Click here to switch to the map view.

The map label for this gene is pyk1 [H]

Identifier: 37522892

GI number: 37522892

Start: 3525214

End: 3526635

Strand: Reverse

Name: pyk1 [H]

Synonym: gll3323

Alternate gene names: 37522892

Gene position: 3526635-3525214 (Counterclockwise)

Preceding gene: 37522893

Following gene: 37522889

Centisome position: 75.69

GC content: 62.1

Gene sequence:

>1422_bases
ATGCAGCCTTTAGACCGTCGAACGAAAGTCGTCGCGACGATCGGTCCTGCGAGCAGTTCGCCGGAAGTGCTTCGGCAGCT
GGTGAAGGCAGGAATCAACGTCGCGCGCTTGAATTTTTCCCACGGCAGCTACGAAGACCACGCGCGCATGGTCAAGCTGC
TGCGGGAGGTCTCCGAGGAACTCGACACCCCGATTACGCTATTGCAGGATTTGCAGGGGCCGAAGATCCGCGTGGGCCAG
ATGCCCGAGGGCGGGCTGGCTTTAGGGGAGGGCGACGAGCTGACGCTGTTGCCGGTGGCGGAATTTGCGGGCGAACCCGG
CACGGTATCCATCGACTACCCGTATCTTGTGGAGGAGGCGACGCCGGGTACCCAGGTGCTCCTGGACGACGGCTTGCTCG
AATTTGCCGTGGTTGAGGTGAAGGGCAACGCCGTGCGCTGCCGGGTGGTGCGCGGGGGGGTGCTCAAAAGCCGCAAGGGG
GTCAACCTGCCCAGTCTGGATTTGCGCCTGCCCTCGATGACCGAAAAGGACAAGCAAGATCTCGAATTTGGACTGGAGCA
GGGGGTCGATTGGGTGTCTTTGAGCTTCGTGCGCCGGGGCGAGGACATTCGCGTGCTCAAAGGACTGCTCGCTGAACACG
GGGCCGCCGATGTGCCGGTGATCGCCAAGATTGAGAAACCCCAGGCGATCGCCAATCTGGAGGAGATTCTCGATGAAGTG
CAGGGAATCATGGTGGCCAGGGGCGATCTGGGCGTTGAGATGAGCCCGGAGAAGGTGCCGCTGCTGCAAAAGCGGATCAT
TCGGCTTTGTAACCGGCGGGGCATTCCGGTGATCACCGCCACCCAGATGCTCGACAGCATGATCCGCAATCCCCGGCCGA
CCCGCGCTGAGGCGAGTGATGTCGCCAACGCCATCCTGGACGGCAGCGACGCGGTGATGCTCTCGGGCGAATCGGCGGTG
GGCGATTTTCCGGTGCAGTCGGTGGAGATGCTCGTGCGCATCGCCAAAGAAGTCGAGCGGGAGTTTACCTTCGACAACAA
TCCGCCCGCCAAATCCGATGAAATCCACGCCCTGTGCGAATCGCTGGGCACCATCGACAAGATCTTGGCCCTGCACTGTA
TTGCCACTTTTACTACCACCGGCTTCAGCGCTTCGATCGCGGCCGCGGAGCGGCCGAAGGCGCCGGTGGTCGCCTTCACC
CCCGACTTGAAGGTGTATCACCGGTTGAATCTGGTCTGGGGCGTGAAGCCCTTGCTGTTGGAGCACCCGGCGGCGGAACT
CGAAAAACTGATCGAGCAGATGGAAAGCTGCCTTGTCGAACGCAACCTCGCCGCCCCCGGCGACCGGATGCTGGTGCTGG
GAGGTAGCCCCGTCCAGAGGGCGGGCGGCACGAATTTTCTGAAGATCCATACGCTGCACTAA

Upstream 100 bases:

>100_bases
CTCGCCGGGTGAGGAAGAGCAATTGCAGCGCTCGGCCCGGGTGATGCGCCAGACTCTCGACGGGATCCAGTTTTAACCCT
AATTTTCGAGGTAATGCGTT

Downstream 100 bases:

>100_bases
AGCGGTTTTCAATGGCCTAGATAACGGCAACCTTGACGCGCAATGGTTTCAATCCAAACACTGACCTGGCAGTTGGTTTG
GCACGACAATCTGTGGGTGC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 1 [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MQPLDRRTKVVATIGPASSSPEVLRQLVKAGINVARLNFSHGSYEDHARMVKLLREVSEELDTPITLLQDLQGPKIRVGQ
MPEGGLALGEGDELTLLPVAEFAGEPGTVSIDYPYLVEEATPGTQVLLDDGLLEFAVVEVKGNAVRCRVVRGGVLKSRKG
VNLPSLDLRLPSMTEKDKQDLEFGLEQGVDWVSLSFVRRGEDIRVLKGLLAEHGAADVPVIAKIEKPQAIANLEEILDEV
QGIMVARGDLGVEMSPEKVPLLQKRIIRLCNRRGIPVITATQMLDSMIRNPRPTRAEASDVANAILDGSDAVMLSGESAV
GDFPVQSVEMLVRIAKEVEREFTFDNNPPAKSDEIHALCESLGTIDKILALHCIATFTTTGFSASIAAAERPKAPVVAFT
PDLKVYHRLNLVWGVKPLLLEHPAAELEKLIEQMESCLVERNLAAPGDRMLVLGGSPVQRAGGTNFLKIHTLH

Sequences:

>Translated_473_residues
MQPLDRRTKVVATIGPASSSPEVLRQLVKAGINVARLNFSHGSYEDHARMVKLLREVSEELDTPITLLQDLQGPKIRVGQ
MPEGGLALGEGDELTLLPVAEFAGEPGTVSIDYPYLVEEATPGTQVLLDDGLLEFAVVEVKGNAVRCRVVRGGVLKSRKG
VNLPSLDLRLPSMTEKDKQDLEFGLEQGVDWVSLSFVRRGEDIRVLKGLLAEHGAADVPVIAKIEKPQAIANLEEILDEV
QGIMVARGDLGVEMSPEKVPLLQKRIIRLCNRRGIPVITATQMLDSMIRNPRPTRAEASDVANAILDGSDAVMLSGESAV
GDFPVQSVEMLVRIAKEVEREFTFDNNPPAKSDEIHALCESLGTIDKILALHCIATFTTTGFSASIAAAERPKAPVVAFT
PDLKVYHRLNLVWGVKPLLLEHPAAELEKLIEQMESCLVERNLAAPGDRMLVLGGSPVQRAGGTNFLKIHTLH
>Mature_473_residues
MQPLDRRTKVVATIGPASSSPEVLRQLVKAGINVARLNFSHGSYEDHARMVKLLREVSEELDTPITLLQDLQGPKIRVGQ
MPEGGLALGEGDELTLLPVAEFAGEPGTVSIDYPYLVEEATPGTQVLLDDGLLEFAVVEVKGNAVRCRVVRGGVLKSRKG
VNLPSLDLRLPSMTEKDKQDLEFGLEQGVDWVSLSFVRRGEDIRVLKGLLAEHGAADVPVIAKIEKPQAIANLEEILDEV
QGIMVARGDLGVEMSPEKVPLLQKRIIRLCNRRGIPVITATQMLDSMIRNPRPTRAEASDVANAILDGSDAVMLSGESAV
GDFPVQSVEMLVRIAKEVEREFTFDNNPPAKSDEIHALCESLGTIDKILALHCIATFTTTGFSASIAAAERPKAPVVAFT
PDLKVYHRLNLVWGVKPLLLEHPAAELEKLIEQMESCLVERNLAAPGDRMLVLGGSPVQRAGGTNFLKIHTLH

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=438, Percent_Identity=42.4657534246575, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI32967597, Length=438, Percent_Identity=42.4657534246575, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI33286422, Length=443, Percent_Identity=40.8577878103837, Blast_Score=311, Evalue=8e-85,
Organism=Homo sapiens, GI33286420, Length=443, Percent_Identity=40.8577878103837, Blast_Score=311, Evalue=8e-85,
Organism=Homo sapiens, GI33286418, Length=443, Percent_Identity=40.1805869074492, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=40.9722222222222, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=40.9722222222222, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=37.2881355932203, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=37.2881355932203, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=35.8139534883721, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1787965, Length=475, Percent_Identity=40.8421052631579, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1788160, Length=482, Percent_Identity=35.2697095435685, Blast_Score=268, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI17544584, Length=483, Percent_Identity=39.5445134575569, Blast_Score=331, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI17506829, Length=489, Percent_Identity=40.4907975460123, Blast_Score=317, Evalue=9e-87,
Organism=Caenorhabditis elegans, GI71984406, Length=489, Percent_Identity=40.4907975460123, Blast_Score=317, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI71984413, Length=489, Percent_Identity=40.4907975460123, Blast_Score=317, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17506831, Length=489, Percent_Identity=40.4907975460123, Blast_Score=316, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6324923, Length=430, Percent_Identity=38.1395348837209, Blast_Score=281, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=35.523613963039, Blast_Score=281, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24648964, Length=499, Percent_Identity=38.6773547094188, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI28571814, Length=499, Percent_Identity=38.6773547094188, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI24648966, Length=411, Percent_Identity=40.3892944038929, Blast_Score=288, Evalue=6e-78,
Organism=Drosophila melanogaster, GI24581235, Length=429, Percent_Identity=32.6340326340326, Blast_Score=241, Evalue=7e-64,
Organism=Drosophila melanogaster, GI24646914, Length=264, Percent_Identity=40.530303030303, Blast_Score=179, Evalue=3e-45,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51409; Mature: 51409

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPLDRRTKVVATIGPASSSPEVLRQLVKAGINVARLNFSHGSYEDHARMVKLLREVSEE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LDTPITLLQDLQGPKIRVGQMPEGGLALGEGDELTLLPVAEFAGEPGTVSIDYPYLVEEA
HCCHHHHHHHCCCCCEEECCCCCCCEEECCCCCEEEEEEHHHCCCCCEEEECCCEEEECC
TPGTQVLLDDGLLEFAVVEVKGNAVRCRVVRGGVLKSRKGVNLPSLDLRLPSMTEKDKQD
CCCCEEEEECCCEEEEEEEEECCEEEEEEEECCCHHCCCCCCCCCCEEECCCCCCCHHHH
LEFGLEQGVDWVSLSFVRRGEDIRVLKGLLAEHGAADVPVIAKIEKPQAIANLEEILDEV
HHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHH
QGIMVARGDLGVEMSPEKVPLLQKRIIRLCNRRGIPVITATQMLDSMIRNPRPTRAEASD
HCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCCCCCHHHH
VANAILDGSDAVMLSGESAVGDFPVQSVEMLVRIAKEVEREFTFDNNPPAKSDEIHALCE
HHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
SLGTIDKILALHCIATFTTTGFSASIAAAERPKAPVVAFTPDLKVYHRLNLVWGVKPLLL
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCCCEEHHHHHHHHCCCHHHH
EHPAAELEKLIEQMESCLVERNLAAPGDRMLVLGGSPVQRAGGTNFLKIHTLH
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHCCCCCEEEEEECC
>Mature Secondary Structure
MQPLDRRTKVVATIGPASSSPEVLRQLVKAGINVARLNFSHGSYEDHARMVKLLREVSEE
CCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LDTPITLLQDLQGPKIRVGQMPEGGLALGEGDELTLLPVAEFAGEPGTVSIDYPYLVEEA
HCCHHHHHHHCCCCCEEECCCCCCCEEECCCCCEEEEEEHHHCCCCCEEEECCCEEEECC
TPGTQVLLDDGLLEFAVVEVKGNAVRCRVVRGGVLKSRKGVNLPSLDLRLPSMTEKDKQD
CCCCEEEEECCCEEEEEEEEECCEEEEEEEECCCHHCCCCCCCCCCEEECCCCCCCHHHH
LEFGLEQGVDWVSLSFVRRGEDIRVLKGLLAEHGAADVPVIAKIEKPQAIANLEEILDEV
HHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHH
QGIMVARGDLGVEMSPEKVPLLQKRIIRLCNRRGIPVITATQMLDSMIRNPRPTRAEASD
HCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCCCCCHHHH
VANAILDGSDAVMLSGESAVGDFPVQSVEMLVRIAKEVEREFTFDNNPPAKSDEIHALCE
HHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
SLGTIDKILALHCIATFTTTGFSASIAAAERPKAPVVAFTPDLKVYHRLNLVWGVKPLLL
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCCCEEHHHHHHHHCCCHHHH
EHPAAELEKLIEQMESCLVERNLAAPGDRMLVLGGSPVQRAGGTNFLKIHTLH
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]