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The map label for this gene is lpdA [H]

Identifier: 37522598

GI number: 37522598

Start: 3226534

End: 3227913

Strand: Direct

Name: lpdA [H]

Synonym: glr3029

Alternate gene names: 37522598

Gene position: 3226534-3227913 (Clockwise)

Preceding gene: 37522596

Following gene: 37522599

Centisome position: 69.25

GC content: 64.93

Gene sequence:

>1380_bases
GTGGCATTCGACTACGACTTGCTGATCATCGGCTGTGGCGTCGGCGGGCACGGGGCGGCCCTGCACGCGGTGGCCCACAA
GCTCAAAGTGGCTGTGGTCGAGGCCCGCGACCTCGGGGGCACCTGCATCAACCGCGGCTGCATCCCTTCAAAAGCGTTGC
TCGCCGCCGCCGGCCGGCTCCGGGTGCTGCGCGAGAGCGAACACCTGGGAATCAGCGTCGGCGAAATCAGCTTCGACCGT
GCCAAGATTGCCCACCACGCCGCGAGCGTCGTCGATAAAATCCGCGCGGATCTGACCAAGAGCCTTCAGAAACTGGGGGT
AACCATCCTGCACGGCCATGCCCGCCTCAAAGGTTCCCAGACGGTCGAAATCGACCCGGGCGAAGGCGGAGGCGAAGTCC
AGGTGCTCACCGCCCGCGACGTACTCATCGCGAGCGGCTCACAGCCTTTTGTGCCGCCGGGGATTGTCACCGACGGCAAG
ACCGTTTACACCAGCGACGAGGGCGTGCGCCTCGAACACCTCCCCGAGCACATCTGCATCATCGGCTCAGGCTACATCGG
CCTCGAATTTTCGGACGTCTACACAGCCCTGGGGACCAAAGTGACGATGATCGAAGCGCTCGATCGGCTTATGCCGGGTT
TCGATCCGGATATCGCCCGCCTTGCCGCCCGCCTGCTCGTCAAATCCCGCGACATCGACACCAAAGTCGGCGTCTTCGCC
AAAAAAGTCACCCCCGGCCAACCGGCGACGGTGGAACTCTCGACCGGCGAGCAACTCCAGTTCGACGCGGTGCTGGTGGC
CTGCGGCCGGACTCCCGATACCAAGAACCTGGGTCTGGAGTCCGTGGGTCTCGAGACGGCCAGGGGCTTTATCCCGGTGG
ACGGACGCATGGCCACCTCCGCCGAGCACCTCTGGGCAATCGGAGACGCAACCGGCAAGATGATGCTCGCCCACGCCGCC
TCCGCCCAGGGGATCGTCGCGGTCGACAACATGCTGGGTCACGAGCGCACGATCGACTACCGCTCGATCCCGGCCGCCTG
CTTCACCCACCCGGAAATCGGCTTTGTGGGACTCACCGAACCCCAGGCCAAAGAGCAGGGTTATAAAGTTGGGGTGGTAC
GGACGTACTTTGGCGGGAATTCTAAGGCCATCGCCCAGGGGGACACCGAGGGGATGGCCAAAATCGTCTACGACCAGACC
ACCGGCGAACTGCTCGGTTGCCACATCATCGGCCCCGAGGCGTCGCTGTTGGTGGCGGAGGCGGCCCAGGCCATCGCCGG
TCGCGACCGCATCGAGCGGCTCGCGCACCTGGTGCACACCCACCCCACCCTCAGCGAGATTCTCGACGAAGGGTACAAAC
GGGCAGCCGCCGTCCTGTAG

Upstream 100 bases:

>100_bases
ATCGAATCAATACCGGCAAAAAAGATCCCTATTCACCCCGGCAGCGGCCGTTGGACATCCTGGCGTAGGATAAATTCACG
TTTGCATTGGGGGATGGACC

Downstream 100 bases:

>100_bases
GAATCTGCCGTTCCATGAGCACTCTCGATCTCGCCGTTCCGAGTACCCGTTACTTACAAAAGCTCATCCGCGACCAGTCG
CCCGTCTCGATCGCCACTCG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; LPD; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MAFDYDLLIIGCGVGGHGAALHAVAHKLKVAVVEARDLGGTCINRGCIPSKALLAAAGRLRVLRESEHLGISVGEISFDR
AKIAHHAASVVDKIRADLTKSLQKLGVTILHGHARLKGSQTVEIDPGEGGGEVQVLTARDVLIASGSQPFVPPGIVTDGK
TVYTSDEGVRLEHLPEHICIIGSGYIGLEFSDVYTALGTKVTMIEALDRLMPGFDPDIARLAARLLVKSRDIDTKVGVFA
KKVTPGQPATVELSTGEQLQFDAVLVACGRTPDTKNLGLESVGLETARGFIPVDGRMATSAEHLWAIGDATGKMMLAHAA
SAQGIVAVDNMLGHERTIDYRSIPAACFTHPEIGFVGLTEPQAKEQGYKVGVVRTYFGGNSKAIAQGDTEGMAKIVYDQT
TGELLGCHIIGPEASLLVAEAAQAIAGRDRIERLAHLVHTHPTLSEILDEGYKRAAAVL

Sequences:

>Translated_459_residues
MAFDYDLLIIGCGVGGHGAALHAVAHKLKVAVVEARDLGGTCINRGCIPSKALLAAAGRLRVLRESEHLGISVGEISFDR
AKIAHHAASVVDKIRADLTKSLQKLGVTILHGHARLKGSQTVEIDPGEGGGEVQVLTARDVLIASGSQPFVPPGIVTDGK
TVYTSDEGVRLEHLPEHICIIGSGYIGLEFSDVYTALGTKVTMIEALDRLMPGFDPDIARLAARLLVKSRDIDTKVGVFA
KKVTPGQPATVELSTGEQLQFDAVLVACGRTPDTKNLGLESVGLETARGFIPVDGRMATSAEHLWAIGDATGKMMLAHAA
SAQGIVAVDNMLGHERTIDYRSIPAACFTHPEIGFVGLTEPQAKEQGYKVGVVRTYFGGNSKAIAQGDTEGMAKIVYDQT
TGELLGCHIIGPEASLLVAEAAQAIAGRDRIERLAHLVHTHPTLSEILDEGYKRAAAVL
>Mature_458_residues
AFDYDLLIIGCGVGGHGAALHAVAHKLKVAVVEARDLGGTCINRGCIPSKALLAAAGRLRVLRESEHLGISVGEISFDRA
KIAHHAASVVDKIRADLTKSLQKLGVTILHGHARLKGSQTVEIDPGEGGGEVQVLTARDVLIASGSQPFVPPGIVTDGKT
VYTSDEGVRLEHLPEHICIIGSGYIGLEFSDVYTALGTKVTMIEALDRLMPGFDPDIARLAARLLVKSRDIDTKVGVFAK
KVTPGQPATVELSTGEQLQFDAVLVACGRTPDTKNLGLESVGLETARGFIPVDGRMATSAEHLWAIGDATGKMMLAHAAS
AQGIVAVDNMLGHERTIDYRSIPAACFTHPEIGFVGLTEPQAKEQGYKVGVVRTYFGGNSKAIAQGDTEGMAKIVYDQTT
GELLGCHIIGPEASLLVAEAAQAIAGRDRIERLAHLVHTHPTLSEILDEGYKRAAAVL

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=459, Percent_Identity=36.1655773420479, Blast_Score=269, Evalue=5e-72,
Organism=Homo sapiens, GI50301238, Length=465, Percent_Identity=29.6774193548387, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI22035672, Length=471, Percent_Identity=30.5732484076433, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI33519430, Length=430, Percent_Identity=26.5116279069767, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI33519428, Length=430, Percent_Identity=26.5116279069767, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI33519426, Length=430, Percent_Identity=26.5116279069767, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI148277071, Length=430, Percent_Identity=26.7441860465116, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI148277065, Length=430, Percent_Identity=26.5116279069767, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI291045266, Length=482, Percent_Identity=26.1410788381743, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI291045268, Length=337, Percent_Identity=26.7062314540059, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786307, Length=449, Percent_Identity=34.075723830735, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI87082354, Length=454, Percent_Identity=31.7180616740088, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI87081717, Length=459, Percent_Identity=29.1938997821351, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789915, Length=438, Percent_Identity=29.6803652968037, Blast_Score=169, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI32565766, Length=463, Percent_Identity=36.0691144708423, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI71983429, Length=345, Percent_Identity=28.695652173913, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71983419, Length=369, Percent_Identity=29.2682926829268, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17557007, Length=473, Percent_Identity=26.4270613107822, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=444, Percent_Identity=25.9009009009009, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321091, Length=466, Percent_Identity=34.549356223176, Blast_Score=230, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6325240, Length=476, Percent_Identity=27.7310924369748, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6325166, Length=461, Percent_Identity=26.6811279826464, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21358499, Length=471, Percent_Identity=36.7303609341826, Blast_Score=265, Evalue=7e-71,
Organism=Drosophila melanogaster, GI24640553, Length=486, Percent_Identity=28.1893004115226, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24640549, Length=486, Percent_Identity=28.1893004115226, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24640551, Length=486, Percent_Identity=28.1893004115226, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17737741, Length=473, Percent_Identity=27.061310782241, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48498; Mature: 48367

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFDYDLLIIGCGVGGHGAALHAVAHKLKVAVVEARDLGGTCINRGCIPSKALLAAAGRL
CCEEEEEEEEEECCCCCHHHHHHHHHHHEEEEEEECCCCCHHHCCCCCCHHHHHHHHHHH
RVLRESEHLGISVGEISFDRAKIAHHAASVVDKIRADLTKSLQKLGVTILHGHARLKGSQ
EEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEECCCC
TVEIDPGEGGGEVQVLTARDVLIASGSQPFVPPGIVTDGKTVYTSDEGVRLEHLPEHICI
EEEECCCCCCCEEEEEEECEEEEECCCCCCCCCCEEECCCEEEECCCCCCHHCCCCEEEE
IGSGYIGLEFSDVYTALGTKVTMIEALDRLMPGFDPDIARLAARLLVKSRDIDTKVGVFA
EECCEEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHCEEE
KKVTPGQPATVELSTGEQLQFDAVLVACGRTPDTKNLGLESVGLETARGFIPVDGRMATS
EECCCCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCCHHCCHHHHCCEEECCCCEECC
AEHLWAIGDATGKMMLAHAASAQGIVAVDNMLGHERTIDYRSIPAACFTHPEIGFVGLTE
CCCEEEEECCCCCEEEEECCCCCCEEEEHHHCCCCCCCCCCCCCHHHCCCCCCCEEECCC
PQAKEQGYKVGVVRTYFGGNSKAIAQGDTEGMAKIVYDQTTGELLGCHIIGPEASLLVAE
CHHHHCCEEEEEEEEEECCCCCEEECCCCCCCEEEEEECCCCCEEEEEEECCCHHHHHHH
AAQAIAGRDRIERLAHLVHTHPTLSEILDEGYKRAAAVL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AFDYDLLIIGCGVGGHGAALHAVAHKLKVAVVEARDLGGTCINRGCIPSKALLAAAGRL
CEEEEEEEEEECCCCCHHHHHHHHHHHEEEEEEECCCCCHHHCCCCCCHHHHHHHHHHH
RVLRESEHLGISVGEISFDRAKIAHHAASVVDKIRADLTKSLQKLGVTILHGHARLKGSQ
EEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEECCCC
TVEIDPGEGGGEVQVLTARDVLIASGSQPFVPPGIVTDGKTVYTSDEGVRLEHLPEHICI
EEEECCCCCCCEEEEEEECEEEEECCCCCCCCCCEEECCCEEEECCCCCCHHCCCCEEEE
IGSGYIGLEFSDVYTALGTKVTMIEALDRLMPGFDPDIARLAARLLVKSRDIDTKVGVFA
EECCEEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHCEEE
KKVTPGQPATVELSTGEQLQFDAVLVACGRTPDTKNLGLESVGLETARGFIPVDGRMATS
EECCCCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCCHHCCHHHHCCEEECCCCEECC
AEHLWAIGDATGKMMLAHAASAQGIVAVDNMLGHERTIDYRSIPAACFTHPEIGFVGLTE
CCCEEEEECCCCCEEEEECCCCCCEEEEHHHCCCCCCCCCCCCCHHHCCCCCCCEEECCC
PQAKEQGYKVGVVRTYFGGNSKAIAQGDTEGMAKIVYDQTTGELLGCHIIGPEASLLVAE
CHHHHCCEEEEEEEEEECCCCCEEECCCCCCCEEEEEECCCCCEEEEEEECCCHHHHHHH
AAQAIAGRDRIERLAHLVHTHPTLSEILDEGYKRAAAVL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231; 9387233 [H]