Click here to switch to the map view.

The map label for this gene is yafC [C]

Identifier: 37522120

GI number: 37522120

Start: 2706360

End: 2707310

Strand: Direct

Name: yafC [C]

Synonym: glr2551

Alternate gene names: 37522120

Gene position: 2706360-2707310 (Clockwise)

Preceding gene: 37522117

Following gene: 37522122

Centisome position: 58.09

GC content: 63.72

Gene sequence:

>951_bases
ATGGATCGCATCGCCTGCATGCGCAGTTTCGTCCGCGCCGTCGAGACCGGCAGCTTCTCGGCGGTGGCGCGGGAAATGGC
TACCACCCAGCCGACCGTCAGCAAGCAAATCGCGGCATTGGAGGAGCACCTGGGTGCGCGGCTATTGGTGCGCTCGACCC
GCAGGCTCGGCCCCACCGAGGAGGGTGAGCGCTACTACCGCCAGTGCAGACGGGTGCTGGAGGCTATCGAGGAAGCGGAA
GCGAGCGTCCGCAGCGCCGAGCGACCGTTCGGGCGCCTGCGGGTGAGTTGCCCGGTCGCTTTTGGCCAATACCGGATCGT
GCCCAGGGTGAAAGAGTTTCTCGATCGCTACCCAGATATTGAACTGGATCTGACGATGACCGACGGGTTCGTCGATTTGA
TCGAAGCAGGGGCGGAGTTGGCCATCCGCATTGGGCTACTCGAAGACAGCTCCCTGGTGGCCCACCGCATCGGCACGACG
CGCCGGGTCACCGTCGGATCGGCGGCCTATTTTGCCGGGGCCGGGGAACCTCGCACCCTCGAGGATCTGACCCGGCACAA
CTGCCTGGTTTACACACACCTGGCGACGGGCAACACCTGGTCATTTGAGAGCCAGAGCGGACCGGTGCAGGTGAAAGTGC
GCGGCAATTTTCAATGCAACAATTCCGCAGCGATCCGCGAGGCGGTGCTGGCGGGTCTCGGTGTCGCCGTTTCGCCTGTC
TGGCTGTTTGGGGAGGATCTGGACCGGGGGGTACTCCAGGTCGTGTTGCACGATTTTCAACCGCCGCCGCTGCCCATCCA
CGCGGTCTATCTCAAAGACCGCTACCAACCGGCCCGGGTCCGCTGTTTCGTCGATTTTCTGGCGGCTGCTTTTAGAGGCG
ATCCCTGGGTCTGCAGCCAGGGGCCGTGGCCGGAAAAATCGCCGGCTTTTGTAAGAGAGCTGGTGGGCTAG

Upstream 100 bases:

>100_bases
GCAAAATCCTCGCCTGACAAAACTCTAAATTCATTCATATAGCTTTGAGAAGATGGAAAAAAGCCATAACATATATTCCA
AGTAAGTATGAATTCAGGTC

Downstream 100 bases:

>100_bases
GGGCGGGTCAGAATAAAAATCGTGCCGCGATTGCCGAGGGCGATGCGCAAGTCGGTGTCGATGTAGAGCGTATCGACGCT
TCCTTCGCTGCGAACATCGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MDRIACMRSFVRAVETGSFSAVAREMATTQPTVSKQIAALEEHLGARLLVRSTRRLGPTEEGERYYRQCRRVLEAIEEAE
ASVRSAERPFGRLRVSCPVAFGQYRIVPRVKEFLDRYPDIELDLTMTDGFVDLIEAGAELAIRIGLLEDSSLVAHRIGTT
RRVTVGSAAYFAGAGEPRTLEDLTRHNCLVYTHLATGNTWSFESQSGPVQVKVRGNFQCNNSAAIREAVLAGLGVAVSPV
WLFGEDLDRGVLQVVLHDFQPPPLPIHAVYLKDRYQPARVRCFVDFLAAAFRGDPWVCSQGPWPEKSPAFVRELVG

Sequences:

>Translated_316_residues
MDRIACMRSFVRAVETGSFSAVAREMATTQPTVSKQIAALEEHLGARLLVRSTRRLGPTEEGERYYRQCRRVLEAIEEAE
ASVRSAERPFGRLRVSCPVAFGQYRIVPRVKEFLDRYPDIELDLTMTDGFVDLIEAGAELAIRIGLLEDSSLVAHRIGTT
RRVTVGSAAYFAGAGEPRTLEDLTRHNCLVYTHLATGNTWSFESQSGPVQVKVRGNFQCNNSAAIREAVLAGLGVAVSPV
WLFGEDLDRGVLQVVLHDFQPPPLPIHAVYLKDRYQPARVRCFVDFLAAAFRGDPWVCSQGPWPEKSPAFVRELVG
>Mature_316_residues
MDRIACMRSFVRAVETGSFSAVAREMATTQPTVSKQIAALEEHLGARLLVRSTRRLGPTEEGERYYRQCRRVLEAIEEAE
ASVRSAERPFGRLRVSCPVAFGQYRIVPRVKEFLDRYPDIELDLTMTDGFVDLIEAGAELAIRIGLLEDSSLVAHRIGTT
RRVTVGSAAYFAGAGEPRTLEDLTRHNCLVYTHLATGNTWSFESQSGPVQVKVRGNFQCNNSAAIREAVLAGLGVAVSPV
WLFGEDLDRGVLQVVLHDFQPPPLPIHAVYLKDRYQPARVRCFVDFLAAAFRGDPWVCSQGPWPEKSPAFVRELVG

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=34.0277777777778, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=30.0341296928328, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=34.7972972972973, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI87081978, Length=287, Percent_Identity=32.404181184669, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=29.8245614035088, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=26.8551236749117, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=28.6206896551724, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1789173, Length=258, Percent_Identity=28.6821705426357, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788706, Length=239, Percent_Identity=25.5230125523013, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI157672245, Length=116, Percent_Identity=34.4827586206897, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790011, Length=305, Percent_Identity=25.5737704918033, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789204, Length=118, Percent_Identity=33.0508474576271, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35121; Mature: 35121

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRIACMRSFVRAVETGSFSAVAREMATTQPTVSKQIAALEEHLGARLLVRSTRRLGPTE
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCC
EGERYYRQCRRVLEAIEEAEASVRSAERPFGRLRVSCPVAFGQYRIVPRVKEFLDRYPDI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEECCCEEECHHHHHHHHHCCCC
ELDLTMTDGFVDLIEAGAELAIRIGLLEDSSLVAHRIGTTRRVTVGSAAYFAGAGEPRTL
EEEEEECCHHHHHHHCCCEEEEEEEEECCCHHHHHHCCCCEEEEECCHHEECCCCCCCHH
EDLTRHNCLVYTHLATGNTWSFESQSGPVQVKVRGNFQCNNSAAIREAVLAGLGVAVSPV
HHHHHCCEEEEEEECCCCEECCCCCCCCEEEEEECCEEECCHHHHHHHHHHHCCHHHCCC
WLFGEDLDRGVLQVVLHDFQPPPLPIHAVYLKDRYQPARVRCFVDFLAAAFRGDPWVCSQ
EEECCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEECC
GPWPEKSPAFVRELVG
CCCCCCCHHHHHHHCC
>Mature Secondary Structure
MDRIACMRSFVRAVETGSFSAVAREMATTQPTVSKQIAALEEHLGARLLVRSTRRLGPTE
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCC
EGERYYRQCRRVLEAIEEAEASVRSAERPFGRLRVSCPVAFGQYRIVPRVKEFLDRYPDI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEECCCEEECHHHHHHHHHCCCC
ELDLTMTDGFVDLIEAGAELAIRIGLLEDSSLVAHRIGTTRRVTVGSAAYFAGAGEPRTL
EEEEEECCHHHHHHHCCCEEEEEEEEECCCHHHHHHCCCCEEEEECCHHEECCCCCCCHH
EDLTRHNCLVYTHLATGNTWSFESQSGPVQVKVRGNFQCNNSAAIREAVLAGLGVAVSPV
HHHHHCCEEEEEEECCCCEECCCCCCCCEEEEEECCEEECCHHHHHHHHHHHCCHHHCCC
WLFGEDLDRGVLQVVLHDFQPPPLPIHAVYLKDRYQPARVRCFVDFLAAAFRGDPWVCSQ
EEECCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEECC
GPWPEKSPAFVRELVG
CCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]