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The map label for this gene is ispD

Identifier: 37522360

GI number: 37522360

Start: 2970495

End: 2971196

Strand: Direct

Name: ispD

Synonym: glr2791

Alternate gene names: 37522360

Gene position: 2970495-2971196 (Clockwise)

Preceding gene: 37522359

Following gene: 37522361

Centisome position: 63.76

GC content: 65.81

Gene sequence:

>702_bases
ATGCACCTTTTGATTCCCGCCGCCGGGCGCGGTAGCCGCATGGGCGCAGACGGCAACAAGCTGCTCTTGCCGCTGTCGGG
CCGGCCGCTTCTGGCCTGGACGCTCGCAGCAGCGGCACAAGCCCCTTCAACCCGCTGGATAGGCATCATCGGCCAGCCGA
TGGACGAAGAAGCGATCCGGTTACTGGCGCAGAAGCTGGGTCTGCGGGTGCCGGTGCACTGGATCGAAGGGGGCGAGACC
CGTCAGCAGTCGGTTTACCGGGGGCTGCTCGCGCTTCCTGTAGAAGCGCAGCAGGTACTCATCCACGATGGAGCGCGCTG
TCTGGCAAGCCCGGCCCTTTTCGAGCGCTGTGCCGCCGCACTGGCTGAATTTTCCGCCATCGTCGCCGCTGTGCCGGTCA
AGGACACCATCAAACAGGTATACCCCCAGAGCCGTCAGGTGGAGAAAACCATTCCCCGCGAGCAGCTCTGGGCGGCCCAG
ACGCCCCAGGGCGGCAATGTCGGGAGGCTGAAAGCCGCCCACGCCTGGGCCGCTGCCCAAGCGGTCGCCGTCACCGACGA
CGCGGCGCTATGCGAACTGCGCGGCGAGCCGGTGCAGGTCGTCCCCGGCGAGGAAACCAATCTCAAGATCACCACGCCTG
CAGATCTGTTGGTGGCCGAGGCTATCTTGAAAACACAGCTGCGCCAGGATCCCAGCCCATGA

Upstream 100 bases:

>100_bases
GTTTTCGGGGATTTACCGCCCCGGATCGCTTCGTCATCGGTTACGGCATGGATCTGGCCGAGCGTTACCGGGAGTTGCCT
TACGTGGGCGAACTGGAAGA

Downstream 100 bases:

>100_bases
GCGAACTGCCCCGCACCCTCGATGAGACCCTTGCGCAGTGCTCCCAGGCACTCGCAGCCACCCTCGAAAGCGGTAAAACC
CGCGCCCAAATTGACATCAA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MHLLIPAAGRGSRMGADGNKLLLPLSGRPLLAWTLAAAAQAPSTRWIGIIGQPMDEEAIRLLAQKLGLRVPVHWIEGGET
RQQSVYRGLLALPVEAQQVLIHDGARCLASPALFERCAAALAEFSAIVAAVPVKDTIKQVYPQSRQVEKTIPREQLWAAQ
TPQGGNVGRLKAAHAWAAAQAVAVTDDAALCELRGEPVQVVPGEETNLKITTPADLLVAEAILKTQLRQDPSP

Sequences:

>Translated_233_residues
MHLLIPAAGRGSRMGADGNKLLLPLSGRPLLAWTLAAAAQAPSTRWIGIIGQPMDEEAIRLLAQKLGLRVPVHWIEGGET
RQQSVYRGLLALPVEAQQVLIHDGARCLASPALFERCAAALAEFSAIVAAVPVKDTIKQVYPQSRQVEKTIPREQLWAAQ
TPQGGNVGRLKAAHAWAAAQAVAVTDDAALCELRGEPVQVVPGEETNLKITTPADLLVAEAILKTQLRQDPSP
>Mature_233_residues
MHLLIPAAGRGSRMGADGNKLLLPLSGRPLLAWTLAAAAQAPSTRWIGIIGQPMDEEAIRLLAQKLGLRVPVHWIEGGET
RQQSVYRGLLALPVEAQQVLIHDGARCLASPALFERCAAALAEFSAIVAAVPVKDTIKQVYPQSRQVEKTIPREQLWAAQ
TPQGGNVGRLKAAHAWAAAQAVAVTDDAALCELRGEPVQVVPGEETNLKITTPADLLVAEAILKTQLRQDPSP

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Escherichia coli, GI1789104, Length=231, Percent_Identity=32.034632034632, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_GLOVI (Q7NGU6)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925737.1
- ProteinModelPortal:   Q7NGU6
- GeneID:   2601192
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:glr2791
- NMPDR:   fig|251221.1.peg.2791
- HOGENOM:   HBG672839
- OMA:   SKVIVVC
- ProtClustDB:   PRK00155
- BioCyc:   GVIO251221:GLR2791-MONOMER
- BRENDA:   2.7.7.60
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 24894; Mature: 24894

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLLIPAAGRGSRMGADGNKLLLPLSGRPLLAWTLAAAAQAPSTRWIGIIGQPMDEEAIR
CEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHH
LLAQKLGLRVPVHWIEGGETRQQSVYRGLLALPVEAQQVLIHDGARCLASPALFERCAAA
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHCCHHHHHHHHHH
LAEFSAIVAAVPVKDTIKQVYPQSRQVEKTIPREQLWAAQTPQGGNVGRLKAAHAWAAAQ
HHHHHHHHHHCCHHHHHHHHCCCCCHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHE
AVAVTDDAALCELRGEPVQVVPGEETNLKITTPADLLVAEAILKTQLRQDPSP
EEEECCCHHHHHCCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHLLIPAAGRGSRMGADGNKLLLPLSGRPLLAWTLAAAAQAPSTRWIGIIGQPMDEEAIR
CEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHH
LLAQKLGLRVPVHWIEGGETRQQSVYRGLLALPVEAQQVLIHDGARCLASPALFERCAAA
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHCCHHHHHHHHHH
LAEFSAIVAAVPVKDTIKQVYPQSRQVEKTIPREQLWAAQTPQGGNVGRLKAAHAWAAAQ
HHHHHHHHHHCCHHHHHHHHCCCCCHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHE
AVAVTDDAALCELRGEPVQVVPGEETNLKITTPADLLVAEAILKTQLRQDPSP
EEEECCCHHHHHCCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292