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The map label for this gene is ispH

Identifier: 37522868

GI number: 37522868

Start: 3499663

End: 3500916

Strand: Direct

Name: ispH

Synonym: glr3299

Alternate gene names: 37522868

Gene position: 3499663-3500916 (Clockwise)

Preceding gene: 37522867

Following gene: 37522870

Centisome position: 75.12

GC content: 62.68

Gene sequence:

>1254_bases
ATGCAGTCCAAAAATTACTTCCGCAAGGGTTTCGGTCTTAAAGCCGAGGTACAGGACGATCTCAAGGCGGAGTACGAATC
TTCGCTCATCCACGAAATTCGCGCCAACGGCTACACACTGCGGCGCGGCGGGGTGACCGTCCGCCTGGCGGAGGCGTTCG
GCTTTTGCTGGGGGGTCGATAAGGCGGTGTCGATGGCCTACGAGACACACCGCATGTTTGCTGGCCGCCAGCTGTGGATC
ACCAACGAGATCATCCACAACCCGCTGGTCAATCAGCACCTGCGCGCGATGGGCATCCGCTTTCTCGACGAAGACGAAAG
CGGCCGGCGCGTGCCCAAGGATTTTGAGCGCGTGAGCGAGAAGGACGTGGTGATCTTGCCCGCCTTCGGCGCCTCTACCC
AGGAGATGCAGATCCTTGACGAGAAAAACTGCGTCATCGTCGATACCACCTGCCCCTGGGTCTCGGCGGTCTGGAACCGG
GTGGGCAAGTACGATCGGGCCGAATTCACCTCGATCATCCACGGCAAGTACCAGCACGAAGAGACGGTGGCGACCGCTTC
GCGGGCCCGCCGCTATCTGGTGGTGCTCAACCTGGAGGAGGCCCAGCAGGTGTGCGACTATATCCTCCATGGCGGCGATC
GCCGGGCGTTTTTGGCCCACTTCGGCATGGCGGCTTGCGAGGGCTTCGACCCCGACCGCGACCTGGTGCGCGTCGGCATC
GCCAATCAGACCACGATGCTCAAGGGCGAGACCGAGCGCATCGGCAAGTTGTTCGAGCGCACGATGATGCGCCGCTACGG
CCCCGCAAACCTGGCCGACCACTTCATGAGCCACGACACGATCTGCGACGCCACCCAGGAGCGCCAGGACGCCATGTTCA
AGCTCGTCGAGGAGCCGCTCGATCTGCTGGTCGTCATCGGCGGCTACAACTCCTCCAACACCACCCACCTGCAGGAGATT
GCCGTCGAGCGGGGTTTGCCATCTTTTCACATCGACGGGGCTGACCGTCTGGTCTCGGCGCAATTTATCGAGCACCGCGA
CTTGCACAGCAAATCGCTGGTGCGCACCAAAAACTGGCTTCCGGCCGGAGAAGTGACCGTCGGGGTAACCGCCGGGGCTT
CCACACCCGACCGGGTGGTAGCCGAGGTGATCGCCCGGATTTTTGAACTGAAGGGCGTAGGCGAGTCGGGCGCCACCGGG
GCAATATCCTCCCCGGTGCTCTCAAGCGCCGAGGGTGGCCCTTCGCTTTCGTAG

Upstream 100 bases:

>100_bases
GGAGACCGGGTTGTGGACACCGAAGACCGGCCGCTCGCCGCTCCCGGCTTCGAGCACTTCAATCGACCGTAAAATGGCGG
TGTAGGCCCTGGAGTCGCCG

Downstream 100 bases:

>100_bases
CGCGGCTCTCTCAACCGACGCTGTAGGCGGGAACGGCTTCGCGGGCGGCGGGGGGCAGGTGGTGGAACCAGCCGGTCAGG
GCGATGGCCAGGGCATCGGC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MQSKNYFRKGFGLKAEVQDDLKAEYESSLIHEIRANGYTLRRGGVTVRLAEAFGFCWGVDKAVSMAYETHRMFAGRQLWI
TNEIIHNPLVNQHLRAMGIRFLDEDESGRRVPKDFERVSEKDVVILPAFGASTQEMQILDEKNCVIVDTTCPWVSAVWNR
VGKYDRAEFTSIIHGKYQHEETVATASRARRYLVVLNLEEAQQVCDYILHGGDRRAFLAHFGMAACEGFDPDRDLVRVGI
ANQTTMLKGETERIGKLFERTMMRRYGPANLADHFMSHDTICDATQERQDAMFKLVEEPLDLLVVIGGYNSSNTTHLQEI
AVERGLPSFHIDGADRLVSAQFIEHRDLHSKSLVRTKNWLPAGEVTVGVTAGASTPDRVVAEVIARIFELKGVGESGATG
AISSPVLSSAEGGPSLS

Sequences:

>Translated_417_residues
MQSKNYFRKGFGLKAEVQDDLKAEYESSLIHEIRANGYTLRRGGVTVRLAEAFGFCWGVDKAVSMAYETHRMFAGRQLWI
TNEIIHNPLVNQHLRAMGIRFLDEDESGRRVPKDFERVSEKDVVILPAFGASTQEMQILDEKNCVIVDTTCPWVSAVWNR
VGKYDRAEFTSIIHGKYQHEETVATASRARRYLVVLNLEEAQQVCDYILHGGDRRAFLAHFGMAACEGFDPDRDLVRVGI
ANQTTMLKGETERIGKLFERTMMRRYGPANLADHFMSHDTICDATQERQDAMFKLVEEPLDLLVVIGGYNSSNTTHLQEI
AVERGLPSFHIDGADRLVSAQFIEHRDLHSKSLVRTKNWLPAGEVTVGVTAGASTPDRVVAEVIARIFELKGVGESGATG
AISSPVLSSAEGGPSLS
>Mature_417_residues
MQSKNYFRKGFGLKAEVQDDLKAEYESSLIHEIRANGYTLRRGGVTVRLAEAFGFCWGVDKAVSMAYETHRMFAGRQLWI
TNEIIHNPLVNQHLRAMGIRFLDEDESGRRVPKDFERVSEKDVVILPAFGASTQEMQILDEKNCVIVDTTCPWVSAVWNR
VGKYDRAEFTSIIHGKYQHEETVATASRARRYLVVLNLEEAQQVCDYILHGGDRRAFLAHFGMAACEGFDPDRDLVRVGI
ANQTTMLKGETERIGKLFERTMMRRYGPANLADHFMSHDTICDATQERQDAMFKLVEEPLDLLVVIGGYNSSNTTHLQEI
AVERGLPSFHIDGADRLVSAQFIEHRDLHSKSLVRTKNWLPAGEVTVGVTAGASTPDRVVAEVIARIFELKGVGESGATG
AISSPVLSSAEGGPSLS

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=351, Percent_Identity=26.2108262108262, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_GLOVI (Q7NG74)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926245.1
- GeneID:   2601701
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:glr3299
- NMPDR:   fig|251221.1.peg.3299
- HOGENOM:   HBG286221
- OMA:   FLLSEMI
- ProtClustDB:   PRK13371
- BioCyc:   GVIO251221:GLR3299-MONOMER
- BRENDA:   1.17.1.2
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 46492; Mature: 46492

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSKNYFRKGFGLKAEVQDDLKAEYESSLIHEIRANGYTLRRGGVTVRLAEAFGFCWGVD
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEHHHHHHHHHHH
KAVSMAYETHRMFAGRQLWITNEIIHNPLVNQHLRAMGIRFLDEDESGRRVPKDFERVSE
HHHHHHHHHHHHHCCCEEEEEHHHHHCHHHHHHHHHHCCEEECCCCCCCCCCHHHHHCCC
KDVVILPAFGASTQEMQILDEKNCVIVDTTCPWVSAVWNRVGKYDRAEFTSIIHGKYQHE
CCEEEEECCCCCCHHHHEECCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHH
ETVATASRARRYLVVLNLEEAQQVCDYILHGGDRRAFLAHFGMAACEGFDPDRDLVRVGI
HHHHHHHHCCEEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHEEEEC
ANQTTMLKGETERIGKLFERTMMRRYGPANLADHFMSHDTICDATQERQDAMFKLVEEPL
CCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
DLLVVIGGYNSSNTTHLQEIAVERGLPSFHIDGADRLVSAQFIEHRDLHSKSLVRTKNWL
EEEEEEECCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHCCCC
PAGEVTVGVTAGASTPDRVVAEVIARIFELKGVGESGATGAISSPVLSSAEGGPSLS
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCC
>Mature Secondary Structure
MQSKNYFRKGFGLKAEVQDDLKAEYESSLIHEIRANGYTLRRGGVTVRLAEAFGFCWGVD
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEHHHHHHHHHHH
KAVSMAYETHRMFAGRQLWITNEIIHNPLVNQHLRAMGIRFLDEDESGRRVPKDFERVSE
HHHHHHHHHHHHHCCCEEEEEHHHHHCHHHHHHHHHHCCEEECCCCCCCCCCHHHHHCCC
KDVVILPAFGASTQEMQILDEKNCVIVDTTCPWVSAVWNRVGKYDRAEFTSIIHGKYQHE
CCEEEEECCCCCCHHHHEECCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHH
ETVATASRARRYLVVLNLEEAQQVCDYILHGGDRRAFLAHFGMAACEGFDPDRDLVRVGI
HHHHHHHHCCEEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHEEEEC
ANQTTMLKGETERIGKLFERTMMRRYGPANLADHFMSHDTICDATQERQDAMFKLVEEPL
CCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
DLLVVIGGYNSSNTTHLQEIAVERGLPSFHIDGADRLVSAQFIEHRDLHSKSLVRTKNWL
EEEEEEECCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHCCCC
PAGEVTVGVTAGASTPDRVVAEVIARIFELKGVGESGATGAISSPVLSSAEGGPSLS
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292