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The map label for this gene is tvaII [H]

Identifier: 37523215

GI number: 37523215

Start: 3844078

End: 3845520

Strand: Direct

Name: tvaII [H]

Synonym: glr3646

Alternate gene names: 37523215

Gene position: 3844078-3845520 (Clockwise)

Preceding gene: 37523212

Following gene: 37523221

Centisome position: 82.51

GC content: 60.08

Gene sequence:

>1443_bases
ATGCAGATTCACACACCGAACTGGGTAAAGCACGCTATCTTCTATCAGATCTTTCCGGACCGCTTCTCCCGCACCCAGCA
GTTAAAACAAAAGCTGTTGCAGGCGGTCTCCTTCGAGGATTGGGCAGCCGCCCCGACCCTCTACGGCTATAAAGGCGGCG
ATCTGTGGGGGGTAATCGAAAGACTCGATTACCTGTCGGATTTGGGCATCAACGCCATCTATTTCACTCCGATATTTCAA
TCGGCGAGTAACCACCGCTACCATACCCACGATTACTATAGAGTCGATCCGCTGCTGGGAGGAAACGAGGCGTTCAAGGC
ATTGCTCGATGCCGCCCACGCCCGCGACATCAAAGTGGTGCTCGACGGTGTATTTAACCACGCGAGCCGCGGTTTTTTCT
TCTTCCACGACATCCTTGAAAATGGTCAACTCTCCCCGTGGCTCGACTGGTTCAAGATCGAAGGCTGGCCCCTCTCGCCC
TACAATGTCGAGCAGCCCGCCAACTATGCCTGCTGGGACAACAACCGTGCCCTGCCCCAGTTCAACCATGCCAATCCGAT
GGTGCGCGAGTACATTATGGAGATTGCCGAATACTGGATCAAAGCGGGTATCGACGGCTGGCGCCTCGATGTGCCCGCCT
GTGTAGAGGCGGAAGGTTTCTGGCAGGAATTTCGCGAGCGCGTCAAGGCAATCAATCCTGAAGCCTACATCGTCGGCGAA
ATCTGGGAGGATGCCCGCCAGTGGCTCGACGGCACCCAGTTCGACGGGGTGATGAATTATCTGTTTGCCGCCCCGACCAT
CGCCTTTACGGCCGGGGATCGCGTCCAGGTGCAGTACGTGCAGGACCGTTCCTACAGGCCCTACCCGGCGCTGTTCGCCG
TTGAGTACAAAGAGGCAATCGACAAATTGTTGGGGCTGTACGACTGGGAGATCCAGCTGGCGCAACTCAACTTGCTCGAT
AGCCACGACACGGCCAGGGTACTGACCATCGCCGGGGGCGACCGCGCGAGTGTAGAACTGGCGACACTGCTGTTGCTCAC
CTTTCCCGGGGCGCCCAGCATCTACTACGGCGACGAGGTGGGCCTGCCCGGCGGCATCGACCCTGATTCGCGCCAGGGTT
TTCCTCCCGAATCGCAGTGGAACGGCGAACTGCTCGCGCTGCACCGCGACTTGATCGCCCTGCGCCACCGCCACGAAGCG
CTGCGCATCGGCAAATACAAAAGCGTCTATGCCTCCGGCCAGTGCTATGTCTTCGAGCGCCGCCTGGAGGCGGAAGTGCT
GTTGGTGGCCGTCAACGCCGGGACCGAACCGGCGCGCGTCACCCTCGAAGCGCCTGGGGAAACCTACCCGCGCAAAGTGC
TCTTCGGTCCCGGCCAGGTGGAAGCCGCCGAAGGGCATCTGGAATTGAGCCTGCCGGCCCGCAGCGGTCTGGTGCTGGGG
TAG

Upstream 100 bases:

>100_bases
TGGTGGGGCAAACCGGCTTGCGATTCATCGCAAGGGGTCCAAAGTGCTTGCAAGTCTGTCCAATTCTGAGAGTCAAGCGA
GATTTCAAGGAAACACACCT

Downstream 100 bases:

>100_bases
ATAGACGTACTACGCCGCTTCTTCGGATTCGGCGGCGAGGGCTTCTTCCTCCGCCAGTCGGGCCGCCTCTTCTTCGGCTG
CGATGCGCGCTTCTTCTTCG

Product: neopullulanase

Products: NA

Alternate protein names: Alpha-amylase II; TVA II [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MQIHTPNWVKHAIFYQIFPDRFSRTQQLKQKLLQAVSFEDWAAAPTLYGYKGGDLWGVIERLDYLSDLGINAIYFTPIFQ
SASNHRYHTHDYYRVDPLLGGNEAFKALLDAAHARDIKVVLDGVFNHASRGFFFFHDILENGQLSPWLDWFKIEGWPLSP
YNVEQPANYACWDNNRALPQFNHANPMVREYIMEIAEYWIKAGIDGWRLDVPACVEAEGFWQEFRERVKAINPEAYIVGE
IWEDARQWLDGTQFDGVMNYLFAAPTIAFTAGDRVQVQYVQDRSYRPYPALFAVEYKEAIDKLLGLYDWEIQLAQLNLLD
SHDTARVLTIAGGDRASVELATLLLLTFPGAPSIYYGDEVGLPGGIDPDSRQGFPPESQWNGELLALHRDLIALRHRHEA
LRIGKYKSVYASGQCYVFERRLEAEVLLVAVNAGTEPARVTLEAPGETYPRKVLFGPGQVEAAEGHLELSLPARSGLVLG

Sequences:

>Translated_480_residues
MQIHTPNWVKHAIFYQIFPDRFSRTQQLKQKLLQAVSFEDWAAAPTLYGYKGGDLWGVIERLDYLSDLGINAIYFTPIFQ
SASNHRYHTHDYYRVDPLLGGNEAFKALLDAAHARDIKVVLDGVFNHASRGFFFFHDILENGQLSPWLDWFKIEGWPLSP
YNVEQPANYACWDNNRALPQFNHANPMVREYIMEIAEYWIKAGIDGWRLDVPACVEAEGFWQEFRERVKAINPEAYIVGE
IWEDARQWLDGTQFDGVMNYLFAAPTIAFTAGDRVQVQYVQDRSYRPYPALFAVEYKEAIDKLLGLYDWEIQLAQLNLLD
SHDTARVLTIAGGDRASVELATLLLLTFPGAPSIYYGDEVGLPGGIDPDSRQGFPPESQWNGELLALHRDLIALRHRHEA
LRIGKYKSVYASGQCYVFERRLEAEVLLVAVNAGTEPARVTLEAPGETYPRKVLFGPGQVEAAEGHLELSLPARSGLVLG
>Mature_480_residues
MQIHTPNWVKHAIFYQIFPDRFSRTQQLKQKLLQAVSFEDWAAAPTLYGYKGGDLWGVIERLDYLSDLGINAIYFTPIFQ
SASNHRYHTHDYYRVDPLLGGNEAFKALLDAAHARDIKVVLDGVFNHASRGFFFFHDILENGQLSPWLDWFKIEGWPLSP
YNVEQPANYACWDNNRALPQFNHANPMVREYIMEIAEYWIKAGIDGWRLDVPACVEAEGFWQEFRERVKAINPEAYIVGE
IWEDARQWLDGTQFDGVMNYLFAAPTIAFTAGDRVQVQYVQDRSYRPYPALFAVEYKEAIDKLLGLYDWEIQLAQLNLLD
SHDTARVLTIAGGDRASVELATLLLLTFPGAPSIYYGDEVGLPGGIDPDSRQGFPPESQWNGELLALHRDLIALRHRHEA
LRIGKYKSVYASGQCYVFERRLEAEVLLVAVNAGTEPARVTLEAPGETYPRKVLFGPGQVEAAEGHLELSLPARSGLVLG

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=354, Percent_Identity=25.4237288135593, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI1786604, Length=515, Percent_Identity=36.3106796116505, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1790687, Length=231, Percent_Identity=29.4372294372294, Blast_Score=96, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321726, Length=421, Percent_Identity=25.1781472684086, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322245, Length=231, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321731, Length=406, Percent_Identity=23.8916256157635, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319776, Length=406, Percent_Identity=23.8916256157635, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322241, Length=228, Percent_Identity=27.6315789473684, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6322021, Length=228, Percent_Identity=27.6315789473684, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324416, Length=228, Percent_Identity=27.6315789473684, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24586587, Length=430, Percent_Identity=25.1162790697674, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24583749, Length=405, Percent_Identity=26.9135802469136, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24583747, Length=405, Percent_Identity=26.9135802469136, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24583745, Length=407, Percent_Identity=24.8157248157248, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24586589, Length=411, Percent_Identity=25.5474452554745, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24586591, Length=225, Percent_Identity=28, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24586597, Length=412, Percent_Identity=25, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24586599, Length=229, Percent_Identity=28.3842794759825, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24586593, Length=231, Percent_Identity=28.1385281385281, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221330053, Length=236, Percent_Identity=26.6949152542373, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI45549022, Length=379, Percent_Identity=25.5936675461741, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 54503; Mature: 54503

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIHTPNWVKHAIFYQIFPDRFSRTQQLKQKLLQAVSFEDWAAAPTLYGYKGGDLWGVIE
CCCCCCCHHHHHHHEEECCHHHHHHHHHHHHHHHHHCCCHHCCCCCEECCCCCCHHHHHH
RLDYLSDLGINAIYFTPIFQSASNHRYHTHDYYRVDPLLGGNEAFKALLDAAHARDIKVV
HHHHHHHCCCCEEEEEEHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCHHHH
LDGVFNHASRGFFFFHDILENGQLSPWLDWFKIEGWPLSPYNVEQPANYACWDNNRALPQ
HHHHHHHCCCCEEEEHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCC
FNHANPMVREYIMEIAEYWIKAGIDGWRLDVPACVEAEGFWQEFRERVKAINPEAYIVGE
CCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEHH
IWEDARQWLDGTQFDGVMNYLFAAPTIAFTAGDRVQVQYVQDRSYRPYPALFAVEYKEAI
HHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCEEEEEEEECCCCCCCCCEEEEHHHHHH
DKLLGLYDWEIQLAQLNLLDSHDTARVLTIAGGDRASVELATLLLLTFPGAPSIYYGDEV
HHHHCCCCCEEEEEEEEEECCCCCEEEEEEECCCCCHHHHEEEEEEECCCCCCEEECCCC
GLPGGIDPDSRQGFPPESQWNGELLALHRDLIALRHRHEALRIGKYKSVYASGQCYVFER
CCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEC
RLEAEVLLVAVNAGTEPARVTLEAPGETYPRKVLFGPGQVEAAEGHLELSLPARSGLVLG
CCCEEEEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCEEECCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MQIHTPNWVKHAIFYQIFPDRFSRTQQLKQKLLQAVSFEDWAAAPTLYGYKGGDLWGVIE
CCCCCCCHHHHHHHEEECCHHHHHHHHHHHHHHHHHCCCHHCCCCCEECCCCCCHHHHHH
RLDYLSDLGINAIYFTPIFQSASNHRYHTHDYYRVDPLLGGNEAFKALLDAAHARDIKVV
HHHHHHHCCCCEEEEEEHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCHHHH
LDGVFNHASRGFFFFHDILENGQLSPWLDWFKIEGWPLSPYNVEQPANYACWDNNRALPQ
HHHHHHHCCCCEEEEHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCC
FNHANPMVREYIMEIAEYWIKAGIDGWRLDVPACVEAEGFWQEFRERVKAINPEAYIVGE
CCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEHH
IWEDARQWLDGTQFDGVMNYLFAAPTIAFTAGDRVQVQYVQDRSYRPYPALFAVEYKEAI
HHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCEEEEEEEECCCCCCCCCEEEEHHHHHH
DKLLGLYDWEIQLAQLNLLDSHDTARVLTIAGGDRASVELATLLLLTFPGAPSIYYGDEV
HHHHCCCCCEEEEEEEEEECCCCCEEEEEEECCCCCHHHHEEEEEEECCCCCCEEECCCC
GLPGGIDPDSRQGFPPESQWNGELLALHRDLIALRHRHEALRIGKYKSVYASGQCYVFER
CCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEC
RLEAEVLLVAVNAGTEPARVTLEAPGETYPRKVLFGPGQVEAAEGHLELSLPARSGLVLG
CCCEEEEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCEEECCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7763540; 10222200; 12051850 [H]