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The map label for this gene is kefA [H]

Identifier: 37521105

GI number: 37521105

Start: 1657861

End: 1659117

Strand: Reverse

Name: kefA [H]

Synonym: gll1536

Alternate gene names: 37521105

Gene position: 1659117-1657861 (Counterclockwise)

Preceding gene: 37521108

Following gene: 37521102

Centisome position: 35.61

GC content: 63.09

Gene sequence:

>1257_bases
GTGCTCTACACTTGGGACATGCGCAGGCGGCATTGCTATTCTCTTTTAGCGCAGGCGGCGGGGCTCTGGGGGGCGCTCGC
CTTACCGGCCGGGGCGCAGAGCGAGATGCCGCTCATCACCGTGCCCCTCGAAGCGCTGCAGTGGTTGATGGCCGTCGCTT
CGGTGGTCCTGGGAGCGCTGGGCAGTTGGGGACTGAATATTGTTCGCGGCCGCTTGAAGTTTTTTATCGGCGACCAGCGC
TTCGACCCGTGGTTGAGGAGCCTGTTTGGCCTGCTGCAGGGGGGAGTGTGGTTTGCGGCCCTCTGGCTGGCCGCCGGAAA
CCTCAAAGGTCTTGATCCATTGCGCTCCCAGGTGGCATTGCTGCTGGGGGGGGTGGCCGATTTTTTGGTCGGGGTGTGGC
AGGTGCCGGTGCTCACCATCGACAAGACGCGCTTTCCCCTGGGTACGCTGCTGTTGCTGTTGGTGCTCGCTTTTGCCGTC
TTCTGGGCCTCGCGCCTCTCGGCGGTGGTGCTCAAGCGCTTCGTGCTCAGCCGCTTCAACCTGACCATCGGCAGCCAGGA
GGCGATCGCCACCGTCTGCAACTACGTGATCAGCGCCGTCGGCTACATTTTGCTGTTGCAGCTGGCAGGTATCGATCTCG
GCTCGATCGCCATTTTGCTGGGGGTGATCGGCCTGGGTGTGGGCTTCGCCCTGCAGAACCTGGCGCGCAACTTCATCAGC
GGACTGGTGTTGCTGATTGAGCGGCCCGTGCAGGTGGGCGACTACATCGTCATCGACAACAAAGACGGCGTCGTCGAAAG
CGTCAACCTGCGCGCCGCCACCTTGCGCCGCTTCGACGGCTCGCGGCTGATCGTTCCCAATACCCTGCTGGTCGATCAGC
AGGTGCTCAACTGGTCCACCGCCGAGAACCGGGCTCGCCTGTCCCTCGAAGTACTGGTACGCGGCAGCGGCGATCCGGAG
GTGGTGACGGAGACGCTGCTTAAAATTGCCTCCGAGGAGCGGCGCGTGCTCACCAATCCTGTTCCGGAAGTGATCTTCCG
GGGATGGCGCGAGTTGGGGATGCTCTTTGAGCTGTGGGCCTGGTGCGGTACTCCTAAGGAGCAACTGCGCATCAAAAGCG
CCCTCTACTACAAAATCGACGCCGAGCTGCGCGAGCGCGGCGTCGAGCTGGCCTTTCTGGAGTACGGCCAGCCGTGGCAA
ATGCTTCCGAACGGGACAACGCCGATGTCGATGCAGCAGCCCAACGGCCGCAACTGA

Upstream 100 bases:

>100_bases
GCGGGTCGAGGCCGACGGCCCGGATGCGTTCGGCGGGATCCCCTTTGCGTTGAACGATCACACGAGCCTCCGGAAGAACC
GCCGCAGGCGTCCCGGGAAC

Downstream 100 bases:

>100_bases
GCGGACCTGCGCCCGTCAATCCAGCTCCAAAGTCCGAAAGCTGTCTACCTGCAAGTACTCCCCAGACCAGGATGTCTGCC
GGCCATCCACCCGTGCGCGC

Product: mechanosensitive channel protein

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MLYTWDMRRRHCYSLLAQAAGLWGALALPAGAQSEMPLITVPLEALQWLMAVASVVLGALGSWGLNIVRGRLKFFIGDQR
FDPWLRSLFGLLQGGVWFAALWLAAGNLKGLDPLRSQVALLLGGVADFLVGVWQVPVLTIDKTRFPLGTLLLLLVLAFAV
FWASRLSAVVLKRFVLSRFNLTIGSQEAIATVCNYVISAVGYILLLQLAGIDLGSIAILLGVIGLGVGFALQNLARNFIS
GLVLLIERPVQVGDYIVIDNKDGVVESVNLRAATLRRFDGSRLIVPNTLLVDQQVLNWSTAENRARLSLEVLVRGSGDPE
VVTETLLKIASEERRVLTNPVPEVIFRGWRELGMLFELWAWCGTPKEQLRIKSALYYKIDAELRERGVELAFLEYGQPWQ
MLPNGTTPMSMQQPNGRN

Sequences:

>Translated_418_residues
MLYTWDMRRRHCYSLLAQAAGLWGALALPAGAQSEMPLITVPLEALQWLMAVASVVLGALGSWGLNIVRGRLKFFIGDQR
FDPWLRSLFGLLQGGVWFAALWLAAGNLKGLDPLRSQVALLLGGVADFLVGVWQVPVLTIDKTRFPLGTLLLLLVLAFAV
FWASRLSAVVLKRFVLSRFNLTIGSQEAIATVCNYVISAVGYILLLQLAGIDLGSIAILLGVIGLGVGFALQNLARNFIS
GLVLLIERPVQVGDYIVIDNKDGVVESVNLRAATLRRFDGSRLIVPNTLLVDQQVLNWSTAENRARLSLEVLVRGSGDPE
VVTETLLKIASEERRVLTNPVPEVIFRGWRELGMLFELWAWCGTPKEQLRIKSALYYKIDAELRERGVELAFLEYGQPWQ
MLPNGTTPMSMQQPNGRN
>Mature_418_residues
MLYTWDMRRRHCYSLLAQAAGLWGALALPAGAQSEMPLITVPLEALQWLMAVASVVLGALGSWGLNIVRGRLKFFIGDQR
FDPWLRSLFGLLQGGVWFAALWLAAGNLKGLDPLRSQVALLLGGVADFLVGVWQVPVLTIDKTRFPLGTLLLLLVLAFAV
FWASRLSAVVLKRFVLSRFNLTIGSQEAIATVCNYVISAVGYILLLQLAGIDLGSIAILLGVIGLGVGFALQNLARNFIS
GLVLLIERPVQVGDYIVIDNKDGVVESVNLRAATLRRFDGSRLIVPNTLLVDQQVLNWSTAENRARLSLEVLVRGSGDPE
VVTETLLKIASEERRVLTNPVPEVIFRGWRELGMLFELWAWCGTPKEQLRIKSALYYKIDAELRERGVELAFLEYGQPWQ
MLPNGTTPMSMQQPNGRN

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=243, Percent_Identity=34.9794238683128, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI2367355, Length=255, Percent_Identity=29.4117647058824, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789291, Length=225, Percent_Identity=26.6666666666667, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 46192; Mature: 46192

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYTWDMRRRHCYSLLAQAAGLWGALALPAGAQSEMPLITVPLEALQWLMAVASVVLGAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHH
GSWGLNIVRGRLKFFIGDQRFDPWLRSLFGLLQGGVWFAALWLAAGNLKGLDPLRSQVAL
HHCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHH
LLGGVADFLVGVWQVPVLTIDKTRFPLGTLLLLLVLAFAVFWASRLSAVVLKRFVLSRFN
HHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LTIGSQEAIATVCNYVISAVGYILLLQLAGIDLGSIAILLGVIGLGVGFALQNLARNFIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVLLIERPVQVGDYIVIDNKDGVVESVNLRAATLRRFDGSRLIVPNTLLVDQQVLNWST
HHHHHHHCCCCCCCEEEEECCCCCEEECCHHHHHHHHCCCCEEECCCHHHHHHHHHCCCC
AENRARLSLEVLVRGSGDPEVVTETLLKIASEERRVLTNPVPEVIFRGWRELGMLFELWA
CCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
WCGTPKEQLRIKSALYYKIDAELRERGVELAFLEYGQPWQMLPNGTTPMSMQQPNGRN
HHCCCHHHHHHHHHHHEEECHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLYTWDMRRRHCYSLLAQAAGLWGALALPAGAQSEMPLITVPLEALQWLMAVASVVLGAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHH
GSWGLNIVRGRLKFFIGDQRFDPWLRSLFGLLQGGVWFAALWLAAGNLKGLDPLRSQVAL
HHCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHH
LLGGVADFLVGVWQVPVLTIDKTRFPLGTLLLLLVLAFAVFWASRLSAVVLKRFVLSRFN
HHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LTIGSQEAIATVCNYVISAVGYILLLQLAGIDLGSIAILLGVIGLGVGFALQNLARNFIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVLLIERPVQVGDYIVIDNKDGVVESVNLRAATLRRFDGSRLIVPNTLLVDQQVLNWST
HHHHHHHCCCCCCCEEEEECCCCCEEECCHHHHHHHHCCCCEEECCCHHHHHHHHHCCCC
AENRARLSLEVLVRGSGDPEVVTETLLKIASEERRVLTNPVPEVIFRGWRELGMLFELWA
CCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
WCGTPKEQLRIKSALYYKIDAELRERGVELAFLEYGQPWQMLPNGTTPMSMQQPNGRN
HHCCCHHHHHHHHHHHEEECHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]