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The map label for this gene is gatA

Identifier: 37521097

GI number: 37521097

Start: 1648275

End: 1649744

Strand: Reverse

Name: gatA

Synonym: gll1528

Alternate gene names: 37521097

Gene position: 1649744-1648275 (Counterclockwise)

Preceding gene: 37521100

Following gene: 37521090

Centisome position: 35.41

GC content: 64.29

Gene sequence:

>1470_bases
ATGACATCGATCGGACAGCTGCGCTCGCAGGTCGCAAGTAAAGAGCGCTCGGCTGTGGAAGTGGCCAGGCAGTACCTGGA
GCGGGCCGAAAGACTCGACACGGAGGTCCATGCCTTTTTGCGCCTCACCCCAGAGCGCGCCCTCGCAGCAGCTGAAGCCG
TCGATGCAAAAATCGCCAGAGGCGAAGATCCGGGCCTGCTCGCCGGGGTGCCGGTCGCGGTCAAGGACAACCTGTGCATG
GTGGGCATTCCCACCACCTGTGCTTCCAAAATTCTCGAAAACTACCGCCCGCCCTACGAATCGACCGTGACCCGCCGGTT
GGAGGAGCAGGGGGCGCTCATCATCGGCAAGACCAACCTCGATGAATTTGCGATGGGCTCCTCGACCGAAAATTCCGCCT
TCGGCCCGACGCGCAATCCCTGGGATCTGGGCCGCGTGCCGGGGGGCTCCTCGGGCGGTTCGGCGGCGGCGGTGGCAGCC
TGCGAGGCGGTGGCCTCCCTGGGATCCGATACTGGCGGCTCGATCCGCCAACCCGCATCTTTTTGCGGCGTGGTCGGCCT
CAAGCCCACCTACGGGCTGGTCTCACGCTACGGCCTGATCGCCTTTGCCTCCTCGCTCGACCAAATTGGTCCCTTTACCC
GGACGGTGGAGGATGCCGCCCTGGTGCTGCAGGCGATCGCGGGCCACGACCCCCTCGATTCGACCAGTCTGGCGGTGAAC
GTGCCCGATTATCGTCAGGCCCTGATAAGCGACCTCAAGGGCGTAAAGGTGGGTTTCGTCAAAGAATTTTTTGCCGAGGG
TCTCGATCCCGACGTGGCCGACGCCGTCTTCGAGGCAATCGAGGTGATGCGGGATCTGGGAGCCCAGATTCAGGAAGTGA
GCTGCCCGCGCTTTGCCCGGGGACTGTCGACTTATTACATCATTGCCACCTCCGAGGCGTCGGCCAACCTGGCGCGCTAC
GACGGCGTCAAATACGGTCTGCGCGACCGGGAGGCGGACGCGCTGGTGCCGATGTACGGACGGACCCGCGAGGAAGGGTT
CGGCAGCGAAGTGAAGCGCCGCATCATGCTGGGCACCTACGCGCTGTCGGCCGGTTACTACGACGCCTATTACTTAAAAG
CCCAGAAGGTGCGCACGCTCATCAAGCAGGACTACCTGGACGCCTTCGCCAAAGTCGACGTGCTGGTCGGTCCTACCGCT
CCTACCACTGCCTTCGCCTTCGGCGACAAGGTGAGCGACCCGCTCAGCATGTACCTCTCGGACATTTACACCATCCCCCT
CAACCTGGCGGGGGTGGCCGGGGCGAGCATCCCCTGCGGCTTCGACGCCAAGGGCCTGCCCATCGGCTTTCAGATCATGG
CGAACGCCCTGGAGGAGGGCAAGCTCTTGCGGGCCGCCTACGCCTACGAGCAGGCCACCGAATGGCACAAACGCACCCCG
GCCCTGGCGGCAGAAGCGCTCACTCGCTGA

Upstream 100 bases:

>100_bases
CCACCATACTATGAGTTTGAATCGGACAAACGGTGTCGATTGTTCCAGAATCGGGGCCAAATCGGTACGATGGTTGTCTG
TGTGGACAGGAAATATGCAA

Downstream 100 bases:

>100_bases
GCAAAAAGATCAAAGGCCTGACGTCCTCTTCGCCTGGGGTCCATTCCCAATAGCGCACCAGGATCTGGTAGACCATCAGG
CCGGTCTCCCCCTGGCCGGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTSIGQLRSQVASKERSAVEVARQYLERAERLDTEVHAFLRLTPERALAAAEAVDAKIARGEDPGLLAGVPVAVKDNLCM
VGIPTTCASKILENYRPPYESTVTRRLEEQGALIIGKTNLDEFAMGSSTENSAFGPTRNPWDLGRVPGGSSGGSAAAVAA
CEAVASLGSDTGGSIRQPASFCGVVGLKPTYGLVSRYGLIAFASSLDQIGPFTRTVEDAALVLQAIAGHDPLDSTSLAVN
VPDYRQALISDLKGVKVGFVKEFFAEGLDPDVADAVFEAIEVMRDLGAQIQEVSCPRFARGLSTYYIIATSEASANLARY
DGVKYGLRDREADALVPMYGRTREEGFGSEVKRRIMLGTYALSAGYYDAYYLKAQKVRTLIKQDYLDAFAKVDVLVGPTA
PTTAFAFGDKVSDPLSMYLSDIYTIPLNLAGVAGASIPCGFDAKGLPIGFQIMANALEEGKLLRAAYAYEQATEWHKRTP
ALAAEALTR

Sequences:

>Translated_489_residues
MTSIGQLRSQVASKERSAVEVARQYLERAERLDTEVHAFLRLTPERALAAAEAVDAKIARGEDPGLLAGVPVAVKDNLCM
VGIPTTCASKILENYRPPYESTVTRRLEEQGALIIGKTNLDEFAMGSSTENSAFGPTRNPWDLGRVPGGSSGGSAAAVAA
CEAVASLGSDTGGSIRQPASFCGVVGLKPTYGLVSRYGLIAFASSLDQIGPFTRTVEDAALVLQAIAGHDPLDSTSLAVN
VPDYRQALISDLKGVKVGFVKEFFAEGLDPDVADAVFEAIEVMRDLGAQIQEVSCPRFARGLSTYYIIATSEASANLARY
DGVKYGLRDREADALVPMYGRTREEGFGSEVKRRIMLGTYALSAGYYDAYYLKAQKVRTLIKQDYLDAFAKVDVLVGPTA
PTTAFAFGDKVSDPLSMYLSDIYTIPLNLAGVAGASIPCGFDAKGLPIGFQIMANALEEGKLLRAAYAYEQATEWHKRTP
ALAAEALTR
>Mature_488_residues
TSIGQLRSQVASKERSAVEVARQYLERAERLDTEVHAFLRLTPERALAAAEAVDAKIARGEDPGLLAGVPVAVKDNLCMV
GIPTTCASKILENYRPPYESTVTRRLEEQGALIIGKTNLDEFAMGSSTENSAFGPTRNPWDLGRVPGGSSGGSAAAVAAC
EAVASLGSDTGGSIRQPASFCGVVGLKPTYGLVSRYGLIAFASSLDQIGPFTRTVEDAALVLQAIAGHDPLDSTSLAVNV
PDYRQALISDLKGVKVGFVKEFFAEGLDPDVADAVFEAIEVMRDLGAQIQEVSCPRFARGLSTYYIIATSEASANLARYD
GVKYGLRDREADALVPMYGRTREEGFGSEVKRRIMLGTYALSAGYYDAYYLKAQKVRTLIKQDYLDAFAKVDVLVGPTAP
TTAFAFGDKVSDPLSMYLSDIYTIPLNLAGVAGASIPCGFDAKGLPIGFQIMANALEEGKLLRAAYAYEQATEWHKRTPA
LAAEALTR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=465, Percent_Identity=38.9247311827957, Blast_Score=325, Evalue=4e-89,
Organism=Homo sapiens, GI195972892, Length=489, Percent_Identity=26.3803680981595, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI166795287, Length=240, Percent_Identity=30.8333333333333, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=407, Percent_Identity=40.2948402948403, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=508, Percent_Identity=25.7874015748031, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17556264, Length=304, Percent_Identity=31.9078947368421, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17556276, Length=303, Percent_Identity=31.023102310231, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17556278, Length=303, Percent_Identity=31.023102310231, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71990152, Length=340, Percent_Identity=27.6470588235294, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=312, Percent_Identity=26.9230769230769, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17538254, Length=323, Percent_Identity=22.9102167182663, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=461, Percent_Identity=33.6225596529284, Blast_Score=242, Evalue=9e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=423, Percent_Identity=30.0236406619385, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=237, Percent_Identity=29.1139240506329, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24648113, Length=492, Percent_Identity=40.650406504065, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24652985, Length=501, Percent_Identity=25.5489021956088, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI19922090, Length=501, Percent_Identity=25.5489021956088, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24652981, Length=501, Percent_Identity=25.5489021956088, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24652983, Length=501, Percent_Identity=25.5489021956088, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI161078093, Length=510, Percent_Identity=25.8823529411765, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24644968, Length=516, Percent_Identity=25.5813953488372, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI21356731, Length=480, Percent_Identity=26.0416666666667, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI45550774, Length=482, Percent_Identity=24.2738589211618, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24648435, Length=482, Percent_Identity=24.2738589211618, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24648437, Length=482, Percent_Identity=24.2738589211618, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24648441, Length=419, Percent_Identity=24.8210023866348, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648439, Length=419, Percent_Identity=24.8210023866348, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_GLOVI (Q7NKF0)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_924474.1
- ProteinModelPortal:   Q7NKF0
- SMR:   Q7NKF0
- GeneID:   2598602
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll1528
- NMPDR:   fig|251221.1.peg.1528
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   GVIO251221:GLL1528-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52304; Mature: 52173

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 75-75 ACT_SITE 150-150 ACT_SITE 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIGQLRSQVASKERSAVEVARQYLERAERLDTEVHAFLRLTPERALAAAEAVDAKIAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
GEDPGLLAGVPVAVKDNLCMVGIPTTCASKILENYRPPYESTVTRRLEEQGALIIGKTNL
CCCCCEEECCCEEEECCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCC
DEFAMGSSTENSAFGPTRNPWDLGRVPGGSSGGSAAAVAACEAVASLGSDTGGSIRQPAS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHH
FCGVVGLKPTYGLVSRYGLIAFASSLDQIGPFTRTVEDAALVLQAIAGHDPLDSTSLAVN
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
VPDYRQALISDLKGVKVGFVKEFFAEGLDPDVADAVFEAIEVMRDLGAQIQEVSCPRFAR
CCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
GLSTYYIIATSEASANLARYDGVKYGLRDREADALVPMYGRTREEGFGSEVKRRIMLGTY
CCCEEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ALSAGYYDAYYLKAQKVRTLIKQDYLDAFAKVDVLVGPTAPTTAFAFGDKVSDPLSMYLS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHCCCCCCHHHHHHHH
DIYTIPLNLAGVAGASIPCGFDAKGLPIGFQIMANALEEGKLLRAAYAYEQATEWHKRTP
HHHHCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCH
ALAAEALTR
HHHHHHHCC
>Mature Secondary Structure 
TSIGQLRSQVASKERSAVEVARQYLERAERLDTEVHAFLRLTPERALAAAEAVDAKIAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
GEDPGLLAGVPVAVKDNLCMVGIPTTCASKILENYRPPYESTVTRRLEEQGALIIGKTNL
CCCCCEEECCCEEEECCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCC
DEFAMGSSTENSAFGPTRNPWDLGRVPGGSSGGSAAAVAACEAVASLGSDTGGSIRQPAS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHH
FCGVVGLKPTYGLVSRYGLIAFASSLDQIGPFTRTVEDAALVLQAIAGHDPLDSTSLAVN
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
VPDYRQALISDLKGVKVGFVKEFFAEGLDPDVADAVFEAIEVMRDLGAQIQEVSCPRFAR
CCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
GLSTYYIIATSEASANLARYDGVKYGLRDREADALVPMYGRTREEGFGSEVKRRIMLGTY
CCCEEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ALSAGYYDAYYLKAQKVRTLIKQDYLDAFAKVDVLVGPTAPTTAFAFGDKVSDPLSMYLS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHCCCCCCHHHHHHHH
DIYTIPLNLAGVAGASIPCGFDAKGLPIGFQIMANALEEGKLLRAAYAYEQATEWHKRTP
HHHHCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCH
ALAAEALTR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292