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The map label for this gene is appA [H]

Identifier: 37522773

GI number: 37522773

Start: 3411095

End: 3413092

Strand: Direct

Name: appA [H]

Synonym: glr3204

Alternate gene names: 37522773

Gene position: 3411095-3413092 (Clockwise)

Preceding gene: 37522770

Following gene: 37522774

Centisome position: 73.21

GC content: 65.87

Gene sequence:

>1998_bases
GTGGCGCAGCGGCAGGGAACGGGCAGTGCGAGAAGCCATATAGCCCCCGAGGCCAGTAAAAAACAGTCCGCGCAAAAAAT
TGAAGACAAAAAAGGCGGCCGTGCCCTCGACGGGATACCCGCTCAGCATCACCACCAGACCCGCCGCCATGTACGTAACC
AGTTCGGAGTGCAGCGTACCGGCGACATCGACCAGCGAACCAGTAAATACGGCTCGCGACTCGAGGGGGCGGGGGGCCAG
ATCGGCGCTGTTGCGGGGTTCGAGCATGGTGATCGCCGGGAAGAGGGAAGATACGAAAATTATAGGCCGCCCGAAGCTTT
CGGGTGGGTGGGCGCTTCCATGGCTAGGACGGCGGCGAGTGTGATAAGTTTCCTGCTGATGGCTAGGATTTTTGCGTTTG
CCGCCGCCTTGCTGCTCGCAGGCTGCACCTCCACCTCCCTGCCGCTTACACCCAAACCGGTGACGGGCGGCGTCCTCTCC
GGGACCCTCATCGGCGATCCAGGCACCTTCAACCCGGCCCTCGCCCGGCGCGGCAGCGCCCTGGAGGTTAGTCGGCTGAT
GTTTGCCGGGTTGCTGATGCCCGATAAAGCCGGTGGTTTCTCCCCCGACCTGGCTGCCGACTTTCAGCAGGCCGAGGACG
GCCGCCGCTACCGCGTCCGACTCAAACCCGCACTGGTCTGGTCCAATGGCCAACCGCTCGGCGCGAACGATGTCGTGTTC
TCCTTCGAGCGGGTCTACGGCGACGGGCGATCTCCAGGCAGTCTGCCCCGGGGACTGAAGGTGAGCGCCCCGGACGAGCA
AACGGTCGAATTTGTCCTGCCGGCCCCCGATTTCGATTTTGCCGCCTACCTGACGCTGCCGATTCTGCCCGCCCACGCCC
TGGCCGAAGGCGATCCCCTCGCCCGGTGGGGACTCGACGCCGACCCGACCACCCTGCCGGTCAGCGGGCCGTTTCTGTGC
CGCGGCTATACGCCCAACGAGAGTCTGGAACTGGCGGCCAATCCCCGCTACTGGCGCCGGCCCGAGCAACCTTATCTGGC
GGGCATTCGCTACCGGATCGTGCCGGATCGGCCCGCGGCCCTTGCCCGCTTCCAGAGCGGCCAGAGCGACACCTACCGGC
CGAGCCCCGAAGAGTATGCTGGGCTGGCGGCCGGGCGCTCTTCGGGTAACTACGCCCTGGTGAGCGGTGGCGCCGAGCAG
CCTGTTTATCTTGCCTTCAACCTCAATCGGACCGCAGTTGCTGGCAAAGCGCCCGTCGCCTCCCCTGGGGCGAACTGGTT
CGCCAACCCCGCTTTTCGCCAGGGGGTTGCCCTCGCCATCGATCGCAGCCGGCTGGTTCAAGAAGCTTTCTTGAACACGG
CCGAGGCCGGCAAAGGCACATTCGATCAGGACGCAGCCCGCGAGCGGCTCGCCGCCGCCGGTCTCAAGGGCGGTGCAGGC
GGAGTGCTCACCGATGCGGCGGGCCATAAAGTCAGCTTCGAGGTCTTGCTCGACGGCGACGATCCGGCCCAGATGCGGCT
GGGCGAGGGCGTGCGCGCCCAACTGGCGCTTGTCGGTCTCGATGCCCAACCGCGCCCCCTGCCCCGGCTGGTGCTGCGCA
ACCGCATCTTGCGCCAAAAACGCTGGCAGGCTGCAATCACCAATCCCGAGCGACTGGGGGCGCCGCCCCCGGCGAGCCTC
TCGTTCTGGCGATCCGCGAACCCATGGCATTTTTTTGACCTGGGCGACGACCAGGGCACTCTCCCCTGGCAGGACCAATT
CGATGCCACGTATGGGAACAAAACCGGTGCGTCCGAGCGCGAACAATTGATAGCGGCGCACCAGCCGCTCGTCGAACTGG
TGCGTCCCCTGGCGCTCGCCGCCATTCGGGCCGATGTACGCGAAAGTCGCTTCAGTGCCTTCGAGAGCACCTACAACGGC
CGCATCCTCGCTAACATTTGGGAGGTGCAAAAAGCACCGCCGCCCCCGGCAGAAGTCTCCCCGGCACCGGGCAGCTGA

Upstream 100 bases:

>100_bases
CCAACAAACCTAGCACCAGCGGCCAGGAGAGCAGTCCTCCGGGAGCATCCAGATTGAAGAAGATTGAAACCGCCAGCGAA
ATGATCCCAGTCAGCAGCGC

Downstream 100 bases:

>100_bases
AGACAAACCGGACCGGATTGCGAGGATGAAATGATCTACATCGTTGCCATGGTTGCCGCCCTGGTCTGCGCCTTGAGCCT
CGTCCAGTGGCAAAGGCAAC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 665; Mature: 664

Protein sequence:

>665_residues
MAQRQGTGSARSHIAPEASKKQSAQKIEDKKGGRALDGIPAQHHHQTRRHVRNQFGVQRTGDIDQRTSKYGSRLEGAGGQ
IGAVAGFEHGDRREEGRYENYRPPEAFGWVGASMARTAASVISFLLMARIFAFAAALLLAGCTSTSLPLTPKPVTGGVLS
GTLIGDPGTFNPALARRGSALEVSRLMFAGLLMPDKAGGFSPDLAADFQQAEDGRRYRVRLKPALVWSNGQPLGANDVVF
SFERVYGDGRSPGSLPRGLKVSAPDEQTVEFVLPAPDFDFAAYLTLPILPAHALAEGDPLARWGLDADPTTLPVSGPFLC
RGYTPNESLELAANPRYWRRPEQPYLAGIRYRIVPDRPAALARFQSGQSDTYRPSPEEYAGLAAGRSSGNYALVSGGAEQ
PVYLAFNLNRTAVAGKAPVASPGANWFANPAFRQGVALAIDRSRLVQEAFLNTAEAGKGTFDQDAARERLAAAGLKGGAG
GVLTDAAGHKVSFEVLLDGDDPAQMRLGEGVRAQLALVGLDAQPRPLPRLVLRNRILRQKRWQAAITNPERLGAPPPASL
SFWRSANPWHFFDLGDDQGTLPWQDQFDATYGNKTGASEREQLIAAHQPLVELVRPLALAAIRADVRESRFSAFESTYNG
RILANIWEVQKAPPPPAEVSPAPGS

Sequences:

>Translated_665_residues
MAQRQGTGSARSHIAPEASKKQSAQKIEDKKGGRALDGIPAQHHHQTRRHVRNQFGVQRTGDIDQRTSKYGSRLEGAGGQ
IGAVAGFEHGDRREEGRYENYRPPEAFGWVGASMARTAASVISFLLMARIFAFAAALLLAGCTSTSLPLTPKPVTGGVLS
GTLIGDPGTFNPALARRGSALEVSRLMFAGLLMPDKAGGFSPDLAADFQQAEDGRRYRVRLKPALVWSNGQPLGANDVVF
SFERVYGDGRSPGSLPRGLKVSAPDEQTVEFVLPAPDFDFAAYLTLPILPAHALAEGDPLARWGLDADPTTLPVSGPFLC
RGYTPNESLELAANPRYWRRPEQPYLAGIRYRIVPDRPAALARFQSGQSDTYRPSPEEYAGLAAGRSSGNYALVSGGAEQ
PVYLAFNLNRTAVAGKAPVASPGANWFANPAFRQGVALAIDRSRLVQEAFLNTAEAGKGTFDQDAARERLAAAGLKGGAG
GVLTDAAGHKVSFEVLLDGDDPAQMRLGEGVRAQLALVGLDAQPRPLPRLVLRNRILRQKRWQAAITNPERLGAPPPASL
SFWRSANPWHFFDLGDDQGTLPWQDQFDATYGNKTGASEREQLIAAHQPLVELVRPLALAAIRADVRESRFSAFESTYNG
RILANIWEVQKAPPPPAEVSPAPGS
>Mature_664_residues
AQRQGTGSARSHIAPEASKKQSAQKIEDKKGGRALDGIPAQHHHQTRRHVRNQFGVQRTGDIDQRTSKYGSRLEGAGGQI
GAVAGFEHGDRREEGRYENYRPPEAFGWVGASMARTAASVISFLLMARIFAFAAALLLAGCTSTSLPLTPKPVTGGVLSG
TLIGDPGTFNPALARRGSALEVSRLMFAGLLMPDKAGGFSPDLAADFQQAEDGRRYRVRLKPALVWSNGQPLGANDVVFS
FERVYGDGRSPGSLPRGLKVSAPDEQTVEFVLPAPDFDFAAYLTLPILPAHALAEGDPLARWGLDADPTTLPVSGPFLCR
GYTPNESLELAANPRYWRRPEQPYLAGIRYRIVPDRPAALARFQSGQSDTYRPSPEEYAGLAAGRSSGNYALVSGGAEQP
VYLAFNLNRTAVAGKAPVASPGANWFANPAFRQGVALAIDRSRLVQEAFLNTAEAGKGTFDQDAARERLAAAGLKGGAGG
VLTDAAGHKVSFEVLLDGDDPAQMRLGEGVRAQLALVGLDAQPRPLPRLVLRNRILRQKRWQAAITNPERLGAPPPASLS
FWRSANPWHFFDLGDDQGTLPWQDQFDATYGNKTGASEREQLIAAHQPLVELVRPLALAAIRADVRESRFSAFESTYNGR
ILANIWEVQKAPPPPAEVSPAPGS

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=436, Percent_Identity=22.9357798165138, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787052, Length=407, Percent_Identity=24.5700245700246, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787762, Length=225, Percent_Identity=28.8888888888889, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 71526; Mature: 71395

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQRQGTGSARSHIAPEASKKQSAQKIEDKKGGRALDGIPAQHHHQTRRHVRNQFGVQRT
CCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
GDIDQRTSKYGSRLEGAGGQIGAVAGFEHGDRREEGRYENYRPPEAFGWVGASMARTAAS
CCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VISFLLMARIFAFAAALLLAGCTSTSLPLTPKPVTGGVLSGTLIGDPGTFNPALARRGSA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECEEECCCCCCCHHHHCCCCH
LEVSRLMFAGLLMPDKAGGFSPDLAADFQQAEDGRRYRVRLKPALVWSNGQPLGANDVVF
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCHHHE
SFERVYGDGRSPGSLPRGLKVSAPDEQTVEFVLPAPDFDFAAYLTLPILPAHALAEGDPL
EHHHHHCCCCCCCCCCCCCEECCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHCCCCCH
ARWGLDADPTTLPVSGPFLCRGYTPNESLELAANPRYWRRPEQPYLAGIRYRIVPDRPAA
HHCCCCCCCCEECCCCCEEEECCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCHHH
LARFQSGQSDTYRPSPEEYAGLAAGRSSGNYALVSGGAEQPVYLAFNLNRTAVAGKAPVA
HHHHCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCEEECCCCCCC
SPGANWFANPAFRQGVALAIDRSRLVQEAFLNTAEAGKGTFDQDAARERLAAAGLKGGAG
CCCCCCCCCHHHHCCEEEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCC
GVLTDAAGHKVSFEVLLDGDDPAQMRLGEGVRAQLALVGLDAQPRPLPRLVLRNRILRQK
CEEECCCCCEEEEEEEECCCCHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHH
RWQAAITNPERLGAPPPASLSFWRSANPWHFFDLGDDQGTLPWQDQFDATYGNKTGASER
HHHHHCCCHHHCCCCCCCCCHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHH
EQLIAAHQPLVELVRPLALAAIRADVRESRFSAFESTYNGRILANIWEVQKAPPPPAEVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCCCC
PAPGS
CCCCC
>Mature Secondary Structure 
AQRQGTGSARSHIAPEASKKQSAQKIEDKKGGRALDGIPAQHHHQTRRHVRNQFGVQRT
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
GDIDQRTSKYGSRLEGAGGQIGAVAGFEHGDRREEGRYENYRPPEAFGWVGASMARTAAS
CCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VISFLLMARIFAFAAALLLAGCTSTSLPLTPKPVTGGVLSGTLIGDPGTFNPALARRGSA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECEEECCCCCCCHHHHCCCCH
LEVSRLMFAGLLMPDKAGGFSPDLAADFQQAEDGRRYRVRLKPALVWSNGQPLGANDVVF
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCHHHE
SFERVYGDGRSPGSLPRGLKVSAPDEQTVEFVLPAPDFDFAAYLTLPILPAHALAEGDPL
EHHHHHCCCCCCCCCCCCCEECCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHCCCCCH
ARWGLDADPTTLPVSGPFLCRGYTPNESLELAANPRYWRRPEQPYLAGIRYRIVPDRPAA
HHCCCCCCCCEECCCCCEEEECCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCHHH
LARFQSGQSDTYRPSPEEYAGLAAGRSSGNYALVSGGAEQPVYLAFNLNRTAVAGKAPVA
HHHHCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCEEECCCCCCC
SPGANWFANPAFRQGVALAIDRSRLVQEAFLNTAEAGKGTFDQDAARERLAAAGLKGGAG
CCCCCCCCCHHHHCCEEEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCC
GVLTDAAGHKVSFEVLLDGDDPAQMRLGEGVRAQLALVGLDAQPRPLPRLVLRNRILRQK
CEEECCCCCEEEEEEEECCCCHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHH
RWQAAITNPERLGAPPPASLSFWRSANPWHFFDLGDDQGTLPWQDQFDATYGNKTGASER
HHHHHCCCHHHCCCCCCCCCHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHH
EQLIAAHQPLVELVRPLALAAIRADVRESRFSAFESTYNGRILANIWEVQKAPPPPAEVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCCCC
PAPGS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]