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The map label for this gene is ntrB [H]

Identifier: 37521781

GI number: 37521781

Start: 2366778

End: 2368205

Strand: Direct

Name: ntrB [H]

Synonym: glr2212

Alternate gene names: 37521781

Gene position: 2366778-2368205 (Clockwise)

Preceding gene: 37521740

Following gene: 37521782

Centisome position: 50.8

GC content: 62.18

Gene sequence:

>1428_bases
GTGGGGAGCTTCGCCGCCATGCTCCGCGTCGACGAGTTGCTGCGCCTGGAGGTCTTTGCGAAACTGCCGCCTTCCCGGCT
GGAGTGGGTGTGTGGGCGTGTCCGGCCCGTAGTGCTGTGCGCCGGTGAAACGCTTGTCAAAGAGGGCGATCCGCCGCGGG
GATTTTTCATCCTGGCTGAGGGCAAGCTGGGCATCTGGCGCCAGAGCGACGGTGTGGAGATGCCCATCGGCCGGCACGAG
GCGCCGGGCTTCGTGGGGGAGGTGCCGGTGCTGACCGACGAGCCGATGCTGGTGAGCGTCCGGGCGCTCACCGACTGCCG
CGTCTACGAACTGCCCGCCAGTGACTTTTTAGAACTCATTCACACCTGCCGCGATGTCGAGCGGTTGATCTTCCGGGAGG
TGCACCACCGCCTGCGCGGGTTGGAATCTTTCGTGCGCACCCGCGAGAAGATGGCGGCTTTGGGCACGCTGTCGGCGGGT
CTGGCCCACGAACTGAACAATCCGGCGGCAGCCCTGGTGCGCGTACTCAACAACGTCGCCCCGGCCATCGTCGAGTTGCA
GCGGATGAACCTGATTTATGGCCAGAGCAACCCTGATCCCAAGCACTCCGAGCAGTGGCTGCAGGTGCGCGACGTGGGCT
ACGAAGCCATCGCCCATCCCACCGAGGACGCATCGGTGCGGGACGATCTCGAAGAACAACTGCTCGCCTGGCTGGAGGAC
TATGGGGTCGAAGACGCCTGGAAGGTGGCCGGGCCGCTGGCGTTGGGTCGGGTGACACCCGAGACGCTTGCTGTGCTGAT
CGAACCCTGGCGGGATCATCCCGGCGAACTCAGGGACATGGGCATTCGCTGGCTGGCTCTTTCATTTGACGTCATGGAGA
TGATCCAGAGCGGTCTGCGCGGCTCGCGGCGCATCTTCGATCTGGTCCAGTCGATGAAATCGTATTCGCACGTGGATCGC
GGCGCGCAGCAGATGGTGGACATCCACGAGGGCCTCGAAGATACCCTGCGGCTGCTGTCCTTTCGGATCAATTCGGGCGT
AGAAGTACGGCGGTATTACCACCACAATTTGTCCCAAATCCTCGCCTACGGCAGCGAACTCAATCAAGTCTGGACCAACC
TAATCGACAATGCCTTAGACGCGCTGGAAGACCGGGGGATCATCGAGCTGACCACCGGCTGCAAAGGCGAGCATCTGTAC
GTTCAAATCGCCGACAACGGACCGGGCATTCCTGCGGATATCCAGTCGCGGATTTTCGAACCTTTCTATACCACCAAGCC
CGTAGGTAAGGGCTCAGGTCTGGGACTCGATGTCGCCCGGCGCATTATCGAAAATCGCCACCAGGGCACGATCGCCCTCG
AATCGCAACCGGGCCGGACTGTGTTCACCATCCGGTTGCCGCTCGCACTCAATCACAATGGTGGATGA

Upstream 100 bases:

>100_bases
GCTGCGAAACGTTTTTGCGCCGATCCGGCCGCTACGCTGCCGGCGCAGAACCGGGGGCCGCCCTCTCGGTCCCGGCTATA
CTCTATGAGTCGGTTTGCCC

Downstream 100 bases:

>100_bases
TGGGCAGGCTATCTATCTCGGCGACTGGAGAAGCTACTTTTGGCGGCTGTGTGTCTGTTGCGCAACGAGCATCCATGTAG
ATAGGCAGCTCCGGAGGCGA

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MGSFAAMLRVDELLRLEVFAKLPPSRLEWVCGRVRPVVLCAGETLVKEGDPPRGFFILAEGKLGIWRQSDGVEMPIGRHE
APGFVGEVPVLTDEPMLVSVRALTDCRVYELPASDFLELIHTCRDVERLIFREVHHRLRGLESFVRTREKMAALGTLSAG
LAHELNNPAAALVRVLNNVAPAIVELQRMNLIYGQSNPDPKHSEQWLQVRDVGYEAIAHPTEDASVRDDLEEQLLAWLED
YGVEDAWKVAGPLALGRVTPETLAVLIEPWRDHPGELRDMGIRWLALSFDVMEMIQSGLRGSRRIFDLVQSMKSYSHVDR
GAQQMVDIHEGLEDTLRLLSFRINSGVEVRRYYHHNLSQILAYGSELNQVWTNLIDNALDALEDRGIIELTTGCKGEHLY
VQIADNGPGIPADIQSRIFEPFYTTKPVGKGSGLGLDVARRIIENRHQGTIALESQPGRTVFTIRLPLALNHNGG

Sequences:

>Translated_475_residues
MGSFAAMLRVDELLRLEVFAKLPPSRLEWVCGRVRPVVLCAGETLVKEGDPPRGFFILAEGKLGIWRQSDGVEMPIGRHE
APGFVGEVPVLTDEPMLVSVRALTDCRVYELPASDFLELIHTCRDVERLIFREVHHRLRGLESFVRTREKMAALGTLSAG
LAHELNNPAAALVRVLNNVAPAIVELQRMNLIYGQSNPDPKHSEQWLQVRDVGYEAIAHPTEDASVRDDLEEQLLAWLED
YGVEDAWKVAGPLALGRVTPETLAVLIEPWRDHPGELRDMGIRWLALSFDVMEMIQSGLRGSRRIFDLVQSMKSYSHVDR
GAQQMVDIHEGLEDTLRLLSFRINSGVEVRRYYHHNLSQILAYGSELNQVWTNLIDNALDALEDRGIIELTTGCKGEHLY
VQIADNGPGIPADIQSRIFEPFYTTKPVGKGSGLGLDVARRIIENRHQGTIALESQPGRTVFTIRLPLALNHNGG
>Mature_474_residues
GSFAAMLRVDELLRLEVFAKLPPSRLEWVCGRVRPVVLCAGETLVKEGDPPRGFFILAEGKLGIWRQSDGVEMPIGRHEA
PGFVGEVPVLTDEPMLVSVRALTDCRVYELPASDFLELIHTCRDVERLIFREVHHRLRGLESFVRTREKMAALGTLSAGL
AHELNNPAAALVRVLNNVAPAIVELQRMNLIYGQSNPDPKHSEQWLQVRDVGYEAIAHPTEDASVRDDLEEQLLAWLEDY
GVEDAWKVAGPLALGRVTPETLAVLIEPWRDHPGELRDMGIRWLALSFDVMEMIQSGLRGSRRIFDLVQSMKSYSHVDRG
AQQMVDIHEGLEDTLRLLSFRINSGVEVRRYYHHNLSQILAYGSELNQVWTNLIDNALDALEDRGIIELTTGCKGEHLYV
QIADNGPGIPADIQSRIFEPFYTTKPVGKGSGLGLDVARRIIENRHQGTIALESQPGRTVFTIRLPLALNHNGG

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=180, Percent_Identity=34.4444444444444, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788549, Length=170, Percent_Identity=35.8823529411765, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790300, Length=159, Percent_Identity=32.0754716981132, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI145693157, Length=107, Percent_Identity=36.4485981308411, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53156; Mature: 53024

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50042 CNMP_BINDING_3 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSFAAMLRVDELLRLEVFAKLPPSRLEWVCGRVRPVVLCAGETLVKEGDPPRGFFILAE
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCEEEEEC
GKLGIWRQSDGVEMPIGRHEAPGFVGEVPVLTDEPMLVSVRALTDCRVYELPASDFLELI
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCHHHHHHHH
HTCRDVERLIFREVHHRLRGLESFVRTREKMAALGTLSAGLAHELNNPAAALVRVLNNVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
PAIVELQRMNLIYGQSNPDPKHSEQWLQVRDVGYEAIAHPTEDASVRDDLEEQLLAWLED
HHHHHHHHHHEEECCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHH
YGVEDAWKVAGPLALGRVTPETLAVLIEPWRDHPGELRDMGIRWLALSFDVMEMIQSGLR
CCCCHHHHHHCCHHHCCCCHHHHHHHHHHHCCCCCHHHHCCHHHEEHHHHHHHHHHHCCC
GSRRIFDLVQSMKSYSHVDRGAQQMVDIHEGLEDTLRLLSFRINSGVEVRRYYHHNLSQI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LAYGSELNQVWTNLIDNALDALEDRGIIELTTGCKGEHLYVQIADNGPGIPADIQSRIFE
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHCC
PFYTTKPVGKGSGLGLDVARRIIENRHQGTIALESQPGRTVFTIRLPLALNHNGG
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEEEEECCCCC
>Mature Secondary Structure 
GSFAAMLRVDELLRLEVFAKLPPSRLEWVCGRVRPVVLCAGETLVKEGDPPRGFFILAE
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCEEEEEC
GKLGIWRQSDGVEMPIGRHEAPGFVGEVPVLTDEPMLVSVRALTDCRVYELPASDFLELI
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCHHHHHHHH
HTCRDVERLIFREVHHRLRGLESFVRTREKMAALGTLSAGLAHELNNPAAALVRVLNNVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
PAIVELQRMNLIYGQSNPDPKHSEQWLQVRDVGYEAIAHPTEDASVRDDLEEQLLAWLED
HHHHHHHHHHEEECCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHH
YGVEDAWKVAGPLALGRVTPETLAVLIEPWRDHPGELRDMGIRWLALSFDVMEMIQSGLR
CCCCHHHHHHCCHHHCCCCHHHHHHHHHHHCCCCCHHHHCCHHHEEHHHHHHHHHHHCCC
GSRRIFDLVQSMKSYSHVDRGAQQMVDIHEGLEDTLRLLSFRINSGVEVRRYYHHNLSQI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LAYGSELNQVWTNLIDNALDALEDRGIIELTTGCKGEHLYVQIADNGPGIPADIQSRIFE
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHCC
PFYTTKPVGKGSGLGLDVARRIIENRHQGTIALESQPGRTVFTIRLPLALNHNGG
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2710108; 8355615 [H]