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The map label for this gene is rfbA [H]

Identifier: 37522806

GI number: 37522806

Start: 3444065

End: 3445132

Strand: Direct

Name: rfbA [H]

Synonym: gvip449

Alternate gene names: 37522806

Gene position: 3444065-3445132 (Clockwise)

Preceding gene: 37522805

Following gene: 37522807

Centisome position: 73.92

GC content: 63.67

Gene sequence:

>1068_bases
ATGCGCGGACTGATCCTCTCGGGGGGCAAGGGCACCCGCCTGCGGCCCCTCACCTACACCGGCGCCAAGCAACTGGTGCC
CGTCGCCAACAAACCGATCCTCTGGTACGGCATCGAGGCGATGGTCGCCTCGGGCATCCGCGAAATCGGCATCGTCATCT
CGCCGGAGACGGGCGAACAGGTGCGCGCCCTCACCGGGGACGGCAGCCGGTTCGCAGCCCAAATCGAGTACATCCTGCAG
TCGGAGCCGGGGGGGCTCGCCCATGCCGTGCGCACCGCCCGGCCGTTTCTGGGCGAGAGTTCCTTTGCGATGTATCTGGG
GGACAACTTGATCCAGTCGGGGCTTGCGGCCCCCATCGAACGCTTCGAGGCCAGCCGCCCCGAGGCGCTGATTTTGCTCA
AGCGTGTCAGCAACCCGACTGCCTTCGGGGTGGCCGAACTCGACGGGCAAGGGCGGGTGATGCGGCTGGTCGAAAAGCCC
AAAATACCGCCTTCGGATCTGGCGTTGGTGGGGGTCTACTTGTTTTCGTCCCAGATCCACGCGGCCATCGATGCGATCCG
CCCTTCGGCGCGCGGCGAGCTGGAAATTACCGATGCCATCCAGCAGCTGATCGAAGTGGGCCAGCGTGTGGAGTCGGTGG
AATTGACCGGCTGGTGGCTCGATACCGGCAAAAAAGACGATCTTTTAGAAGCCAACCGCGTCATCCTCGACGAACAGATG
CACTGCCCGATGGAAGGGTCGCTCGATGAGGCGAGCAAAGTGAGCGGGCGCGTCTGCATCGGCAAGGGAGCACGGCTGGT
GCGCTCGATGGTGCGCGGTCCGGTCACCATCGGTCCTGAGTGCCTGATCGAAGACAGTTACATCGGCCCTTACACGAGCA
TCGCAGGCGGTGTCCAGCTCAAGAATGTCGACATCGAGCACAGCGTCGTGATGGAAAAAACCTGCATCAGCGGCGCCGGC
CGCATCGTGGACAGCCTCATCGGCCAGCGCACGGTGATCGCCCGCCACAACGGCAAACCGGAGGCCCATCGCTTCTTGCT
CGGGGACGATTCGCTCATTGAGCTATAA

Upstream 100 bases:

>100_bases
GCATGGACATCCGGTACATTCGCACCTGGTCGCTGGCGCTCGATTTCGCCATATTGATCAAGACCGTAGGCGCGGTGGTG
AGAGCCCGGGGAGCCTACTG

Downstream 100 bases:

>100_bases
CTATACGTAATGAAAGCGGTTGTTTCTATTTCGAGTCCAGTTTTTCGCGAAGCTGAGCTCCTGGCAAAGCGCCTGGGCAT
TTCGCGCAGCGAGTTGTATT

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: Sugar-nucleotidylation enzyme; dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MRGLILSGGKGTRLRPLTYTGAKQLVPVANKPILWYGIEAMVASGIREIGIVISPETGEQVRALTGDGSRFAAQIEYILQ
SEPGGLAHAVRTARPFLGESSFAMYLGDNLIQSGLAAPIERFEASRPEALILLKRVSNPTAFGVAELDGQGRVMRLVEKP
KIPPSDLALVGVYLFSSQIHAAIDAIRPSARGELEITDAIQQLIEVGQRVESVELTGWWLDTGKKDDLLEANRVILDEQM
HCPMEGSLDEASKVSGRVCIGKGARLVRSMVRGPVTIGPECLIEDSYIGPYTSIAGGVQLKNVDIEHSVVMEKTCISGAG
RIVDSLIGQRTVIARHNGKPEAHRFLLGDDSLIEL

Sequences:

>Translated_355_residues
MRGLILSGGKGTRLRPLTYTGAKQLVPVANKPILWYGIEAMVASGIREIGIVISPETGEQVRALTGDGSRFAAQIEYILQ
SEPGGLAHAVRTARPFLGESSFAMYLGDNLIQSGLAAPIERFEASRPEALILLKRVSNPTAFGVAELDGQGRVMRLVEKP
KIPPSDLALVGVYLFSSQIHAAIDAIRPSARGELEITDAIQQLIEVGQRVESVELTGWWLDTGKKDDLLEANRVILDEQM
HCPMEGSLDEASKVSGRVCIGKGARLVRSMVRGPVTIGPECLIEDSYIGPYTSIAGGVQLKNVDIEHSVVMEKTCISGAG
RIVDSLIGQRTVIARHNGKPEAHRFLLGDDSLIEL
>Mature_355_residues
MRGLILSGGKGTRLRPLTYTGAKQLVPVANKPILWYGIEAMVASGIREIGIVISPETGEQVRALTGDGSRFAAQIEYILQ
SEPGGLAHAVRTARPFLGESSFAMYLGDNLIQSGLAAPIERFEASRPEALILLKRVSNPTAFGVAELDGQGRVMRLVEKP
KIPPSDLALVGVYLFSSQIHAAIDAIRPSARGELEITDAIQQLIEVGQRVESVELTGWWLDTGKKDDLLEANRVILDEQM
HCPMEGSLDEASKVSGRVCIGKGARLVRSMVRGPVTIGPECLIEDSYIGPYTSIAGGVQLKNVDIEHSVVMEKTCISGAG
RIVDSLIGQRTVIARHNGKPEAHRFLLGDDSLIEL

Specific function: Involved in the biosynthesis of the streptose moiety of streptomycin. Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=289, Percent_Identity=28.3737024221453, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI11761619, Length=289, Percent_Identity=28.3737024221453, Blast_Score=111, Evalue=9e-25,
Organism=Escherichia coli, GI1790224, Length=239, Percent_Identity=41.0041841004184, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1788351, Length=252, Percent_Identity=35.3174603174603, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1787488, Length=269, Percent_Identity=28.996282527881, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788355, Length=272, Percent_Identity=29.0441176470588, Blast_Score=94, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI133931050, Length=336, Percent_Identity=28.2738095238095, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17509979, Length=354, Percent_Identity=25.7062146892655, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17509981, Length=343, Percent_Identity=25.3644314868805, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320148, Length=339, Percent_Identity=25.0737463126844, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21355443, Length=339, Percent_Identity=27.4336283185841, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24644084, Length=339, Percent_Identity=27.4336283185841, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005908
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 38296; Mature: 38296

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLILSGGKGTRLRPLTYTGAKQLVPVANKPILWYGIEAMVASGIREIGIVISPETGEQ
CCCEEEECCCCCEEEEEEECCCHHCCCCCCCCEEEEEHHHHHHCCHHHCCEEECCCCCCC
VRALTGDGSRFAAQIEYILQSEPGGLAHAVRTARPFLGESSFAMYLGDNLIQSGLAAPIE
EEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHH
RFEASRPEALILLKRVSNPTAFGVAELDGQGRVMRLVEKPKIPPSDLALVGVYLFSSQIH
HHCCCCCCEEEEEEECCCCCEEEEEEECCCCCEEHHHHCCCCCCCHHHHHHHHHHHHHHH
AAIDAIRPSARGELEITDAIQQLIEVGQRVESVELTGWWLDTGKKDDLLEANRVILDEQM
HHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCHHHHCCEEECCCC
HCPMEGSLDEASKVSGRVCIGKGARLVRSMVRGPVTIGPECLIEDSYIGPYTSIAGGVQL
CCCCCCCCCHHHHCCCEEEECCCHHHHHHHHCCCCEECCHHEEECCCCCCHHHHCCCEEE
KNVDIEHSVVMEKTCISGAGRIVDSLIGQRTVIARHNGKPEAHRFLLGDDSLIEL
EECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHEEEECCCHHCCC
>Mature Secondary Structure
MRGLILSGGKGTRLRPLTYTGAKQLVPVANKPILWYGIEAMVASGIREIGIVISPETGEQ
CCCEEEECCCCCEEEEEEECCCHHCCCCCCCCEEEEEHHHHHHCCHHHCCEEECCCCCCC
VRALTGDGSRFAAQIEYILQSEPGGLAHAVRTARPFLGESSFAMYLGDNLIQSGLAAPIE
EEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHH
RFEASRPEALILLKRVSNPTAFGVAELDGQGRVMRLVEKPKIPPSDLALVGVYLFSSQIH
HHCCCCCCEEEEEEECCCCCEEEEEEECCCCCEEHHHHCCCCCCCHHHHHHHHHHHHHHH
AAIDAIRPSARGELEITDAIQQLIEVGQRVESVELTGWWLDTGKKDDLLEANRVILDEQM
HHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCHHHHCCEEECCCC
HCPMEGSLDEASKVSGRVCIGKGARLVRSMVRGPVTIGPECLIEDSYIGPYTSIAGGVQL
CCCCCCCCCHHHHCCCEEEECCCHHHHHHHHCCCCEECCHHEEECCCCCCHHHHCCCEEE
KNVDIEHSVVMEKTCISGAGRIVDSLIGQRTVIARHNGKPEAHRFLLGDDSLIEL
EECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3118332 [H]