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The map label for this gene is glcF [H]

Identifier: 37522641

GI number: 37522641

Start: 3268399

End: 3269745

Strand: Direct

Name: glcF [H]

Synonym: gvip422

Alternate gene names: 37522641

Gene position: 3268399-3269745 (Clockwise)

Preceding gene: 37522640

Following gene: 37522642

Centisome position: 70.15

GC content: 65.63

Gene sequence:

>1347_bases
ATGGAGAACCAGCCCCGGCCCACCGTCCCCAGAGCCTTCGATGCGGACCACCCCCCCGATGCGCAGATCATCGACACCTG
CGTACACTGCGGCTTTTGCCTTGCTACCTGTCCGAGCTACCGGGTGCTCGGCAAGGAGATGGACTCGCCCCGGGGCCGCA
TTTACTTGATGGACGCGCTCAACGAGGGACAGATTCCCCTCTCCCAGACAACCGCGGGCCATTTCGACAGCTGCCTGGGC
TGCCTCGCCTGTGTCAGCACCTGTCCCTCCGGCGTGCAGTACGACCAGCTCATCACCGCCACCCGCCCCCAGGTGGAACG
CAACGTCGAGCGCTCCGTGCCCGACCGGCTGCTGCGCGCCTTGATCTTTAACCTGCTGCCGTACCCGCGCCGGTTGCGCC
CGCTGCTCGTACCGCTGGCGATCTATCAGAAACTCGGAGTGCAGAAGCTGGTGCGCAAAAGCGGCCTGCTGCAAAAAGTC
GTCCCCCGCCTTGCCGCGATGGAGTCGATCCTTCCTGAAGTGCCCGCGAGCGCCTTTGCGGCCCCGATGCCCGAGCGGGC
GGCCGCCCGGGGCAAGCAGCGCTTTCGGGTGGGACTGATCACCGGCTGCGTGCAGCGGCTATTTTTTGACCGGGTCAACC
GCGCCACGCTGCGGGTACTCACCGCCATCGGCTGCGAGGTGGTCATCCCCCGCTCCCAGGGCTGCTGCGCAGCCCTTCCC
CAGCACCAGGGCCAGAGTGGGCAGGCCCGGCAAATGGCCCGCGACCTCATCGACAGTTTTGCCGACAGCAATGTCGATTA
TCTGATCATCAACGCCGCCGGCTGCGGCCACACTCTCAAGGAGTACGGCCACATCCTGGCGGAGGACCCTGTCTACCGCG
AGCGGGCCGCCGCCTTTGCCGCGAAAGTGCGCGACGTGCAGGAATTTTTGTACTGGGCCAAGATCCCCGTCCCCCTCCAA
CCGATTGCCGACGGGCCGCTTGCGGTCGTCTATCAGGACGCCTGCCACCTGCTGCACGGCCAGCGCATCAGCCTGCAGCC
GCGCCAGTTGCTTGCTCAGATTCCTGGAGTGCAAGTGCGCGAACCGGTGGATGCGGCCCTCTGCTGCGGCAGCGCCGGGG
TCTACAACATTCTGCAGCCGGAGATCGCCGAGGAACTCGGCCGTCAAAAAGTCGAGAATCTGCTCGCGACGGGTGCCGTG
CTCATCGCTTCTGCCAACCCCGGCTGCGCGCTGCAGATCCGCAAGCACCTGGAATTGACCGCTCAGGCAGTACCCGTGAG
GCATCCCATGGAACTCATCGACGCCGCCATTCGCGGGGTGAAAGTTGCGGATCTTCCCGCAGGCTGA

Upstream 100 bases:

>100_bases
ACAGATTTTTGCCTGCCGCCCCTTCGGGTCGGGCTGATCCCGGCCGGGCACACCGTCCGGGTGTACCTTGCAAGCAGGCG
CGATCCCAGCGAGTTGACCC

Downstream 100 bases:

>100_bases
GGCCGATCCCCGCGACAAAAGCCGGTAAATTCTCGCTGTACTATGAGGAGACGCGCACCAGCCGCTCTCCAGTCAAGTTT
CGAGGATCTCCGATGGTCGT

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MENQPRPTVPRAFDADHPPDAQIIDTCVHCGFCLATCPSYRVLGKEMDSPRGRIYLMDALNEGQIPLSQTTAGHFDSCLG
CLACVSTCPSGVQYDQLITATRPQVERNVERSVPDRLLRALIFNLLPYPRRLRPLLVPLAIYQKLGVQKLVRKSGLLQKV
VPRLAAMESILPEVPASAFAAPMPERAAARGKQRFRVGLITGCVQRLFFDRVNRATLRVLTAIGCEVVIPRSQGCCAALP
QHQGQSGQARQMARDLIDSFADSNVDYLIINAAGCGHTLKEYGHILAEDPVYRERAAAFAAKVRDVQEFLYWAKIPVPLQ
PIADGPLAVVYQDACHLLHGQRISLQPRQLLAQIPGVQVREPVDAALCCGSAGVYNILQPEIAEELGRQKVENLLATGAV
LIASANPGCALQIRKHLELTAQAVPVRHPMELIDAAIRGVKVADLPAG

Sequences:

>Translated_448_residues
MENQPRPTVPRAFDADHPPDAQIIDTCVHCGFCLATCPSYRVLGKEMDSPRGRIYLMDALNEGQIPLSQTTAGHFDSCLG
CLACVSTCPSGVQYDQLITATRPQVERNVERSVPDRLLRALIFNLLPYPRRLRPLLVPLAIYQKLGVQKLVRKSGLLQKV
VPRLAAMESILPEVPASAFAAPMPERAAARGKQRFRVGLITGCVQRLFFDRVNRATLRVLTAIGCEVVIPRSQGCCAALP
QHQGQSGQARQMARDLIDSFADSNVDYLIINAAGCGHTLKEYGHILAEDPVYRERAAAFAAKVRDVQEFLYWAKIPVPLQ
PIADGPLAVVYQDACHLLHGQRISLQPRQLLAQIPGVQVREPVDAALCCGSAGVYNILQPEIAEELGRQKVENLLATGAV
LIASANPGCALQIRKHLELTAQAVPVRHPMELIDAAIRGVKVADLPAG
>Mature_448_residues
MENQPRPTVPRAFDADHPPDAQIIDTCVHCGFCLATCPSYRVLGKEMDSPRGRIYLMDALNEGQIPLSQTTAGHFDSCLG
CLACVSTCPSGVQYDQLITATRPQVERNVERSVPDRLLRALIFNLLPYPRRLRPLLVPLAIYQKLGVQKLVRKSGLLQKV
VPRLAAMESILPEVPASAFAAPMPERAAARGKQRFRVGLITGCVQRLFFDRVNRATLRVLTAIGCEVVIPRSQGCCAALP
QHQGQSGQARQMARDLIDSFADSNVDYLIINAAGCGHTLKEYGHILAEDPVYRERAAAFAAKVRDVQEFLYWAKIPVPLQ
PIADGPLAVVYQDACHLLHGQRISLQPRQLLAQIPGVQVREPVDAALCCGSAGVYNILQPEIAEELGRQKVENLLATGAV
LIASANPGCALQIRKHLELTAQAVPVRHPMELIDAAIRGVKVADLPAG

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=431, Percent_Identity=32.7146171693735, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1786497, Length=239, Percent_Identity=27.1966527196653, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 48863; Mature: 48863

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENQPRPTVPRAFDADHPPDAQIIDTCVHCGFCLATCPSYRVLGKEMDSPRGRIYLMDAL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEECC
NEGQIPLSQTTAGHFDSCLGCLACVSTCPSGVQYDQLITATRPQVERNVERSVPDRLLRA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
LIFNLLPYPRRLRPLLVPLAIYQKLGVQKLVRKSGLLQKVVPRLAAMESILPEVPASAFA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHC
APMPERAAARGKQRFRVGLITGCVQRLFFDRVNRATLRVLTAIGCEVVIPRSQGCCAALP
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHCC
QHQGQSGQARQMARDLIDSFADSNVDYLIINAAGCGHTLKEYGHILAEDPVYRERAAAFA
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCHHHCCCCHHHHHHHHHH
AKVRDVQEFLYWAKIPVPLQPIADGPLAVVYQDACHLLHGQRISLQPRQLLAQIPGVQVR
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCC
EPVDAALCCGSAGVYNILQPEIAEELGRQKVENLLATGAVLIASANPGCALQIRKHLELT
CCHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHH
AQAVPVRHPMELIDAAIRGVKVADLPAG
HHHCCCCCHHHHHHHHHCCCEEECCCCC
>Mature Secondary Structure
MENQPRPTVPRAFDADHPPDAQIIDTCVHCGFCLATCPSYRVLGKEMDSPRGRIYLMDAL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEECC
NEGQIPLSQTTAGHFDSCLGCLACVSTCPSGVQYDQLITATRPQVERNVERSVPDRLLRA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
LIFNLLPYPRRLRPLLVPLAIYQKLGVQKLVRKSGLLQKVVPRLAAMESILPEVPASAFA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHC
APMPERAAARGKQRFRVGLITGCVQRLFFDRVNRATLRVLTAIGCEVVIPRSQGCCAALP
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHCC
QHQGQSGQARQMARDLIDSFADSNVDYLIINAAGCGHTLKEYGHILAEDPVYRERAAAFA
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCHHHCCCCHHHHHHHHHH
AKVRDVQEFLYWAKIPVPLQPIADGPLAVVYQDACHLLHGQRISLQPRQLLAQIPGVQVR
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCC
EPVDAALCCGSAGVYNILQPEIAEELGRQKVENLLATGAVLIASANPGCALQIRKHLELT
CCHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHH
AQAVPVRHPMELIDAAIRGVKVADLPAG
HHHCCCCCHHHHHHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]