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The map label for this gene is maeB [C]

Identifier: 37521718

GI number: 37521718

Start: 2300794

End: 2302182

Strand: Reverse

Name: maeB [C]

Synonym: gll2149

Alternate gene names: 37521718

Gene position: 2302182-2300794 (Counterclockwise)

Preceding gene: 37521720

Following gene: 37521717

Centisome position: 49.41

GC content: 65.87

Gene sequence:

>1389_bases
ATGACTCGGATCACGCCCAATTCCAGCTTCAGCCTGACCCTGCGCCTGCACCTGCCCAACCAGCCGGGGATGCTCGCCAA
GGCAACCGCCGCCATCGGCGAGGCGGGCGGCAACATCGGCGACATCGAGCTGATTGAGCAGGAGCGCGCCTACACCGTGC
GCGATCTCATCGTCGATGCCACCAGCGAGGAGCATGCCCACGCAATCGCCGAGCGGGTAAGCGCCATTCCCGAATTGAAG
CTGCTGCAGGTGGACGACCGGACGTTTCAGATCCACCAGGGCGGCAAGATCCAGATCGTCAGCAAAATTCCGCTCAGGAC
GCGCTCGGATCTGGCGATGGCCTACACCCCCGGCGTCGGGCGCATTTGCATGGCGATTGCCCGCAATCCGGAGCGGGTCT
ATGAACTGACCATCAAGCGCAACACCGTGGCGGTCCTCACCGACGGCTCAGCGGTACTGGGGCTGGGCGACATCGGCCCG
GCCGGGGCGTTGCCGGTCATGGAGGGCAAGGCGCTGTTGTTCAAAGAATTCGCCAATATCGACGCCTTTCCGGTCTGCCT
GAGCACCCAGAATGTCGATGAGATTGTCGAGACCGCCGTGCGCATAAGCACTGTCTTCGGCGGCATCAACCTCGAAGACA
TCAGCGCCCCGCGCTGCTTCGAAGTCGAGCGCCTGCTGCAGGAGCGCCTCGACATTCCGGTCTTTCACGACGATCAGCAC
GGTACCGCCATCGTCGTGCTCGCCGCCCTCGAAAACGCCCTCAAGCTGGTGGACAAGTCCCTCGCATCGGTGCGGATCGT
CATCAATGGGGCGGGGGCGGCGGGGATCGCCATTCACCAGTTGCTCACCGCCGCCGGGGCGCGGGCGATCGTGATGTGCG
ATTCCCACGGCATCGTGAGCGCCGGGCGCGCCGACTTGAACGCCGAGAAGCGTGCGGTCGCCGTCGAGCAGACCGGCGCC
CTGGCCGATGCCATGGCGGGGGCGGATGTGTTTTTGGGGGTGAGCAAAGGGGGGGTGCTCAGCCGCGCGATGGTGCAGAG
CATGGCGACCGACCCGGTGGTCTTCGCCATGGCCAACCCGGTGCCCGAAATCTTGCCTGAAGAAGCCGAAGGGGTGGTGG
CGGTGATGGCCACCGGCCGCAGCGACTATCCCAACCAGATCAACAACGTGCTCGCTTTTCCGGGGATCTTCCGGGGTGCC
CTCGACTGCCGCGCGCGCCGGGTGAATCTGGAGATGAAGATGGCCGCCGCGCGGGCGATCGCCGCCCTGGTGAGCCCCGA
CGAAATTGCGCCCGAATCGATTATCCCGAGCGTGTTCGACCGGCGCGTGGTTGTGGCGGTTTCGGCCGCCGTGCAGCAGG
CTGCCCGCGCCACGGGCGTCGCCCGCTAA

Upstream 100 bases:

>100_bases
AGCTCGGGGAGATCTTTCAATTGCTTGACGGCTATTGTCGGATGGAGCAGGGGTGGAATTCGGCACAGGCTATACTTGGA
CGTCTCGTTTTGCGCCGCCC

Downstream 100 bases:

>100_bases
AGTCGCTTTGCAAGAGGACAAGTCCGGGCTAATCTTGTGCACGCATTGCCAGGAGAGCCCAGATGTTCAAAATCCTTCCC
CCCGCCCTCGCTTGCGCAGC

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTRITPNSSFSLTLRLHLPNQPGMLAKATAAIGEAGGNIGDIELIEQERAYTVRDLIVDATSEEHAHAIAERVSAIPELK
LLQVDDRTFQIHQGGKIQIVSKIPLRTRSDLAMAYTPGVGRICMAIARNPERVYELTIKRNTVAVLTDGSAVLGLGDIGP
AGALPVMEGKALLFKEFANIDAFPVCLSTQNVDEIVETAVRISTVFGGINLEDISAPRCFEVERLLQERLDIPVFHDDQH
GTAIVVLAALENALKLVDKSLASVRIVINGAGAAGIAIHQLLTAAGARAIVMCDSHGIVSAGRADLNAEKRAVAVEQTGA
LADAMAGADVFLGVSKGGVLSRAMVQSMATDPVVFAMANPVPEILPEEAEGVVAVMATGRSDYPNQINNVLAFPGIFRGA
LDCRARRVNLEMKMAAARAIAALVSPDEIAPESIIPSVFDRRVVVAVSAAVQQAARATGVAR

Sequences:

>Translated_462_residues
MTRITPNSSFSLTLRLHLPNQPGMLAKATAAIGEAGGNIGDIELIEQERAYTVRDLIVDATSEEHAHAIAERVSAIPELK
LLQVDDRTFQIHQGGKIQIVSKIPLRTRSDLAMAYTPGVGRICMAIARNPERVYELTIKRNTVAVLTDGSAVLGLGDIGP
AGALPVMEGKALLFKEFANIDAFPVCLSTQNVDEIVETAVRISTVFGGINLEDISAPRCFEVERLLQERLDIPVFHDDQH
GTAIVVLAALENALKLVDKSLASVRIVINGAGAAGIAIHQLLTAAGARAIVMCDSHGIVSAGRADLNAEKRAVAVEQTGA
LADAMAGADVFLGVSKGGVLSRAMVQSMATDPVVFAMANPVPEILPEEAEGVVAVMATGRSDYPNQINNVLAFPGIFRGA
LDCRARRVNLEMKMAAARAIAALVSPDEIAPESIIPSVFDRRVVVAVSAAVQQAARATGVAR
>Mature_461_residues
TRITPNSSFSLTLRLHLPNQPGMLAKATAAIGEAGGNIGDIELIEQERAYTVRDLIVDATSEEHAHAIAERVSAIPELKL
LQVDDRTFQIHQGGKIQIVSKIPLRTRSDLAMAYTPGVGRICMAIARNPERVYELTIKRNTVAVLTDGSAVLGLGDIGPA
GALPVMEGKALLFKEFANIDAFPVCLSTQNVDEIVETAVRISTVFGGINLEDISAPRCFEVERLLQERLDIPVFHDDQHG
TAIVVLAALENALKLVDKSLASVRIVINGAGAAGIAIHQLLTAAGARAIVMCDSHGIVSAGRADLNAEKRAVAVEQTGAL
ADAMAGADVFLGVSKGGVLSRAMVQSMATDPVVFAMANPVPEILPEEAEGVVAVMATGRSDYPNQINNVLAFPGIFRGAL
DCRARRVNLEMKMAAARAIAALVSPDEIAPESIIPSVFDRRVVVAVSAAVQQAARATGVAR

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=414, Percent_Identity=25.8454106280193, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI62420882, Length=411, Percent_Identity=26.2773722627737, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI62420880, Length=411, Percent_Identity=26.2773722627737, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI239049447, Length=411, Percent_Identity=26.2773722627737, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI4505145, Length=440, Percent_Identity=26.1363636363636, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI270265879, Length=373, Percent_Identity=27.6139410187668, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788806, Length=405, Percent_Identity=48.3950617283951, Blast_Score=356, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17537199, Length=487, Percent_Identity=24.6406570841889, Blast_Score=85, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6322823, Length=350, Percent_Identity=25.1428571428571, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24646386, Length=428, Percent_Identity=26.8691588785047, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24646388, Length=428, Percent_Identity=26.8691588785047, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281362674, Length=435, Percent_Identity=25.7471264367816, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281362672, Length=435, Percent_Identity=25.7471264367816, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356279, Length=435, Percent_Identity=25.7471264367816, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281363503, Length=426, Percent_Identity=25.8215962441315, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281363505, Length=426, Percent_Identity=25.8215962441315, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI78707236, Length=426, Percent_Identity=25.8215962441315, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI78707232, Length=426, Percent_Identity=25.8215962441315, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI78707240, Length=460, Percent_Identity=24.3478260869565, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI78707242, Length=460, Percent_Identity=24.3478260869565, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI78707238, Length=460, Percent_Identity=24.3478260869565, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 48819; Mature: 48688

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRITPNSSFSLTLRLHLPNQPGMLAKATAAIGEAGGNIGDIELIEQERAYTVRDLIVDA
CCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEECHHHHHEEEHHHEEC
TSEEHAHAIAERVSAIPELKLLQVDDRTFQIHQGGKIQIVSKIPLRTRSDLAMAYTPGVG
CCHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCEEEEEEECCCCCCCCEEEEECCCHH
RICMAIARNPERVYELTIKRNTVAVLTDGSAVLGLGDIGPAGALPVMEGKALLFKEFANI
HHHHHHHCCCCCEEEEEEECCEEEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHCCC
DAFPVCLSTQNVDEIVETAVRISTVFGGINLEDISAPRCFEVERLLQERLDIPVFHDDQH
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHCCCCEEECCCC
GTAIVVLAALENALKLVDKSLASVRIVINGAGAAGIAIHQLLTAAGARAIVMCDSHGIVS
CCEEEEHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCC
AGRADLNAEKRAVAVEQTGALADAMAGADVFLGVSKGGVLSRAMVQSMATDPVVFAMANP
CCCCCCCCCHHEEEEHHCCCHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCC
VPEILPEEAEGVVAVMATGRSDYPNQINNVLAFPGIFRGALDCRARRVNLEMKMAAARAI
CHHHCCCCCCCEEEEEECCCCCCCHHHHHHEECCHHHHHHHHHHHHEECCHHHHHHHHHH
AALVSPDEIAPESIIPSVFDRRVVVAVSAAVQQAARATGVAR
HHHCCCCCCCCHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TRITPNSSFSLTLRLHLPNQPGMLAKATAAIGEAGGNIGDIELIEQERAYTVRDLIVDA
CCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEECHHHHHEEEHHHEEC
TSEEHAHAIAERVSAIPELKLLQVDDRTFQIHQGGKIQIVSKIPLRTRSDLAMAYTPGVG
CCHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCEEEEEEECCCCCCCCEEEEECCCHH
RICMAIARNPERVYELTIKRNTVAVLTDGSAVLGLGDIGPAGALPVMEGKALLFKEFANI
HHHHHHHCCCCCEEEEEEECCEEEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHCCC
DAFPVCLSTQNVDEIVETAVRISTVFGGINLEDISAPRCFEVERLLQERLDIPVFHDDQH
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHCCCCEEECCCC
GTAIVVLAALENALKLVDKSLASVRIVINGAGAAGIAIHQLLTAAGARAIVMCDSHGIVS
CCEEEEHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCC
AGRADLNAEKRAVAVEQTGALADAMAGADVFLGVSKGGVLSRAMVQSMATDPVVFAMANP
CCCCCCCCCHHEEEEHHCCCHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCC
VPEILPEEAEGVVAVMATGRSDYPNQINNVLAFPGIFRGALDCRARRVNLEMKMAAARAI
CHHHCCCCCCCEEEEEECCCCCCCHHHHHHEECCHHHHHHHHHHHHEECCHHHHHHHHHH
AALVSPDEIAPESIIPSVFDRRVVVAVSAAVQQAARATGVAR
HHHCCCCCCCCHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]