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The map label for this gene is recF

Identifier: 37521974

GI number: 37521974

Start: 2563502

End: 2564629

Strand: Reverse

Name: recF

Synonym: gvip337

Alternate gene names: 37521974

Gene position: 2564629-2563502 (Counterclockwise)

Preceding gene: 37521979

Following gene: 37521970

Centisome position: 55.05

GC content: 64.01

Gene sequence:

>1128_bases
GTGTTCCTAAGATCCGTTCAGTTGCACGATTTTCGCAACTACGCCGAAGCCGACCTCGAACTGACCAGCCCCAAGACGAT
CCTGGTGGGCGACAACGCCCAGGGCAAATCCAACCTGCTGGAGGCGGTGCAGTTGTTGGCCACGGGGCGCTCGACCCGGG
CTCTGCGCGACCGGGAACTGATCGCCCGGGGCAAAGAGCAGGCGCGGGTGGCAGCAACGGTCGAGCGGCTCGGAGATACG
GTCGAGTTGGAGATGATCCTGCGGGCGGGCAAACGGCGCACTGTGCGTGTGGGGGGCGAGACGCGCCGCACCCAGGTGGA
GGCGCTGGGGTATTTGCACTGTGTCTCTTTTTCGAGTCTCGATCTCGATCTGGTGCGCGGAGCGCCCGAGACGCGCCGCG
ACTGGCTCGACGGCATCTTGCTGCAACTGGAGCCGGTCTACACCAATGTGCTTGCCCAGTTCGTGCAGGCGCTCCACCAG
CGCAACGCCCTGCTGCGCAGTACAGAGCTGTCCCCGGACGCCCTCGCCGAACAGTTGCCCTGCTGGGACGATTTGCTGGT
GCGCGCCGCTACACCCGTGATGCGCCGCCGTCACCGCCTCATCGAACGGCTCGCCCCCCTTGCCCGGCGCTGGCACGGCT
CGATTAGCGGCGGACGCGAGACGTTTGCGGTGCGCTACCAGCCGCAAATCAGCTTCGAGCAAGAGGATGCCCAGAGTGTC
CAGCAGGCATTGCAGGAGTTGCTCAAAGAAAAACGCACCCTCGAAGGGCGCCGCGGCACCAGCCTGGTGGGACCGCACCG
CGACGAAGTGGATCTCAGTATCGATGAAATCCCGGCCCGCCAGTTCGGTTCCCAGGGGCAACAGCGTACCCTGGTGCTCG
CCCTCAAGCTGGCGGAACTGGAGTTGCTTGAGCAGGTGACCGGCGAGGTGCCTTTGCTGTTGCTCGACGATGTCCTTGCT
GAACTGGATCTGCACCGTCAGGACCAGCTGCTGGGAGCTATCCAGGAGCGGGTGCAGACGATTGTGACCACCACCCATCT
GAGTTTGTTTGATAGTCAGTGGCTACAGTCGGCGACGGTGCTCACGATCGAAAAGGGGAGGATTGGCTCCCCCCCTGCAC
CCGCTTGA

Upstream 100 bases:

>100_bases
TGGGGTGCGTCGTACTCCCGGATCAAGACGGCGCAAAACTTCCGGCGGGCAAACCGGTGCGCTTTAGACGATGCTATAGA
GAAGAGCCGCTACGACAATT

Downstream 100 bases:

>100_bases
AGGGGGAGAGCTACTGCAGGGAGAAGGTGACCGGGATGCGCACCCACGCCCCCACCGACTCCTCCCCCCGCCGCGCCGGC
TCGAACTTCGTGTTGCGCAC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MFLRSVQLHDFRNYAEADLELTSPKTILVGDNAQGKSNLLEAVQLLATGRSTRALRDRELIARGKEQARVAATVERLGDT
VELEMILRAGKRRTVRVGGETRRTQVEALGYLHCVSFSSLDLDLVRGAPETRRDWLDGILLQLEPVYTNVLAQFVQALHQ
RNALLRSTELSPDALAEQLPCWDDLLVRAATPVMRRRHRLIERLAPLARRWHGSISGGRETFAVRYQPQISFEQEDAQSV
QQALQELLKEKRTLEGRRGTSLVGPHRDEVDLSIDEIPARQFGSQGQQRTLVLALKLAELELLEQVTGEVPLLLLDDVLA
ELDLHRQDQLLGAIQERVQTIVTTTHLSLFDSQWLQSATVLTIEKGRIGSPPAPA

Sequences:

>Translated_375_residues
MFLRSVQLHDFRNYAEADLELTSPKTILVGDNAQGKSNLLEAVQLLATGRSTRALRDRELIARGKEQARVAATVERLGDT
VELEMILRAGKRRTVRVGGETRRTQVEALGYLHCVSFSSLDLDLVRGAPETRRDWLDGILLQLEPVYTNVLAQFVQALHQ
RNALLRSTELSPDALAEQLPCWDDLLVRAATPVMRRRHRLIERLAPLARRWHGSISGGRETFAVRYQPQISFEQEDAQSV
QQALQELLKEKRTLEGRRGTSLVGPHRDEVDLSIDEIPARQFGSQGQQRTLVLALKLAELELLEQVTGEVPLLLLDDVLA
ELDLHRQDQLLGAIQERVQTIVTTTHLSLFDSQWLQSATVLTIEKGRIGSPPAPA
>Mature_375_residues
MFLRSVQLHDFRNYAEADLELTSPKTILVGDNAQGKSNLLEAVQLLATGRSTRALRDRELIARGKEQARVAATVERLGDT
VELEMILRAGKRRTVRVGGETRRTQVEALGYLHCVSFSSLDLDLVRGAPETRRDWLDGILLQLEPVYTNVLAQFVQALHQ
RNALLRSTELSPDALAEQLPCWDDLLVRAATPVMRRRHRLIERLAPLARRWHGSISGGRETFAVRYQPQISFEQEDAQSV
QQALQELLKEKRTLEGRRGTSLVGPHRDEVDLSIDEIPARQFGSQGQQRTLVLALKLAELELLEQVTGEVPLLLLDDVLA
ELDLHRQDQLLGAIQERVQTIVTTTHLSLFDSQWLQSATVLTIEKGRIGSPPAPA

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=376, Percent_Identity=27.3936170212766, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_GLOVI (Q7NHY0)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925351.1
- ProteinModelPortal:   Q7NHY0
- SMR:   Q7NHY0
- GeneID:   2600806
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.2405
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   GVIO251221:GLL2405-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42092; Mature: 42092

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLRSVQLHDFRNYAEADLELTSPKTILVGDNAQGKSNLLEAVQLLATGRSTRALRDREL
CCCCCCHHHHHHHHHHCCEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
IARGKEQARVAATVERLGDTVELEMILRAGKRRTVRVGGETRRTQVEALGYLHCVSFSSL
HHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCC
DLDLVRGAPETRRDWLDGILLQLEPVYTNVLAQFVQALHQRNALLRSTELSPDALAEQLP
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHCC
CWDDLLVRAATPVMRRRHRLIERLAPLARRWHGSISGGRETFAVRYQPQISFEQEDAQSV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHH
QQALQELLKEKRTLEGRRGTSLVGPHRDEVDLSIDEIPARQFGSQGQQRTLVLALKLAEL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHCCCCCCEEEHHHHHHHHH
ELLEQVTGEVPLLLLDDVLAELDLHRQDQLLGAIQERVQTIVTTTHLSLFDSQWLQSATV
HHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTIEKGRIGSPPAPA
EEEECCCCCCCCCCC
>Mature Secondary Structure
MFLRSVQLHDFRNYAEADLELTSPKTILVGDNAQGKSNLLEAVQLLATGRSTRALRDREL
CCCCCCHHHHHHHHHHCCEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
IARGKEQARVAATVERLGDTVELEMILRAGKRRTVRVGGETRRTQVEALGYLHCVSFSSL
HHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCC
DLDLVRGAPETRRDWLDGILLQLEPVYTNVLAQFVQALHQRNALLRSTELSPDALAEQLP
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHCC
CWDDLLVRAATPVMRRRHRLIERLAPLARRWHGSISGGRETFAVRYQPQISFEQEDAQSV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHH
QQALQELLKEKRTLEGRRGTSLVGPHRDEVDLSIDEIPARQFGSQGQQRTLVLALKLAEL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHCCCCCCEEEHHHHHHHHH
ELLEQVTGEVPLLLLDDVLAELDLHRQDQLLGAIQERVQTIVTTTHLSLFDSQWLQSATV
HHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTIEKGRIGSPPAPA
EEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292