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The map label for this gene is trmFO [H]

Identifier: 37523172

GI number: 37523172

Start: 3798239

End: 3799711

Strand: Direct

Name: trmFO [H]

Synonym: glr3603

Alternate gene names: 37523172

Gene position: 3798239-3799711 (Clockwise)

Preceding gene: 37523169

Following gene: 37523173

Centisome position: 81.52

GC content: 65.99

Gene sequence:

>1473_bases
ATGGTCAGACTAAGTTTATTGATAGTCTTTGTCAACAACTCCGGGAAAATTGTCGCGGCGGCCGGTTTGCGGCTATGGGC
GGCGGGGGGATGGCACACTGGAGTGGTCGTCGAAACGTTGGCCATGATCGAGCCGGTGAGAGTGATTGGGGGCGGTATTG
CCGGGGCGGAGGCGGCCTGGCAGTGTGCGCGCGCCGGGGTGCCGGTGGTGCTCTCGGAGATGCGGCCGGTGCGACCCAGT
CCCGCCCACCACACCGACCACCTCGGAGAACTGGTGTGCAGCAATTCCTTCGGGGCGATGGCCACCGACCGGGCGCCGGG
ATTGCTCAAAGAAGAACTGCGGCGGTTGGGATCGCTGGTGATCGCCGTGGCCGACGCCCATGCCGTCCCGGCGGGGGGGG
CGCTGGCGGTGGACCGGGCGGTCTATACCCGGGAACTCACCGAGCGTGTGGCCAACCATCCGCTCATCGAATTGCGCCGC
GAGGAGGTGACGGAAATTCCCGCCGCGGGGCTCGTCGTCTTCGCCACCGGCCCGCTCACCTCCGAGCCGCTCGCCCACGG
TCTGCAGGCGCTCACGGGATTGGGGTACTTGAGCTTTTTCGACGCCGCAAGCCCGATCGTCGCGGGCGAATCGTTGGACA
CCGAAAAAGTCTTTCTGGCCTCGCGCTACGACCGGGGAGAGGCGGCTTATTACAATTGTCCGATGAGCGAGCCCGAGTAC
CGGGCGTTCTGGGAAGCGTTGGCCTCGGCCGAGCAGGCTGAACTCAAAGAATTCGAGCACGAGGAGCGCAAATTTTTTGA
AGCTTGCCTGCCGGTGGAAGAACTGGCCCGCCGCGGTTACGAGACGCTCCGCTACGGCCCGCTCAAGCCGGTGGGACTCA
CCGACCCGCGCACCGGCCGCTGGCCCTTTGCCTGTGTGCAGCTCAGGCAGGAGGACAGGGCGGGTCGCCTCTGGAATCTG
GTCGGCTTTCAGACCAACCTCAAATGGGGCGAGCAGAAGCGCGTCTTTCAGATGATCCCCGGTCTGGAGCAGGCCGAGTT
CGTCCGCCTTGGGGTGATGCACCGCAACACATTTTTGTGCTCGCCCCATCTGCTTGCCCCGACGCTGCAGTTTCACGCCC
ATCCCCGGCTACTGGCCTGCGGCCAGCTCACCGGCACCGAGGGCTACACGGCGGCGGTGGCGGGCGGTTGGCTCGCGGGG
ACCAACGCCGCCCGCCTGGCTCAGAGTAGAGATCCGCTGGTCTTGCCGGTCGAGACGATGGCGGGGGCGCTGGTGGATTA
CGTGAGCCACGGCGATCCGAAAACCTTCCAGCCGATGCCCCCCAATTTCGCCCTGCTTCCCGAAATTACCCCCCGCATCC
GCCACAAGCAGCAGCGCTACTGGGCCTACCGCGACCGCGCCCTGGCTGCAATTGACACCTTCGGCCGCACCCACGGGCTT
GGCGCCGCCCCTGCGCTTGCGAGGGCCAACTGA

Upstream 100 bases:

>100_bases
GGGAAAACCCAAAGCAATTGCCCCCGCAACGACCAACCCTATGGACAGGCTGGCCACCGCCGCTTTGCCCGCCCGGAAGG
CTGCTGCGAAAGCCGCCTTA

Downstream 100 bases:

>100_bases
TGTTCACCGGCATCGTCGAGGAGACGGGCACTCTGCTGGCTCTGGGCAGTGAAGAAATCGCCGTTCGGGCTGCGAAAGTG
CTCAAAGACGTGGCCCTGGG

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MVRLSLLIVFVNNSGKIVAAAGLRLWAAGGWHTGVVVETLAMIEPVRVIGGGIAGAEAAWQCARAGVPVVLSEMRPVRPS
PAHHTDHLGELVCSNSFGAMATDRAPGLLKEELRRLGSLVIAVADAHAVPAGGALAVDRAVYTRELTERVANHPLIELRR
EEVTEIPAAGLVVFATGPLTSEPLAHGLQALTGLGYLSFFDAASPIVAGESLDTEKVFLASRYDRGEAAYYNCPMSEPEY
RAFWEALASAEQAELKEFEHEERKFFEACLPVEELARRGYETLRYGPLKPVGLTDPRTGRWPFACVQLRQEDRAGRLWNL
VGFQTNLKWGEQKRVFQMIPGLEQAEFVRLGVMHRNTFLCSPHLLAPTLQFHAHPRLLACGQLTGTEGYTAAVAGGWLAG
TNAARLAQSRDPLVLPVETMAGALVDYVSHGDPKTFQPMPPNFALLPEITPRIRHKQQRYWAYRDRALAAIDTFGRTHGL
GAAPALARAN

Sequences:

>Translated_490_residues
MVRLSLLIVFVNNSGKIVAAAGLRLWAAGGWHTGVVVETLAMIEPVRVIGGGIAGAEAAWQCARAGVPVVLSEMRPVRPS
PAHHTDHLGELVCSNSFGAMATDRAPGLLKEELRRLGSLVIAVADAHAVPAGGALAVDRAVYTRELTERVANHPLIELRR
EEVTEIPAAGLVVFATGPLTSEPLAHGLQALTGLGYLSFFDAASPIVAGESLDTEKVFLASRYDRGEAAYYNCPMSEPEY
RAFWEALASAEQAELKEFEHEERKFFEACLPVEELARRGYETLRYGPLKPVGLTDPRTGRWPFACVQLRQEDRAGRLWNL
VGFQTNLKWGEQKRVFQMIPGLEQAEFVRLGVMHRNTFLCSPHLLAPTLQFHAHPRLLACGQLTGTEGYTAAVAGGWLAG
TNAARLAQSRDPLVLPVETMAGALVDYVSHGDPKTFQPMPPNFALLPEITPRIRHKQQRYWAYRDRALAAIDTFGRTHGL
GAAPALARAN
>Mature_490_residues
MVRLSLLIVFVNNSGKIVAAAGLRLWAAGGWHTGVVVETLAMIEPVRVIGGGIAGAEAAWQCARAGVPVVLSEMRPVRPS
PAHHTDHLGELVCSNSFGAMATDRAPGLLKEELRRLGSLVIAVADAHAVPAGGALAVDRAVYTRELTERVANHPLIELRR
EEVTEIPAAGLVVFATGPLTSEPLAHGLQALTGLGYLSFFDAASPIVAGESLDTEKVFLASRYDRGEAAYYNCPMSEPEY
RAFWEALASAEQAELKEFEHEERKFFEACLPVEELARRGYETLRYGPLKPVGLTDPRTGRWPFACVQLRQEDRAGRLWNL
VGFQTNLKWGEQKRVFQMIPGLEQAEFVRLGVMHRNTFLCSPHLLAPTLQFHAHPRLLACGQLTGTEGYTAAVAGGWLAG
TNAARLAQSRDPLVLPVETMAGALVDYVSHGDPKTFQPMPPNFALLPEITPRIRHKQQRYWAYRDRALAAIDTFGRTHGL
GAAPALARAN

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 53440; Mature: 53440

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRLSLLIVFVNNSGKIVAAAGLRLWAAGGWHTGVVVETLAMIEPVRVIGGGIAGAEAAW
CEEEEEEEEEECCCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHH
QCARAGVPVVLSEMRPVRPSPAHHTDHLGELVCSNSFGAMATDRAPGLLKEELRRLGSLV
HHHHCCCCEEHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHEE
IAVADAHAVPAGGALAVDRAVYTRELTERVANHPLIELRREEVTEIPAAGLVVFATGPLT
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCC
SEPLAHGLQALTGLGYLSFFDAASPIVAGESLDTEKVFLASRYDRGEAAYYNCPMSEPEY
CHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCEEEEECCCCCCHH
RAFWEALASAEQAELKEFEHEERKFFEACLPVEELARRGYETLRYGPLKPVGLTDPRTGR
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHCCCCCCCCCCCCCCCCC
WPFACVQLRQEDRAGRLWNLVGFQTNLKWGEQKRVFQMIPGLEQAEFVRLGVMHRNTFLC
CCHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHEECCEEEE
SPHLLAPTLQFHAHPRLLACGQLTGTEGYTAAVAGGWLAGTNAARLAQSRDPLVLPVETM
CCHHHCCHHHHCCCCCEEEECCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCEEEEHHHH
AGALVDYVSHGDPKTFQPMPPNFALLPEITPRIRHKQQRYWAYRDRALAAIDTFGRTHGL
HHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GAAPALARAN
CCCCCCCCCC
>Mature Secondary Structure
MVRLSLLIVFVNNSGKIVAAAGLRLWAAGGWHTGVVVETLAMIEPVRVIGGGIAGAEAAW
CEEEEEEEEEECCCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHH
QCARAGVPVVLSEMRPVRPSPAHHTDHLGELVCSNSFGAMATDRAPGLLKEELRRLGSLV
HHHHCCCCEEHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHEE
IAVADAHAVPAGGALAVDRAVYTRELTERVANHPLIELRREEVTEIPAAGLVVFATGPLT
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCC
SEPLAHGLQALTGLGYLSFFDAASPIVAGESLDTEKVFLASRYDRGEAAYYNCPMSEPEY
CHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCEEEEECCCCCCHH
RAFWEALASAEQAELKEFEHEERKFFEACLPVEELARRGYETLRYGPLKPVGLTDPRTGR
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHCCCCCCCCCCCCCCCCC
WPFACVQLRQEDRAGRLWNLVGFQTNLKWGEQKRVFQMIPGLEQAEFVRLGVMHRNTFLC
CCHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHEECCEEEE
SPHLLAPTLQFHAHPRLLACGQLTGTEGYTAAVAGGWLAGTNAARLAQSRDPLVLPVETM
CCHHHCCHHHHCCCCCEEEECCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCEEEEHHHH
AGALVDYVSHGDPKTFQPMPPNFALLPEITPRIRHKQQRYWAYRDRALAAIDTFGRTHGL
HHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GAAPALARAN
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]