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The map label for this gene is nadE [H]

Identifier: 37522156

GI number: 37522156

Start: 2740050

End: 2741672

Strand: Direct

Name: nadE [H]

Synonym: glr2587

Alternate gene names: 37522156

Gene position: 2740050-2741672 (Clockwise)

Preceding gene: 37522153

Following gene: 37522158

Centisome position: 58.81

GC content: 65.31

Gene sequence:

>1623_bases
ATGCGAGTGGCGCTTTTGCAACTGAATTCGACCGTGGGGGATCTGGTCGGCAACGCCGGGCGCATCGAGCGGGCGGCGAG
GGAAGCTGCCGCCGCCGGGGCGGATCTGGCGATCACCCACGAGTTGGCCCTGCCCGGTTATCCGCCCCGGGATCTGCTGT
TGGATCGGGCCTTTGTGGCCGATGTCCAGCAGACCGCGCAGCGGCTTGCCCATGCGCTTGCCGGGGTGGTGCCGGTGTTG
GTGGGTACGGCGGTGCCGAGCGCGGTGGGGCGGCCCCTGGCCAACGCGGCTTTGCTGCTCGAAGGGGGAGAGTGCCGGGA
AACGGTGCTCAAGGGTCTGCTTCCTACCTACGACATCTTTGACGAGGACCGCTACTTCGAGGCGGGGCAGCGTGCCTATC
CGATCGAGATTGCCGGGGTGGCGATGGGGGTGCACGTCTGCGAGGACATCTGGAACGATCGCGAATTTTGGCCAAGGCCG
CGCTACCGCCGCGACCCGGTGGAGGAACTGGCCGGGCAGGGGGCGCGCTACTTGCTCAATCTTTCCAGTTCGCCTTTTTA
TGCGGGCAAGCAGCAGTTGCGCGAACGGCTTCTGGCCCATGCGGCCCGCCGCCACCGCCTGCCGGTACTCTACGTCAATC
AGGTGGGGGGCAACGACGAATTGCTCTTCGACGGCCGCAGTTGCGTTTTCGACGGGGAGGGCCGCCTGACCGCGCGGGCG
CGGGCGTTTGCCGAGGATATGCTGCTGGTGGATTTGGATTCGCTCGCCGGGCGCATCGAGCCTCAACCCACAGGCGAAGC
GGAAATCTGGGAAGCGTTAGTGATGGGTACAGCCGATTACGCCCGCAAGTGCGGCTTTCAGCAGGGGTTGGTGGCCCTTT
CCGGGGGCATCGATTCGGCCCTCACCCTGGCGATTGTGGCCGCCGCCCTGGGGCCTGTGAACGTGCTCGCGGTGATGATG
CCTTCGCCGTATTCCAGTGCCGGCAGCATCGACGACAGTCTGGCCCTCGCCGCCAATCTGGGGGTGGAGACGCTCAAATT
GCCCATCGCTCCGGCAATGGCGGCTTTTGATCAGATTCTGGCTCCGGCCTTTGCGGATCTGGCCGCCGATGTCACCGAGG
AGAATTTGCAGGCGCGCATCCGCGGCACCTTGATGATGGCGCTGTCCAATAAGTGGAACCGCCTGGTATTTATCACCGGC
AACAAGTCCGAGACCGCCGTCGGTTTTAACACCCTCTACGGTTGCACCGCCGGGGCACTGGCCGTGATTGCCGACTTGTA
CAAAGGCGAAGTCTACCGCCTGGCGCACTGGCTCAACCGGGACGGCGCGGTGATTCCCGAGGGTATCCTCACCAAGGCTC
CTTCCGCCGAGTTGCGCCCGGACCAGCGCGACTCCGACATCCTGCCGCCCTACGACGTGCTCGACGGCATTCTACGCGCC
CACCTCGAAGGTGGGCGCACCCCCGAGGAGATCGCCGGCGAAGGCTATCACCCAGAAGTGATCAAAAAAGTACTCGCCAT
GGTGCGCCGGGCCGAGTTCAAACGCAAGCAACTGCCGCCGGGTCTGCGCGTCTCGCCGCGCGCCTTCGGCATCGGCTGGC
GAATGCCCATCGCCCACGCCTGA

Upstream 100 bases:

>100_bases
GATGGCATTCCTTGTCGCGGATCAGGGGAAGGTCGGGCTGCAAGCCGCTGTTACGCTTGGAAGAACATTGATCAATCGGG
GACCAGCCGAGGGCGAACCC

Downstream 100 bases:

>100_bases
CTCCTAATTGGACGGCCAGAGGCGGGCGACGGGAACCTCCCAGCCCGCGAGCAACTCCGGCAGCCGCAACACATCGTCCT
CTTTGAGGATCGCCGCTTTG

Product: NH(3)-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MRVALLQLNSTVGDLVGNAGRIERAAREAAAAGADLAITHELALPGYPPRDLLLDRAFVADVQQTAQRLAHALAGVVPVL
VGTAVPSAVGRPLANAALLLEGGECRETVLKGLLPTYDIFDEDRYFEAGQRAYPIEIAGVAMGVHVCEDIWNDREFWPRP
RYRRDPVEELAGQGARYLLNLSSSPFYAGKQQLRERLLAHAARRHRLPVLYVNQVGGNDELLFDGRSCVFDGEGRLTARA
RAFAEDMLLVDLDSLAGRIEPQPTGEAEIWEALVMGTADYARKCGFQQGLVALSGGIDSALTLAIVAAALGPVNVLAVMM
PSPYSSAGSIDDSLALAANLGVETLKLPIAPAMAAFDQILAPAFADLAADVTEENLQARIRGTLMMALSNKWNRLVFITG
NKSETAVGFNTLYGCTAGALAVIADLYKGEVYRLAHWLNRDGAVIPEGILTKAPSAELRPDQRDSDILPPYDVLDGILRA
HLEGGRTPEEIAGEGYHPEVIKKVLAMVRRAEFKRKQLPPGLRVSPRAFGIGWRMPIAHA

Sequences:

>Translated_540_residues
MRVALLQLNSTVGDLVGNAGRIERAAREAAAAGADLAITHELALPGYPPRDLLLDRAFVADVQQTAQRLAHALAGVVPVL
VGTAVPSAVGRPLANAALLLEGGECRETVLKGLLPTYDIFDEDRYFEAGQRAYPIEIAGVAMGVHVCEDIWNDREFWPRP
RYRRDPVEELAGQGARYLLNLSSSPFYAGKQQLRERLLAHAARRHRLPVLYVNQVGGNDELLFDGRSCVFDGEGRLTARA
RAFAEDMLLVDLDSLAGRIEPQPTGEAEIWEALVMGTADYARKCGFQQGLVALSGGIDSALTLAIVAAALGPVNVLAVMM
PSPYSSAGSIDDSLALAANLGVETLKLPIAPAMAAFDQILAPAFADLAADVTEENLQARIRGTLMMALSNKWNRLVFITG
NKSETAVGFNTLYGCTAGALAVIADLYKGEVYRLAHWLNRDGAVIPEGILTKAPSAELRPDQRDSDILPPYDVLDGILRA
HLEGGRTPEEIAGEGYHPEVIKKVLAMVRRAEFKRKQLPPGLRVSPRAFGIGWRMPIAHA
>Mature_540_residues
MRVALLQLNSTVGDLVGNAGRIERAAREAAAAGADLAITHELALPGYPPRDLLLDRAFVADVQQTAQRLAHALAGVVPVL
VGTAVPSAVGRPLANAALLLEGGECRETVLKGLLPTYDIFDEDRYFEAGQRAYPIEIAGVAMGVHVCEDIWNDREFWPRP
RYRRDPVEELAGQGARYLLNLSSSPFYAGKQQLRERLLAHAARRHRLPVLYVNQVGGNDELLFDGRSCVFDGEGRLTARA
RAFAEDMLLVDLDSLAGRIEPQPTGEAEIWEALVMGTADYARKCGFQQGLVALSGGIDSALTLAIVAAALGPVNVLAVMM
PSPYSSAGSIDDSLALAANLGVETLKLPIAPAMAAFDQILAPAFADLAADVTEENLQARIRGTLMMALSNKWNRLVFITG
NKSETAVGFNTLYGCTAGALAVIADLYKGEVYRLAHWLNRDGAVIPEGILTKAPSAELRPDQRDSDILPPYDVLDGILRA
HLEGGRTPEEIAGEGYHPEVIKKVLAMVRRAEFKRKQLPPGLRVSPRAFGIGWRMPIAHA

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 58272; Mature: 58272

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVALLQLNSTVGDLVGNAGRIERAAREAAAAGADLAITHELALPGYPPRDLLLDRAFVA
CEEEEEECCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHH
DVQQTAQRLAHALAGVVPVLVGTAVPSAVGRPLANAALLLEGGECRETVLKGLLPTYDIF
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCCCC
DEDRYFEAGQRAYPIEIAGVAMGVHVCEDIWNDREFWPRPRYRRDPVEELAGQGARYLLN
CCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCEEEEE
LSSSPFYAGKQQLRERLLAHAARRHRLPVLYVNQVGGNDELLFDGRSCVFDGEGRLTARA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCEEEECCCCCCHHHH
RAFAEDMLLVDLDSLAGRIEPQPTGEAEIWEALVMGTADYARKCGFQQGLVALSGGIDSA
HHHHHHHHEEEHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCHHHCCEEECCCCHHH
LTLAIVAAALGPVNVLAVMMPSPYSSAGSIDDSLALAANLGVETLKLPIAPAMAAFDQIL
HHHHHHHHHHCCHHEEEEECCCCCCCCCCCCHHHHHHHHCCCHHEECCCHHHHHHHHHHH
APAFADLAADVTEENLQARIRGTLMMALSNKWNRLVFITGNKSETAVGFNTLYGCTAGAL
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCEECHHHHHHHHHHHH
AVIADLYKGEVYRLAHWLNRDGAVIPEGILTKAPSAELRPDQRDSDILPPYDVLDGILRA
HHHHHHHHCHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
HLEGGRTPEEIAGEGYHPEVIKKVLAMVRRAEFKRKQLPPGLRVSPRAFGIGWRMPIAHA
HHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEECCEECCCCCCC
>Mature Secondary Structure
MRVALLQLNSTVGDLVGNAGRIERAAREAAAAGADLAITHELALPGYPPRDLLLDRAFVA
CEEEEEECCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHH
DVQQTAQRLAHALAGVVPVLVGTAVPSAVGRPLANAALLLEGGECRETVLKGLLPTYDIF
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCCCC
DEDRYFEAGQRAYPIEIAGVAMGVHVCEDIWNDREFWPRPRYRRDPVEELAGQGARYLLN
CCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCEEEEE
LSSSPFYAGKQQLRERLLAHAARRHRLPVLYVNQVGGNDELLFDGRSCVFDGEGRLTARA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCEEEECCCCCCHHHH
RAFAEDMLLVDLDSLAGRIEPQPTGEAEIWEALVMGTADYARKCGFQQGLVALSGGIDSA
HHHHHHHHEEEHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCHHHCCEEECCCCHHH
LTLAIVAAALGPVNVLAVMMPSPYSSAGSIDDSLALAANLGVETLKLPIAPAMAAFDQIL
HHHHHHHHHHCCHHEEEEECCCCCCCCCCCCHHHHHHHHCCCHHEECCCHHHHHHHHHHH
APAFADLAADVTEENLQARIRGTLMMALSNKWNRLVFITGNKSETAVGFNTLYGCTAGAL
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCEECHHHHHHHHHHHH
AVIADLYKGEVYRLAHWLNRDGAVIPEGILTKAPSAELRPDQRDSDILPPYDVLDGILRA
HHHHHHHHCHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
HLEGGRTPEEIAGEGYHPEVIKKVLAMVRRAEFKRKQLPPGLRVSPRAFGIGWRMPIAHA
HHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEECCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]