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The map label for this gene is leuC

Identifier: 37522986

GI number: 37522986

Start: 3614036

End: 3615457

Strand: Direct

Name: leuC

Synonym: gvip467

Alternate gene names: 37522986

Gene position: 3614036-3615457 (Clockwise)

Preceding gene: 37522980

Following gene: 37522987

Centisome position: 77.57

GC content: 64.7

Gene sequence:

>1422_bases
ATGGCCGTCAGCCGCGCAACGCTTTTTGACAAAGTCTGGGATCTGCACACGGTGGGCACTCTGGCCTCCGGTCAGACCCA
GCTTTTTATCGGACTGCACCTGATTCATGAGGTGACCAGTCCCCAGGCCTTTGCGATGTTGCGCGAGCGAGGTTTGGGGG
TGCTCTATGCGGAACGGACAGTGGCGACAGTAGACCACATCGTGCCCACCCAGAGCCAGCAGCGGCCTTTTGCCGACACG
CTGGCCGAGCAGATGATCCGCGCCCTCGAAGATAACTGTGCCGAATTCGGCATTCGCTTTTACAACATCGGTTCGGGTTC
CCAGGGCATCGTGCACGTCATCGCCCCCGAGCAGGGGTTCACCCAGCCGGGCATGACCATCGCCTGCGGCGACAGCCATA
CCGCCACCCACGGCGCTTTCGGCGCCATCGCCTTCGGCATCGGCACCAGCCAGGTGCGCGACGTGCTCGCCACCCAGACG
CTCGCCCTAGACAAGCTCAAAGTGCGCCGCATCGAGATGAACGGCAGCCTGCACCCGGGGGTGTTTGCCAAGGATGTGAT
TTTGCACATCATCCGCAAGCTCGGTGTCAAAGCCGGGGTGGGCTATGCCTACGAATTTGCCGGCACCACCTTCGAGGCGA
TGAATATGGAAGAGCGGATGACCGTCTGCAATATGGCCATCGAGGGCGGTGCCCGCTGCGGCTACATCAACCCCGACGCC
GTCACCTACGCCTACCTCAAAGGCCGCGACTTCGCCCCCCAGGGCGCCGACTGGGAGCGGGCGGTCGCCTGGTGGGAGAA
CCTGCGCTCCGACCCCGACGCCCAGTACGACGACACCATACACTTCGATGCCGCCGAGATTGCCCCCACCGTCACCTGGG
GCATCACCCCGGGTCAGGGGATTGCCGTCGACGAGATCGTTCCCCGGCCGGAGGAACTCCCCGGCGAAGATCGGCCTCTG
GCGGAAGAAGCTTATCGCTACATGGATCTGGCCCCAGGGGTGCCCATCGTCGGCACCAAGGTCGATGTCTGCTTCATCGG
CAGCTGCACCAACGGACGTCTGAGCGATCTGCGCGAAGCAGCCAATCTCGCCCGGGGCCGGCATGTCGCCCCGGGCGTCA
AAGCTTTCGTCGTCCCCGGCTCCGAGCGCGTCAAGCGGCAGGCTGAAGCCGAGGGTCTGCACGAAGTCTTTTTAGAAGCC
GGTTTCGAGTGGCGCGAGCCCGGCTGTTCGATGTGCCTGGCGATGAACCCCGACCGGCTCGAAGGCCGCCAACTGAGCGC
CTCCTCCTCCAACCGCAACTTCAAGGGTCGCCAGGGCTCAGCCTCCGGCCGCACGCTGCTGATGAGCCCGGCGATGGTGG
TGGCCGCTGCCGTGAGCGGTGCGGTCGCCGACGTTCGCGCCCTATTGGAGCCCACCCCATGA

Upstream 100 bases:

>100_bases
CCACAACCATAGCACACAAGTGTGCGGCGCCGCCCGTGTTTTTTGCTTTTTTCGGTTTTTGCACTTTTCGCTACAATAGC
GGGGCAGTATCGGGACTCCC

Downstream 100 bases:

>100_bases
GCCGCATCGTCAGTATCGAAGGCCGGGCCATGCCGCTACCGGGCCACGACATCGACACCGACCGGATCATCCCGGCGCGT
TTTTTGCGCTGCGTCACCTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MAVSRATLFDKVWDLHTVGTLASGQTQLFIGLHLIHEVTSPQAFAMLRERGLGVLYAERTVATVDHIVPTQSQQRPFADT
LAEQMIRALEDNCAEFGIRFYNIGSGSQGIVHVIAPEQGFTQPGMTIACGDSHTATHGAFGAIAFGIGTSQVRDVLATQT
LALDKLKVRRIEMNGSLHPGVFAKDVILHIIRKLGVKAGVGYAYEFAGTTFEAMNMEERMTVCNMAIEGGARCGYINPDA
VTYAYLKGRDFAPQGADWERAVAWWENLRSDPDAQYDDTIHFDAAEIAPTVTWGITPGQGIAVDEIVPRPEELPGEDRPL
AEEAYRYMDLAPGVPIVGTKVDVCFIGSCTNGRLSDLREAANLARGRHVAPGVKAFVVPGSERVKRQAEAEGLHEVFLEA
GFEWREPGCSMCLAMNPDRLEGRQLSASSSNRNFKGRQGSASGRTLLMSPAMVVAAAVSGAVADVRALLEPTP

Sequences:

>Translated_473_residues
MAVSRATLFDKVWDLHTVGTLASGQTQLFIGLHLIHEVTSPQAFAMLRERGLGVLYAERTVATVDHIVPTQSQQRPFADT
LAEQMIRALEDNCAEFGIRFYNIGSGSQGIVHVIAPEQGFTQPGMTIACGDSHTATHGAFGAIAFGIGTSQVRDVLATQT
LALDKLKVRRIEMNGSLHPGVFAKDVILHIIRKLGVKAGVGYAYEFAGTTFEAMNMEERMTVCNMAIEGGARCGYINPDA
VTYAYLKGRDFAPQGADWERAVAWWENLRSDPDAQYDDTIHFDAAEIAPTVTWGITPGQGIAVDEIVPRPEELPGEDRPL
AEEAYRYMDLAPGVPIVGTKVDVCFIGSCTNGRLSDLREAANLARGRHVAPGVKAFVVPGSERVKRQAEAEGLHEVFLEA
GFEWREPGCSMCLAMNPDRLEGRQLSASSSNRNFKGRQGSASGRTLLMSPAMVVAAAVSGAVADVRALLEPTP
>Mature_472_residues
AVSRATLFDKVWDLHTVGTLASGQTQLFIGLHLIHEVTSPQAFAMLRERGLGVLYAERTVATVDHIVPTQSQQRPFADTL
AEQMIRALEDNCAEFGIRFYNIGSGSQGIVHVIAPEQGFTQPGMTIACGDSHTATHGAFGAIAFGIGTSQVRDVLATQTL
ALDKLKVRRIEMNGSLHPGVFAKDVILHIIRKLGVKAGVGYAYEFAGTTFEAMNMEERMTVCNMAIEGGARCGYINPDAV
TYAYLKGRDFAPQGADWERAVAWWENLRSDPDAQYDDTIHFDAAEIAPTVTWGITPGQGIAVDEIVPRPEELPGEDRPLA
EEAYRYMDLAPGVPIVGTKVDVCFIGSCTNGRLSDLREAANLARGRHVAPGVKAFVVPGSERVKRQAEAEGLHEVFLEAG
FEWREPGCSMCLAMNPDRLEGRQLSASSSNRNFKGRQGSASGRTLLMSPAMVVAAAVSGAVADVRALLEPTP

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=441, Percent_Identity=26.7573696145125, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI8659555, Length=454, Percent_Identity=24.6696035242291, Blast_Score=97, Evalue=5e-20,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=53.1317494600432, Blast_Score=480, Evalue=1e-137,
Organism=Escherichia coli, GI1787531, Length=359, Percent_Identity=26.4623955431755, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081781, Length=356, Percent_Identity=24.1573033707865, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI2367097, Length=342, Percent_Identity=23.3918128654971, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI32564738, Length=410, Percent_Identity=29.0243902439024, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI25149337, Length=410, Percent_Identity=29.0243902439024, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=318, Percent_Identity=29.2452830188679, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17568399, Length=409, Percent_Identity=25.6723716381418, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=51.063829787234, Blast_Score=470, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6322261, Length=409, Percent_Identity=28.361858190709, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320440, Length=429, Percent_Identity=26.3403263403263, Blast_Score=126, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6323335, Length=414, Percent_Identity=27.0531400966184, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI161076999, Length=437, Percent_Identity=26.7734553775744, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI281365315, Length=437, Percent_Identity=26.7734553775744, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17864292, Length=437, Percent_Identity=26.7734553775744, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28571643, Length=438, Percent_Identity=25.7990867579909, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17137564, Length=380, Percent_Identity=25.5263157894737, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24645686, Length=384, Percent_Identity=25.5208333333333, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_GLOVI (Q7NFV7)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926363.1
- ProteinModelPortal:   Q7NFV7
- SMR:   Q7NFV7
- GeneID:   2601821
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.3417
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   GVIO251221:GLR3417-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51117; Mature: 50985

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSRATLFDKVWDLHTVGTLASGQTQLFIGLHLIHEVTSPQAFAMLRERGLGVLYAERT
CCCCHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCHHHHHHHHHCCCEEEEECHH
VATVDHIVPTQSQQRPFADTLAEQMIRALEDNCAEFGIRFYNIGSGSQGIVHVIAPEQGF
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCEEEEEECCCCC
TQPGMTIACGDSHTATHGAFGAIAFGIGTSQVRDVLATQTLALDKLKVRRIEMNGSLHPG
CCCCCEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCC
VFAKDVILHIIRKLGVKAGVGYAYEFAGTTFEAMNMEERMTVCNMAIEGGARCGYINPDA
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCC
VTYAYLKGRDFAPQGADWERAVAWWENLRSDPDAQYDDTIHFDAAEIAPTVTWGITPGQG
EEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECHHHHCCEEEECCCCCCC
IAVDEIVPRPEELPGEDRPLAEEAYRYMDLAPGVPIVGTKVDVCFIGSCTNGRLSDLREA
CCHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEECCEEEEEEEECCCCCHHHHHHHH
ANLARGRHVAPGVKAFVVPGSERVKRQAEAEGLHEVFLEAGFEWREPGCSMCLAMNPDRL
HHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCC
EGRQLSASSSNRNFKGRQGSASGRTLLMSPAMVVAAAVSGAVADVRALLEPTP
CCCEECCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AVSRATLFDKVWDLHTVGTLASGQTQLFIGLHLIHEVTSPQAFAMLRERGLGVLYAERT
CCCHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCHHHHHHHHHCCCEEEEECHH
VATVDHIVPTQSQQRPFADTLAEQMIRALEDNCAEFGIRFYNIGSGSQGIVHVIAPEQGF
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCEEEEEECCCCC
TQPGMTIACGDSHTATHGAFGAIAFGIGTSQVRDVLATQTLALDKLKVRRIEMNGSLHPG
CCCCCEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCC
VFAKDVILHIIRKLGVKAGVGYAYEFAGTTFEAMNMEERMTVCNMAIEGGARCGYINPDA
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCC
VTYAYLKGRDFAPQGADWERAVAWWENLRSDPDAQYDDTIHFDAAEIAPTVTWGITPGQG
EEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECHHHHCCEEEECCCCCCC
IAVDEIVPRPEELPGEDRPLAEEAYRYMDLAPGVPIVGTKVDVCFIGSCTNGRLSDLREA
CCHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEECCEEEEEEEECCCCCHHHHHHHH
ANLARGRHVAPGVKAFVVPGSERVKRQAEAEGLHEVFLEAGFEWREPGCSMCLAMNPDRL
HHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCC
EGRQLSASSSNRNFKGRQGSASGRTLLMSPAMVVAAAVSGAVADVRALLEPTP
CCCEECCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292