRAFSFYYQDNLELLAALGAQLETFSPLAGEWPDCRGYLLGGGFPELCAGELSAKTRFWEGLRGAVARGVP
LYAECGGLMVLGEALRTPEGRSHPMAGILEASCWMGKRTVLGYRVATALHSSCAVEAGRQLRGHLFHRSRMEPQPGAPLW
QLDDDEQEGWVRGNLHASYLHLHWGTQAWAARRFVRRCLAVAFEGKSTPARF

Sequences:

>Translated_452_residues
MAIIIAAPASGTGKTTITLALLAYLKAHALRVRSFKVGPDYIDPMFHAAVTGQGCVNLDLFLTDEPFVRATYHHHCRGQQ
AAVIEGVMGLFDGRAGQGDFASTAHVARLLRLPVILVIDGSGAGFSVAATLYGFRRFDPRVRIAGVILNRVGSERHAQIL
SEAVRSQGLELLGTVYKDETIALPHRHLGLVPVEELSDFRRTQQQLAALAERSFDWQKLLPLVAIQPTAPAPARWEAEPL
PPVRIAVARDRAFSFYYQDNLELLAALGAQLETFSPLAGEWPDCRGYLLGGGFPELCAGELSAKTRFWEGLRGAVARGVP
LYAECGGLMVLGEALRTPEGRSHPMAGILEASCWMGKRTVLGYRVATALHSSCAVEAGRQLRGHLFHRSRMEPQPGAPLW
QLDDDEQEGWVRGNLHASYLHLHWGTQAWAARRFVRRCLAVAFEGKSTPARF
>Mature_451_residues
AIIIAAPASGTGKTTITLALLAYLKAHALRVRSFKVGPDYIDPMFHAAVTGQGCVNLDLFLTDEPFVRATYHHHCRGQQA
AVIEGVMGLFDGRAGQGDFASTAHVARLLRLPVILVIDGSGAGFSVAATLYGFRRFDPRVRIAGVILNRVGSERHAQILS
EAVRSQGLELLGTVYKDETIALPHRHLGLVPVEELSDFRRTQQQLAALAERSFDWQKLLPLVAIQPTAPAPARWEAEPLP
PVRIAVARDRAFSFYYQDNLELLAALGAQLETFSPLAGEWPDCRGYLLGGGFPELCAGELSAKTRFWEGLRGAVARGVPL
YAECGGLMVLGEALRTPEGRSHPMAGILEASCWMGKRTVLGYRVATALHSSCAVEAGRQLRGHLFHRSRMEPQPGAPLWQ
LDDDEQEGWVRGNLHASYLHLHWGTQAWAARRFVRRCLAVAFEGKSTPARF

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBB_GLOVI (Q7NF32)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926641.1
- ProteinModelPortal:   Q7NF32
- GeneID:   2602099
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.3695
- HOGENOM:   HBG634910
- OMA:   VFKCGPD
- ProtClustDB:   PRK01077
- BioCyc:   GVIO251221:GLR3695-MONOMER
- HAMAP:   MF_00027
- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00379

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: NA

Molecular weight: Translated: 49511; Mature: 49379

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS51274 GATASE_COBBQ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: ACT_SITE 325-325 ACT_SITE 421-421

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIIIAAPASGTGKTTITLALLAYLKAHALRVRSFKVGPDYIDPMFHAAVTGQGCVNLDL
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHCCCCCEEEEE
FLTDEPFVRATYHHHCRGQQAAVIEGVMGLFDGRAGQGDFASTAHVARLLRLPVILVIDG
EECCCCCEEHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEEC
SGAGFSVAATLYGFRRFDPRVRIAGVILNRVGSERHAQILSEAVRSQGLELLGTVYKDET
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCCE
IALPHRHLGLVPVEELSDFRRTQQQLAALAERSFDWQKLLPLVAIQPTAPAPARWEAEPL
EECCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEECCCCCCCCCCCCCCC
PPVRIAVARDRAFSFYYQDNLELLAALGAQLETFSPLAGEWPDCRGYLLGGGFPELCAGE
CCEEEEEEECCEEEEEECCCHHHHHHHCCHHHHCCCCCCCCCCCCEEEECCCCHHHHHHH
LSAKTRFWEGLRGAVARGVPLYAECGGLMVLGEALRTPEGRSHPMAGILEASCWMGKRTV
HHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHCCCHHH
LGYRVATALHSSCAVEAGRQLRGHLFHRSRMEPQPGAPLWQLDDDEQEGWVRGNLHASYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCEEEEEE
HLHWGTQAWAARRFVRRCLAVAFEGKSTPARF
EEECCCHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AIIIAAPASGTGKTTITLALLAYLKAHALRVRSFKVGPDYIDPMFHAAVTGQGCVNLDL
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHCCCCCEEEEE
FLTDEPFVRATYHHHCRGQQAAVIEGVMGLFDGRAGQGDFASTAHVARLLRLPVILVIDG
EECCCCCEEHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEEC
SGAGFSVAATLYGFRRFDPRVRIAGVILNRVGSERHAQILSEAVRSQGLELLGTVYKDET
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCCE
IALPHRHLGLVPVEELSDFRRTQQQLAALAERSFDWQKLLPLVAIQPTAPAPARWEAEPL
EECCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEECCCCCCCCCCCCCCC
PPVRIAVARDRAFSFYYQDNLELLAALGAQLETFSPLAGEWPDCRGYLLGGGFPELCAGE
CCEEEEEEECCEEEEEECCCHHHHHHHCCHHHHCCCCCCCCCCCCEEEECCCCHHHHHHH
LSAKTRFWEGLRGAVARGVPLYAECGGLMVLGEALRTPEGRSHPMAGILEASCWMGKRTV
HHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHCCCHHH
LGYRVATALHSSCAVEAGRQLRGHLFHRSRMEPQPGAPLWQLDDDEQEGWVRGNLHASYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCEEEEEE
HLHWGTQAWAARRFVRRCLAVAFEGKSTPARF
EEECCCHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292