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The map label for this gene is truA

Identifier: 37523138

GI number: 37523138

Start: 3762239

End: 3763153

Strand: Reverse

Name: truA

Synonym: gll3569

Alternate gene names: 37523138

Gene position: 3763153-3762239 (Counterclockwise)

Preceding gene: 37523139

Following gene: 37523137

Centisome position: 80.77

GC content: 65.36

Gene sequence:

>915_bases
ATGTCCGTGGATGCGGTGGGCGGCGCCGTAGAAGGCGCACCGGCGCGCCTTGCCCTCCGGGTGCAGTACCTGGGGGGACA
TTTTTTTGGCTGGCAATGGCAGCCGGGTCAGCGCACAGTGCAGCAGTGTTTGGAGGAAGCGACCGAGCGCATCGCCCGCC
ATCCGGTGCGCTACCACGCGGCGGGGCGCACCGACACCGGTGTGCACGCGAGCGGCCAGGTCGTCCACTTTGATACCCAC
AAAAAGCTGGCCCCCGAGACCTGGGTGCGCGGTCTCAACAGCTTGCTTCCCGACGACATCGCCGTGCGCGCGGCGGCCTA
CGTGAGCGACCACTGGCACGCGCGCTTCACCGCGCTGTGGCGTGAGTACCGCTACTGCATCCACAACAGCACCGTGCCCG
ACCTATTCGTGCGCGCCCAGAGCTGGTACTACCCCTATTGCCCCCTCGACAGCGAAGCGGTGGCGGCAGCCCTGGCCACT
TTGCCGGGCCACCACGATTTTCGGGCGTTTCGCCGCGCCGGCTCCAGCCGCCCCCACTCCCTGGTCCATGTCTATACAGC
CGAGTGTACCCGCGAGGGGGAGCAAATCGCGATTCGGGTGCGGGCCAACAGTTTTCTGTACGGGATGATGCGCCTGTTGA
TTGGAGCACTGGCCGAGGTGGGGTCGGGGCGCTGGAGTGTGGAGCGCTTCGCCGGTCTGTGGCAGGCCGGCAACCGCGAA
CAAGTCAAGTACGCCGCCCCACCCCAGGGACTGTGTCTGGTGGGGGTGGGCTACCCGGACGATCCGTTCAAGACGAACGG
AATCGGTTTTGCCCGATGTCATACTGGGCAGGAAAAGCCAGAAGCTAGACTGGGAAATGGCGATTTGGAATCTCGAGAGG
AGCGACCGCCCCATGAAATGTCCCCGCTGCACTGA

Upstream 100 bases:

>100_bases
AGGAGCGCTACGGTAGCCGCCGCGGCGGTTACACCCGCATCCTGCGCACGGTGGCCCGCCGCGGCGACAACTCCCCGATG
GCGATTATCGAACTGGTCTG

Downstream 100 bases:

>100_bases
CAATGAACTGAAGACGGTCAAATCCGACGACATCGAAGTCGATCAGTGCCCCAACTGCCTCGGTATCTGGTTCGATGTGA
GCGAAATGACCGCCCTGGTA

Product: tRNA-pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSVDAVGGAVEGAPARLALRVQYLGGHFFGWQWQPGQRTVQQCLEEATERIARHPVRYHAAGRTDTGVHASGQVVHFDTH
KKLAPETWVRGLNSLLPDDIAVRAAAYVSDHWHARFTALWREYRYCIHNSTVPDLFVRAQSWYYPYCPLDSEAVAAALAT
LPGHHDFRAFRRAGSSRPHSLVHVYTAECTREGEQIAIRVRANSFLYGMMRLLIGALAEVGSGRWSVERFAGLWQAGNRE
QVKYAAPPQGLCLVGVGYPDDPFKTNGIGFARCHTGQEKPEARLGNGDLESREERPPHEMSPLH

Sequences:

>Translated_304_residues
MSVDAVGGAVEGAPARLALRVQYLGGHFFGWQWQPGQRTVQQCLEEATERIARHPVRYHAAGRTDTGVHASGQVVHFDTH
KKLAPETWVRGLNSLLPDDIAVRAAAYVSDHWHARFTALWREYRYCIHNSTVPDLFVRAQSWYYPYCPLDSEAVAAALAT
LPGHHDFRAFRRAGSSRPHSLVHVYTAECTREGEQIAIRVRANSFLYGMMRLLIGALAEVGSGRWSVERFAGLWQAGNRE
QVKYAAPPQGLCLVGVGYPDDPFKTNGIGFARCHTGQEKPEARLGNGDLESREERPPHEMSPLH
>Mature_303_residues
SVDAVGGAVEGAPARLALRVQYLGGHFFGWQWQPGQRTVQQCLEEATERIARHPVRYHAAGRTDTGVHASGQVVHFDTHK
KLAPETWVRGLNSLLPDDIAVRAAAYVSDHWHARFTALWREYRYCIHNSTVPDLFVRAQSWYYPYCPLDSEAVAAALATL
PGHHDFRAFRRAGSSRPHSLVHVYTAECTREGEQIAIRVRANSFLYGMMRLLIGALAEVGSGRWSVERFAGLWQAGNREQ
VKYAAPPQGLCLVGVGYPDDPFKTNGIGFARCHTGQEKPEARLGNGDLESREERPPHEMSPLH

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=290, Percent_Identity=31.7241379310345, Blast_Score=88, Evalue=9e-18,
Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=38.2113821138211, Blast_Score=166, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_GLOVI (Q7MBB9)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926515.1
- ProteinModelPortal:   Q7MBB9
- SMR:   Q7MBB9
- GeneID:   2601973
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll3569
- NMPDR:   fig|251221.1.peg.3569
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- BioCyc:   GVIO251221:GLL3569-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 33935; Mature: 33803

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: ACT_SITE 65-65

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDAVGGAVEGAPARLALRVQYLGGHFFGWQWQPGQRTVQQCLEEATERIARHPVRYHA
CCCCCCCCCCCCCCCEEEEEEEECCCEEECEEECCCHHHHHHHHHHHHHHHHHCCEEEEE
AGRTDTGVHASGQVVHFDTHKKLAPETWVRGLNSLLPDDIAVRAAAYVSDHWHARFTALW
CCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
REYRYCIHNSTVPDLFVRAQSWYYPYCPLDSEAVAAALATLPGHHDFRAFRRAGSSRPHS
HHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCE
LVHVYTAECTREGEQIAIRVRANSFLYGMMRLLIGALAEVGSGRWSVERFAGLWQAGNRE
EEEEEEHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
QVKYAAPPQGLCLVGVGYPDDPFKTNGIGFARCHTGQEKPEARLGNGDLESREERPPHEM
CEEECCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
SPLH
CCCC
>Mature Secondary Structure 
SVDAVGGAVEGAPARLALRVQYLGGHFFGWQWQPGQRTVQQCLEEATERIARHPVRYHA
CCCCCCCCCCCCCCEEEEEEEECCCEEECEEECCCHHHHHHHHHHHHHHHHHCCEEEEE
AGRTDTGVHASGQVVHFDTHKKLAPETWVRGLNSLLPDDIAVRAAAYVSDHWHARFTALW
CCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
REYRYCIHNSTVPDLFVRAQSWYYPYCPLDSEAVAAALATLPGHHDFRAFRRAGSSRPHS
HHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCE
LVHVYTAECTREGEQIAIRVRANSFLYGMMRLLIGALAEVGSGRWSVERFAGLWQAGNRE
EEEEEEHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
QVKYAAPPQGLCLVGVGYPDDPFKTNGIGFARCHTGQEKPEARLGNGDLESREERPPHEM
CEEECCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
SPLH
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292