Click here to switch to the map view.

The map label for this gene is pyk2 [H]

Identifier: 37523064

GI number: 37523064

Start: 3704206

End: 3706023

Strand: Reverse

Name: pyk2 [H]

Synonym: gll3495

Alternate gene names: 37523064

Gene position: 3706023-3704206 (Counterclockwise)

Preceding gene: 37523065

Following gene: 37523063

Centisome position: 79.55

GC content: 64.52

Gene sequence:

>1818_bases
ATGCGCGTGGGTGCCGATGTGTTCGTTCTAGATGCCAATGCCCAACAGACAACTGCCGCGCCGAGCGAGCGGGCGCGTTT
TCGCCGCACCAAGATTGTCGCCACCATCGGTCCCGCCGTCGACGACCCGCAGGCCCTGCGCAAGCTCATCCAGGCCGGGG
CGACGACCCTCAGACTCAATTTTTCCCACGGCAACCACGAGGAGCACCGCCGCCGCATCGGCCTCATCCGCCAGATTTCC
TTTGAACTCAATCAGCCGGTGGCCATCCTGCAGGATCTGCAAGGTCCCAAGATCCGGTTGGGCAAGTTCGCCGACGGACC
AATCGTCCTCAAGCACGGCGACCCGTTTATTCTGACCAGCCACGACGTGCCCGCCAACCAGCACATCAGTCCGATCACCT
ACTCGCGACTTATCCAGGAAGTGCCCACCGGCGCCACGATCTTGCTGGATGACGGGCGGGTGGAACTGGTAGTCGAACGG
GTGGACCTCGAAGCCCAATACCTGCACTGCCGGACGGTGGGGGGGGGCAAGCTCTCCAACAGCAAAGGCGTCAACTTTCC
GGGTGTCACCCTCTCGGTGCCGGCGATGACCGAAAAGGACCGCGAGGATTTGCTCTTCGGTCTCAATCAGGGCGTCGATT
GGGTGGCCCTCAGTTTCGTGCGCACCCCGAGCGATTTGCTCGAACTGAAGGAACTGATTGCCCAATCGGGCAAGCGGGTG
CCGGTGATCGCCAAGATCGAAAAATTCGAGGCCTTCGAGAACCTCGATGCGGTGATTGCCGCCTGCGACGGGCTGATGGT
GGCCCGTGGCGATCTGGGCGTCGAGATGCCCGCCGAGGAAGTGCCCTTGCTCCAAAAGCGGCTGATCGCTTCGGCCAATC
GGGCCGGCAAGCCTGTGATCACCGCCACCCAGATGCTCGATTCGATGGTGAGCAACAGCCGCCCGACCCGCGCCGAAGTT
TCCGATGTGGCCAACGCCATCCTGGACGGCACCGACGCGGTGATGCTCTCCAACGAGACCGCCGTGGGCGAGCACCCGAT
CTTGGCGGTCGAGACGATGGCGCGCATCGCCGATCGCATCGAGCGCTCGATGCCCCAGCGCACCTGGGTAGACACGGGCC
GCTCGATCCCGAACGCCATCTCCCAGGCAGTGGGAAGCGTCGCCCAGCAATTGGGTGCCCAGGCGATTCTCACCCTCACC
AAGTCCGGCGCCACCGCCCGCAACGTCAGCAAGTACCGGCCGAGGGTGCCGATTATCGCGATTACCCCGAGCGTGTCGGT
GGCGCGCGAACTGCAGATGGTCTGGGGGGTCTACCCGCTGATGGTCCTGGATCTGCCTTCGACCCGCCAGACCTTTCAAG
CGGCGATCAGCGCCGCCCAGGACGAAAACCTCGTCCTGGAAGGCGACCTGGTGGTGATGACCGCCGGCACCATCCAGGGG
CTCTCCGGTTCGACGGACCTGATCAAAGTCGAGGTGGTGACGGCGATTCTCGGCCGGGGATTGGGCCTGGGTCAGAACGC
CGTGAGTGGACGCGCCCGCATCGTCCGCCACAGCGACGAAGCGGCGGCCTTGAGTCCCGGGGACATCCTGATTGCCCCGC
GCACCGACGCCAATTTTATCGAAGCGATCAAGCTCGCGGGCGGCATCGTCACCGAGGAGAGCGGCCTCACCTCCCACGCA
GCGATTATCGGGCTGCGCCTCGGACTGCCGGTACTGCTCGGGGTGAAAGACTGCACCACCCGCATCCCGGACGGGGTGGT
GCTCACCCTCGATCCGCAAAAAGGGGTCATCTTCTCCGGGGCGGCCTCCGCCGTCTAG

Upstream 100 bases:

>100_bases
CCTGGGTTACGACATCGACGCCGAATTTTTCGAGTTGCTCACCCTCTAGAGCCGACATGGCCATCGGTCTTAAGAAGCGC
ATGCTACGATTTGCGCAAAA

Downstream 100 bases:

>100_bases
CGCCTGTGGACAAAATTGTCGTTTTCTACTCCAAGCCCGGCTGCTGTCTGTGCGACGCGCTGGAAGCCACCTTGCGCCGG
GTGCAGCCGGATTTGGGCTT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MRVGADVFVLDANAQQTTAAPSERARFRRTKIVATIGPAVDDPQALRKLIQAGATTLRLNFSHGNHEEHRRRIGLIRQIS
FELNQPVAILQDLQGPKIRLGKFADGPIVLKHGDPFILTSHDVPANQHISPITYSRLIQEVPTGATILLDDGRVELVVER
VDLEAQYLHCRTVGGGKLSNSKGVNFPGVTLSVPAMTEKDREDLLFGLNQGVDWVALSFVRTPSDLLELKELIAQSGKRV
PVIAKIEKFEAFENLDAVIAACDGLMVARGDLGVEMPAEEVPLLQKRLIASANRAGKPVITATQMLDSMVSNSRPTRAEV
SDVANAILDGTDAVMLSNETAVGEHPILAVETMARIADRIERSMPQRTWVDTGRSIPNAISQAVGSVAQQLGAQAILTLT
KSGATARNVSKYRPRVPIIAITPSVSVARELQMVWGVYPLMVLDLPSTRQTFQAAISAAQDENLVLEGDLVVMTAGTIQG
LSGSTDLIKVEVVTAILGRGLGLGQNAVSGRARIVRHSDEAAALSPGDILIAPRTDANFIEAIKLAGGIVTEESGLTSHA
AIIGLRLGLPVLLGVKDCTTRIPDGVVLTLDPQKGVIFSGAASAV

Sequences:

>Translated_605_residues
MRVGADVFVLDANAQQTTAAPSERARFRRTKIVATIGPAVDDPQALRKLIQAGATTLRLNFSHGNHEEHRRRIGLIRQIS
FELNQPVAILQDLQGPKIRLGKFADGPIVLKHGDPFILTSHDVPANQHISPITYSRLIQEVPTGATILLDDGRVELVVER
VDLEAQYLHCRTVGGGKLSNSKGVNFPGVTLSVPAMTEKDREDLLFGLNQGVDWVALSFVRTPSDLLELKELIAQSGKRV
PVIAKIEKFEAFENLDAVIAACDGLMVARGDLGVEMPAEEVPLLQKRLIASANRAGKPVITATQMLDSMVSNSRPTRAEV
SDVANAILDGTDAVMLSNETAVGEHPILAVETMARIADRIERSMPQRTWVDTGRSIPNAISQAVGSVAQQLGAQAILTLT
KSGATARNVSKYRPRVPIIAITPSVSVARELQMVWGVYPLMVLDLPSTRQTFQAAISAAQDENLVLEGDLVVMTAGTIQG
LSGSTDLIKVEVVTAILGRGLGLGQNAVSGRARIVRHSDEAAALSPGDILIAPRTDANFIEAIKLAGGIVTEESGLTSHA
AIIGLRLGLPVLLGVKDCTTRIPDGVVLTLDPQKGVIFSGAASAV
>Mature_605_residues
MRVGADVFVLDANAQQTTAAPSERARFRRTKIVATIGPAVDDPQALRKLIQAGATTLRLNFSHGNHEEHRRRIGLIRQIS
FELNQPVAILQDLQGPKIRLGKFADGPIVLKHGDPFILTSHDVPANQHISPITYSRLIQEVPTGATILLDDGRVELVVER
VDLEAQYLHCRTVGGGKLSNSKGVNFPGVTLSVPAMTEKDREDLLFGLNQGVDWVALSFVRTPSDLLELKELIAQSGKRV
PVIAKIEKFEAFENLDAVIAACDGLMVARGDLGVEMPAEEVPLLQKRLIASANRAGKPVITATQMLDSMVSNSRPTRAEV
SDVANAILDGTDAVMLSNETAVGEHPILAVETMARIADRIERSMPQRTWVDTGRSIPNAISQAVGSVAQQLGAQAILTLT
KSGATARNVSKYRPRVPIIAITPSVSVARELQMVWGVYPLMVLDLPSTRQTFQAAISAAQDENLVLEGDLVVMTAGTIQG
LSGSTDLIKVEVVTAILGRGLGLGQNAVSGRARIVRHSDEAAALSPGDILIAPRTDANFIEAIKLAGGIVTEESGLTSHA
AIIGLRLGLPVLLGVKDCTTRIPDGVVLTLDPQKGVIFSGAASAV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=39.3075356415479, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=39.3075356415479, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI32967597, Length=495, Percent_Identity=39.1919191919192, Blast_Score=319, Evalue=4e-87,
Organism=Homo sapiens, GI10835121, Length=495, Percent_Identity=39.1919191919192, Blast_Score=319, Evalue=5e-87,
Organism=Homo sapiens, GI33286418, Length=491, Percent_Identity=39.3075356415479, Blast_Score=318, Evalue=7e-87,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=40.4844290657439, Blast_Score=205, Evalue=1e-52,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=40.4844290657439, Blast_Score=205, Evalue=1e-52,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=36.5957446808511, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=36.5957446808511, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=35.8139534883721, Blast_Score=129, Evalue=8e-30,
Organism=Escherichia coli, GI1787965, Length=473, Percent_Identity=42.4947145877378, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=39.9581589958159, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1787994, Length=100, Percent_Identity=34, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17544584, Length=504, Percent_Identity=39.8809523809524, Blast_Score=333, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17506829, Length=489, Percent_Identity=39.8773006134969, Blast_Score=304, Evalue=9e-83,
Organism=Caenorhabditis elegans, GI71984413, Length=489, Percent_Identity=39.8773006134969, Blast_Score=304, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI71984406, Length=489, Percent_Identity=39.8773006134969, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17506831, Length=489, Percent_Identity=39.8773006134969, Blast_Score=303, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17564524, Length=120, Percent_Identity=34.1666666666667, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319279, Length=488, Percent_Identity=40.7786885245902, Blast_Score=324, Evalue=3e-89,
Organism=Saccharomyces cerevisiae, GI6324923, Length=483, Percent_Identity=37.2670807453416, Blast_Score=288, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24648964, Length=497, Percent_Identity=39.6378269617706, Blast_Score=335, Evalue=6e-92,
Organism=Drosophila melanogaster, GI28571814, Length=497, Percent_Identity=39.6378269617706, Blast_Score=334, Evalue=8e-92,
Organism=Drosophila melanogaster, GI24648966, Length=414, Percent_Identity=39.8550724637681, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24581235, Length=485, Percent_Identity=33.6082474226804, Blast_Score=255, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24646914, Length=279, Percent_Identity=39.7849462365591, Blast_Score=196, Evalue=4e-50,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 64545; Mature: 64545

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGADVFVLDANAQQTTAAPSERARFRRTKIVATIGPAVDDPQALRKLIQAGATTLRLN
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCCEEEEEE
FSHGNHEEHRRRIGLIRQISFELNQPVAILQDLQGPKIRLGKFADGPIVLKHGDPFILTS
ECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEECEECCCCEEEECCCCEEEEC
HDVPANQHISPITYSRLIQEVPTGATILLDDGRVELVVERVDLEAQYLHCRTVGGGKLSN
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCEEEEEEEECCCEEEEEEEECCCCCCCC
SKGVNFPGVTLSVPAMTEKDREDLLFGLNQGVDWVALSFVRTPSDLLELKELIAQSGKRV
CCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCEEEHHHHCCCHHHHHHHHHHHCCCCCC
PVIAKIEKFEAFENLDAVIAACDGLMVARGDLGVEMPAEEVPLLQKRLIASANRAGKPVI
EEEEHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEE
TATQMLDSMVSNSRPTRAEVSDVANAILDGTDAVMLSNETAVGEHPILAVETMARIADRI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
ERSMPQRTWVDTGRSIPNAISQAVGSVAQQLGAQAILTLTKSGATARNVSKYRPRVPIIA
HHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCEEE
ITPSVSVARELQMVWGVYPLMVLDLPSTRQTFQAAISAAQDENLVLEGDLVVMTAGTIQG
ECCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHCCCCCEEEECCEEEEEECCCCC
LSGSTDLIKVEVVTAILGRGLGLGQNAVSGRARIVRHSDEAAALSPGDILIAPRTDANFI
CCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEECCCCEEEECCCCCHHH
EAIKLAGGIVTEESGLTSHAAIIGLRLGLPVLLGVKDCTTRIPDGVVLTLDPQKGVIFSG
HHHHHHCCEEECCCCCCHHHHHHHHHHCCHHEEECHHHHHCCCCCEEEEECCCCCEEECC
AASAV
CCCCC
>Mature Secondary Structure
MRVGADVFVLDANAQQTTAAPSERARFRRTKIVATIGPAVDDPQALRKLIQAGATTLRLN
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCCEEEEEE
FSHGNHEEHRRRIGLIRQISFELNQPVAILQDLQGPKIRLGKFADGPIVLKHGDPFILTS
ECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEECEECCCCEEEECCCCEEEEC
HDVPANQHISPITYSRLIQEVPTGATILLDDGRVELVVERVDLEAQYLHCRTVGGGKLSN
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCEEEEEEEECCCEEEEEEEECCCCCCCC
SKGVNFPGVTLSVPAMTEKDREDLLFGLNQGVDWVALSFVRTPSDLLELKELIAQSGKRV
CCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCEEEHHHHCCCHHHHHHHHHHHCCCCCC
PVIAKIEKFEAFENLDAVIAACDGLMVARGDLGVEMPAEEVPLLQKRLIASANRAGKPVI
EEEEHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEE
TATQMLDSMVSNSRPTRAEVSDVANAILDGTDAVMLSNETAVGEHPILAVETMARIADRI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
ERSMPQRTWVDTGRSIPNAISQAVGSVAQQLGAQAILTLTKSGATARNVSKYRPRVPIIA
HHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCEEE
ITPSVSVARELQMVWGVYPLMVLDLPSTRQTFQAAISAAQDENLVLEGDLVVMTAGTIQG
ECCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHCCCCCEEEECCEEEEEECCCCC
LSGSTDLIKVEVVTAILGRGLGLGQNAVSGRARIVRHSDEAAALSPGDILIAPRTDANFI
CCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEECCCCEEEECCCCCHHH
EAIKLAGGIVTEESGLTSHAAIIGLRLGLPVLLGVKDCTTRIPDGVVLTLDPQKGVIFSG
HHHHHHCCEEECCCCCCHHHHHHHHHHCCHHEEECHHHHHCCCCCEEEEECCCCCEEECC
AASAV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]