Click here to switch to the map view.

The map label for this gene is livK [C]

Identifier: 37521922

GI number: 37521922

Start: 2515233

End: 2516336

Strand: Direct

Name: livK [C]

Synonym: glr2353

Alternate gene names: 37521922

Gene position: 2515233-2516336 (Clockwise)

Preceding gene: 37521916

Following gene: 37521923

Centisome position: 53.99

GC content: 62.41

Gene sequence:

>1104_bases
ATGAAAAGAAGAGAATTTGCCGCTGGAGCGGCGCTGCTCACCCTATTGGGAGCCTGCGGCAGCTCAGACAAAGTGTATAT
AGGCGTTGCGGGTCCGCTCTCGGGTCCCCAGACGGTCCAGGGAGAATACATCCTCAAGGCGGTCGAACTGGCGGTCGAAC
AGGTCAACGTCAAAGGGGGCGTCAAGGGCAAGCAGATCGAAGTGGTCACCGGCGACGATCAGGCCAAACCCCTGGACGCC
ACCAGCGTGGCGCGCAAGCTCGCTTCGACCGAAGGGGTGATGGGAGTGGTCGGCCACTTCAACTCCGGCTGTTCGATTCC
GGCCTCGTCGATCTACAACCAGGGCAACCTGGTGATGATCACCCCCGGCAGCACCAATCCCGCCCTCACCGAAAACGGCC
TCAAGAACGTCTACCGCATCGTCGGCCGCGACGATCAGCAGGGGGCGGTCGACGGCGATTTTGCGCTTCGCTCCCTTCAG
GCCCAGCGCGTCGCCATCCTGCACGACAAGACCCCCTACGGCCAGGGACTCGCTTCGTTTTTCCGCGAGACGGTCGAAAC
AGGCGGGGCGGAGGTGACCTTCTTCGAAGGGATCACCCAGGGGGACCGCGATTTTCGGGCGGTGCTCACCCGCATCAAGG
GCATCGACCCCGACACGATCTTCTACGGCGGGGTGTTCGTCGAGGCGGGTCTGGTGGTGAGCCAGGCGCGTGAGCTGGGT
ATCAAGTCGCGCTTTATCAGCGGCGACGGCACCAAAGAGCAGAGCTTCATCGACATCGTCGGGAAGAACGCGGGCGACAT
CTACATCAGCGGTCCGGCCCTGGTCAACAACGCCGCCTTCGTGGACGCCTACATGGACAAGTACAACAACGAAGAGCCCG
GTCCCTTTTCACCCTACGCCTACGACGCGGCGCGCATCTTGATCGCCGCCATGGAAAGTGCCCCCGAGTTGACCCGGGAG
GCGGTAGCTGCAGAGGTGCGCAAGCTCAAAAATTTCGCGGGTCTGGCCGGTTCTATCACCTTCGACGCCAAGGGCGACCG
CGACAAGGCCCCCTTCGACATCTTCGTGATCAAAGACGGCGAGTTCGTGCCGTACAGGGCGTAG

Upstream 100 bases:

>100_bases
GCGGGCACCCTGGGAAGCAGGGAGTTGGCCGAATTCGTTCAACTTTTGTCCGAGCGCTGTAGTATGGTGCCGAAACCTGC
CCCTACCAGTCTTTGAAACC

Downstream 100 bases:

>100_bases
TCTTTTTTTATGGACCGCCCCAAACTCGTCGCCATCGTCACCGGTGCTCTGGCTCTGGCCCTGGGTATCGGTTATCTCGT
GCTGGTGCAGTTATTAGACC

Product: periplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKRREFAAGAALLTLLGACGSSDKVYIGVAGPLSGPQTVQGEYILKAVELAVEQVNVKGGVKGKQIEVVTGDDQAKPLDA
TSVARKLASTEGVMGVVGHFNSGCSIPASSIYNQGNLVMITPGSTNPALTENGLKNVYRIVGRDDQQGAVDGDFALRSLQ
AQRVAILHDKTPYGQGLASFFRETVETGGAEVTFFEGITQGDRDFRAVLTRIKGIDPDTIFYGGVFVEAGLVVSQARELG
IKSRFISGDGTKEQSFIDIVGKNAGDIYISGPALVNNAAFVDAYMDKYNNEEPGPFSPYAYDAARILIAAMESAPELTRE
AVAAEVRKLKNFAGLAGSITFDAKGDRDKAPFDIFVIKDGEFVPYRA

Sequences:

>Translated_367_residues
MKRREFAAGAALLTLLGACGSSDKVYIGVAGPLSGPQTVQGEYILKAVELAVEQVNVKGGVKGKQIEVVTGDDQAKPLDA
TSVARKLASTEGVMGVVGHFNSGCSIPASSIYNQGNLVMITPGSTNPALTENGLKNVYRIVGRDDQQGAVDGDFALRSLQ
AQRVAILHDKTPYGQGLASFFRETVETGGAEVTFFEGITQGDRDFRAVLTRIKGIDPDTIFYGGVFVEAGLVVSQARELG
IKSRFISGDGTKEQSFIDIVGKNAGDIYISGPALVNNAAFVDAYMDKYNNEEPGPFSPYAYDAARILIAAMESAPELTRE
AVAAEVRKLKNFAGLAGSITFDAKGDRDKAPFDIFVIKDGEFVPYRA
>Mature_367_residues
MKRREFAAGAALLTLLGACGSSDKVYIGVAGPLSGPQTVQGEYILKAVELAVEQVNVKGGVKGKQIEVVTGDDQAKPLDA
TSVARKLASTEGVMGVVGHFNSGCSIPASSIYNQGNLVMITPGSTNPALTENGLKNVYRIVGRDDQQGAVDGDFALRSLQ
AQRVAILHDKTPYGQGLASFFRETVETGGAEVTFFEGITQGDRDFRAVLTRIKGIDPDTIFYGGVFVEAGLVVSQARELG
IKSRFISGDGTKEQSFIDIVGKNAGDIYISGPALVNNAAFVDAYMDKYNNEEPGPFSPYAYDAARILIAAMESAPELTRE
AVAAEVRKLKNFAGLAGSITFDAKGDRDKAPFDIFVIKDGEFVPYRA

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=363, Percent_Identity=33.6088154269972, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI48994941, Length=360, Percent_Identity=33.0555555555556, Blast_Score=192, Evalue=2e-50,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39025; Mature: 39025

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRREFAAGAALLTLLGACGSSDKVYIGVAGPLSGPQTVQGEYILKAVELAVEQVNVKGG
CCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
VKGKQIEVVTGDDQAKPLDATSVARKLASTEGVMGVVGHFNSGCSIPASSIYNQGNLVMI
CCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCCCCEEEE
TPGSTNPALTENGLKNVYRIVGRDDQQGAVDGDFALRSLQAQRVAILHDKTPYGQGLASF
ECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCHHHHHH
FRETVETGGAEVTFFEGITQGDRDFRAVLTRIKGIDPDTIFYGGVFVEAGLVVSQARELG
HHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHCC
IKSRFISGDGTKEQSFIDIVGKNAGDIYISGPALVNNAAFVDAYMDKYNNEEPGPFSPYA
CHHEEECCCCCCCCCEEEEECCCCCEEEEECCHHCCCHHHHHHHHHHCCCCCCCCCCCCH
YDAARILIAAMESAPELTREAVAAEVRKLKNFAGLAGSITFDAKGDRDKAPFDIFVIKDG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEEECC
EFVPYRA
CCCCCCC
>Mature Secondary Structure
MKRREFAAGAALLTLLGACGSSDKVYIGVAGPLSGPQTVQGEYILKAVELAVEQVNVKGG
CCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
VKGKQIEVVTGDDQAKPLDATSVARKLASTEGVMGVVGHFNSGCSIPASSIYNQGNLVMI
CCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCCCCEEEE
TPGSTNPALTENGLKNVYRIVGRDDQQGAVDGDFALRSLQAQRVAILHDKTPYGQGLASF
ECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCHHHHHH
FRETVETGGAEVTFFEGITQGDRDFRAVLTRIKGIDPDTIFYGGVFVEAGLVVSQARELG
HHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHCC
IKSRFISGDGTKEQSFIDIVGKNAGDIYISGPALVNNAAFVDAYMDKYNNEEPGPFSPYA
CHHEEECCCCCCCCCEEEEECCCCCEEEEECCHHCCCHHHHHHHHHHCCCCCCCCCCCCH
YDAARILIAAMESAPELTREAVAAEVRKLKNFAGLAGSITFDAKGDRDKAPFDIFVIKDG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEEECC
EFVPYRA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA