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The map label for this gene is thiO/thiG [H]

Identifier: 37521706

GI number: 37521706

Start: 2288011

End: 2289066

Strand: Reverse

Name: thiO/thiG [H]

Synonym: gll2137

Alternate gene names: 37521706

Gene position: 2289066-2288011 (Counterclockwise)

Preceding gene: 37521712

Following gene: 37521704

Centisome position: 49.13

GC content: 69.41

Gene sequence:

>1056_bases
GTGTTGTCAGGCTGTGGCATGAAAATCTGTATCCTGGGCGGCGGATTAATCGGCCTTGCGGTGGCGCTCGAACTGCGGCT
TGCCGGGGTCGAAGTCGTCGTGCTCGACCGCGGCAGCGTGCCCGCGGGTTGGGCGGCGGCGGGGATGATCGCCCCAGCGG
CCGAGGGATTGGCGGGCGGCCCACTCGCGGATCTGGCTTTTGCAAGCCGCGCGCTGTGGCCCGCCTGGGCGGTGCGCCTC
GAAGAATTCAGCGGCCAGGGAATCGATTACACCCCCTGCGGCGCCCTGGTGCCCGCGGTCGGGGACGCCATTGCCCCAGC
CGAAGGTCAGTGGTGGGACAGCGCCCGGATGGCGAGCGCAGTGCCGGGTCTTGCCCCCGAGATCGCGGGTGGGTGGTGGC
TTGAAAAAGAAGCCTGCGTCGACAACCGCAAGGTGCTCACCGCCCTGCGCCTGGCCGTCGAGCGACTGGGAGCCGAAATT
TGGGCGGGAGTGACGGTCACCGGACCCGCCGATCCGGGTCTTGCCGCCGTGGCCACCAGTGCCGGTCCGGTGGCGGCGGA
CGCCTTCGTGCTCGCCCAGGGGGCCTGGTCAGGGGCCTGGGGACTGCCCGTCCAGCCGCTCAAAGGCCAGATGCTCGCCC
TCGAAAGCGAACCGGGTTGGCTGGGAGCGATCGTCTTTGGCGAGGACATTTATCTGGTGCCGCGCCGGGACGGCCGCATC
GTCCTGGGCGCCACCCAGGAGACAATCGGTTTCACCCCGGGCAACACCGCCGGCGGGATGGCCAGTCTGCTTGCCGCTGC
CCTCACCCTGGTGCCGGGGCTCGCTTCCCACTGCATCCTGGAGCAGTGGTGGGGCTTTCGCCCGGTTTCTCCTGACGCCG
CGCCGCTCATAGGACCGGGTCCGTGGCCGAATCTGTACCTTGCGACCGGTCACCATCGCAACGGGGTTCTGCTCGCACCG
GTCACCGCCCAGCTGGTGACCCGCGCGCTCACCGGCGACGAAGATCCTTTGCTCTCGGCCTTCAGCTACCGGCGCTTCGG
GGGCGATCCGCGCTGA

Upstream 100 bases:

>100_bases
CTCAGCATTTGCGAAGTTTCCATGGCGTTCCTCACCCTTTTGGACGCTCGGACGGTGACGCTGCACCTTTACCTGGAACA
GAATAACAGGGTACTCCCCT

Downstream 100 bases:

>100_bases
AGCGGCCCACCAGGCAGGCCCAGTCGCCTTCACTGAGGGTCTCGGAAAGTGCAAAACCTTGTCCGGCAAGGGCTTCGACC
ACGCGGGGAGCCTGGGTAAG

Product: oxidoreductase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MLSGCGMKICILGGGLIGLAVALELRLAGVEVVVLDRGSVPAGWAAAGMIAPAAEGLAGGPLADLAFASRALWPAWAVRL
EEFSGQGIDYTPCGALVPAVGDAIAPAEGQWWDSARMASAVPGLAPEIAGGWWLEKEACVDNRKVLTALRLAVERLGAEI
WAGVTVTGPADPGLAAVATSAGPVAADAFVLAQGAWSGAWGLPVQPLKGQMLALESEPGWLGAIVFGEDIYLVPRRDGRI
VLGATQETIGFTPGNTAGGMASLLAAALTLVPGLASHCILEQWWGFRPVSPDAAPLIGPGPWPNLYLATGHHRNGVLLAP
VTAQLVTRALTGDEDPLLSAFSYRRFGGDPR

Sequences:

>Translated_351_residues
MLSGCGMKICILGGGLIGLAVALELRLAGVEVVVLDRGSVPAGWAAAGMIAPAAEGLAGGPLADLAFASRALWPAWAVRL
EEFSGQGIDYTPCGALVPAVGDAIAPAEGQWWDSARMASAVPGLAPEIAGGWWLEKEACVDNRKVLTALRLAVERLGAEI
WAGVTVTGPADPGLAAVATSAGPVAADAFVLAQGAWSGAWGLPVQPLKGQMLALESEPGWLGAIVFGEDIYLVPRRDGRI
VLGATQETIGFTPGNTAGGMASLLAAALTLVPGLASHCILEQWWGFRPVSPDAAPLIGPGPWPNLYLATGHHRNGVLLAP
VTAQLVTRALTGDEDPLLSAFSYRRFGGDPR
>Mature_351_residues
MLSGCGMKICILGGGLIGLAVALELRLAGVEVVVLDRGSVPAGWAAAGMIAPAAEGLAGGPLADLAFASRALWPAWAVRL
EEFSGQGIDYTPCGALVPAVGDAIAPAEGQWWDSARMASAVPGLAPEIAGGWWLEKEACVDNRKVLTALRLAVERLGAEI
WAGVTVTGPADPGLAAVATSAGPVAADAFVLAQGAWSGAWGLPVQPLKGQMLALESEPGWLGAIVFGEDIYLVPRRDGRI
VLGATQETIGFTPGNTAGGMASLLAAALTLVPGLASHCILEQWWGFRPVSPDAAPLIGPGPWPNLYLATGHHRNGVLLAP
VTAQLVTRALTGDEDPLLSAFSYRRFGGDPR

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=404, Percent_Identity=26.4851485148515, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 36054; Mature: 36054

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSGCGMKICILGGGLIGLAVALELRLAGVEVVVLDRGSVPAGWAAAGMIAPAAEGLAGG
CCCCCCEEEEEECCCHHHHHHHHHHEECCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCC
PLADLAFASRALWPAWAVRLEEFSGQGIDYTPCGALVPAVGDAIAPAEGQWWDSARMASA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHH
VPGLAPEIAGGWWLEKEACVDNRKVLTALRLAVERLGAEIWAGVTVTGPADPGLAAVATS
CCCCCCHHCCCEEECHHHHCCHHHHHHHHHHHHHHHCHHHHCCEEEECCCCCCCEEEECC
AGPVAADAFVLAQGAWSGAWGLPVQPLKGQMLALESEPGWLGAIVFGEDIYLVPRRDGRI
CCCCHHCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCEEEEEECCCCEE
VLGATQETIGFTPGNTAGGMASLLAAALTLVPGLASHCILEQWWGFRPVSPDAAPLIGPG
EEECCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PWPNLYLATGHHRNGVLLAPVTAQLVTRALTGDEDPLLSAFSYRRFGGDPR
CCCCEEEEECCCCCCEEECCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLSGCGMKICILGGGLIGLAVALELRLAGVEVVVLDRGSVPAGWAAAGMIAPAAEGLAGG
CCCCCCEEEEEECCCHHHHHHHHHHEECCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCC
PLADLAFASRALWPAWAVRLEEFSGQGIDYTPCGALVPAVGDAIAPAEGQWWDSARMASA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHH
VPGLAPEIAGGWWLEKEACVDNRKVLTALRLAVERLGAEIWAGVTVTGPADPGLAAVATS
CCCCCCHHCCCEEECHHHHCCHHHHHHHHHHHHHHHCHHHHCCEEEECCCCCCCEEEECC
AGPVAADAFVLAQGAWSGAWGLPVQPLKGQMLALESEPGWLGAIVFGEDIYLVPRRDGRI
CCCCHHCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCEEEEEECCCCEE
VLGATQETIGFTPGNTAGGMASLLAAALTLVPGLASHCILEQWWGFRPVSPDAAPLIGPG
EEECCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PWPNLYLATGHHRNGVLLAPVTAQLVTRALTGDEDPLLSAFSYRRFGGDPR
CCCCEEEEECCCCCCEEECCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 12 {D-alpha-aminobutyrate}} 6 {D-phenylalanine}} 14 {D-glutamine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 19 {D-serine}} 0.15 {cytochrome} 29 {D-alanine}} 6.4

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]