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The map label for this gene is irlS [H]

Identifier: 37521255

GI number: 37521255

Start: 1797837

End: 1799198

Strand: Reverse

Name: irlS [H]

Synonym: gll1686

Alternate gene names: 37521255

Gene position: 1799198-1797837 (Counterclockwise)

Preceding gene: 37521257

Following gene: 37521254

Centisome position: 38.62

GC content: 67.47

Gene sequence:

>1362_bases
GTGCCCGCCGACCGACCGCGCGCCGAGCCGCGCAATCCGATCAAGCAGACCCGCCTGCGGTTGTTGGTGAGCTATATTGC
CGTCACCGCCGCGATCATGGCCCTGTTTGCCGTCGGTTTTTACACCTACGTGCGCAGCACCCTGGTCGAACGGGTCGACG
ATACCCTCGACCACGTCGTGGAGCTTGTCGAGCGCTCGCTGGTGGTGGTGCCCGGACCGGACGGCGAACCGGTCGTCGAT
GCCAACTACGTCTTCGCGGGGGGCGACGACGACCCGACCGTCGAGATGGACTTGATTTATCTGGAGTGGTTCGATAGGCG
CGGCCAGGTGGTGCGCTCGACAGCCGTCTTTCCGCGGCCGGTGCCGCTGGTGCGCGGTTCGCTGCACGCGACGGTCGAAG
TGAGCCCGGATCACAAGCTTCGGCAGGTGACGGTGCCGATGCGCCGTGCGGGCCACCTGCTGGGCTACCTGCGCGTCTCG
CACCAGTGGTTCGAAGTCGACAGACCCAGCCGGCAGCTTTTTATCGAACTGAGCGCGGGCGTCGTGCTCACCATGGTGAT
GATTGGCTTGGGCGGCTGGTTTCTGACGGAACTCGCCCTCAGACCGGTGCGCGAGTCCTACGACCGGCTCAAACAGTTCA
CCGCCGATGCGAGCCACGAGCTGCGCACGCCGCTAGCCGCGCTGCAGACCAACGTGCAGGTGGCCCTCGCCGACCCGCAG
CCGAGTGTGGATGAGTACCGCAGGAGCCTGGAAGTGACCGAGCGGCTCACCCGCCGCCTGGGGCGGCTGGTGGCGGATTT
GTTGTTCCTGGCGCGCAACGAAGGTCTGCAGAGCGCCGAGGGCCGGATCTGCTGCAACCTCACCGAGATCCTCCAACAGG
TCGTCGAGGAGCAGACTCCCCTCGCCCAGGCTGCGGATCTTGCGGTGCAGCTCAAAAGCGGCGGGCCGCTCACCGTCACC
GCCAACCCCGACCAGATGCATCAACTGTTTACCAATTTGCTGAGCAACGCCATTCGCTACACCCCCGCCGGGGGGCGGGT
ATGCCTCGGTACTGCGCGCGACGGCCGCGACGCGCTGGTGGAAGTGAGCGACACCGGCATCGGCATCGCCCCGGAGCACC
TGGAACGGATATTCGAGCGCTTCTGGCGGGTCGATTCGGCCCGCACCCGCCAGGCGGGCGGCAGTGGCCTGGGCCTGGCT
ATCGCGAGGACGATCGTCGAAAACCACCGGGGGGAGATTCACGCCCTGAGCGAACCCGGCCGGGGTACCACCCTGCGGGT
GCGCCTGCCCGGGCGTCCCGACGGGCAGGAACGGCGCCCAGCCTCCGAAAACACCCCCGGCGCGCTTCCCTTGGAGGGCT
GA

Upstream 100 bases:

>100_bases
CTTCGAAACGGTCGGTATCCACTAGGACGGCGGGCCCGGCGCCCGCGTTCAACACCGCAGAGCCAGGTTTAATAGAAAGT
AATCGTCACTCGCTGCACCT

Downstream 100 bases:

>100_bases
GTCGCGCCATACTGTGGACGGGCGTTCCGGCCGCTTTTCGATGAATCTTTTCACCCGCAAATCGCGCACCTACCACGACC
CGATCCACGGGGCGATCAGC

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MPADRPRAEPRNPIKQTRLRLLVSYIAVTAAIMALFAVGFYTYVRSTLVERVDDTLDHVVELVERSLVVVPGPDGEPVVD
ANYVFAGGDDDPTVEMDLIYLEWFDRRGQVVRSTAVFPRPVPLVRGSLHATVEVSPDHKLRQVTVPMRRAGHLLGYLRVS
HQWFEVDRPSRQLFIELSAGVVLTMVMIGLGGWFLTELALRPVRESYDRLKQFTADASHELRTPLAALQTNVQVALADPQ
PSVDEYRRSLEVTERLTRRLGRLVADLLFLARNEGLQSAEGRICCNLTEILQQVVEEQTPLAQAADLAVQLKSGGPLTVT
ANPDQMHQLFTNLLSNAIRYTPAGGRVCLGTARDGRDALVEVSDTGIGIAPEHLERIFERFWRVDSARTRQAGGSGLGLA
IARTIVENHRGEIHALSEPGRGTTLRVRLPGRPDGQERRPASENTPGALPLEG

Sequences:

>Translated_453_residues
MPADRPRAEPRNPIKQTRLRLLVSYIAVTAAIMALFAVGFYTYVRSTLVERVDDTLDHVVELVERSLVVVPGPDGEPVVD
ANYVFAGGDDDPTVEMDLIYLEWFDRRGQVVRSTAVFPRPVPLVRGSLHATVEVSPDHKLRQVTVPMRRAGHLLGYLRVS
HQWFEVDRPSRQLFIELSAGVVLTMVMIGLGGWFLTELALRPVRESYDRLKQFTADASHELRTPLAALQTNVQVALADPQ
PSVDEYRRSLEVTERLTRRLGRLVADLLFLARNEGLQSAEGRICCNLTEILQQVVEEQTPLAQAADLAVQLKSGGPLTVT
ANPDQMHQLFTNLLSNAIRYTPAGGRVCLGTARDGRDALVEVSDTGIGIAPEHLERIFERFWRVDSARTRQAGGSGLGLA
IARTIVENHRGEIHALSEPGRGTTLRVRLPGRPDGQERRPASENTPGALPLEG
>Mature_452_residues
PADRPRAEPRNPIKQTRLRLLVSYIAVTAAIMALFAVGFYTYVRSTLVERVDDTLDHVVELVERSLVVVPGPDGEPVVDA
NYVFAGGDDDPTVEMDLIYLEWFDRRGQVVRSTAVFPRPVPLVRGSLHATVEVSPDHKLRQVTVPMRRAGHLLGYLRVSH
QWFEVDRPSRQLFIELSAGVVLTMVMIGLGGWFLTELALRPVRESYDRLKQFTADASHELRTPLAALQTNVQVALADPQP
SVDEYRRSLEVTERLTRRLGRLVADLLFLARNEGLQSAEGRICCNLTEILQQVVEEQTPLAQAADLAVQLKSGGPLTVTA
NPDQMHQLFTNLLSNAIRYTPAGGRVCLGTARDGRDALVEVSDTGIGIAPEHLERIFERFWRVDSARTRQAGGSGLGLAI
ARTIVENHRGEIHALSEPGRGTTLRVRLPGRPDGQERRPASENTPGALPLEG

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=230, Percent_Identity=35.2173913043478, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786600, Length=224, Percent_Identity=36.1607142857143, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788393, Length=226, Percent_Identity=33.1858407079646, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1790346, Length=223, Percent_Identity=33.1838565022422, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693157, Length=221, Percent_Identity=30.316742081448, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789149, Length=239, Percent_Identity=31.3807531380753, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788279, Length=231, Percent_Identity=28.1385281385281, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082128, Length=227, Percent_Identity=30.3964757709251, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790436, Length=230, Percent_Identity=29.1304347826087, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789403, Length=223, Percent_Identity=34.9775784753363, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI87081816, Length=228, Percent_Identity=32.4561403508772, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994928, Length=244, Percent_Identity=28.6885245901639, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788713, Length=225, Percent_Identity=25.7777777777778, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1790861, Length=216, Percent_Identity=30.5555555555556, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788549, Length=235, Percent_Identity=27.6595744680851, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786912, Length=217, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789808, Length=228, Percent_Identity=27.1929824561404, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790551, Length=232, Percent_Identity=30.1724137931034, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787894, Length=201, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790300, Length=229, Percent_Identity=30.5676855895196, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787374, Length=225, Percent_Identity=28.8888888888889, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50108; Mature: 49977

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPADRPRAEPRNPIKQTRLRLLVSYIAVTAAIMALFAVGFYTYVRSTLVERVDDTLDHVV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELVERSLVVVPGPDGEPVVDANYVFAGGDDDPTVEMDLIYLEWFDRRGQVVRSTAVFPRP
HHHHCCEEEEECCCCCCEEECCEEEECCCCCCCEEEEEEEEEHHCCCCCEEHHHCCCCCC
VPLVRGSLHATVEVSPDHKLRQVTVPMRRAGHLLGYLRVSHQWFEVDRPSRQLFIELSAG
CHHHCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEECHH
VVLTMVMIGLGGWFLTELALRPVRESYDRLKQFTADASHELRTPLAALQTNVQVALADPQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHCCCEEEEECCC
PSVDEYRRSLEVTERLTRRLGRLVADLLFLARNEGLQSAEGRICCNLTEILQQVVEEQTP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHHHCCC
LAQAADLAVQLKSGGPLTVTANPDQMHQLFTNLLSNAIRYTPAGGRVCLGTARDGRDALV
HHHHHHHEEEEECCCCEEEECCHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCEE
EVSDTGIGIAPEHLERIFERFWRVDSARTRQAGGSGLGLAIARTIVENHRGEIHALSEPG
EEECCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCC
RGTTLRVRLPGRPDGQERRPASENTPGALPLEG
CCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PADRPRAEPRNPIKQTRLRLLVSYIAVTAAIMALFAVGFYTYVRSTLVERVDDTLDHVV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELVERSLVVVPGPDGEPVVDANYVFAGGDDDPTVEMDLIYLEWFDRRGQVVRSTAVFPRP
HHHHCCEEEEECCCCCCEEECCEEEECCCCCCCEEEEEEEEEHHCCCCCEEHHHCCCCCC
VPLVRGSLHATVEVSPDHKLRQVTVPMRRAGHLLGYLRVSHQWFEVDRPSRQLFIELSAG
CHHHCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEECHH
VVLTMVMIGLGGWFLTELALRPVRESYDRLKQFTADASHELRTPLAALQTNVQVALADPQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHCCCEEEEECCC
PSVDEYRRSLEVTERLTRRLGRLVADLLFLARNEGLQSAEGRICCNLTEILQQVVEEQTP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHHHCCC
LAQAADLAVQLKSGGPLTVTANPDQMHQLFTNLLSNAIRYTPAGGRVCLGTARDGRDALV
HHHHHHHEEEEECCCCEEEECCHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCEE
EVSDTGIGIAPEHLERIFERFWRVDSARTRQAGGSGLGLAIARTIVENHRGEIHALSEPG
EEECCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCC
RGTTLRVRLPGRPDGQERRPASENTPGALPLEG
CCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]