Click here to switch to the map view.

The map label for this gene is mdh

Identifier: 37522111

GI number: 37522111

Start: 2696327

End: 2697304

Strand: Reverse

Name: mdh

Synonym: gll2542

Alternate gene names: 37522111

Gene position: 2697304-2696327 (Counterclockwise)

Preceding gene: 37522113

Following gene: 37522110

Centisome position: 57.89

GC content: 63.19

Gene sequence:

>978_bases
ATGGCCGCTCGTTCCTGCCGGGAAAGCAAAGTGTCGATCCTGGGTGCGGGCAATGTCGGAAGCGCCCTTGCCCAAAGGCT
CATCCAGGGCAACGTTGCCGATGTAGTGTTGCTTGATATCGTCGAAGGCCGTCCCCAGGGGATCACCCTCGACCTGCTTG
AAGCCTGCGGCGTCGAGGGACACACTTGTCGGATCACCGGCACCAACGACTACGCCCAGACCGCCGGGTCGGATGTGCTG
GTGGTGGCGGCCGGATTTGCCCGCCAGCCCGGCATGAGCCGCGACGACCTGCTGCTGACCAACACCCGCATCGTTTTTGA
GGTGACGCAAAAGGCGGTTGCCCACTCCCCGGAGGCAACCGTGGTGGTGGTTACCAATCCCCTCGACGCGATGAGCCATG
TCGCCTGGCGGGCGAGTGGTCTTGTCCCTGAACGGGTAATGGGCATGGCGGGGGTGCTCGATGCCGCCCGCTTCGAAACA
TTTATTGCCTGGGAACTGGGATTTTCGGTGCGCGATATCCGGGCGATGGTGTTGGGCGGACATGGAGATCTGATGGTGCC
GCTGCCTCGTTACACCACCATCTCGGGCGTTCCTCTGACACAACTGTTGAGTTCCGCCCGGATCGACGCACTGATCGAGC
GCACCCGCACCGGCGGAGCTGAGATTGTTCATCTGCTCAAGCGCGGCGGCGCTTACTATGCCCCGGCCGCCGCCGCCGCC
CGGATGATCGAGACTTTGTTGGGCGACGAACGGCGTCTGCTGCCGGTCGCCGCCTACCTGAGCGGCGAGTACGGTTTGAG
GAACATCCACATGGGTGTGCCGGTCATCCTCAGCCGCCACGGTGTCGAGCGGGTGGTCGAACTGACTCTGGAGACGGAGG
AAAAAGCTGCACTGTACCGCTCCGCCCATACGATCCGCGCCAGCCTCGATCAGATTGCTCACCTGATGCCTGCAAACCAG
CCGCCATCGGCGGTGTAA

Upstream 100 bases:

>100_bases
TGGTGTAGATCTACCCCGGGTGCATCGGCAGTTCGGCAGCAGCGCCGACCCGGGCAAGTGTGCCGGCAAAGGAGTAAAAT
AGGCTCCAGGAGGGGATTCT

Downstream 100 bases:

>100_bases
GATCGGCTGGCGCGGGTGTCAAAATGCGGAGTTTTTTTTATTTCCAATTCGCCAGTACAGGAGTAACATTTTTGGATACA
AGGCTCCTCAAGAATGAAAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MAARSCRESKVSILGAGNVGSALAQRLIQGNVADVVLLDIVEGRPQGITLDLLEACGVEGHTCRITGTNDYAQTAGSDVL
VVAAGFARQPGMSRDDLLLTNTRIVFEVTQKAVAHSPEATVVVVTNPLDAMSHVAWRASGLVPERVMGMAGVLDAARFET
FIAWELGFSVRDIRAMVLGGHGDLMVPLPRYTTISGVPLTQLLSSARIDALIERTRTGGAEIVHLLKRGGAYYAPAAAAA
RMIETLLGDERRLLPVAAYLSGEYGLRNIHMGVPVILSRHGVERVVELTLETEEKAALYRSAHTIRASLDQIAHLMPANQ
PPSAV

Sequences:

>Translated_325_residues
MAARSCRESKVSILGAGNVGSALAQRLIQGNVADVVLLDIVEGRPQGITLDLLEACGVEGHTCRITGTNDYAQTAGSDVL
VVAAGFARQPGMSRDDLLLTNTRIVFEVTQKAVAHSPEATVVVVTNPLDAMSHVAWRASGLVPERVMGMAGVLDAARFET
FIAWELGFSVRDIRAMVLGGHGDLMVPLPRYTTISGVPLTQLLSSARIDALIERTRTGGAEIVHLLKRGGAYYAPAAAAA
RMIETLLGDERRLLPVAAYLSGEYGLRNIHMGVPVILSRHGVERVVELTLETEEKAALYRSAHTIRASLDQIAHLMPANQ
PPSAV
>Mature_324_residues
AARSCRESKVSILGAGNVGSALAQRLIQGNVADVVLLDIVEGRPQGITLDLLEACGVEGHTCRITGTNDYAQTAGSDVLV
VAAGFARQPGMSRDDLLLTNTRIVFEVTQKAVAHSPEATVVVVTNPLDAMSHVAWRASGLVPERVMGMAGVLDAARFETF
IAWELGFSVRDIRAMVLGGHGDLMVPLPRYTTISGVPLTQLLSSARIDALIERTRTGGAEIVHLLKRGGAYYAPAAAAAR
MIETLLGDERRLLPVAAYLSGEYGLRNIHMGVPVILSRHGVERVVELTLETEEKAALYRSAHTIRASLDQIAHLMPANQP
PSAV

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=306, Percent_Identity=33.0065359477124, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI221136809, Length=306, Percent_Identity=33.0065359477124, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI15082234, Length=311, Percent_Identity=30.8681672025724, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI5031857, Length=305, Percent_Identity=31.1475409836066, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI260099723, Length=305, Percent_Identity=31.1475409836066, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI291575128, Length=306, Percent_Identity=31.0457516339869, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI4557032, Length=306, Percent_Identity=31.0457516339869, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI9257228, Length=307, Percent_Identity=30.6188925081433, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI4504973, Length=307, Percent_Identity=30.6188925081433, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI260099725, Length=196, Percent_Identity=33.1632653061224, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI260099727, Length=217, Percent_Identity=31.3364055299539, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI207028494, Length=189, Percent_Identity=31.2169312169312, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI21735621, Length=219, Percent_Identity=30.1369863013699, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI103472011, Length=309, Percent_Identity=23.9482200647249, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1789632, Length=317, Percent_Identity=28.7066246056782, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17535107, Length=291, Percent_Identity=28.1786941580756, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17554310, Length=293, Percent_Identity=30.3754266211604, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322765, Length=292, Percent_Identity=27.7397260273973, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320125, Length=261, Percent_Identity=28.3524904214559, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17136226, Length=303, Percent_Identity=30.03300330033, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI45550422, Length=313, Percent_Identity=27.1565495207668, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24647881, Length=324, Percent_Identity=26.8518518518519, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24663599, Length=317, Percent_Identity=26.4984227129338, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24663595, Length=297, Percent_Identity=26.2626262626263, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_GLOVI (Q7NHJ3)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925488.1
- ProteinModelPortal:   Q7NHJ3
- SMR:   Q7NHJ3
- GeneID:   2600943
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll2542
- NMPDR:   fig|251221.1.peg.2542
- HOGENOM:   HBG566126
- OMA:   SINLKVM
- BioCyc:   GVIO251221:GLL2542-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 34686; Mature: 34554

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 39-39 BINDING 88-88 BINDING 94-94 BINDING 101-101 BINDING 126-126 BINDING 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARSCRESKVSILGAGNVGSALAQRLIQGNVADVVLLDIVEGRPQGITLDLLEACGVEG
CCCCCCCCCCEEEEECCCHHHHHHHHHHCCCHHHEEEEEECCCCCCCCCHHHHHHCCCCC
HTCRITGTNDYAQTAGSDVLVVAAGFARQPGMSRDDLLLTNTRIVFEVTQKAVAHSPEAT
CEEEEECCCHHHHHCCCCEEEEEECCCCCCCCCCCCEEEECCEEEEEEHHHHHHCCCCCE
VVVVTNPLDAMSHVAWRASGLVPERVMGMAGVLDAARFETFIAWELGFSVRDIRAMVLGG
EEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCHHEEEEEEECC
HGDLMVPLPRYTTISGVPLTQLLSSARIDALIERTRTGGAEIVHLLKRGGAYYAPAAAAA
CCCEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHH
RMIETLLGDERRLLPVAAYLSGEYGLRNIHMGVPVILSRHGVERVVELTLETEEKAALYR
HHHHHHHCCCCCEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
SAHTIRASLDQIAHLMPANQPPSAV
HHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AARSCRESKVSILGAGNVGSALAQRLIQGNVADVVLLDIVEGRPQGITLDLLEACGVEG
CCCCCCCCCEEEEECCCHHHHHHHHHHCCCHHHEEEEEECCCCCCCCCHHHHHHCCCCC
HTCRITGTNDYAQTAGSDVLVVAAGFARQPGMSRDDLLLTNTRIVFEVTQKAVAHSPEAT
CEEEEECCCHHHHHCCCCEEEEEECCCCCCCCCCCCEEEECCEEEEEEHHHHHHCCCCCE
VVVVTNPLDAMSHVAWRASGLVPERVMGMAGVLDAARFETFIAWELGFSVRDIRAMVLGG
EEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCHHEEEEEEECC
HGDLMVPLPRYTTISGVPLTQLLSSARIDALIERTRTGGAEIVHLLKRGGAYYAPAAAAA
CCCEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHH
RMIETLLGDERRLLPVAAYLSGEYGLRNIHMGVPVILSRHGVERVVELTLETEEKAALYR
HHHHHHHCCCCCEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
SAHTIRASLDQIAHLMPANQPPSAV
HHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292