Definition Gloeobacter violaceus PCC 7421 chromosome, complete genome.
Accession NC_005125
Length 4,659,019

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The map label for this gene is pyrB

Identifier: 37522444

GI number: 37522444

Start: 3069544

End: 3070533

Strand: Reverse

Name: pyrB

Synonym: gvip392

Alternate gene names: 37522444

Gene position: 3070533-3069544 (Counterclockwise)

Preceding gene: 37522446

Following gene: 37522443

Centisome position: 65.91

GC content: 64.85

Gene sequence:

>990_bases
GTGCTCGCCGCTTTGAATGCGCCGTTTCCCTGGACCCGCCGCCACGTTTTGAGCCTGGAGGATTTCAGCGCCGCCGAGTA
CGCCCTGGTATTGCAGACAGCCGGTAGCTTTCAGCAGGTGCTCGCCCGGCGCAACCGCAAAGTTCCGACGCTGCAGGGCC
GGATTATCGTCAATTTGTTCTTCGAGTCCTCGACGCGCACCCGCACGAGCTTTGAACTGGCGGCCAAGGCCCTCTCGGCG
GATGTGGTCAACTTCTCCGCCGCTACCTCCTCGCTCACCAAGGGCGAAACCATCTTCGACACCACCCGCACCTTTCTGGC
GATGGGCATGGATATCGTCGTCGTCCGCCACCGAGACAGCGGCGTACCCCATGCCGTCGCCCGCGACCTCGAACAACTCG
GTAGCCCCGTGAGCGTGATCAATGCGGGCGACGGCCAGCACGAGCACCCCACCCAGGCGCTGCTTGACCTGTTTACCCTC
TGCACACTGCTGGATGCCTACGATCCTCGCCCCGAGTTGCTCGCGGGCAAAAAAATCGTCATCGTCGGCGATATTCTTCA
CTCGCGCGTCGCCCGCTCGAATCTGTGCAGTCTGGTCACCTGCGGGGGCGAGGTGCACCTGGCCGGGCCGCCGACGCTGT
TGCCCGGCGAATTTCGCCAGTACGGCGCCACCCTCCACCACCACCTCGCCCCCGCCCTCGAAGGGGCGGATTTTGTGATG
ACCCTGCGCCTGCAAAAAGAACGCATGGGCGATTATCTGCTGCCCAGTCTGCGCGAGTACCACCGCCTCTACGGGATCAC
CCGCGAGCGGCTCGCCCTGTGCAGTCCGGGGGTGCGCCTGCTTCACCCCGGCCCGGTCAACCGCGGCGTCGAACTGAGTT
CCGATTTGCTCGACGATCCGCGCCTCAGCCTGGTTTCCCAGCAGGTCACCAGCGGTGTGGCGGTGCGCATGGCCCTGCTC
TATCTGCTTGCTGGGGGCAAGGCGGCTTGA

Upstream 100 bases:

>100_bases
CTTCGATCCTATTCAACCTGGCAGCTGTCTCCATGCCTCCCACGGCATAGGCTGGCGGGCCGAATCGCGGAGCGCGGCGG
TATACTGAGCGAGCCGATTG

Downstream 100 bases:

>100_bases
AGATTTCCTTGCGGGGGGACCGCTGCAACGGCAACACTGGAGAGAGGTGGAGTTAAGTAGACAATGACCGAAAAAACGGC
AGGCCGCCACCCGCGCGACT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MLAALNAPFPWTRRHVLSLEDFSAAEYALVLQTAGSFQQVLARRNRKVPTLQGRIIVNLFFESSTRTRTSFELAAKALSA
DVVNFSAATSSLTKGETIFDTTRTFLAMGMDIVVVRHRDSGVPHAVARDLEQLGSPVSVINAGDGQHEHPTQALLDLFTL
CTLLDAYDPRPELLAGKKIVIVGDILHSRVARSNLCSLVTCGGEVHLAGPPTLLPGEFRQYGATLHHHLAPALEGADFVM
TLRLQKERMGDYLLPSLREYHRLYGITRERLALCSPGVRLLHPGPVNRGVELSSDLLDDPRLSLVSQQVTSGVAVRMALL
YLLAGGKAA

Sequences:

>Translated_329_residues
MLAALNAPFPWTRRHVLSLEDFSAAEYALVLQTAGSFQQVLARRNRKVPTLQGRIIVNLFFESSTRTRTSFELAAKALSA
DVVNFSAATSSLTKGETIFDTTRTFLAMGMDIVVVRHRDSGVPHAVARDLEQLGSPVSVINAGDGQHEHPTQALLDLFTL
CTLLDAYDPRPELLAGKKIVIVGDILHSRVARSNLCSLVTCGGEVHLAGPPTLLPGEFRQYGATLHHHLAPALEGADFVM
TLRLQKERMGDYLLPSLREYHRLYGITRERLALCSPGVRLLHPGPVNRGVELSSDLLDDPRLSLVSQQVTSGVAVRMALL
YLLAGGKAA
>Mature_329_residues
MLAALNAPFPWTRRHVLSLEDFSAAEYALVLQTAGSFQQVLARRNRKVPTLQGRIIVNLFFESSTRTRTSFELAAKALSA
DVVNFSAATSSLTKGETIFDTTRTFLAMGMDIVVVRHRDSGVPHAVARDLEQLGSPVSVINAGDGQHEHPTQALLDLFTL
CTLLDAYDPRPELLAGKKIVIVGDILHSRVARSNLCSLVTCGGEVHLAGPPTLLPGEFRQYGATLHHHLAPALEGADFVM
TLRLQKERMGDYLLPSLREYHRLYGITRERLALCSPGVRLLHPGPVNRGVELSSDLLDDPRLSLVSQQVTSGVAVRMALL
YLLAGGKAA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=324, Percent_Identity=32.4074074074074, Blast_Score=145, Evalue=6e-35,
Organism=Escherichia coli, GI2367364, Length=327, Percent_Identity=31.1926605504587, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1786469, Length=295, Percent_Identity=28.135593220339, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790703, Length=294, Percent_Identity=29.9319727891156, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=342, Percent_Identity=29.8245614035088, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322331, Length=330, Percent_Identity=33.3333333333333, Blast_Score=136, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24642586, Length=327, Percent_Identity=30.5810397553517, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_GLOVI (Q7NCV1)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925821.1
- ProteinModelPortal:   Q7NCV1
- SMR:   Q7NCV1
- GeneID:   2601276
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.2875
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   GVIO251221:GLL2875-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 35861; Mature: 35861

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MLAALNAPFPWTRRHVLSLEDFSAAEYALVLQTAGSFQQVLARRNRKVPTLQGRIIVNLF
CCCCCCCCCCCCHHHEECCCCCCCHHEEEEECCCCHHHHHHHHCCCCCCCCCCEEEEEEE
FESSTRTRTSFELAAKALSADVVNFSAATSSLTKGETIFDTTRTFLAMGMDIVVVRHRDS
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCC
GVPHAVARDLEQLGSPVSVINAGDGQHEHPTQALLDLFTLCTLLDAYDPRPELLAGKKIV
CCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEE
IVGDILHSRVARSNLCSLVTCGGEVHLAGPPTLLPGEFRQYGATLHHHLAPALEGADFVM
EEHHHHHHHHHHHHCCEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
TLRLQKERMGDYLLPSLREYHRLYGITRERLALCSPGVRLLHPGPVNRGVELSSDLLDDP
EEEECHHHCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCCCCCHHHCCCH
RLSLVSQQVTSGVAVRMALLYLLAGGKAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLAALNAPFPWTRRHVLSLEDFSAAEYALVLQTAGSFQQVLARRNRKVPTLQGRIIVNLF
CCCCCCCCCCCCHHHEECCCCCCCHHEEEEECCCCHHHHHHHHCCCCCCCCCCEEEEEEE
FESSTRTRTSFELAAKALSADVVNFSAATSSLTKGETIFDTTRTFLAMGMDIVVVRHRDS
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCC
GVPHAVARDLEQLGSPVSVINAGDGQHEHPTQALLDLFTLCTLLDAYDPRPELLAGKKIV
CCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEE
IVGDILHSRVARSNLCSLVTCGGEVHLAGPPTLLPGEFRQYGATLHHHLAPALEGADFVM
EEHHHHHHHHHHHHCCEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
TLRLQKERMGDYLLPSLREYHRLYGITRERLALCSPGVRLLHPGPVNRGVELSSDLLDDP
EEEECHHHCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCCCCCHHHCCCH
RLSLVSQQVTSGVAVRMALLYLLAGGKAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292