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The map label for this gene is proS

Identifier: 37522094

GI number: 37522094

Start: 2680379

End: 2682148

Strand: Reverse

Name: proS

Synonym: gll2525

Alternate gene names: 37522094

Gene position: 2682148-2680379 (Counterclockwise)

Preceding gene: 37522097

Following gene: 37522093

Centisome position: 57.57

GC content: 64.86

Gene sequence:

>1770_bases
ATGACCCGCCTCAGTTCGAGCTTCTTTTTCACCCTGCGCGAGGCGCCCGCCGAAGCCGTCGCCGTCAGCCACAAGTTGTT
GTTGCGCGCGGGGTTCATCCGGCCCTTGGCGGGTACGGCGGGCCTGTACGCTTACGGGCCGCTCATGCAGCGCGTGCTCC
AAAAAGTCGGCCGCATCGTGCGCGAGGAGATGGATGCCACGGGTGCCCAGGAGGCGCTGTTCTGTCAGTTGCAGCCTGCA
GAAATCTGGAAGGAATCCGGGCGCTGGACCGTCTATACCCAGGACGGCACGATGTTTACCCTCAAGGACGGCCAGGGCGA
GCAGGCCCGCGAGTATGGCCTCGGTCCCACCCACGAAGAAGCTGTCTGCGACTTTGTGCGTGCGAGCCTCAATTCTTATA
AGCAGCTGCCCTTTCACCTCTATCAGGTGCAGACGAAGTTTCGCAACGAGAAGCGGCCCCGCTTCGGGTTGATGCGCGGG
CGCGAATTTATCATGAAGGACGGTTACTCCTTCCATGCCACGCCCGAGAGCCTCGATGAAACCTACCGGGCGATGTACCG
CGCCTATACGAATATGTTTCGCCGCTGCGGGCTCGATTTTCGGGCAGTGGAAGCCGACTCCGGGGCCATCGGCGGTTCCG
GGTCGCACGAATTTATGGCGCTGTGCGACATCGGCGAGGATACGATCCTCTACTGCGACGCGGCGGGCTACGCGGCCAAC
GTCGAGAAGGCCGTCTCCCTGGTGAGCGACCCAGAAGCGATCGGCCCCGGCAGCTACGCCGTCAAATCCACCCCCGGCAT
CCGCACCGTCGAGCAGCAGGCGGCGATGCTCGGGGTGCCGATAAGCCGGATCGTCAAAAATATCGTCTACGTCGCCCTCT
ACGCCGAGGCCGATCCCCGTCCGGTGCTCGTCTCGATCCGCGGCGACCGGCACATCAACGAGACCAAGCTCAAAAACCGC
CTCGACTGCCTGGACGTGCGCCTTGCCGACGAAGCGGAACTGGCCGCCTGGGTAGAGGTAAAACCGGGATTTGTCGGCCC
CGACGCGCCGATTGCCGGGGTGATTCGCCTGGCCGACCGCTCGGTGGACGGGCTGACCGATTTTTCCACCGGCTGCAACC
AGGACGACGTGCAGTGCGTCTGGGCCAACTGGGGCGAGAACGGATTGGTGCTACCCGAGGTGGCGGACCTCGACACTGCC
CAGGCGGGGGACCACTGCCACCTGGCACCGGAGGCGACCCTGCAGAGTGCCCGGGGCGTCGAGTTGGGCCATATTTTTAA
GCTCGGCACCAAGTACTCGCGGCCGATGCAGGTACTCTTCGCCGACGAGGCGGGCGAACTGCAGCCGGCTCTGATGGGTT
GCTACGGGGTCGGGGTCTCGCGGCTGCCCGCGGCGGTGGTCGAGCAGTCCCACGACAACGACGGGATCCTCTGGCCCATC
GCCATCGCTCCCTACCAGGTCGTGCTGGTGCCGGCAAACGTCGCTGTCGAAGCGCAGCGCCAGGCCGCCGAGGAACTCTA
TCGGTCCCTCACCGCGGCGGGGATCGACACGTTGCTCGACGACCGGCCGGAGCGGGCCGGCGTCAAATTCAAAGACGCCG
ATCTCATCGGTATTCCCCTCCGGGTCACCCTCGGCCGCGACCTCGAAGCCGGTCTGGTAGAAATCAAAGTGCGCGGCGGC
GGCGCGGCGGAAAAAGTGCCCTTAGCCGAAGCGCTTGCCCAGATCAAGGGGCTTATCGAGCGGTTGAACTCGGCCCAGCA
CTCGCGCTAG

Upstream 100 bases:

>100_bases
GCTCAGCACAACCAAACCTCCTCCACATGTAACATCAGCCTACAATGTATTACAGATGGACCCCATCCTAAGATAGAAAA
ACTGATTGACCGAGCCCGCC

Downstream 100 bases:

>100_bases
CGTAAACGAGCCACCCGTCTGGAGCGCTGTGATGCTGGTACCTTCGTATTTTTTGATCCTGGGAGGGTTTCTCGCCGTGC
TCACGGCAGGAGCAGCCCTG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MTRLSSSFFFTLREAPAEAVAVSHKLLLRAGFIRPLAGTAGLYAYGPLMQRVLQKVGRIVREEMDATGAQEALFCQLQPA
EIWKESGRWTVYTQDGTMFTLKDGQGEQAREYGLGPTHEEAVCDFVRASLNSYKQLPFHLYQVQTKFRNEKRPRFGLMRG
REFIMKDGYSFHATPESLDETYRAMYRAYTNMFRRCGLDFRAVEADSGAIGGSGSHEFMALCDIGEDTILYCDAAGYAAN
VEKAVSLVSDPEAIGPGSYAVKSTPGIRTVEQQAAMLGVPISRIVKNIVYVALYAEADPRPVLVSIRGDRHINETKLKNR
LDCLDVRLADEAELAAWVEVKPGFVGPDAPIAGVIRLADRSVDGLTDFSTGCNQDDVQCVWANWGENGLVLPEVADLDTA
QAGDHCHLAPEATLQSARGVELGHIFKLGTKYSRPMQVLFADEAGELQPALMGCYGVGVSRLPAAVVEQSHDNDGILWPI
AIAPYQVVLVPANVAVEAQRQAAEELYRSLTAAGIDTLLDDRPERAGVKFKDADLIGIPLRVTLGRDLEAGLVEIKVRGG
GAAEKVPLAEALAQIKGLIERLNSAQHSR

Sequences:

>Translated_589_residues
MTRLSSSFFFTLREAPAEAVAVSHKLLLRAGFIRPLAGTAGLYAYGPLMQRVLQKVGRIVREEMDATGAQEALFCQLQPA
EIWKESGRWTVYTQDGTMFTLKDGQGEQAREYGLGPTHEEAVCDFVRASLNSYKQLPFHLYQVQTKFRNEKRPRFGLMRG
REFIMKDGYSFHATPESLDETYRAMYRAYTNMFRRCGLDFRAVEADSGAIGGSGSHEFMALCDIGEDTILYCDAAGYAAN
VEKAVSLVSDPEAIGPGSYAVKSTPGIRTVEQQAAMLGVPISRIVKNIVYVALYAEADPRPVLVSIRGDRHINETKLKNR
LDCLDVRLADEAELAAWVEVKPGFVGPDAPIAGVIRLADRSVDGLTDFSTGCNQDDVQCVWANWGENGLVLPEVADLDTA
QAGDHCHLAPEATLQSARGVELGHIFKLGTKYSRPMQVLFADEAGELQPALMGCYGVGVSRLPAAVVEQSHDNDGILWPI
AIAPYQVVLVPANVAVEAQRQAAEELYRSLTAAGIDTLLDDRPERAGVKFKDADLIGIPLRVTLGRDLEAGLVEIKVRGG
GAAEKVPLAEALAQIKGLIERLNSAQHSR
>Mature_588_residues
TRLSSSFFFTLREAPAEAVAVSHKLLLRAGFIRPLAGTAGLYAYGPLMQRVLQKVGRIVREEMDATGAQEALFCQLQPAE
IWKESGRWTVYTQDGTMFTLKDGQGEQAREYGLGPTHEEAVCDFVRASLNSYKQLPFHLYQVQTKFRNEKRPRFGLMRGR
EFIMKDGYSFHATPESLDETYRAMYRAYTNMFRRCGLDFRAVEADSGAIGGSGSHEFMALCDIGEDTILYCDAAGYAANV
EKAVSLVSDPEAIGPGSYAVKSTPGIRTVEQQAAMLGVPISRIVKNIVYVALYAEADPRPVLVSIRGDRHINETKLKNRL
DCLDVRLADEAELAAWVEVKPGFVGPDAPIAGVIRLADRSVDGLTDFSTGCNQDDVQCVWANWGENGLVLPEVADLDTAQ
AGDHCHLAPEATLQSARGVELGHIFKLGTKYSRPMQVLFADEAGELQPALMGCYGVGVSRLPAAVVEQSHDNDGILWPIA
IAPYQVVLVPANVAVEAQRQAAEELYRSLTAAGIDTLLDDRPERAGVKFKDADLIGIPLRVTLGRDLEAGLVEIKVRGGG
AAEKVPLAEALAQIKGLIERLNSAQHSR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=249, Percent_Identity=41.3654618473896, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI1786392, Length=584, Percent_Identity=44.8630136986301, Blast_Score=470, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI115532348, Length=218, Percent_Identity=36.2385321100917, Blast_Score=132, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6320931, Length=553, Percent_Identity=29.8372513562387, Blast_Score=225, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24656200, Length=239, Percent_Identity=37.2384937238494, Blast_Score=158, Evalue=9e-39,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_GLOVI (Q7NHL0)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925471.1
- ProteinModelPortal:   Q7NHL0
- SMR:   Q7NHL0
- GeneID:   2600926
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll2525
- NMPDR:   fig|251221.1.peg.2525
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   GVIO251221:GLL2525-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 64303; Mature: 64172

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLSSSFFFTLREAPAEAVAVSHKLLLRAGFIRPLAGTAGLYAYGPLMQRVLQKVGRIV
CCCCCCCEEEEEHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
REEMDATGAQEALFCQLQPAEIWKESGRWTVYTQDGTMFTLKDGQGEQAREYGLGPTHEE
HHHHCCCCCCCEEEEECCHHHHHHCCCCEEEEECCCEEEEEECCCCCHHHHCCCCCCHHH
AVCDFVRASLNSYKQLPFHLYQVQTKFRNEKRPRFGLMRGREFIMKDGYSFHATPESLDE
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHCCHHHHHCCCEECCCHHHHHH
TYRAMYRAYTNMFRRCGLDFRAVEADSGAIGGSGSHEFMALCDIGEDTILYCDAAGYAAN
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCHHC
VEKAVSLVSDPEAIGPGSYAVKSTPGIRTVEQQAAMLGVPISRIVKNIVYVALYAEADPR
HHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCHHHHHHHHEEEEEEECCCCC
PVLVSIRGDRHINETKLKNRLDCLDVRLADEAELAAWVEVKPGFVGPDAPIAGVIRLADR
EEEEEECCCCCCCHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHCC
SVDGLTDFSTGCNQDDVQCVWANWGENGLVLPEVADLDTAQAGDHCHLAPEATLQSARGV
CCCCCCCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCCCCCCCEECCCHHHHHHCCCC
ELGHIFKLGTKYSRPMQVLFADEAGELQPALMGCYGVGVSRLPAAVVEQSHDNDGILWPI
CHHHHEECCCCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHCCHHHHHCCCCCCCEEEEE
AIAPYQVVLVPANVAVEAQRQAAEELYRSLTAAGIDTLLDDRPERAGVKFKDADLIGIPL
EECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEECCCEEEEEE
RVTLGRDLEAGLVEIKVRGGGAAEKVPLAEALAQIKGLIERLNSAQHSR
EEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRLSSSFFFTLREAPAEAVAVSHKLLLRAGFIRPLAGTAGLYAYGPLMQRVLQKVGRIV
CCCCCCEEEEEHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
REEMDATGAQEALFCQLQPAEIWKESGRWTVYTQDGTMFTLKDGQGEQAREYGLGPTHEE
HHHHCCCCCCCEEEEECCHHHHHHCCCCEEEEECCCEEEEEECCCCCHHHHCCCCCCHHH
AVCDFVRASLNSYKQLPFHLYQVQTKFRNEKRPRFGLMRGREFIMKDGYSFHATPESLDE
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHCCHHHHHCCCEECCCHHHHHH
TYRAMYRAYTNMFRRCGLDFRAVEADSGAIGGSGSHEFMALCDIGEDTILYCDAAGYAAN
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCHHC
VEKAVSLVSDPEAIGPGSYAVKSTPGIRTVEQQAAMLGVPISRIVKNIVYVALYAEADPR
HHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCHHHHHHHHEEEEEEECCCCC
PVLVSIRGDRHINETKLKNRLDCLDVRLADEAELAAWVEVKPGFVGPDAPIAGVIRLADR
EEEEEECCCCCCCHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHCC
SVDGLTDFSTGCNQDDVQCVWANWGENGLVLPEVADLDTAQAGDHCHLAPEATLQSARGV
CCCCCCCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCCCCCCCEECCCHHHHHHCCCC
ELGHIFKLGTKYSRPMQVLFADEAGELQPALMGCYGVGVSRLPAAVVEQSHDNDGILWPI
CHHHHEECCCCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHCCHHHHHCCCCCCCEEEEE
AIAPYQVVLVPANVAVEAQRQAAEELYRSLTAAGIDTLLDDRPERAGVKFKDADLIGIPL
EECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEECCCEEEEEE
RVTLGRDLEAGLVEIKVRGGGAAEKVPLAEALAQIKGLIERLNSAQHSR
EEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292