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The map label for this gene is azoB [H]

Identifier: 37521029

GI number: 37521029

Start: 1565783

End: 1566661

Strand: Direct

Name: azoB [H]

Synonym: glr1460

Alternate gene names: 37521029

Gene position: 1565783-1566661 (Clockwise)

Preceding gene: 37521028

Following gene: 37521030

Centisome position: 33.61

GC content: 60.75

Gene sequence:

>879_bases
ATGTTCTTGGTCACAGGAGCCACGGGCGATCTCGGTCGGCGAATTGTCCGGTCTTTGCGCGGGCGCGGCCAACCGGTCAG
GGCCTTCGTGCGCTTGGAAGCCCGCTACGCCGACCTCGAACAGATGGGTGCAGAAATCTTCATCGGCGATCTTCGCCGCC
GCGATCTGATCGAGCGGGCCGTCCGCGGCGCGCGCTACGTCATCAGCGCCCACGGCACCCGGCCCGGCCAGAGCATCGCC
GAGGTCGAATACCAGGCCAACATCGACCTCATCGAAGCGGCCCAAACACAAGGAGTCGAACGCTTCGTCTACATTTCGGT
CCTCGGGGCCGATCGCCACTACGACGACGCCCCTGTCTTCAAGGCCAAGCGCGAAGTCGAGAAATACCTGACCCGTACCC
CGATCCCGTACACCGTTTTGCGACCGGCAGGTTTTGCCTCCAACTTGCTCACACTTGCACGCAATTTTGAGCGCACCGGG
TTCTATTTTTTGATCGGTCGGCGCGAAAACCGTACATCGCTGGTCAGTACCGACGACCTGAGCGAGATCGCTATCCAGGC
AGCGAGCCTGCCGGAAGCCCGCAATCGAACTTTTGCCATCGGCGGCCCCGAATCGCTCCGCCGCGACGAGATCCCCAAAA
TCTTCGAAAAGCTATTCAACCGCGCCGGCCAGATTCTGCAACTTCCTATCGAAGCCTTCGATGCGGCCCGCACGTTGATC
GGTCTGGTCAATCCGGTCATCAGCCGGGATCTCGGCACCTTGCGGGTGTTGCTGGCCAACGAATACACTTGCGACCCCCA
CGAGGTCCAGCAGGTCTTCCAGATCGAACTGGAGTCTTTGCACCACTTTTTGCGCCGCAACCTCCTGTACTCCACCTAA

Upstream 100 bases:

>100_bases
TGGGTGCGCCGAAAAGACATCCCCCCCTACAACTATTTGGGCTAGCATTTCGGTAATTCTTCCGGTTCTGACCAGCGGAA
GGGCGACAAAGGGGCGCAGT

Downstream 100 bases:

>100_bases
ATGGCCAATTCGCCCGTCTACGAACGCATCTACGCTGTAGTCCGCCGTATCCCCGCGGGCAAAGTGGCCACCTACGGCCA
GGTGGCACTTTGGGCAGGCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MFLVTGATGDLGRRIVRSLRGRGQPVRAFVRLEARYADLEQMGAEIFIGDLRRRDLIERAVRGARYVISAHGTRPGQSIA
EVEYQANIDLIEAAQTQGVERFVYISVLGADRHYDDAPVFKAKREVEKYLTRTPIPYTVLRPAGFASNLLTLARNFERTG
FYFLIGRRENRTSLVSTDDLSEIAIQAASLPEARNRTFAIGGPESLRRDEIPKIFEKLFNRAGQILQLPIEAFDAARTLI
GLVNPVISRDLGTLRVLLANEYTCDPHEVQQVFQIELESLHHFLRRNLLYST

Sequences:

>Translated_292_residues
MFLVTGATGDLGRRIVRSLRGRGQPVRAFVRLEARYADLEQMGAEIFIGDLRRRDLIERAVRGARYVISAHGTRPGQSIA
EVEYQANIDLIEAAQTQGVERFVYISVLGADRHYDDAPVFKAKREVEKYLTRTPIPYTVLRPAGFASNLLTLARNFERTG
FYFLIGRRENRTSLVSTDDLSEIAIQAASLPEARNRTFAIGGPESLRRDEIPKIFEKLFNRAGQILQLPIEAFDAARTLI
GLVNPVISRDLGTLRVLLANEYTCDPHEVQQVFQIELESLHHFLRRNLLYST
>Mature_292_residues
MFLVTGATGDLGRRIVRSLRGRGQPVRAFVRLEARYADLEQMGAEIFIGDLRRRDLIERAVRGARYVISAHGTRPGQSIA
EVEYQANIDLIEAAQTQGVERFVYISVLGADRHYDDAPVFKAKREVEKYLTRTPIPYTVLRPAGFASNLLTLARNFERTG
FYFLIGRRENRTSLVSTDDLSEIAIQAASLPEARNRTFAIGGPESLRRDEIPKIFEKLFNRAGQILQLPIEAFDAARTLI
GLVNPVISRDLGTLRVLLANEYTCDPHEVQQVFQIELESLHHFLRRNLLYST

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 33073; Mature: 33073

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLVTGATGDLGRRIVRSLRGRGQPVRAFVRLEARYADLEQMGAEIFIGDLRRRDLIERA
CEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHH
VRGARYVISAHGTRPGQSIAEVEYQANIDLIEAAQTQGVERFVYISVLGADRHYDDAPVF
HCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHCCCEEEEEEEEEECCCCCCCCHHH
KAKREVEKYLTRTPIPYTVLRPAGFASNLLTLARNFERTGFYFLIGRRENRTSLVSTDDL
HHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCEECCCCH
SEIAIQAASLPEARNRTFAIGGPESLRRDEIPKIFEKLFNRAGQILQLPIEAFDAARTLI
HHHHHHHHCCCCCCCCEEEECCHHHHCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
GLVNPVISRDLGTLRVLLANEYTCDPHEVQQVFQIELESLHHFLRRNLLYST
HHHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFLVTGATGDLGRRIVRSLRGRGQPVRAFVRLEARYADLEQMGAEIFIGDLRRRDLIERA
CEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHH
VRGARYVISAHGTRPGQSIAEVEYQANIDLIEAAQTQGVERFVYISVLGADRHYDDAPVF
HCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHCCCEEEEEEEEEECCCCCCCCHHH
KAKREVEKYLTRTPIPYTVLRPAGFASNLLTLARNFERTGFYFLIGRRENRTSLVSTDDL
HHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCEECCCCH
SEIAIQAASLPEARNRTFAIGGPESLRRDEIPKIFEKLFNRAGQILQLPIEAFDAARTLI
HHHHHHHHCCCCCCCCEEEECCHHHHCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
GLVNPVISRDLGTLRVLLANEYTCDPHEVQQVFQIELESLHHFLRRNLLYST
HHHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]