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The map label for this gene is pmbA [H]

Identifier: 37521131

GI number: 37521131

Start: 1682369

End: 1683676

Strand: Reverse

Name: pmbA [H]

Synonym: gll1562

Alternate gene names: 37521131

Gene position: 1683676-1682369 (Counterclockwise)

Preceding gene: 37521132

Following gene: 37521130

Centisome position: 36.14

GC content: 67.05

Gene sequence:

>1308_bases
ATGGCTTTTCTGAACGCCGAGCGGGCGCAAAAAATGGTCGAAACCGCCCTCAAAGCGGCGAGCGCCAAAGATGTGCTCGT
GCGCCTGGGTGAGCGCCGCAATGCCGTCACCCGCTTTGCCAACAACGCCATCACCCAGAACACCCTGGATGCGGGCACCG
AGTTGGCAGTCGAAGTCGCCTTCGAGAACCGCCGCGCCAGTGCCCGGGTGAGCAGCCTGAGCGAAGAGGCGATCCGCACG
GCGGTGCGGCGGGCCGAGGAGCTTGCCCGGGTGGCCCGGCCCGACCCGGAATATCTGCCGCCTTTGCCCCGGCAGCAGTA
TCTGGCGGTGGATGCCTACGACCCGGCCACCGCCAACCTTACACCGGTCGAGCGCGCCAAACGCGCCGGGCGCATCGCCG
GGGCGGCGGGCCGGGCGAGCCTGCGGGCGGCCGGAACGGTCGAAAGCGCTGAGCGCGTCTCGGTGGTGGCGGCAAGTAGC
GGCCTGTTTGCCTACCACCTGCGCACCGATGCCCAGATTGGCTGCACGCTGCAGGGACCGGACAGTTCCGGCTGGGCGCG
CGATGTCGCCTCGCGCATGGCGCTGCTGTCGCCGGAGCGGATCGCCCAAAGCGCCATCGAGCAGGCCCGCCGGGGCGTCA
ATCCCGTGGCCGTCGAGCCGGGCCGCTACACGGTCATCCTCGCCCCGGCGGCGGTGGGCACGCTGCTCTTTCACCTGGTG
GAGCAGATGGACGCCCGCGACACCCTGGACGGGGTGACGTTCCTGTCGGGCAAAATGGGCCGGCAACTGGTCGGCTCGAA
TATCACTCTGCGCACTGACCCTGCCAATACCCGTCTGCCGGGGGTGCCCTTCGATGAGGAAGGATTGCCCCAACCGCAAT
TGCCTTGGATCGATCGGGGTGTGTTCCAGCAGATGCGCTGGGACCGCTTCACCGCCCAAAAAAATAACCGCCCCCCGGTG
CCCGCCCCCCGGAGCCTGGTGATGGAGGGCGAGGACAGAAGCATCGAAGATCTGATTCGCTCCACCGAGCGGGGGCTGCT
CGTCACCCACGTCTGGTACGTCCGCGACGTCAAACCCGACGAAACAACGGTCACCGGCCTGACGCGCGACGGCACATTTT
TGATCGAAAAGGGTCAACTGACCCGGGGAGTGAAGGTGCTTCGCTTCAACCAGAGCCTGCTGGCCATGCTCTCCAGCACG
CTGGCTTTAAGCCGCCCCGAGGCGGCGGCCGACAACGAACTGCCCCCCAGCCTGCTGCCGGCCCTCAAGGTGGCCGAGTT
CAATTTTGTGAGCGGCAGCAGCTTTTAG

Upstream 100 bases:

>100_bases
GCGGCAAAGGCCAGCCGGGGCAGGCGGGGCGGATGACCCACGGCGCCGCCCCGGCCCGCTTCCGGCAGATTGAAGTCGGT
TCGGGTCGAAAGATTTAGCT

Downstream 100 bases:

>100_bases
CCGGGGCAGAATATAAGCAACCATTTTCGGTCCGCTCCAGGAGATCCGTCCGAATGATCGCCAATCCGAAGCGGCAGGGT
CTGACGCCGCAGGAGTACTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MAFLNAERAQKMVETALKAASAKDVLVRLGERRNAVTRFANNAITQNTLDAGTELAVEVAFENRRASARVSSLSEEAIRT
AVRRAEELARVARPDPEYLPPLPRQQYLAVDAYDPATANLTPVERAKRAGRIAGAAGRASLRAAGTVESAERVSVVAASS
GLFAYHLRTDAQIGCTLQGPDSSGWARDVASRMALLSPERIAQSAIEQARRGVNPVAVEPGRYTVILAPAAVGTLLFHLV
EQMDARDTLDGVTFLSGKMGRQLVGSNITLRTDPANTRLPGVPFDEEGLPQPQLPWIDRGVFQQMRWDRFTAQKNNRPPV
PAPRSLVMEGEDRSIEDLIRSTERGLLVTHVWYVRDVKPDETTVTGLTRDGTFLIEKGQLTRGVKVLRFNQSLLAMLSST
LALSRPEAAADNELPPSLLPALKVAEFNFVSGSSF

Sequences:

>Translated_435_residues
MAFLNAERAQKMVETALKAASAKDVLVRLGERRNAVTRFANNAITQNTLDAGTELAVEVAFENRRASARVSSLSEEAIRT
AVRRAEELARVARPDPEYLPPLPRQQYLAVDAYDPATANLTPVERAKRAGRIAGAAGRASLRAAGTVESAERVSVVAASS
GLFAYHLRTDAQIGCTLQGPDSSGWARDVASRMALLSPERIAQSAIEQARRGVNPVAVEPGRYTVILAPAAVGTLLFHLV
EQMDARDTLDGVTFLSGKMGRQLVGSNITLRTDPANTRLPGVPFDEEGLPQPQLPWIDRGVFQQMRWDRFTAQKNNRPPV
PAPRSLVMEGEDRSIEDLIRSTERGLLVTHVWYVRDVKPDETTVTGLTRDGTFLIEKGQLTRGVKVLRFNQSLLAMLSST
LALSRPEAAADNELPPSLLPALKVAEFNFVSGSSF
>Mature_434_residues
AFLNAERAQKMVETALKAASAKDVLVRLGERRNAVTRFANNAITQNTLDAGTELAVEVAFENRRASARVSSLSEEAIRTA
VRRAEELARVARPDPEYLPPLPRQQYLAVDAYDPATANLTPVERAKRAGRIAGAAGRASLRAAGTVESAERVSVVAASSG
LFAYHLRTDAQIGCTLQGPDSSGWARDVASRMALLSPERIAQSAIEQARRGVNPVAVEPGRYTVILAPAAVGTLLFHLVE
QMDARDTLDGVTFLSGKMGRQLVGSNITLRTDPANTRLPGVPFDEEGLPQPQLPWIDRGVFQQMRWDRFTAQKNNRPPVP
APRSLVMEGEDRSIEDLIRSTERGLLVTHVWYVRDVKPDETTVTGLTRDGTFLIEKGQLTRGVKVLRFNQSLLAMLSSTL
ALSRPEAAADNELPPSLLPALKVAEFNFVSGSSF

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=441, Percent_Identity=23.3560090702948, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47357; Mature: 47226

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFLNAERAQKMVETALKAASAKDVLVRLGERRNAVTRFANNAITQNTLDAGTELAVEVA
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEEEE
FENRRASARVSSLSEEAIRTAVRRAEELARVARPDPEYLPPLPRQQYLAVDAYDPATANL
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCC
TPVERAKRAGRIAGAAGRASLRAAGTVESAERVSVVAASSGLFAYHLRTDAQIGCTLQGP
CHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHEEEEEECCCEEEEEEECCCEEEEEEECC
DSSGWARDVASRMALLSPERIAQSAIEQARRGVNPVAVEPGRYTVILAPAAVGTLLFHLV
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEECCCCEEEEECHHHHHHHHHHHH
EQMDARDTLDGVTFLSGKMGRQLVGSNITLRTDPANTRLPGVPFDEEGLPQPQLPWIDRG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHH
VFQQMRWDRFTAQKNNRPPVPAPRSLVMEGEDRSIEDLIRSTERGLLVTHVWYVRDVKPD
HHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCHHHHHHHHCCCEEEEEEEEEEECCCC
ETTVTGLTRDGTFLIEKGQLTRGVKVLRFNQSLLAMLSSTLALSRPEAAADNELPPSLLP
CCEEEEEECCCEEEEECCCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
ALKVAEFNFVSGSSF
HHHHHEEECCCCCCC
>Mature Secondary Structure 
AFLNAERAQKMVETALKAASAKDVLVRLGERRNAVTRFANNAITQNTLDAGTELAVEVA
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEEEE
FENRRASARVSSLSEEAIRTAVRRAEELARVARPDPEYLPPLPRQQYLAVDAYDPATANL
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCC
TPVERAKRAGRIAGAAGRASLRAAGTVESAERVSVVAASSGLFAYHLRTDAQIGCTLQGP
CHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHEEEEEECCCEEEEEEECCCEEEEEEECC
DSSGWARDVASRMALLSPERIAQSAIEQARRGVNPVAVEPGRYTVILAPAAVGTLLFHLV
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEECCCCEEEEECHHHHHHHHHHHH
EQMDARDTLDGVTFLSGKMGRQLVGSNITLRTDPANTRLPGVPFDEEGLPQPQLPWIDRG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHH
VFQQMRWDRFTAQKNNRPPVPAPRSLVMEGEDRSIEDLIRSTERGLLVTHVWYVRDVKPD
HHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCHHHHHHHHCCCEEEEEEEEEEECCCC
ETTVTGLTRDGTFLIEKGQLTRGVKVLRFNQSLLAMLSSTLALSRPEAAADNELPPSLLP
CCEEEEEECCCEEEEECCCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
ALKVAEFNFVSGSSF
HHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]