Click here to switch to the map view.

The map label for this gene is ruvB

Identifier: 37522688

GI number: 37522688

Start: 3320386

End: 3321516

Strand: Reverse

Name: ruvB

Synonym: gvip431

Alternate gene names: 37522688

Gene position: 3321516-3320386 (Counterclockwise)

Preceding gene: 37522691

Following gene: 37522687

Centisome position: 71.29

GC content: 65.87

Gene sequence:

>1131_bases
ATGGCGATCATCTCCTCCCGTCCTTCCGGAGCTGAGGGCGAACCGGCCAAATCGCGCACCGCCCCCGCCGAACCCGCCCA
ACGCTCCCGCACGGCGGTGCAGTCCGAGCGCGCCCACGAGGACCAGCACGAGGAGAGCCTCAGGCCCAAGAACCTTGTCG
ACTACGCCGGTCAAAAAGATCTCAAAGCCGTCCTGGGCATCGCCGTGGCCGCCGCCAAAAGTCGGGGCGAATCGCTCGAT
CACCTGCTGTTTTATGGACCGCCGGGACTGGGCAAGACGAGCATTTCGCTGATTCTGGCGCGCGAGATGAACGTGCAGAT
TCACCTCACCACCGCCCCTGCCCTCGAGCGTCCCCGCGACATCGCGGGTCTGCTCGTCAAACTCCGGCGCGGCGATATTT
TGTTCATCGACGAAATTCACCGCCTGCCCCGGCTCACCGAGGAGATTCTCTACCCGGCCATGGAGGACTATCGCCTCGAC
ATCACGATCGGCAAGGGACAAAACGCCCGGATTACCAGCGTGCCGCTGCCGCGCTTCACGCTCATCGGGGCCACCACCCG
CGTCGGTGCCCTCAGTTCGCCGCTGCGCGACCGCTTCGGCCTGATTCAGCGTTTGCGCTTTTACGATGTCGAAGAACTCG
CGGGGATCATTGTCCGCAACGCTGCCTTACTGAACATTTCGATCGATGAGCCTGGAGCAGCTGAGATCGCCCGCCGCGCC
CGGGGCACCCCGCGCATCGCCAACCGGCTGCTCAAGCGCGTGCGCGACTACGCCCAGGTCGAAGGGGACGGGCGGATCAC
CGAGCCGGTCGCCTGTGCGGCGCTGGAATTATTCGAAGTGGACCCGCGCGGGCTCGACTGGACCGATCGCCGCCTGCTTG
CCACCCTCATCGAGCACTACAACGGCGGCCCGGTGGGCGTCGAGTCCCTTGCCGCCGCCACCGGCGAAGACACCCAGACC
ATCGAAGAGGTCTACGAGCCCTATCTGATGCAAATCGGCTACCTGCTGCGCACCGCCCGCGGCCGGATGGCCTCCCCCGC
CGCCTACCGCCACTTGGGCTATGCGCCGCCCCAGCGCTTCGAGCAGGTCAGTCTGCTGGACAGCATTGACTCCGCGCACG
GGCGCAACTGA

Upstream 100 bases:

>100_bases
GTTGGCGGCGACCCACAGGAGCCCTGTGCGCCGTGGGCGCCGGGATGTGTTATCGTAATTGCGATCGGGACAAGCGTTCA
CCACTCGCGGCAGGTTGACC

Downstream 100 bases:

>100_bases
CCGGTCAAAATAGAAAGACCCTGAAGATCCCGGCGATGATCGATCCCAGCGAGGCCAAAGCCATTATTGAGAAGTACTTC
GCCGAGGTCACAGGCGAACA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MAIISSRPSGAEGEPAKSRTAPAEPAQRSRTAVQSERAHEDQHEESLRPKNLVDYAGQKDLKAVLGIAVAAAKSRGESLD
HLLFYGPPGLGKTSISLILAREMNVQIHLTTAPALERPRDIAGLLVKLRRGDILFIDEIHRLPRLTEEILYPAMEDYRLD
ITIGKGQNARITSVPLPRFTLIGATTRVGALSSPLRDRFGLIQRLRFYDVEELAGIIVRNAALLNISIDEPGAAEIARRA
RGTPRIANRLLKRVRDYAQVEGDGRITEPVACAALELFEVDPRGLDWTDRRLLATLIEHYNGGPVGVESLAAATGEDTQT
IEEVYEPYLMQIGYLLRTARGRMASPAAYRHLGYAPPQRFEQVSLLDSIDSAHGRN

Sequences:

>Translated_376_residues
MAIISSRPSGAEGEPAKSRTAPAEPAQRSRTAVQSERAHEDQHEESLRPKNLVDYAGQKDLKAVLGIAVAAAKSRGESLD
HLLFYGPPGLGKTSISLILAREMNVQIHLTTAPALERPRDIAGLLVKLRRGDILFIDEIHRLPRLTEEILYPAMEDYRLD
ITIGKGQNARITSVPLPRFTLIGATTRVGALSSPLRDRFGLIQRLRFYDVEELAGIIVRNAALLNISIDEPGAAEIARRA
RGTPRIANRLLKRVRDYAQVEGDGRITEPVACAALELFEVDPRGLDWTDRRLLATLIEHYNGGPVGVESLAAATGEDTQT
IEEVYEPYLMQIGYLLRTARGRMASPAAYRHLGYAPPQRFEQVSLLDSIDSAHGRN
>Mature_375_residues
AIISSRPSGAEGEPAKSRTAPAEPAQRSRTAVQSERAHEDQHEESLRPKNLVDYAGQKDLKAVLGIAVAAAKSRGESLDH
LLFYGPPGLGKTSISLILAREMNVQIHLTTAPALERPRDIAGLLVKLRRGDILFIDEIHRLPRLTEEILYPAMEDYRLDI
TIGKGQNARITSVPLPRFTLIGATTRVGALSSPLRDRFGLIQRLRFYDVEELAGIIVRNAALLNISIDEPGAAEIARRAR
GTPRIANRLLKRVRDYAQVEGDGRITEPVACAALELFEVDPRGLDWTDRRLLATLIEHYNGGPVGVESLAAATGEDTQTI
EEVYEPYLMQIGYLLRTARGRMASPAAYRHLGYAPPQRFEQVSLLDSIDSAHGRN

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=319, Percent_Identity=54.2319749216301, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1787119, Length=243, Percent_Identity=28.8065843621399, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324111, Length=232, Percent_Identity=28.8793103448276, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_GLOVI (Q7NGP9)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926065.1
- ProteinModelPortal:   Q7NGP9
- SMR:   Q7NGP9
- GeneID:   2601520
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.3119
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- BioCyc:   GVIO251221:GLL3119-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 41444; Mature: 41313

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIISSRPSGAEGEPAKSRTAPAEPAQRSRTAVQSERAHEDQHEESLRPKNLVDYAGQKD
CCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHH
LKAVLGIAVAAAKSRGESLDHLLFYGPPGLGKTSISLILAREMNVQIHLTTAPALERPRD
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHEEEEEEEECCEEEEEECCCHHCCCHH
IAGLLVKLRRGDILFIDEIHRLPRLTEEILYPAMEDYRLDITIGKGQNARITSVPLPRFT
HHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEECCCCCEE
LIGATTRVGALSSPLRDRFGLIQRLRFYDVEELAGIIVRNAALLNISIDEPGAAEIARRA
EEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEEECCCCCHHHHHHHH
RGTPRIANRLLKRVRDYAQVEGDGRITEPVACAALELFEVDPRGLDWTDRRLLATLIEHY
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
NGGPVGVESLAAATGEDTQTIEEVYEPYLMQIGYLLRTARGRMASPAAYRHLGYAPPQRF
CCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHH
EQVSLLDSIDSAHGRN
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIISSRPSGAEGEPAKSRTAPAEPAQRSRTAVQSERAHEDQHEESLRPKNLVDYAGQKD
CEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHH
LKAVLGIAVAAAKSRGESLDHLLFYGPPGLGKTSISLILAREMNVQIHLTTAPALERPRD
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHEEEEEEEECCEEEEEECCCHHCCCHH
IAGLLVKLRRGDILFIDEIHRLPRLTEEILYPAMEDYRLDITIGKGQNARITSVPLPRFT
HHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEECCCCCEE
LIGATTRVGALSSPLRDRFGLIQRLRFYDVEELAGIIVRNAALLNISIDEPGAAEIARRA
EEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEEECCCCCHHHHHHHH
RGTPRIANRLLKRVRDYAQVEGDGRITEPVACAALELFEVDPRGLDWTDRRLLATLIEHY
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
NGGPVGVESLAAATGEDTQTIEEVYEPYLMQIGYLLRTARGRMASPAAYRHLGYAPPQRF
CCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHH
EQVSLLDSIDSAHGRN
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292