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The map label for this gene is gyrB [H]

Identifier: 37522075

GI number: 37522075

Start: 2663827

End: 2665761

Strand: Reverse

Name: gyrB [H]

Synonym: gvip344

Alternate gene names: 37522075

Gene position: 2665761-2663827 (Counterclockwise)

Preceding gene: 37522077

Following gene: 37522074

Centisome position: 57.22

GC content: 60.16

Gene sequence:

>1935_bases
ATGACCGAAACCTATGGAGCGGAGCAGATTCAGGTTCTCGAAGGCCTGGAGCATGTCCGCAAGCGCCCGGGCATGTACAT
CGGCTCCACGGGACCGCGCGGTCTGCATCACCTAGTTTACGAAATCGTCGATAACTCCGTCGACGAGGCTCTTGCCGGTC
ACTGCAAGCACATCGCCATCAGCCTGGGCGCCGACGGCTCCGCCACGATCACCGACGACGGGCGCGGCATACCGACCGAC
ACCCACCCGCGCACGGGCAAATCCACCATTGAGACGGTACTTACGGTGCTCGGTGCCGGCGGCAAGTTCGGCGGCGGCGG
CTACAAAGTCTCGGGTGGTCTGCACGGCGTCGGCGCGGCGGTGGTCAATGCCCTGTCGACCATCCTCACCGCGACGATCT
GGCGCGAGGGCAAGCAGTACATTCAACGCTTCCATCGCGGCATTCCCGAGGGCGGTCTTGCGGTGAGTTCCGACTCCCAG
AAGCGTCGCGGCACGATGATTTCGTTCTTGCCCGATCCCGAAATTTTTACCACCGGCGTCGATTTTGATTTCGACACGCT
CTTGAGTCGCTTTCGCGAACTGGCCTTTTTGAACGCGGGGGTCGAATTTCGCTTCAGCGATTTGCGCGGTGAGACCGAGC
GCACCGAAAGCTATATCTACGAAGGCGGCATCCGCGAGTACGTCAAGTACATGACCCGCGAGAAGGCTGCTTTGCACCCC
GATATCATCTTTATCAACCAGGAGCGCGACGGGGTAAGCGTCGAGTGCGCCCTACAGTGGAGCACCGACGTCTATAACGA
CAACGTGCTGGGTTTTGCCAACAACATCCGCACCATCGACGGCGGCACCCACCTCGAAGGTCTTAAAGCCGTCCTCACCC
GCACCTTCAACAGCTTCGCCCGCAAGGCCAACAAGCTCAAAGACGGTGACAAGAACCTCACCGGCGAGCACGTGCGGGAG
GGGCTGACCGCCGTGTTGAGCGTCAAAGTCCCCAACCCCGAATTTGAAGGCCAGACCAAGACCAAACTCGGCAACCCCGA
GGTGCGCGGCATCGTCGACGGCGTGATTTCCGAGAAACTCACCGAATACCTCGAATTTCACCCGGATGTGGTGGCTTCCA
TCCTCGAAAAGGCTCTGCAGTCGATGCAGGCCGAGGAAGCGGCCCGCAAAGCGCGCGACCTGGTCCGCCGCAAATCGGCC
CTCGAATCGTCCACCCTCCCCGGCAAACTCGCCGACTGCCAGAGCCGCGACCCCGCAGAATCGGAAGTCTTTCTAGTAGA
AGGAGATTCGGCGGGCGGGAGCGCCAAGCAAGGGCGGGATCGGCGATTCCAGGCGATTTTGCCGCTGCGGGGCAAAATCC
TCAATATCGAAAGAGCCGACGACCGAAAGATTTACGGCAACAACGAGATCCAGGCGATGATCACCGGTCTCGGCCTCGGC
CTCAAAACCGAAGAATTCGATGTCGGTCGGCTCAGATACCATCGCATCATTATCATGACCGACGCAGACGTCGACGGCGC
CCACATCCGCACGTTGCTGCTCACGTTCTTCTACCGCTACAAACGCGACTTGGTCGAGCAGGGCTACATTTACATCGCCC
AGCCGCCGTTGTACAAAATTTCGATCGGCGGTGGCCGCAATATCGACGTGCGCTACTGCTACTCCGAACAGGAAAAAGAG
GCGATCCTTCGCGGTCTGCGCGAGAACCAAAAGTATGAGCTCCAGCGCTTTAAGGGTCTAGGCGAGATGCAGGCGGATCA
GCTCTGGGAGACGACGATGAACCCCGAGACGCGCACCCTCAGGCAAGTGACCATCGAGGACGCCGCCGAGGCCGACCGCG
TCTTCAACATCCTGATGGGCGACCGCGTCGAACCCAGACGCGAATTTATCGAGACCTACGGTCCACGGCTGCAGATGGCC
GACCTGGACATTTAG

Upstream 100 bases:

>100_bases
ATGCGTGATCTGACCTCGCCCCGTTTTGACGCGCCTTTGGGCATAGCGGCACTAACGACCGTGGGCGATACGCTAGCTGG
CGCGAAGGAGCGCACGACGA

Downstream 100 bases:

>100_bases
GTTCGGTGTCAAGTGGATCAGACCCCGCCGGCCAGGTGCGCCGCACCCCGAAACAGGAGCGCGGTCGACAGCGCGTCGAG
CGGATTTTGCCGGCCGCCAC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 644; Mature: 643

Protein sequence:

>644_residues
MTETYGAEQIQVLEGLEHVRKRPGMYIGSTGPRGLHHLVYEIVDNSVDEALAGHCKHIAISLGADGSATITDDGRGIPTD
THPRTGKSTIETVLTVLGAGGKFGGGGYKVSGGLHGVGAAVVNALSTILTATIWREGKQYIQRFHRGIPEGGLAVSSDSQ
KRRGTMISFLPDPEIFTTGVDFDFDTLLSRFRELAFLNAGVEFRFSDLRGETERTESYIYEGGIREYVKYMTREKAALHP
DIIFINQERDGVSVECALQWSTDVYNDNVLGFANNIRTIDGGTHLEGLKAVLTRTFNSFARKANKLKDGDKNLTGEHVRE
GLTAVLSVKVPNPEFEGQTKTKLGNPEVRGIVDGVISEKLTEYLEFHPDVVASILEKALQSMQAEEAARKARDLVRRKSA
LESSTLPGKLADCQSRDPAESEVFLVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERADDRKIYGNNEIQAMITGLGLG
LKTEEFDVGRLRYHRIIIMTDADVDGAHIRTLLLTFFYRYKRDLVEQGYIYIAQPPLYKISIGGGRNIDVRYCYSEQEKE
AILRGLRENQKYELQRFKGLGEMQADQLWETTMNPETRTLRQVTIEDAAEADRVFNILMGDRVEPRREFIETYGPRLQMA
DLDI

Sequences:

>Translated_644_residues
MTETYGAEQIQVLEGLEHVRKRPGMYIGSTGPRGLHHLVYEIVDNSVDEALAGHCKHIAISLGADGSATITDDGRGIPTD
THPRTGKSTIETVLTVLGAGGKFGGGGYKVSGGLHGVGAAVVNALSTILTATIWREGKQYIQRFHRGIPEGGLAVSSDSQ
KRRGTMISFLPDPEIFTTGVDFDFDTLLSRFRELAFLNAGVEFRFSDLRGETERTESYIYEGGIREYVKYMTREKAALHP
DIIFINQERDGVSVECALQWSTDVYNDNVLGFANNIRTIDGGTHLEGLKAVLTRTFNSFARKANKLKDGDKNLTGEHVRE
GLTAVLSVKVPNPEFEGQTKTKLGNPEVRGIVDGVISEKLTEYLEFHPDVVASILEKALQSMQAEEAARKARDLVRRKSA
LESSTLPGKLADCQSRDPAESEVFLVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERADDRKIYGNNEIQAMITGLGLG
LKTEEFDVGRLRYHRIIIMTDADVDGAHIRTLLLTFFYRYKRDLVEQGYIYIAQPPLYKISIGGGRNIDVRYCYSEQEKE
AILRGLRENQKYELQRFKGLGEMQADQLWETTMNPETRTLRQVTIEDAAEADRVFNILMGDRVEPRREFIETYGPRLQMA
DLDI
>Mature_643_residues
TETYGAEQIQVLEGLEHVRKRPGMYIGSTGPRGLHHLVYEIVDNSVDEALAGHCKHIAISLGADGSATITDDGRGIPTDT
HPRTGKSTIETVLTVLGAGGKFGGGGYKVSGGLHGVGAAVVNALSTILTATIWREGKQYIQRFHRGIPEGGLAVSSDSQK
RRGTMISFLPDPEIFTTGVDFDFDTLLSRFRELAFLNAGVEFRFSDLRGETERTESYIYEGGIREYVKYMTREKAALHPD
IIFINQERDGVSVECALQWSTDVYNDNVLGFANNIRTIDGGTHLEGLKAVLTRTFNSFARKANKLKDGDKNLTGEHVREG
LTAVLSVKVPNPEFEGQTKTKLGNPEVRGIVDGVISEKLTEYLEFHPDVVASILEKALQSMQAEEAARKARDLVRRKSAL
ESSTLPGKLADCQSRDPAESEVFLVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERADDRKIYGNNEIQAMITGLGLGL
KTEEFDVGRLRYHRIIIMTDADVDGAHIRTLLLTFFYRYKRDLVEQGYIYIAQPPLYKISIGGGRNIDVRYCYSEQEKEA
ILRGLRENQKYELQRFKGLGEMQADQLWETTMNPETRTLRQVTIEDAAEADRVFNILMGDRVEPRREFIETYGPRLQMAD
LDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=613, Percent_Identity=25.7748776508972, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI19913408, Length=664, Percent_Identity=25.1506024096386, Blast_Score=134, Evalue=4e-31,
Organism=Escherichia coli, GI48994957, Length=544, Percent_Identity=52.9411764705882, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI1789408, Length=650, Percent_Identity=40.6153846153846, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI212645845, Length=536, Percent_Identity=24.6268656716418, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17535065, Length=620, Percent_Identity=25.6451612903226, Blast_Score=125, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=165, Percent_Identity=32.1212121212121, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=679, Percent_Identity=25.7731958762887, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17136538, Length=610, Percent_Identity=25.0819672131148, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71763; Mature: 71632

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETYGAEQIQVLEGLEHVRKRPGMYIGSTGPRGLHHLVYEIVDNSVDEALAGHCKHIAI
CCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCEEEEE
SLGADGSATITDDGRGIPTDTHPRTGKSTIETVLTVLGAGGKFGGGGYKVSGGLHGVGAA
EECCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHH
VVNALSTILTATIWREGKQYIQRFHRGIPEGGLAVSSDSQKRRGTMISFLPDPEIFTTGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHCCCEEEECCCCCEEEECC
DFDFDTLLSRFRELAFLNAGVEFRFSDLRGETERTESYIYEGGIREYVKYMTREKAALHP
CCCHHHHHHHHHHHHHHCCCCEEEEHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
DIIFINQERDGVSVECALQWSTDVYNDNVLGFANNIRTIDGGTHLEGLKAVLTRTFNSFA
CEEEEECCCCCCEEEEEEEECCCCCCCCEEEECCCCEECCCCCHHHHHHHHHHHHHHHHH
RKANKLKDGDKNLTGEHVREGLTAVLSVKVPNPEFEGQTKTKLGNPEVRGIVDGVISEKL
HHHHHCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
TEYLEFHPDVVASILEKALQSMQAEEAARKARDLVRRKSALESSTLPGKLADCQSRDPAE
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCC
SEVFLVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERADDRKIYGNNEIQAMITGLGLG
CEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCEECCCCHHEEEEECCCCC
LKTEEFDVGRLRYHRIIIMTDADVDGAHIRTLLLTFFYRYKRDLVEQGYIYIAQPPLYKI
CCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEE
SIGGGRNIDVRYCYSEQEKEAILRGLRENQKYELQRFKGLGEMQADQLWETTMNPETRTL
ECCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHCCCCCHHHH
RQVTIEDAAEADRVFNILMGDRVEPRREFIETYGPRLQMADLDI
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCC
>Mature Secondary Structure 
TETYGAEQIQVLEGLEHVRKRPGMYIGSTGPRGLHHLVYEIVDNSVDEALAGHCKHIAI
CCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCEEEEE
SLGADGSATITDDGRGIPTDTHPRTGKSTIETVLTVLGAGGKFGGGGYKVSGGLHGVGAA
EECCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHH
VVNALSTILTATIWREGKQYIQRFHRGIPEGGLAVSSDSQKRRGTMISFLPDPEIFTTGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHCCCEEEECCCCCEEEECC
DFDFDTLLSRFRELAFLNAGVEFRFSDLRGETERTESYIYEGGIREYVKYMTREKAALHP
CCCHHHHHHHHHHHHHHCCCCEEEEHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
DIIFINQERDGVSVECALQWSTDVYNDNVLGFANNIRTIDGGTHLEGLKAVLTRTFNSFA
CEEEEECCCCCCEEEEEEEECCCCCCCCEEEECCCCEECCCCCHHHHHHHHHHHHHHHHH
RKANKLKDGDKNLTGEHVREGLTAVLSVKVPNPEFEGQTKTKLGNPEVRGIVDGVISEKL
HHHHHCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
TEYLEFHPDVVASILEKALQSMQAEEAARKARDLVRRKSALESSTLPGKLADCQSRDPAE
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCC
SEVFLVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERADDRKIYGNNEIQAMITGLGLG
CEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCEECCCCHHEEEEECCCCC
LKTEEFDVGRLRYHRIIIMTDADVDGAHIRTLLLTFFYRYKRDLVEQGYIYIAQPPLYKI
CCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEE
SIGGGRNIDVRYCYSEQEKEAILRGLRENQKYELQRFKGLGEMQADQLWETTMNPETRTL
ECCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHCCCCCHHHH
RQVTIEDAAEADRVFNILMGDRVEPRREFIETYGPRLQMADLDI
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]