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The map label for this gene is queA

Identifier: 37523156

GI number: 37523156

Start: 3777519

End: 3778577

Strand: Direct

Name: queA

Synonym: glr3587

Alternate gene names: 37523156

Gene position: 3777519-3778577 (Clockwise)

Preceding gene: 37523155

Following gene: 37523157

Centisome position: 81.08

GC content: 69.41

Gene sequence:

>1059_bases
ATGAACGCGCCCATTGATGAACGCGACTTTTTGACCGATAGCTACGACTACGCGCTGCCGGAGCGTTGCATCGCCCAGCA
ACCCGCCGAGCCGCGCGACCATTCGCGGCTATTGGTGGTGGACGGGGAGGCCCACACCCACCGCTACTTTTACGACCTGC
CGGGGTTGCTGCGGCCCGGAGATCTGCTGGTACTCAACGACACCCGCGTGATCCCCGCCCGCTTGTTCGGCAGCAAGGCG
AGCGGCGGTCGGGTGGAGGTGCTGCTGCTGGAGCCGCGCGCCCCCCGCGAATGGCTGTGCCTGGTCAAACCGGCCCGGCG
GCTCGCCGTCGGCGCCCGGATCGACTTTGACGGGGTGCTTGCCGCCCGGGTCACGGAGCTGGACGCCGAGACCGGCGGGC
GCTGGTTGCGCTTCGAGGGCGAGGAAGACTTCGAGGCGGCCCTCGAGCGCGTCGGTCACACGCCCCTGCCGCCTTACCTC
AAAACGGGCCGCACCCGCGACGAGCGCTATCAGACCCTCTGGGCGAGCCGGCCGGGGGCGGTGGCCGCCCCGACCGCGGG
CTTGCACTTCAGCGGGGAGTTGCTCGCCCGTCTGGCCGAGCGGGGCATCGAGCGCGCGACCGTCACCCTGCACGTGGGCC
TGGGCACCTTCCGCCCCGTACAGTCCGTTTCCGTCCATACCCATCGGATGCACCGCGAGTGGTACGAGATTCCCGAGGCG
ACCGCCATCGCCATCGAGCGCACCCGGTCGCGGGGCGGCCGGGTGCTGGCCGTGGGCACCACCAGTGCCCGTGCCCTCGA
AAGTGCGGCGCAACCGAACGGTTTGCCGGCCACCGGCCCGGGCCGCTCGGAGTTGTTTGTCTACCCGGGCTACCGCTGGC
GGGTGGTGGAGGGGCTGATCACCAACTTCCACCTGCCCAGGTCCAGTTTGCTGATGCTGGTCAGCAGCCTGGTGGGCCGG
GAGCGGCTGCTGGCCCTTTACCGGGAGGCCGTCGACAAAGGTTATCGCTTCTATTCGTTCGGGGATGCGATGTTGATTTT
GCCGGGCGCCGGGGCTTGA

Upstream 100 bases:

>100_bases
AGTTGCGCGCCCGCTTCGTGGAGCGCGGCGGTCGCGGCGGCGGCCGCGGCGGCGGTCCCCTGGGCCAACCCTGAAGGCCG
GGCGGGTTAAGATCCGTCCC

Downstream 100 bases:

>100_bases
GAGATTGACTTTCACCGACGGGCAAGAGCGCCCTGGTGAGCGGCAGCAGCGGCATCTGATATTTACTCGGCCGGAGAAGT
TGTGACTTGCTCGACAGGTA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MNAPIDERDFLTDSYDYALPERCIAQQPAEPRDHSRLLVVDGEAHTHRYFYDLPGLLRPGDLLVLNDTRVIPARLFGSKA
SGGRVEVLLLEPRAPREWLCLVKPARRLAVGARIDFDGVLAARVTELDAETGGRWLRFEGEEDFEAALERVGHTPLPPYL
KTGRTRDERYQTLWASRPGAVAAPTAGLHFSGELLARLAERGIERATVTLHVGLGTFRPVQSVSVHTHRMHREWYEIPEA
TAIAIERTRSRGGRVLAVGTTSARALESAAQPNGLPATGPGRSELFVYPGYRWRVVEGLITNFHLPRSSLLMLVSSLVGR
ERLLALYREAVDKGYRFYSFGDAMLILPGAGA

Sequences:

>Translated_352_residues
MNAPIDERDFLTDSYDYALPERCIAQQPAEPRDHSRLLVVDGEAHTHRYFYDLPGLLRPGDLLVLNDTRVIPARLFGSKA
SGGRVEVLLLEPRAPREWLCLVKPARRLAVGARIDFDGVLAARVTELDAETGGRWLRFEGEEDFEAALERVGHTPLPPYL
KTGRTRDERYQTLWASRPGAVAAPTAGLHFSGELLARLAERGIERATVTLHVGLGTFRPVQSVSVHTHRMHREWYEIPEA
TAIAIERTRSRGGRVLAVGTTSARALESAAQPNGLPATGPGRSELFVYPGYRWRVVEGLITNFHLPRSSLLMLVSSLVGR
ERLLALYREAVDKGYRFYSFGDAMLILPGAGA
>Mature_352_residues
MNAPIDERDFLTDSYDYALPERCIAQQPAEPRDHSRLLVVDGEAHTHRYFYDLPGLLRPGDLLVLNDTRVIPARLFGSKA
SGGRVEVLLLEPRAPREWLCLVKPARRLAVGARIDFDGVLAARVTELDAETGGRWLRFEGEEDFEAALERVGHTPLPPYL
KTGRTRDERYQTLWASRPGAVAAPTAGLHFSGELLARLAERGIERATVTLHVGLGTFRPVQSVSVHTHRMHREWYEIPEA
TAIAIERTRSRGGRVLAVGTTSARALESAAQPNGLPATGPGRSELFVYPGYRWRVVEGLITNFHLPRSSLLMLVSSLVGR
ERLLALYREAVDKGYRFYSFGDAMLILPGAGA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=44.6376811594203, Blast_Score=282, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_GLOVI (Q7NFD9)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926533.1
- ProteinModelPortal:   Q7NFD9
- SMR:   Q7NFD9
- GeneID:   2601991
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:glr3587
- NMPDR:   fig|251221.1.peg.3587
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- BioCyc:   GVIO251221:GLR3587-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39082; Mature: 39082

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPIDERDFLTDSYDYALPERCIAQQPAEPRDHSRLLVVDGEAHTHRYFYDLPGLLRPG
CCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCC
DLLVLNDTRVIPARLFGSKASGGRVEVLLLEPRAPREWLCLVKPARRLAVGARIDFDGVL
CEEEEECCEEEEHHHCCCCCCCCEEEEEEECCCCCHHHEEEECHHHHHEECCCCCCCCHH
AARVTELDAETGGRWLRFEGEEDFEAALERVGHTPLPPYLKTGRTRDERYQTLWASRPGA
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHCCCCCC
VAAPTAGLHFSGELLARLAERGIERATVTLHVGLGTFRPVQSVSVHTHRMHREWYEIPEA
CCCCCCCCEECHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
TAIAIERTRSRGGRVLAVGTTSARALESAAQPNGLPATGPGRSELFVYPGYRWRVVEGLI
EEEEEEHHHCCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEEHHHHH
TNFHLPRSSLLMLVSSLVGRERLLALYREAVDKGYRFYSFGDAMLILPGAGA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCC
>Mature Secondary Structure
MNAPIDERDFLTDSYDYALPERCIAQQPAEPRDHSRLLVVDGEAHTHRYFYDLPGLLRPG
CCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCC
DLLVLNDTRVIPARLFGSKASGGRVEVLLLEPRAPREWLCLVKPARRLAVGARIDFDGVL
CEEEEECCEEEEHHHCCCCCCCCEEEEEEECCCCCHHHEEEECHHHHHEECCCCCCCCHH
AARVTELDAETGGRWLRFEGEEDFEAALERVGHTPLPPYLKTGRTRDERYQTLWASRPGA
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHCCCCCC
VAAPTAGLHFSGELLARLAERGIERATVTLHVGLGTFRPVQSVSVHTHRMHREWYEIPEA
CCCCCCCCEECHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
TAIAIERTRSRGGRVLAVGTTSARALESAAQPNGLPATGPGRSELFVYPGYRWRVVEGLI
EEEEEEHHHCCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEEHHHHH
TNFHLPRSSLLMLVSSLVGRERLLALYREAVDKGYRFYSFGDAMLILPGAGA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292