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The map label for this gene is pbpE [H]

Identifier: 37522667

GI number: 37522667

Start: 3299105

End: 3300199

Strand: Reverse

Name: pbpE [H]

Synonym: gll3098

Alternate gene names: 37522667

Gene position: 3300199-3299105 (Counterclockwise)

Preceding gene: 37522668

Following gene: 37522666

Centisome position: 70.83

GC content: 60.37

Gene sequence:

>1095_bases
ATGAAAAAATGGCTGCCCGTTCTGCTGACATTGCTGCTGCTCTGCACAATGACCATTCCTGGCCGGGCCGATGCGATCGA
CGATTATGTCCAGAACCAGATGCGCGAGCAGCGCATTCCTGGTTTGTCGCTGGCGATCGTGCAGAACGACCGCCTCGTCA
AGGCCAAAGGGTACGGCCTGGCCAACGTCGAATTGAACGTCCCGGTCGCTCCCGAGAGTGTCTTTCAGTCGGGCTCGGTG
GGCAAGCAGTTCACGGCCACCGCGGTGATGCTGCTGGTGGAAGAAGGCAAAATCAATCTCGACGACAAGATCGGCCGTTA
TCTGGCGGGAGCGCCGTCCGCCTGGCGGGGGATCACCGTTTGTCATCTGCTCACCCACACCTCGGGCATCAGAGATTACG
AGGGCGAGAACGGACCGGCCATCGATTTTCGCCGCGACTACACCGACGAAGAACTGGTCGCCCGGGCCGCCGCCATGCCC
CTCGATTTTGCCCCGGGTGAAAAGTGGAAGTACAGCAACACCGGCTATGTACTACTGGGGATCATTGTCAAAAATGCCTC
GGGACAGTTCTATGGGGATTTGCTCGACGAACGGGTCTTCAAACCCCTCGGCATGCAGGCCACCCGGGTGATCACCGAGG
CGGACATCGTTGCCAACCGCGCCGCCGGCTACCGCCTGGAGAACGACGCCCTCAAAAATCAAGCGTATGTTTCCCCGTCG
CTCAATCGGACCGCCGACGGCTCGCTCTATCTGACGGTGCTCGACATGGTCAAGTGGGACGCAGCCCTCTACACCGAAAA
ACTACTCAAAAAGTCCAGCCTCGAGCGGATGTGGACGCCGGTGACGCTCAACGACGGCAAGACGTACCCTTATGGCTTTG
GATGGGCGCTCGGATCGGTGGCCGGGCGCCGCCTCATCGAGCACGCAGGGCAGTGGCAGGGATTTACCGCCCACATCGCC
CGCTACGTCGACGACAAACTCACCGTCATCGTCCTCACCAATCTGGCGCAAGCCAACCCGTCGCCCATTGCCCACGGGAT
CGCCGGATTGTACGTTCCAACTCTAGGACAACCTCAATCTTCCGCCGGCCGTTGA

Upstream 100 bases:

>100_bases
GCCTGGATCTAGCCCGCGACGTGGTGACGGGCGGTTTTGTGCTCAAAGACGGTTACCTATCCACCCGCGAAGCGCCCGGT
CTGGGCGTTCAATTATTGTC

Downstream 100 bases:

>100_bases
CGGATTTTTCGCGGCGACCCATGTAAAGTTGGGCATAGATGCAATGCCACGCAAGATTCATCGGATTGTTTGTCTATGAA
GTACTTTTTTTTGGCTGAAG

Product: penicillin-binding protein

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKKWLPVLLTLLLLCTMTIPGRADAIDDYVQNQMREQRIPGLSLAIVQNDRLVKAKGYGLANVELNVPVAPESVFQSGSV
GKQFTATAVMLLVEEGKINLDDKIGRYLAGAPSAWRGITVCHLLTHTSGIRDYEGENGPAIDFRRDYTDEELVARAAAMP
LDFAPGEKWKYSNTGYVLLGIIVKNASGQFYGDLLDERVFKPLGMQATRVITEADIVANRAAGYRLENDALKNQAYVSPS
LNRTADGSLYLTVLDMVKWDAALYTEKLLKKSSLERMWTPVTLNDGKTYPYGFGWALGSVAGRRLIEHAGQWQGFTAHIA
RYVDDKLTVIVLTNLAQANPSPIAHGIAGLYVPTLGQPQSSAGR

Sequences:

>Translated_364_residues
MKKWLPVLLTLLLLCTMTIPGRADAIDDYVQNQMREQRIPGLSLAIVQNDRLVKAKGYGLANVELNVPVAPESVFQSGSV
GKQFTATAVMLLVEEGKINLDDKIGRYLAGAPSAWRGITVCHLLTHTSGIRDYEGENGPAIDFRRDYTDEELVARAAAMP
LDFAPGEKWKYSNTGYVLLGIIVKNASGQFYGDLLDERVFKPLGMQATRVITEADIVANRAAGYRLENDALKNQAYVSPS
LNRTADGSLYLTVLDMVKWDAALYTEKLLKKSSLERMWTPVTLNDGKTYPYGFGWALGSVAGRRLIEHAGQWQGFTAHIA
RYVDDKLTVIVLTNLAQANPSPIAHGIAGLYVPTLGQPQSSAGR
>Mature_364_residues
MKKWLPVLLTLLLLCTMTIPGRADAIDDYVQNQMREQRIPGLSLAIVQNDRLVKAKGYGLANVELNVPVAPESVFQSGSV
GKQFTATAVMLLVEEGKINLDDKIGRYLAGAPSAWRGITVCHLLTHTSGIRDYEGENGPAIDFRRDYTDEELVARAAAMP
LDFAPGEKWKYSNTGYVLLGIIVKNASGQFYGDLLDERVFKPLGMQATRVITEADIVANRAAGYRLENDALKNQAYVSPS
LNRTADGSLYLTVLDMVKWDAALYTEKLLKKSSLERMWTPVTLNDGKTYPYGFGWALGSVAGRRLIEHAGQWQGFTAHIA
RYVDDKLTVIVLTNLAQANPSPIAHGIAGLYVPTLGQPQSSAGR

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI1790593, Length=261, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786574, Length=328, Percent_Identity=27.1341463414634, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082101, Length=206, Percent_Identity=26.2135922330097, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17550934, Length=325, Percent_Identity=28.9230769230769, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI115533026, Length=372, Percent_Identity=26.3440860215054, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17565630, Length=196, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 39932; Mature: 39932

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWLPVLLTLLLLCTMTIPGRADAIDDYVQNQMREQRIPGLSLAIVQNDRLVKAKGYGL
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEECCCEE
ANVELNVPVAPESVFQSGSVGKQFTATAVMLLVEEGKINLDDKIGRYLAGAPSAWRGITV
EEEEEECCCCCHHHHCCCCCCCHHHHHEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHH
CHLLTHTSGIRDYEGENGPAIDFRRDYTDEELVARAAAMPLDFAPGEKWKYSNTGYVLLG
HHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEE
IIVKNASGQFYGDLLDERVFKPLGMQATRVITEADIVANRAAGYRLENDALKNQAYVSPS
EEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEECCHHHCCCEEECCC
LNRTADGSLYLTVLDMVKWDAALYTEKLLKKSSLERMWTPVTLNDGKTYPYGFGWALGSV
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHH
AGRRLIEHAGQWQGFTAHIARYVDDKLTVIVLTNLAQANPSPIAHGIAGLYVPTLGQPQS
HHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEECHHCCCCCHHHHHHHHHCCCCCCCCCC
SAGR
CCCC
>Mature Secondary Structure
MKKWLPVLLTLLLLCTMTIPGRADAIDDYVQNQMREQRIPGLSLAIVQNDRLVKAKGYGL
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEECCCEE
ANVELNVPVAPESVFQSGSVGKQFTATAVMLLVEEGKINLDDKIGRYLAGAPSAWRGITV
EEEEEECCCCCHHHHCCCCCCCHHHHHEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHH
CHLLTHTSGIRDYEGENGPAIDFRRDYTDEELVARAAAMPLDFAPGEKWKYSNTGYVLLG
HHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEE
IIVKNASGQFYGDLLDERVFKPLGMQATRVITEADIVANRAAGYRLENDALKNQAYVSPS
EEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEECCHHHCCCEEECCC
LNRTADGSLYLTVLDMVKWDAALYTEKLLKKSSLERMWTPVTLNDGKTYPYGFGWALGSV
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHH
AGRRLIEHAGQWQGFTAHIARYVDDKLTVIVLTNLAQANPSPIAHGIAGLYVPTLGQPQS
HHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEECHHCCCCCHHHHHHHHHCCCCCCCCCC
SAGR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]