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The map label for this gene is def [H]

Identifier: 37521298

GI number: 37521298

Start: 1834420

End: 1835247

Strand: Direct

Name: def [H]

Synonym: glr1729

Alternate gene names: 37521298

Gene position: 1834420-1835247 (Clockwise)

Preceding gene: 37521296

Following gene: 37521299

Centisome position: 39.37

GC content: 60.39

Gene sequence:

>828_bases
ATGGGTGATAACCCCTTCGGCAAGCCGCTTCGAAGTACGTTCCTTTACTGCCGAGCCACGCATCATAGACCTTGTAGACC
CGAAATAAAACTTTGCCTCCAGCCTGCAGACTGCGTCGCCTGCGGCGAAAGCGATCATTTCTATTTGCATCTTAAGCGTG
CATACCCAAAAGACTGTCTATCCGGAGTAGGGTCTAACGCCTGCCGAATTGGGGATCGTCAGAGTCGCTATCCGGTGACA
ATAGAGTGCGAATCCACGGGCACCAGCGGAGGGGGCGTGTACGAAATCGTCAAAACCGGAGATCCGGTGCTGCGCCTGAC
GGCCAAGCCCTTGAACAGCGATGAAATTCAAAGCGAAGCGATCCAGCAACTGATCGCCGCGATGGCCGAGCGGATGCGCG
AGGCGCCGGGGGTAGGCCTCGCCGCCCCCCAAGTGGGCGTCTCGGTGCAACTGGTGGTCATCGAGGATCGCCCCGAGTAC
ATCGAGCGGCTGAGCGGTGCGGAGCGGCGCGAACGGGAGCGCGAGCCGGTGCCCTTTCATGTGCTCATCAACCCTGTGCT
GAGCGTGGAAGGCGAAGAATCGGCCGTTTTTTTCGAGGGTTGTTTGAGCATTCCCGGTTACCAGGGGTTGGTGGCCCGGG
CGCGGGTGGTGCGGGTCGAAGCACTCGACGAACGGGCCGCCCCGGTCGTCATCCGGGCACACGGCTGGTACGCCCGCATC
CTCCAGCACGAAATCGATCACCTCAACGGCCTACTGTGCGTGGATCGCATGGATCTTCAGACTTTCAGCACCCTCGAAAA
TTACGACCGCTTCTGGCGGGGAGGGTGA

Upstream 100 bases:

>100_bases
GCGATAACTCGCCAATAGGTAAGCGCAGCGCTGCGGCGAAACTGAAGATCCACAGGCCGATAATCACCAGAGCCCACCAG
AGGCCGGAAGAATCGTTCTT

Downstream 100 bases:

>100_bases
TTGGACAGGTGCCAGGTGTCAGGGTAAAAACCGTTGTCCATCGGGTTTTTTATTTGACCCTTGCCTGAGTTGCCCCACTT
CCCGATACTTCTGTAGCCCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MGDNPFGKPLRSTFLYCRATHHRPCRPEIKLCLQPADCVACGESDHFYLHLKRAYPKDCLSGVGSNACRIGDRQSRYPVT
IECESTGTSGGGVYEIVKTGDPVLRLTAKPLNSDEIQSEAIQQLIAAMAERMREAPGVGLAAPQVGVSVQLVVIEDRPEY
IERLSGAERREREREPVPFHVLINPVLSVEGEESAVFFEGCLSIPGYQGLVARARVVRVEALDERAAPVVIRAHGWYARI
LQHEIDHLNGLLCVDRMDLQTFSTLENYDRFWRGG

Sequences:

>Translated_275_residues
MGDNPFGKPLRSTFLYCRATHHRPCRPEIKLCLQPADCVACGESDHFYLHLKRAYPKDCLSGVGSNACRIGDRQSRYPVT
IECESTGTSGGGVYEIVKTGDPVLRLTAKPLNSDEIQSEAIQQLIAAMAERMREAPGVGLAAPQVGVSVQLVVIEDRPEY
IERLSGAERREREREPVPFHVLINPVLSVEGEESAVFFEGCLSIPGYQGLVARARVVRVEALDERAAPVVIRAHGWYARI
LQHEIDHLNGLLCVDRMDLQTFSTLENYDRFWRGG
>Mature_274_residues
GDNPFGKPLRSTFLYCRATHHRPCRPEIKLCLQPADCVACGESDHFYLHLKRAYPKDCLSGVGSNACRIGDRQSRYPVTI
ECESTGTSGGGVYEIVKTGDPVLRLTAKPLNSDEIQSEAIQQLIAAMAERMREAPGVGLAAPQVGVSVQLVVIEDRPEYI
ERLSGAERREREREPVPFHVLINPVLSVEGEESAVFFEGCLSIPGYQGLVARARVVRVEALDERAAPVVIRAHGWYARIL
QHEIDHLNGLLCVDRMDLQTFSTLENYDRFWRGG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=170, Percent_Identity=37.6470588235294, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=37.1951219512195, Blast_Score=84, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24645728, Length=170, Percent_Identity=38.2352941176471, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24645726, Length=168, Percent_Identity=35.7142857142857, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 30616; Mature: 30485

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDNPFGKPLRSTFLYCRATHHRPCRPEIKLCLQPADCVACGESDHFYLHLKRAYPKDCL
CCCCCCCCHHHHHEEHEECCCCCCCCCCHHEEECCCCEEEECCCCEEEEEEECCCCHHHH
SGVGSNACRIGDRQSRYPVTIECESTGTSGGGVYEIVKTGDPVLRLTAKPLNSDEIQSEA
HHCCCCCEECCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHH
IQQLIAAMAERMREAPGVGLAAPQVGVSVQLVVIEDRPEYIERLSGAERREREREPVPFH
HHHHHHHHHHHHHHCCCCCEECCCCCCEEEEEEECCCHHHHHHHCCHHHHHHCCCCCCEE
VLINPVLSVEGEESAVFFEGCLSIPGYQGLVARARVVRVEALDERAAPVVIRAHGWYARI
EEECCCCCCCCCCCEEEEEHHHCCCCCHHHHHHHHHHEEEHHCCCCCCEEEEECCHHHHH
LQHEIDHLNGLLCVDRMDLQTFSTLENYDRFWRGG
HHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GDNPFGKPLRSTFLYCRATHHRPCRPEIKLCLQPADCVACGESDHFYLHLKRAYPKDCL
CCCCCCCHHHHHEEHEECCCCCCCCCCHHEEECCCCEEEECCCCEEEEEEECCCCHHHH
SGVGSNACRIGDRQSRYPVTIECESTGTSGGGVYEIVKTGDPVLRLTAKPLNSDEIQSEA
HHCCCCCEECCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHH
IQQLIAAMAERMREAPGVGLAAPQVGVSVQLVVIEDRPEYIERLSGAERREREREPVPFH
HHHHHHHHHHHHHHCCCCCEECCCCCCEEEEEEECCCHHHHHHHCCHHHHHHCCCCCCEE
VLINPVLSVEGEESAVFFEGCLSIPGYQGLVARARVVRVEALDERAAPVVIRAHGWYARI
EEECCCCCCCCCCCEEEEEHHHCCCCCHHHHHHHHHHEEEHHCCCCCCEEEEECCHHHHH
LQHEIDHLNGLLCVDRMDLQTFSTLENYDRFWRGG
HHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]