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The map label for this gene is leuA

Identifier: 37521859

GI number: 37521859

Start: 2452977

End: 2454593

Strand: Reverse

Name: leuA

Synonym: gvip311

Alternate gene names: 37521859

Gene position: 2454593-2452977 (Counterclockwise)

Preceding gene: 37521860

Following gene: 37521858

Centisome position: 52.68

GC content: 64.81

Gene sequence:

>1617_bases
ATGTTTCAGGACCGCTTGATTATCTTCGACACCACCCTTAGAGACGGCGAGCAGTCGCCGGGGGCCACGCTCAACGCCGA
CGAAAAGGTGGAGATTGCCCGGCAGCTTGCCCGACTCGGTGTCGATGTGATCGAGGCCGGTTTTGCCTACGCCAGCCCCG
GGGATTTTGAGGCGGTCGAGCGGGTGGCCCGCACGGTGGGTACCGAGGACGGGCCGGTAATCTGCAGTCTGGCGCGGGCC
ATCCGCTCAGACATTCAAGCGGCGGCCGAGGCGATCCGCCCCGCCGCCCGCGGTCGTATCCACACGTTCATCTCGACTTC
GGACATTCACCTGGAGCACCAGTTGCGCAAAAGCCGCGCCGAGGTGCTCGCCATCGCCGCCGAGATGGTGGCCTTTGCCA
AGGGTTTCGTGGACGATGTCGAATTTTCACCGATGGACGCCGGCCGCTCCGCCCCGGAGTATCTCTACCGGGTGCTCGAA
GCGGCAATCGCAGCCGGGGCGACGACCGTCAACATCCCCGACACGGTGGGCTATCTGACGCCCGCAGAATTCGGCGGCCT
CATCCGCGGGATCACCCAGAACGTGCGGGGCATCGAGCGGGCGGTGATTTCGGTGCACTGCCACAACGACCTGGGTCTGG
CGGTGGCCAATTCCCTCGCGGCCATCGAGAACGGTGCCCGTCAGATCGAGTGCACGGTGAACGGGATCGGCGAGCGGGCG
GGCAACTGTTCTCTCGAAGAAATCGTCATGGCCCTGCACGTGCGCCGCCAGTTCTTCAACCCGATCTTCGGCCGTCCGGC
CGATTCGACCGTACCCTTGAGTACCATCGACACCCGCCAGATCTACAAAAGTTCGCGGCTGGTCTCCCACCTGACCGGCA
TGCTCGTCCAGCCCAACAAGGCGATCGTCGGGGCGAACGCCTTTGCCCACGAGTCGGGCATCCATCAGGACGGCGTGCTC
AAAAACCGGCTCACCTACGAGATCATGGACGCCGAGACAGTCGGGGTCAACGAAAACCGCATCGTCCTGGGCAAGCACTC
GGGCCGCAACGCCTTTCGCACCCGATTGGTCGAGTTGGGCTACGAACTGGGCGACGCCGATCTCAACCGTGCCTTCTTGC
GCTTTAAAGAACTGGCGGACAAGAAAAAGACCGTGAGCGACTGGGACATCGAGGCCGTCATCTCCGATGAAATTCGCCTC
ATCCCCGAAGCCTACCGCCTGGAGCAGGTGCAGGTGAGCTGCGGTGAGCCGGGACTGCCCACCGCCACCGTCCGCCTCAC
CGGCCCCGACGGCGTCGAGCGCGTCGATGCGGCCGTGGGCACCGGCCCGGTGGACGCGGTCTACAAGGCGATCAACCGCC
TTATCGAACTACCCAACGAACTGATCGAATTTTCGGTGCAGTCGGTGACGGCGGGCATCGATGCGATGGGCGAAGTGACC
ATCCGCGTGCGCCAGGACGGACGCACCTTCAGCGGCCACGCGGCCAACACCGATATCATCGTCGCCTCCGCCCGCGCCTA
CCTCAACGCCCTCAACAAGCTCCACTTCGCTCTGGCCCACCCCACCCACTCCGGCGGCGCCCTCGCCCACCCCGACGCCG
CCGCCCAGAAACTCTGA

Upstream 100 bases:

>100_bases
GACCCCTAAATCGTAGGCACTTCCGGACCGGTTCTGCCTCTCGGATGCATCGGCAAGCAAGATAGAATGGCTGACGTTTC
GCCTGAGAGGCTACAACGCA

Downstream 100 bases:

>100_bases
TGGTCGCCCTCTCCCGCCGCCACTTTGTCAGCTCAAGCCTCGCGGGGGCGGCGGCACTGTGGCTGCCTGCCGGTGCGCGG
GCCGCCGCCTGCCCGCGCAC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MFQDRLIIFDTTLRDGEQSPGATLNADEKVEIARQLARLGVDVIEAGFAYASPGDFEAVERVARTVGTEDGPVICSLARA
IRSDIQAAAEAIRPAARGRIHTFISTSDIHLEHQLRKSRAEVLAIAAEMVAFAKGFVDDVEFSPMDAGRSAPEYLYRVLE
AAIAAGATTVNIPDTVGYLTPAEFGGLIRGITQNVRGIERAVISVHCHNDLGLAVANSLAAIENGARQIECTVNGIGERA
GNCSLEEIVMALHVRRQFFNPIFGRPADSTVPLSTIDTRQIYKSSRLVSHLTGMLVQPNKAIVGANAFAHESGIHQDGVL
KNRLTYEIMDAETVGVNENRIVLGKHSGRNAFRTRLVELGYELGDADLNRAFLRFKELADKKKTVSDWDIEAVISDEIRL
IPEAYRLEQVQVSCGEPGLPTATVRLTGPDGVERVDAAVGTGPVDAVYKAINRLIELPNELIEFSVQSVTAGIDAMGEVT
IRVRQDGRTFSGHAANTDIIVASARAYLNALNKLHFALAHPTHSGGALAHPDAAAQKL

Sequences:

>Translated_538_residues
MFQDRLIIFDTTLRDGEQSPGATLNADEKVEIARQLARLGVDVIEAGFAYASPGDFEAVERVARTVGTEDGPVICSLARA
IRSDIQAAAEAIRPAARGRIHTFISTSDIHLEHQLRKSRAEVLAIAAEMVAFAKGFVDDVEFSPMDAGRSAPEYLYRVLE
AAIAAGATTVNIPDTVGYLTPAEFGGLIRGITQNVRGIERAVISVHCHNDLGLAVANSLAAIENGARQIECTVNGIGERA
GNCSLEEIVMALHVRRQFFNPIFGRPADSTVPLSTIDTRQIYKSSRLVSHLTGMLVQPNKAIVGANAFAHESGIHQDGVL
KNRLTYEIMDAETVGVNENRIVLGKHSGRNAFRTRLVELGYELGDADLNRAFLRFKELADKKKTVSDWDIEAVISDEIRL
IPEAYRLEQVQVSCGEPGLPTATVRLTGPDGVERVDAAVGTGPVDAVYKAINRLIELPNELIEFSVQSVTAGIDAMGEVT
IRVRQDGRTFSGHAANTDIIVASARAYLNALNKLHFALAHPTHSGGALAHPDAAAQKL
>Mature_538_residues
MFQDRLIIFDTTLRDGEQSPGATLNADEKVEIARQLARLGVDVIEAGFAYASPGDFEAVERVARTVGTEDGPVICSLARA
IRSDIQAAAEAIRPAARGRIHTFISTSDIHLEHQLRKSRAEVLAIAAEMVAFAKGFVDDVEFSPMDAGRSAPEYLYRVLE
AAIAAGATTVNIPDTVGYLTPAEFGGLIRGITQNVRGIERAVISVHCHNDLGLAVANSLAAIENGARQIECTVNGIGERA
GNCSLEEIVMALHVRRQFFNPIFGRPADSTVPLSTIDTRQIYKSSRLVSHLTGMLVQPNKAIVGANAFAHESGIHQDGVL
KNRLTYEIMDAETVGVNENRIVLGKHSGRNAFRTRLVELGYELGDADLNRAFLRFKELADKKKTVSDWDIEAVISDEIRL
IPEAYRLEQVQVSCGEPGLPTATVRLTGPDGVERVDAAVGTGPVDAVYKAINRLIELPNELIEFSVQSVTAGIDAMGEVT
IRVRQDGRTFSGHAANTDIIVASARAYLNALNKLHFALAHPTHSGGALAHPDAAAQKL

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=511, Percent_Identity=48.3365949119374, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6320071, Length=395, Percent_Identity=30.126582278481, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6320019, Length=395, Percent_Identity=29.620253164557, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324682, Length=333, Percent_Identity=27.9279279279279, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324225, Length=263, Percent_Identity=29.277566539924, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24582381, Length=268, Percent_Identity=27.2388059701493, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_GLOVI (Q7NI93)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925236.1
- ProteinModelPortal:   Q7NI93
- SMR:   Q7NI93
- GeneID:   2600692
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.2290
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   GVIO251221:GLL2290-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 58035; Mature: 58035

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQDRLIIFDTTLRDGEQSPGATLNADEKVEIARQLARLGVDVIEAGFAYASPGDFEAVE
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHH
RVARTVGTEDGPVICSLARAIRSDIQAAAEAIRPAARGRIHTFISTSDIHLEHQLRKSRA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
EVLAIAAEMVAFAKGFVDDVEFSPMDAGRSAPEYLYRVLEAAIAAGATTVNIPDTVGYLT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCC
PAEFGGLIRGITQNVRGIERAVISVHCHNDLGLAVANSLAAIENGARQIECTVNGIGERA
HHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCC
GNCSLEEIVMALHVRRQFFNPIFGRPADSTVPLSTIDTRQIYKSSRLVSHLTGMLVQPNK
CCCCHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCC
AIVGANAFAHESGIHQDGVLKNRLTYEIMDAETVGVNENRIVLGKHSGRNAFRTRLVELG
EEEECCHHHHCCCCCCCCCHHCCCEEEEECCEEECCCCCEEEEECCCCCHHHHHHHHHHH
YELGDADLNRAFLRFKELADKKKTVSDWDIEAVISDEIRLIPEAYRLEQVQVSCGEPGLP
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHCCHHHHHEEEEEECCCCCCC
TATVRLTGPDGVERVDAAVGTGPVDAVYKAINRLIELPNELIEFSVQSVTAGIDAMGEVT
EEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEE
IRVRQDGRTFSGHAANTDIIVASARAYLNALNKLHFALAHPTHSGGALAHPDAAAQKL
EEEEECCCEECCCCCCCCEEEEHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MFQDRLIIFDTTLRDGEQSPGATLNADEKVEIARQLARLGVDVIEAGFAYASPGDFEAVE
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHH
RVARTVGTEDGPVICSLARAIRSDIQAAAEAIRPAARGRIHTFISTSDIHLEHQLRKSRA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
EVLAIAAEMVAFAKGFVDDVEFSPMDAGRSAPEYLYRVLEAAIAAGATTVNIPDTVGYLT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCC
PAEFGGLIRGITQNVRGIERAVISVHCHNDLGLAVANSLAAIENGARQIECTVNGIGERA
HHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCC
GNCSLEEIVMALHVRRQFFNPIFGRPADSTVPLSTIDTRQIYKSSRLVSHLTGMLVQPNK
CCCCHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCC
AIVGANAFAHESGIHQDGVLKNRLTYEIMDAETVGVNENRIVLGKHSGRNAFRTRLVELG
EEEECCHHHHCCCCCCCCCHHCCCEEEEECCEEECCCCCEEEEECCCCCHHHHHHHHHHH
YELGDADLNRAFLRFKELADKKKTVSDWDIEAVISDEIRLIPEAYRLEQVQVSCGEPGLP
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHCCHHHHHEEEEEECCCCCCC
TATVRLTGPDGVERVDAAVGTGPVDAVYKAINRLIELPNELIEFSVQSVTAGIDAMGEVT
EEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEE
IRVRQDGRTFSGHAANTDIIVASARAYLNALNKLHFALAHPTHSGGALAHPDAAAQKL
EEEEECCCEECCCCCCCCEEEEHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292