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The map label for this gene is trpA

Identifier: 37522181

GI number: 37522181

Start: 2766779

End: 2767609

Strand: Reverse

Name: trpA

Synonym: gvip361

Alternate gene names: 37522181

Gene position: 2767609-2766779 (Counterclockwise)

Preceding gene: 37522185

Following gene: 37522179

Centisome position: 59.4

GC content: 67.39

Gene sequence:

>831_bases
ATGTCCAGTCTCGCTTCCCCCGGCCGCATCGCCCGCGTTTTTGCCGCCTTGCACGCGCGGCGGGAAGTCGCCTTTATCCC
GTTTATCACCGCGGGAGACCCGGATCTGGAGACCACCGCCGAGGCGCTGCTCACCCTCGACCGCAACGGTGCCGATCTGC
TGGAATTGGGCCTGCCTTACTCCGATCCGCTCGCCGACGGACCGACCATCCAGGCGGCGGCCACCCGCGCCCTCGCCCGG
GGCACCACCCCCGGCGCCGTGTTGGATCTGGTGGCCCGCCTCACCCCCGAGTTGCGCGCACCGCTGATTGTCTTCACCTA
CTTCAACTTGATCCTGGCGGTGGGAATCGAAGCATTTGTCGAGCGGCTCGCCGCCAGTGGCGCCAGCGGCCTGCTGGTGC
CGGATCTGCCCGTCGAAGAAGGCGATGCGCTCCAGACGGCCGCGAACGTCCAGGGGCTCGACGTCATCTGGCTGGTGGCA
CCGACCAGCCCCCCCGAGCGGCTCAGACGGATCGCCGAGCGCACCACCGGCTTTGTCTATCTGGTGAGCACCACCGGGGT
GACCGGCGCGCGCACCCAGGTCGCCTCCTCGGTGCGCACGTCCCTCGCCCAGTTGCGCGCCCTCACCACCCGTCCGGTGG
CCGTGGGTTTTGGCATCTCCACTCCTGAGCAAGCCCACGAAGTGGCTTCCCTGGGCGCCGACGGCGTCATTGTCGGTTCC
GCCTGCGTGCAACTGCTGGCCACCGCTGCCCCGGAAGAGCGCCTCGGGCAGCTCGCTGAGTTCTGTCGCCAATTGAAAAA
GGCCAGTCAAACTCTTCCTGTAAAAAGTTAA

Upstream 100 bases:

>100_bases
TGTCACCGGACGGTAACGGCATCGCCGCTTCATATTTCACAATCAACGTTAACACCGATCCCGCCGCCGGGAACCCCCGC
GCCACCCAAGGAGAACGCCC

Downstream 100 bases:

>100_bases
GGAACATTAAGCGGGCTCACGGCGAAAAGCCCCGCCGTGTATTCTCGGGGCAGAGGGAAACACGACAGAGCTCAGTCGGG
ATATGTCCGATTGTCCAGGG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSSLASPGRIARVFAALHARREVAFIPFITAGDPDLETTAEALLTLDRNGADLLELGLPYSDPLADGPTIQAAATRALAR
GTTPGAVLDLVARLTPELRAPLIVFTYFNLILAVGIEAFVERLAASGASGLLVPDLPVEEGDALQTAANVQGLDVIWLVA
PTSPPERLRRIAERTTGFVYLVSTTGVTGARTQVASSVRTSLAQLRALTTRPVAVGFGISTPEQAHEVASLGADGVIVGS
ACVQLLATAAPEERLGQLAEFCRQLKKASQTLPVKS

Sequences:

>Translated_276_residues
MSSLASPGRIARVFAALHARREVAFIPFITAGDPDLETTAEALLTLDRNGADLLELGLPYSDPLADGPTIQAAATRALAR
GTTPGAVLDLVARLTPELRAPLIVFTYFNLILAVGIEAFVERLAASGASGLLVPDLPVEEGDALQTAANVQGLDVIWLVA
PTSPPERLRRIAERTTGFVYLVSTTGVTGARTQVASSVRTSLAQLRALTTRPVAVGFGISTPEQAHEVASLGADGVIVGS
ACVQLLATAAPEERLGQLAEFCRQLKKASQTLPVKS
>Mature_275_residues
SSLASPGRIARVFAALHARREVAFIPFITAGDPDLETTAEALLTLDRNGADLLELGLPYSDPLADGPTIQAAATRALARG
TTPGAVLDLVARLTPELRAPLIVFTYFNLILAVGIEAFVERLAASGASGLLVPDLPVEEGDALQTAANVQGLDVIWLVAP
TSPPERLRRIAERTTGFVYLVSTTGVTGARTQVASSVRTSLAQLRALTTRPVAVGFGISTPEQAHEVASLGADGVIVGSA
CVQLLATAAPEERLGQLAEFCRQLKKASQTLPVKS

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=266, Percent_Identity=38.3458646616541, Blast_Score=169, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321412, Length=257, Percent_Identity=38.1322957198444, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_GLOVI (Q7NHC5)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925558.1
- ProteinModelPortal:   Q7NHC5
- SMR:   Q7NHC5
- GeneID:   2601013
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.2612
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- BioCyc:   GVIO251221:GLL2612-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28840; Mature: 28709

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA; PS00133 CARBOXYPEPT_ZN_2

Important sites: ACT_SITE 55-55 ACT_SITE 66-66

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLASPGRIARVFAALHARREVAFIPFITAGDPDLETTAEALLTLDRNGADLLELGLPY
CCCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
SDPLADGPTIQAAATRALARGTTPGAVLDLVARLTPELRAPLIVFTYFNLILAVGIEAFV
CCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHH
ERLAASGASGLLVPDLPVEEGDALQTAANVQGLDVIWLVAPTSPPERLRRIAERTTGFVY
HHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEE
LVSTTGVTGARTQVASSVRTSLAQLRALTTRPVAVGFGISTPEQAHEVASLGADGVIVGS
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCEEHHH
ACVQLLATAAPEERLGQLAEFCRQLKKASQTLPVKS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SSLASPGRIARVFAALHARREVAFIPFITAGDPDLETTAEALLTLDRNGADLLELGLPY
CCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
SDPLADGPTIQAAATRALARGTTPGAVLDLVARLTPELRAPLIVFTYFNLILAVGIEAFV
CCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHH
ERLAASGASGLLVPDLPVEEGDALQTAANVQGLDVIWLVAPTSPPERLRRIAERTTGFVY
HHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEE
LVSTTGVTGARTQVASSVRTSLAQLRALTTRPVAVGFGISTPEQAHEVASLGADGVIVGS
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCEEHHH
ACVQLLATAAPEERLGQLAEFCRQLKKASQTLPVKS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292