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The map label for this gene is rlmN

Identifier: 37521648

GI number: 37521648

Start: 2230579

End: 2231625

Strand: Reverse

Name: rlmN

Synonym: gll2079

Alternate gene names: 37521648

Gene position: 2231625-2230579 (Counterclockwise)

Preceding gene: 37521650

Following gene: 37521638

Centisome position: 47.9

GC content: 65.43

Gene sequence:

>1047_bases
ATGACTACACCTTTGCTTGGCCAGAGCGCCGAGGAACTGCGCATCTGGGTTGAATCCCAGGGCCAGCCTGCCTACCGCGC
CCAACAGTTGCACCGTTGGCTTTACCAGCGGGGGGTGCGCTCGCTGATGGAGATTACCGACTGGCCCAAGGCGTGGCGCG
AACAGGTGCACTCGGTGCCGGTGGGCCGCTCGCAGGTCGTGCGCCAAAGCGCTGCCGCCGACGGCACGATCAAGTACCTG
CTTGCGGGGGCCGACGGCGAAACCGTCGAGACCGTGGGTATTCCGGCCGCCGAGCGGCTGACGGTCTGCGTTTCGTCGCA
GGTGGGTTGTCCCATGGCCTGCCGCTTCTGCGCCACCGGCCAATCCGGTTTTGCCCGCAACCTGGGTGTGCACGAGATCG
TCGATCAGGTGCTCACGGTGCAGGAGGGTTTCGGCCGCCGGGTGAGCCACGTTGTCTTCATGGGCATGGGTGAGCCGCTG
TTGAACCTGGGAGCGGTGGTGCAGGCCCTCCGGGTGCTCAATGGCGACATCGGCATCGGCCAGCGGCAGATCACCGTCTC
GACGGTGGGAGTACCGGGCCAGATTCGGCGGCTTGGAACCTACAAGCTGCAGATCACCCTGGCGGTGAGCCTGCACGCCC
CCAATCAGGATCTGCGTCTCAAACTCATCCCAACCGCCCAGCACTATCCGATCGAGGAGCTGCTCGAAGACTGCCGCGAC
TATGTCGAGACGACCAACCGCCGGGTGAGCTTCGAGTACACGCTACTAGCCGGTATCAACGACGAGCCGCACCACGCCCG
CGAACTGGCGGCGATTTTGCGCGGCTTCCAATCCCACGTCAATTTGATTCCTTACAACCCGATCGAAGGGGTCGAGTACG
AGCGGCCGGGCGAGGCACGGGTGCGCGCTTTTGAGCGCGAACTTGTCCGCCACAAGATTGCCGCGAGCGTGCGGCACACC
CGCGGTCTAGAGGAAGCGGCGGCCTGCGGCCAACTCAGGCGGCGCAGCCTGAGCGGCGAGGCGCAATCGGCGAGCACTCG
CGCCTGA

Upstream 100 bases:

>100_bases
CTTCAAAACGCTTCAAAGGATACTACCTGCCGGGAGATGGCGCAATACGGGCGGGAGCTTCGCTTGCGCACTATGATCGA
AGGAGTGTTTTTGATCGGTC

Downstream 100 bases:

>100_bases
ATCACCCATTGATACTTTTTAGGGGGTGACGGTGAACTTGGTGGGGCTGGAGGTGCGGCCGTTAGGCACCAAGACGACGA
TCTTGCCGGTCTTGGAACCG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MTTPLLGQSAEELRIWVESQGQPAYRAQQLHRWLYQRGVRSLMEITDWPKAWREQVHSVPVGRSQVVRQSAAADGTIKYL
LAGADGETVETVGIPAAERLTVCVSSQVGCPMACRFCATGQSGFARNLGVHEIVDQVLTVQEGFGRRVSHVVFMGMGEPL
LNLGAVVQALRVLNGDIGIGQRQITVSTVGVPGQIRRLGTYKLQITLAVSLHAPNQDLRLKLIPTAQHYPIEELLEDCRD
YVETTNRRVSFEYTLLAGINDEPHHARELAAILRGFQSHVNLIPYNPIEGVEYERPGEARVRAFERELVRHKIAASVRHT
RGLEEAAACGQLRRRSLSGEAQSASTRA

Sequences:

>Translated_348_residues
MTTPLLGQSAEELRIWVESQGQPAYRAQQLHRWLYQRGVRSLMEITDWPKAWREQVHSVPVGRSQVVRQSAAADGTIKYL
LAGADGETVETVGIPAAERLTVCVSSQVGCPMACRFCATGQSGFARNLGVHEIVDQVLTVQEGFGRRVSHVVFMGMGEPL
LNLGAVVQALRVLNGDIGIGQRQITVSTVGVPGQIRRLGTYKLQITLAVSLHAPNQDLRLKLIPTAQHYPIEELLEDCRD
YVETTNRRVSFEYTLLAGINDEPHHARELAAILRGFQSHVNLIPYNPIEGVEYERPGEARVRAFERELVRHKIAASVRHT
RGLEEAAACGQLRRRSLSGEAQSASTRA
>Mature_347_residues
TTPLLGQSAEELRIWVESQGQPAYRAQQLHRWLYQRGVRSLMEITDWPKAWREQVHSVPVGRSQVVRQSAAADGTIKYLL
AGADGETVETVGIPAAERLTVCVSSQVGCPMACRFCATGQSGFARNLGVHEIVDQVLTVQEGFGRRVSHVVFMGMGEPLL
NLGAVVQALRVLNGDIGIGQRQITVSTVGVPGQIRRLGTYKLQITLAVSLHAPNQDLRLKLIPTAQHYPIEELLEDCRDY
VETTNRRVSFEYTLLAGINDEPHHARELAAILRGFQSHVNLIPYNPIEGVEYERPGEARVRAFERELVRHKIAASVRHTR
GLEEAAACGQLRRRSLSGEAQSASTRA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=341, Percent_Identity=40.1759530791789, Blast_Score=253, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_GLOVI (Q7NIV3)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925025.1
- ProteinModelPortal:   Q7NIV3
- GeneID:   2600481
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll2079
- NMPDR:   fig|251221.1.peg.2079
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- BioCyc:   GVIO251221:GLL2079-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 38511; Mature: 38379

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPLLGQSAEELRIWVESQGQPAYRAQQLHRWLYQRGVRSLMEITDWPKAWREQVHSVP
CCCCCCCCCHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
VGRSQVVRQSAAADGTIKYLLAGADGETVETVGIPAAERLTVCVSSQVGCPMACRFCATG
CCHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCHHHEEEEECCCCCCCHHHHHHCCC
QSGFARNLGVHEIVDQVLTVQEGFGRRVSHVVFMGMGEPLLNLGAVVQALRVLNGDIGIG
CCCCHHCCCHHHHHHHHHHHHHHCCHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCC
QRQITVSTVGVPGQIRRLGTYKLQITLAVSLHAPNQDLRLKLIPTAQHYPIEELLEDCRD
CEEEEEEEECCCHHHHHCEEEEEEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHH
YVETTNRRVSFEYTLLAGINDEPHHARELAAILRGFQSHVNLIPYNPIEGVEYERPGEAR
HHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
VRAFERELVRHKIAASVRHTRGLEEAAACGQLRRRSLSGEAQSASTRA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TTPLLGQSAEELRIWVESQGQPAYRAQQLHRWLYQRGVRSLMEITDWPKAWREQVHSVP
CCCCCCCCHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
VGRSQVVRQSAAADGTIKYLLAGADGETVETVGIPAAERLTVCVSSQVGCPMACRFCATG
CCHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCHHHEEEEECCCCCCCHHHHHHCCC
QSGFARNLGVHEIVDQVLTVQEGFGRRVSHVVFMGMGEPLLNLGAVVQALRVLNGDIGIG
CCCCHHCCCHHHHHHHHHHHHHHCCHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCC
QRQITVSTVGVPGQIRRLGTYKLQITLAVSLHAPNQDLRLKLIPTAQHYPIEELLEDCRD
CEEEEEEEECCCHHHHHCEEEEEEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHH
YVETTNRRVSFEYTLLAGINDEPHHARELAAILRGFQSHVNLIPYNPIEGVEYERPGEAR
HHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
VRAFERELVRHKIAASVRHTRGLEEAAACGQLRRRSLSGEAQSASTRA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292