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The map label for this gene is rluE [H]

Identifier: 37520948

GI number: 37520948

Start: 1477520

End: 1478077

Strand: Reverse

Name: rluE [H]

Synonym: gll1379

Alternate gene names: 37520948

Gene position: 1478077-1477520 (Counterclockwise)

Preceding gene: 37520953

Following gene: 37520947

Centisome position: 31.73

GC content: 67.2

Gene sequence:

>558_bases
GTGCTCACCCAATTTAGCGGTGGAGAAGGACGGCATACGCTCAAAGATTATATAGATATTCCCGGCGTTTACCCGGTGGG
GCGGCTCGACCGCGACAGCGAAGGGCTGCTGCTGTTGACCGACGACGGCGCCCTGGCCCATCGGCTGACCGATCCGCGCT
TCGCCCACCCGCGCACCTACTGGGTGCAGGTGGAAGGCACACCGGACGGCGAAGCGCTGGGGCGCCTCGCGGCGGGAGTC
GAGATCCAGGGGATACGCACCCGCCCGGCGACAGCTGTGGCGATTGCCGAGCCGGACCTGCCGGAGCGCCCGGTGCCCAT
CCGGGTGCGCAAGAGCATTCCCACCAGTTGGATAGCGCTCACCCTCACCGAGGGCCGCAACCGCCAGGTGCGGCGGATGA
CGGCGGCGGTGGGCTATGCGACCCTCAGGCTGGTTAGGGTGGCGATCGGTGCGCTCAGCCTGGGCGATCTCGCCCCCGGC
CGGTGGCGGGAGCTGAGCGAACGGGAGTTACGGTTGATAACATCGAAGCCTAGCCGGTCCCACATCGGGAGATTTTGA

Upstream 100 bases:

>100_bases
TGGCCGTAACGTTTTGGTGAATTGATAATGGTTTGCAGGTCAAAACACTTGTACAGGAGGGGTTCCGCTGCGCTATCTGC
TTTTCAATAAGCCCTACGAC

Downstream 100 bases:

>100_bases
CAATGCAGAGCCTGGCACAAGATTTCTCCTCCTGCAGCCTGCTCAGGAACGTTAGTTGGGAGACTTTTGAGCGGCTGTTG
GCCGAACTGCCCGAGACGCG

Product: pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MLTQFSGGEGRHTLKDYIDIPGVYPVGRLDRDSEGLLLLTDDGALAHRLTDPRFAHPRTYWVQVEGTPDGEALGRLAAGV
EIQGIRTRPATAVAIAEPDLPERPVPIRVRKSIPTSWIALTLTEGRNRQVRRMTAAVGYATLRLVRVAIGALSLGDLAPG
RWRELSERELRLITSKPSRSHIGRF

Sequences:

>Translated_185_residues
MLTQFSGGEGRHTLKDYIDIPGVYPVGRLDRDSEGLLLLTDDGALAHRLTDPRFAHPRTYWVQVEGTPDGEALGRLAAGV
EIQGIRTRPATAVAIAEPDLPERPVPIRVRKSIPTSWIALTLTEGRNRQVRRMTAAVGYATLRLVRVAIGALSLGDLAPG
RWRELSERELRLITSKPSRSHIGRF
>Mature_185_residues
MLTQFSGGEGRHTLKDYIDIPGVYPVGRLDRDSEGLLLLTDDGALAHRLTDPRFAHPRTYWVQVEGTPDGEALGRLAAGV
EIQGIRTRPATAVAIAEPDLPERPVPIRVRKSIPTSWIALTLTEGRNRQVRRMTAAVGYATLRLVRVAIGALSLGDLAPG
RWRELSERELRLITSKPSRSHIGRF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=168, Percent_Identity=55.952380952381, Blast_Score=169, Evalue=8e-44,
Organism=Escherichia coli, GI1788510, Length=160, Percent_Identity=37.5, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1790453, Length=161, Percent_Identity=32.2981366459627, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1787524, Length=142, Percent_Identity=39.4366197183099, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 20434; Mature: 20434

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQFSGGEGRHTLKDYIDIPGVYPVGRLDRDSEGLLLLTDDGALAHRLTDPRFAHPRTY
CCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHHCCCCCCCCCEEE
WVQVEGTPDGEALGRLAAGVEIQGIRTRPATAVAIAEPDLPERPVPIRVRKSIPTSWIAL
EEEEECCCCHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCCCCCCEEEECCCCCEEEEE
TLTEGRNRQVRRMTAAVGYATLRLVRVAIGALSLGDLAPGRWRELSERELRLITSKPSRS
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEECCCCHH
HIGRF
CCCCC
>Mature Secondary Structure
MLTQFSGGEGRHTLKDYIDIPGVYPVGRLDRDSEGLLLLTDDGALAHRLTDPRFAHPRTY
CCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHHCCCCCCCCCEEE
WVQVEGTPDGEALGRLAAGVEIQGIRTRPATAVAIAEPDLPERPVPIRVRKSIPTSWIAL
EEEEECCCCHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCCCCCCEEEECCCCCEEEEE
TLTEGRNRQVRRMTAAVGYATLRLVRVAIGALSLGDLAPGRWRELSERELRLITSKPSRS
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEECCCCHH
HIGRF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]