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The map label for this gene is pdhC [H]

Identifier: 37522138

GI number: 37522138

Start: 2719813

End: 2721072

Strand: Reverse

Name: pdhC [H]

Synonym: gll2569

Alternate gene names: 37522138

Gene position: 2721072-2719813 (Counterclockwise)

Preceding gene: 37522139

Following gene: 37522137

Centisome position: 58.4

GC content: 66.11

Gene sequence:

>1260_bases
ATGATACGCGAAGTCACGATGCCCGCCCTGTCCTCGACGATGACCGAGGGCAAGATCGTCACCTGGAAGAAGCAGGAGGG
AGATGCGGTCAGCCGCAGCGACATCCTGCTGGTCGTCGAGTCCGACAAGGCGGACATGGATGTCGAGTCTTTTGACGAGG
GCATCCTCGCGAACATCCTGGTAAGCGACGGCGGCAGCGCCCCGGTAGGCTCGGTGATCGCCCTGATTGCCGAGACCGAG
GCGGAGGTGGCAGAGGCAAAAAAACGGCCCCCAAGCGGCACTGCCGCCGCACCACCTGCGACGGTTCCCACCCCGGCACC
GGCGCCGAGTGCTCCTGCCCCGGTGGCGGCCGCCACAACTCCAGTCTCCTCCGGCAGCAACGGCGGCCGGATCGTCGCTT
CTCCCAACGCCCGCCGCCTCGCTGAACAACTGGGGGTGGATCTGGCGAGCATCACGGGCAGCGGTCCCGGTGGCCGGATC
GTGGGTGAAGATGTCGAGCGCGCTGCGGCAGGCGCCAAGGCTCCGGCCCCCGCCCCCGCCGCCAAACCGGCGTCCGCCCC
TGCCCCGCTCCCGGCTGCGGCGGCGAGCGGTCAGCCGGTGGCCTTCAGCGCCCTGCAGCAGGCGGTCAACCGTAACATGG
AAGCGGCCCTGGCCATCCCGGCTTTTCGGGTCGGTTATACGATCACGACCGATGCTTTCGACGAACTGCACAAGTCGGTC
AAATCGAAGGGCGTGACGGTGACCACGATGCTGGTGAAGGCCGTGGCCATTACCCTCGCCAAGCACCCGCTGCTGTTTGC
CGCCTACACCGAATCCGGCCTCCGCTACCATTCGGCGGTCAATGTCGCAGTGGCGGTGGCGATGGAGGAGGGGGGGCTGA
TCACCCCGGTGCTGCGCGCCGCCGACAGCAAAGATCTCTATACCCTGGCGCGCGAGTGGAAAGATCTGGTGGAGCGCGCC
CGCCTCAAGAAGCTGCAGCCGGAGGAGTACACGAGCGGCAACTTCACCCTGTCGAATCTGGGCATGTTCGGGGTCGACCG
CTTCGATGCCATTGTGCCCCCCGGCACCAGCGCCATTCTGGCTATCGGTGCCGCCAAACCGACGGTGGTAGTTACCGAGG
CGGGCCACATCGCCATTCAAAAACAGATGCAAGTCAACTTGAGCGGCGACCACCGGGTCTTCTACGGCACCGACGGGGCG
CGCTTCCTGCAGGATCTGGCCAAGCTCATCGAGCAATCGCCGCAGCAGTTGACGCTTTGA

Upstream 100 bases:

>100_bases
CTTCTGGCTCACCCGAACCGCCGCCGCCGCACCGCCGGGGGTGGCGGTTTTTTAGAAGCGGAGATGATAGTATCTGTTGT
GATTTGTTACGCAGACGCCG

Downstream 100 bases:

>100_bases
TGATCGATTGTCCGGACGCCGTTTTGCGAGGAGAGAGTTGCCATGGCTTTGCAGATTAAGATTGTCGCTCCCAACAAAGT
CGTCTTCGACGACCAGGTCG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MIREVTMPALSSTMTEGKIVTWKKQEGDAVSRSDILLVVESDKADMDVESFDEGILANILVSDGGSAPVGSVIALIAETE
AEVAEAKKRPPSGTAAAPPATVPTPAPAPSAPAPVAAATTPVSSGSNGGRIVASPNARRLAEQLGVDLASITGSGPGGRI
VGEDVERAAAGAKAPAPAPAAKPASAPAPLPAAAASGQPVAFSALQQAVNRNMEAALAIPAFRVGYTITTDAFDELHKSV
KSKGVTVTTMLVKAVAITLAKHPLLFAAYTESGLRYHSAVNVAVAVAMEEGGLITPVLRAADSKDLYTLAREWKDLVERA
RLKKLQPEEYTSGNFTLSNLGMFGVDRFDAIVPPGTSAILAIGAAKPTVVVTEAGHIAIQKQMQVNLSGDHRVFYGTDGA
RFLQDLAKLIEQSPQQLTL

Sequences:

>Translated_419_residues
MIREVTMPALSSTMTEGKIVTWKKQEGDAVSRSDILLVVESDKADMDVESFDEGILANILVSDGGSAPVGSVIALIAETE
AEVAEAKKRPPSGTAAAPPATVPTPAPAPSAPAPVAAATTPVSSGSNGGRIVASPNARRLAEQLGVDLASITGSGPGGRI
VGEDVERAAAGAKAPAPAPAAKPASAPAPLPAAAASGQPVAFSALQQAVNRNMEAALAIPAFRVGYTITTDAFDELHKSV
KSKGVTVTTMLVKAVAITLAKHPLLFAAYTESGLRYHSAVNVAVAVAMEEGGLITPVLRAADSKDLYTLAREWKDLVERA
RLKKLQPEEYTSGNFTLSNLGMFGVDRFDAIVPPGTSAILAIGAAKPTVVVTEAGHIAIQKQMQVNLSGDHRVFYGTDGA
RFLQDLAKLIEQSPQQLTL
>Mature_419_residues
MIREVTMPALSSTMTEGKIVTWKKQEGDAVSRSDILLVVESDKADMDVESFDEGILANILVSDGGSAPVGSVIALIAETE
AEVAEAKKRPPSGTAAAPPATVPTPAPAPSAPAPVAAATTPVSSGSNGGRIVASPNARRLAEQLGVDLASITGSGPGGRI
VGEDVERAAAGAKAPAPAPAAKPASAPAPLPAAAASGQPVAFSALQQAVNRNMEAALAIPAFRVGYTITTDAFDELHKSV
KSKGVTVTTMLVKAVAITLAKHPLLFAAYTESGLRYHSAVNVAVAVAMEEGGLITPVLRAADSKDLYTLAREWKDLVERA
RLKKLQPEEYTSGNFTLSNLGMFGVDRFDAIVPPGTSAILAIGAAKPTVVVTEAGHIAIQKQMQVNLSGDHRVFYGTDGA
RFLQDLAKLIEQSPQQLTL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=434, Percent_Identity=34.5622119815668, Blast_Score=205, Evalue=8e-53,
Organism=Homo sapiens, GI203098816, Length=449, Percent_Identity=32.0712694877506, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI203098753, Length=449, Percent_Identity=32.0712694877506, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI110671329, Length=444, Percent_Identity=27.027027027027, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI260898739, Length=158, Percent_Identity=34.1772151898734, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI19923748, Length=173, Percent_Identity=31.2138728323699, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786946, Length=426, Percent_Identity=29.81220657277, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1786305, Length=436, Percent_Identity=28.6697247706422, Blast_Score=111, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17560088, Length=446, Percent_Identity=35.4260089686099, Blast_Score=210, Evalue=9e-55,
Organism=Caenorhabditis elegans, GI17537937, Length=411, Percent_Identity=27.7372262773723, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17538894, Length=293, Percent_Identity=30.3754266211604, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI25146366, Length=421, Percent_Identity=28.5035629453682, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=32.739420935412, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6320352, Length=433, Percent_Identity=24.9422632794457, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321632, Length=176, Percent_Identity=33.5227272727273, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI20129315, Length=440, Percent_Identity=34.3181818181818, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24582497, Length=430, Percent_Identity=33.7209302325581, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI18859875, Length=428, Percent_Identity=26.1682242990654, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24645909, Length=166, Percent_Identity=28.9156626506024, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 43265; Mature: 43265

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIREVTMPALSSTMTEGKIVTWKKQEGDAVSRSDILLVVESDKADMDVESFDEGILANIL
CCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHCCCCEEEEE
VSDGGSAPVGSVIALIAETEAEVAEAKKRPPSGTAAAPPATVPTPAPAPSAPAPVAAATT
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECC
PVSSGSNGGRIVASPNARRLAEQLGVDLASITGSGPGGRIVGEDVERAAAGAKAPAPAPA
CCCCCCCCCEEEECCCHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCC
AKPASAPAPLPAAAASGQPVAFSALQQAVNRNMEAALAIPAFRVGYTITTDAFDELHKSV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCEEEECEEEEHHHHHHHHHHH
KSKGVTVTTMLVKAVAITLAKHPLLFAAYTESGLRYHSAVNVAVAVAMEEGGLITPVLRA
HHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEEHHEEEEEEEEEECCCCEEHHHHHC
ADSKDLYTLAREWKDLVERARLKKLQPEEYTSGNFTLSNLGMFGVDRFDAIVPPGTSAIL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCHHCCCCCCCCCEEE
AIGAAKPTVVVTEAGHIAIQKQMQVNLSGDHRVFYGTDGARFLQDLAKLIEQSPQQLTL
EEECCCCEEEEECCCCEEEEEEEEEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIREVTMPALSSTMTEGKIVTWKKQEGDAVSRSDILLVVESDKADMDVESFDEGILANIL
CCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHCCCCEEEEE
VSDGGSAPVGSVIALIAETEAEVAEAKKRPPSGTAAAPPATVPTPAPAPSAPAPVAAATT
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECC
PVSSGSNGGRIVASPNARRLAEQLGVDLASITGSGPGGRIVGEDVERAAAGAKAPAPAPA
CCCCCCCCCEEEECCCHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCC
AKPASAPAPLPAAAASGQPVAFSALQQAVNRNMEAALAIPAFRVGYTITTDAFDELHKSV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCEEEECEEEEHHHHHHHHHHH
KSKGVTVTTMLVKAVAITLAKHPLLFAAYTESGLRYHSAVNVAVAVAMEEGGLITPVLRA
HHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEEHHEEEEEEEEEECCCCEEHHHHHC
ADSKDLYTLAREWKDLVERARLKKLQPEEYTSGNFTLSNLGMFGVDRFDAIVPPGTSAIL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCHHCCCCCCCCCEEE
AIGAAKPTVVVTEAGHIAIQKQMQVNLSGDHRVFYGTDGARFLQDLAKLIEQSPQQLTL
EEECCCCEEEEECCCCEEEEEEEEEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA