Click here to switch to the map view.

The map label for this gene is kinB [H]

Identifier: 37522691

GI number: 37522691

Start: 3324070

End: 3325146

Strand: Reverse

Name: kinB [H]

Synonym: gll3122

Alternate gene names: 37522691

Gene position: 3325146-3324070 (Counterclockwise)

Preceding gene: 37522692

Following gene: 37522688

Centisome position: 71.37

GC content: 62.86

Gene sequence:

>1077_bases
ATGATCCAGTCTGCCGAACGCATCTTGGTCGTTGACGACCTGCCTGACAACATCGCCCTGGTCCAATTTCTGCTGGCGAG
CGAGGGTTACGAAATCGAAACGGCAAGTTCCGGTGCGGAGTGCCTTGCTCGGGTTTACAAAAATTCCCCGGATCTGCTGC
TGCTTGACGTGATGATGCCCGGGATGGACGGCTACGAAGTCACCCGTCGCCTGAAGAGCGACGGGCATCTGCCCTTTTTG
CCGATTTTGCTGGTGACTGCCTCTGACCGCCCAAGTGCGGTCCTGGGTCTCGACGCTGGGGCGGACGAGTTTATCCGCAA
GCCGATCGAGCCGGACGAATTGCTCGCCCGGGTGCGTTCACTGCTGCGCCTCAAGCGGTCGATCAGCGAACGCGACAACA
TCGCCCGCCAGCGCGAAGATTTTGTGGCCCGCCTCGCCCACGACCTGCGCACCCCCCTGGTGGCCGCCGACCGCATGTTC
AACCTGCTGGGTCAGGGAGCGCTCGGACCGATGCCGCCCTCGATGGCCGAGACGGTAGACATCATGGCCCGCTCCAACAG
CAACCTGCTGGCACTGGTGCGCACGTTGCTGGATGTCTACCGCTACGAGGCCGGGGCCAAAAAGCTGCATTTTCAGCCGG
TCGATGTCATCGAATTGATGCGGCAGGTGTGCGACGAACTGGCTCCGCTCGCGGCTGAGAAGCATCTGGAGTTGTGTGCC
CAACTGGAGGCGGCCCCCAAGGTGCCGGCCGACCGTCTGGAACTGCGGCGGGTGCTGACCAATTTGCTCGGCAACGCCCT
CAAATTCACCGACCAGGGCGGCGTCACCGTGCGCAGCCGCCTAGAGGACGGCGGCTGCGTCCACCTCGATTTTTGCGATA
CCGGACCGGGGATCGCCGCGGAGGAACAGGCGTATCTGTTTCGCCGTTTTGCCCAGGGCCGCCACAACAAAGAAGGCAGC
GGCATGGGTCTGCATTTGAGCCGCCAGATTGTCGAAGCCCACGGCGGCACCCTCAGTCTCACCAGTGCTCCCGGCAAAGG
CAGTACCTTCACGGTGACGTTACCGGCCGCGAAGTGA

Upstream 100 bases:

>100_bases
TCTCTGTAATCTAATGGGGGTGCCCGCTTCTTCACGGGGGTGGCGCACGTATGTGTATTAGTGACGGTGCACCCTGTCTG
GCGCCGCCTCCTGCCCTCCT

Downstream 100 bases:

>100_bases
TCGCTTATTGGTTTCGGGGAGCAAACACCCGGCCAAGGGGCCGGGGGGGAGAAGAGAGCAGCGCGTAGGCCCGCCGCGAA
TCGAGGCTGACATCGGTCGG

Product: two-component hybrid sensor and regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MIQSAERILVVDDLPDNIALVQFLLASEGYEIETASSGAECLARVYKNSPDLLLLDVMMPGMDGYEVTRRLKSDGHLPFL
PILLVTASDRPSAVLGLDAGADEFIRKPIEPDELLARVRSLLRLKRSISERDNIARQREDFVARLAHDLRTPLVAADRMF
NLLGQGALGPMPPSMAETVDIMARSNSNLLALVRTLLDVYRYEAGAKKLHFQPVDVIELMRQVCDELAPLAAEKHLELCA
QLEAAPKVPADRLELRRVLTNLLGNALKFTDQGGVTVRSRLEDGGCVHLDFCDTGPGIAAEEQAYLFRRFAQGRHNKEGS
GMGLHLSRQIVEAHGGTLSLTSAPGKGSTFTVTLPAAK

Sequences:

>Translated_358_residues
MIQSAERILVVDDLPDNIALVQFLLASEGYEIETASSGAECLARVYKNSPDLLLLDVMMPGMDGYEVTRRLKSDGHLPFL
PILLVTASDRPSAVLGLDAGADEFIRKPIEPDELLARVRSLLRLKRSISERDNIARQREDFVARLAHDLRTPLVAADRMF
NLLGQGALGPMPPSMAETVDIMARSNSNLLALVRTLLDVYRYEAGAKKLHFQPVDVIELMRQVCDELAPLAAEKHLELCA
QLEAAPKVPADRLELRRVLTNLLGNALKFTDQGGVTVRSRLEDGGCVHLDFCDTGPGIAAEEQAYLFRRFAQGRHNKEGS
GMGLHLSRQIVEAHGGTLSLTSAPGKGSTFTVTLPAAK
>Mature_358_residues
MIQSAERILVVDDLPDNIALVQFLLASEGYEIETASSGAECLARVYKNSPDLLLLDVMMPGMDGYEVTRRLKSDGHLPFL
PILLVTASDRPSAVLGLDAGADEFIRKPIEPDELLARVRSLLRLKRSISERDNIARQREDFVARLAHDLRTPLVAADRMF
NLLGQGALGPMPPSMAETVDIMARSNSNLLALVRTLLDVYRYEAGAKKLHFQPVDVIELMRQVCDELAPLAAEKHLELCA
QLEAAPKVPADRLELRRVLTNLLGNALKFTDQGGVTVRSRLEDGGCVHLDFCDTGPGIAAEEQAYLFRRFAQGRHNKEGS
GMGLHLSRQIVEAHGGTLSLTSAPGKGSTFTVTLPAAK

Specific function: Member of the two-component regulatory system AlgB/KinB involved in regulation of alginate biosynthesis genes. KinB functions as a membrane-associated protein kinase that phosphorylates AlgB, probably in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788713, Length=228, Percent_Identity=32.8947368421053, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI145693157, Length=254, Percent_Identity=32.6771653543307, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI48994928, Length=250, Percent_Identity=31.2, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789149, Length=228, Percent_Identity=32.4561403508772, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87082128, Length=253, Percent_Identity=31.2252964426877, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI87081816, Length=243, Percent_Identity=31.2757201646091, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788393, Length=252, Percent_Identity=30.952380952381, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790436, Length=252, Percent_Identity=29.7619047619048, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1786912, Length=243, Percent_Identity=33.7448559670782, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786600, Length=245, Percent_Identity=28.9795918367347, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788279, Length=222, Percent_Identity=30.1801801801802, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789809, Length=143, Percent_Identity=35.6643356643357, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786783, Length=259, Percent_Identity=30.5019305019305, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790860, Length=132, Percent_Identity=34.8484848484849, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788394, Length=203, Percent_Identity=29.5566502463054, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786599, Length=120, Percent_Identity=35, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082012, Length=188, Percent_Identity=29.7872340425532, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787229, Length=131, Percent_Identity=36.6412213740458, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788549, Length=219, Percent_Identity=26.027397260274, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790861, Length=204, Percent_Identity=31.8627450980392, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786784, Length=190, Percent_Identity=29.4736842105263, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790299, Length=112, Percent_Identity=36.6071428571429, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790437, Length=122, Percent_Identity=36.8852459016393, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790863, Length=115, Percent_Identity=30.4347826086957, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788550, Length=121, Percent_Identity=34.7107438016529, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790300, Length=235, Percent_Identity=26.8085106382979, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF08448 PAS_4 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 39155; Mature: 39155

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQSAERILVVDDLPDNIALVQFLLASEGYEIETASSGAECLARVYKNSPDLLLLDVMMP
CCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCEEEEEECCC
GMDGYEVTRRLKSDGHLPFLPILLVTASDRPSAVLGLDAGADEFIRKPIEPDELLARVRS
CCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEECCCCCHHHHHCCCCHHHHHHHHHH
LLRLKRSISERDNIARQREDFVARLAHDLRTPLVAADRMFNLLGQGALGPMPPSMAETVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
IMARSNSNLLALVRTLLDVYRYEAGAKKLHFQPVDVIELMRQVCDELAPLAAEKHLELCA
HHHCCCCCHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLEAAPKVPADRLELRRVLTNLLGNALKFTDQGGVTVRSRLEDGGCVHLDFCDTGPGIAA
HHHCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCEEEEEECCCCCCCCC
EEQAYLFRRFAQGRHNKEGSGMGLHLSRQIVEAHGGTLSLTSAPGKGSTFTVTLPAAK
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCC
>Mature Secondary Structure
MIQSAERILVVDDLPDNIALVQFLLASEGYEIETASSGAECLARVYKNSPDLLLLDVMMP
CCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCEEEEEECCC
GMDGYEVTRRLKSDGHLPFLPILLVTASDRPSAVLGLDAGADEFIRKPIEPDELLARVRS
CCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEECCCCCHHHHHCCCCHHHHHHHHHH
LLRLKRSISERDNIARQREDFVARLAHDLRTPLVAADRMFNLLGQGALGPMPPSMAETVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
IMARSNSNLLALVRTLLDVYRYEAGAKKLHFQPVDVIELMRQVCDELAPLAAEKHLELCA
HHHCCCCCHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLEAAPKVPADRLELRRVLTNLLGNALKFTDQGGVTVRSRLEDGGCVHLDFCDTGPGIAA
HHHCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCEEEEEECCCCCCCCC
EEQAYLFRRFAQGRHNKEGSGMGLHLSRQIVEAHGGTLSLTSAPGKGSTFTVTLPAAK
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9218420 [H]