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The map label for this gene is gdhA [H]

Identifier: 37520702

GI number: 37520702

Start: 1212236

End: 1213612

Strand: Reverse

Name: gdhA [H]

Synonym: gvip150

Alternate gene names: 37520702

Gene position: 1213612-1212236 (Counterclockwise)

Preceding gene: 37520703

Following gene: 37520701

Centisome position: 26.05

GC content: 65.07

Gene sequence:

>1377_bases
ATGTTACGTTCTCTGACGCTCGCTTGGGGCGGGGATCAATGTTTTTGTAACATTGAATACAGCTGTTTGAATCCATTCGT
CAAGGGGAGAAGCATGGTTACCAGCCTCAACGACTCGCCCAGTCCGGCTTATATTTGTCCGAACGACATGGCCTGCTCGA
ATCTCGACCGCGCCGCCCAGATTTTGCGCCTCGATCCGGGAGTGGTCGAGGTGTTGGGTACGCCGCACCGGGTGCTCACC
GTGTCGGTACCGGTGCGCATGGACAACGGCCGGATCCGGGTCTTCGCAGGCCACCGGGTGCAGCACTGCAACGTGCTGGG
TCCCTACAAAGGCGGCATGCGCTATCACCCGGACGTGACGCTTCGGGAAGTGTCGACCCTGGCGATGCTGATGACCTGGA
AGTGCGCACTGATGGGTATTCCCTACGGCGGGGCCAAAGGCGGCATTGCCGTCAACCCGACGACCCTTTCGGTGGGCGAA
CTGGAGCGGCTCACCCGCCGCTTTACCAGCGAACTGGTGCGCGACATCGGCCCGCAAATTGACATCCCGGCTCCGGATGT
GGGCACCGGACCGCGGGAGATGGCCTGGATGATGGACACCTACTCAATGAGTATCGGGCACGCCTCGCCGGGGGTGGTGA
CCGGCAAGCCCCTCAGCATCGGCGGCTCCAAGGGCCGCGACGCGGCCACCGGCAGGGGCGTGGTCATCGCCACGCGCGAG
GCGCTCGACACCGCCGGGCTGGCGCTGCGCGGCGCCACCATTGCCATTCAAGGATTCGGCAAAGTGGGCAAGGCGGCGGC
GCTTATCTTCCAGCAGCATGGAGCGCGGGTGATCGCGCTCTCCGACGGCTCCGGGGGCATCTACAACCCCGACGGTCTCG
ACATCGAGCAGGCCGCCGATTTTGTGCGCGACGGCAGCCGACTGGCCCAGTATCCGTTTCCAGGGGTCAAGCCGATCGCC
AACCCCGAACTGTTGACGCTGCCGTGCGACGTGCTGGTCCCCGCCGCCCTTGAACACCAGATCACCGAGGCAAACGCCGC
CCGGGTGCGCGCCCGTCTCGTGGTCGAAGCCGCCAACGCCCCCACGACGATGGAGGCCGACCGCATCCTGGAGGAGCGCG
GCGTGACGGTACTGCCGGATATCCTCGCCAACGCCGGGGGCGTGGTGGTGAGCTATCTCGAATGGGTGCAGGGGTTGTCG
TATCTTTTTTGGGACGAGGAGAAGGTCAACCTCGAACTGGAGAAGTTGATGGTGGGCGCCTACGCGCGCGTGCTGCAACA
GGCCACCCAGTTCCGATTGCCCCTCAGGCCCGCCGCCTACACCCTCGCCGTCGGGCGGGTGGTGGAGGCGTTCAACCACC
GCGGCCTCTACCCGTAA

Upstream 100 bases:

>100_bases
CACCGCCCAGGGCAACTTGGTGCCTATCAAGGCCGAGAGCCTCACCGACTGAAGGCCGACCCTCCCCTGTAAGGCTGCAA
AACTGTAGCAAAAGCTACCA

Downstream 100 bases:

>100_bases
AGGCCGTTGCCCTGCGGGGGGTGCCCTTTCTGCTAAAGTTGGAAGGGCAAAATCGCGGGGATGCGGGTGGTTTCGGTATT
GGGGCTGGACGTGGGCAGCA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MLRSLTLAWGGDQCFCNIEYSCLNPFVKGRSMVTSLNDSPSPAYICPNDMACSNLDRAAQILRLDPGVVEVLGTPHRVLT
VSVPVRMDNGRIRVFAGHRVQHCNVLGPYKGGMRYHPDVTLREVSTLAMLMTWKCALMGIPYGGAKGGIAVNPTTLSVGE
LERLTRRFTSELVRDIGPQIDIPAPDVGTGPREMAWMMDTYSMSIGHASPGVVTGKPLSIGGSKGRDAATGRGVVIATRE
ALDTAGLALRGATIAIQGFGKVGKAAALIFQQHGARVIALSDGSGGIYNPDGLDIEQAADFVRDGSRLAQYPFPGVKPIA
NPELLTLPCDVLVPAALEHQITEANAARVRARLVVEAANAPTTMEADRILEERGVTVLPDILANAGGVVVSYLEWVQGLS
YLFWDEEKVNLELEKLMVGAYARVLQQATQFRLPLRPAAYTLAVGRVVEAFNHRGLYP

Sequences:

>Translated_458_residues
MLRSLTLAWGGDQCFCNIEYSCLNPFVKGRSMVTSLNDSPSPAYICPNDMACSNLDRAAQILRLDPGVVEVLGTPHRVLT
VSVPVRMDNGRIRVFAGHRVQHCNVLGPYKGGMRYHPDVTLREVSTLAMLMTWKCALMGIPYGGAKGGIAVNPTTLSVGE
LERLTRRFTSELVRDIGPQIDIPAPDVGTGPREMAWMMDTYSMSIGHASPGVVTGKPLSIGGSKGRDAATGRGVVIATRE
ALDTAGLALRGATIAIQGFGKVGKAAALIFQQHGARVIALSDGSGGIYNPDGLDIEQAADFVRDGSRLAQYPFPGVKPIA
NPELLTLPCDVLVPAALEHQITEANAARVRARLVVEAANAPTTMEADRILEERGVTVLPDILANAGGVVVSYLEWVQGLS
YLFWDEEKVNLELEKLMVGAYARVLQQATQFRLPLRPAAYTLAVGRVVEAFNHRGLYP
>Mature_458_residues
MLRSLTLAWGGDQCFCNIEYSCLNPFVKGRSMVTSLNDSPSPAYICPNDMACSNLDRAAQILRLDPGVVEVLGTPHRVLT
VSVPVRMDNGRIRVFAGHRVQHCNVLGPYKGGMRYHPDVTLREVSTLAMLMTWKCALMGIPYGGAKGGIAVNPTTLSVGE
LERLTRRFTSELVRDIGPQIDIPAPDVGTGPREMAWMMDTYSMSIGHASPGVVTGKPLSIGGSKGRDAATGRGVVIATRE
ALDTAGLALRGATIAIQGFGKVGKAAALIFQQHGARVIALSDGSGGIYNPDGLDIEQAADFVRDGSRLAQYPFPGVKPIA
NPELLTLPCDVLVPAALEHQITEANAARVRARLVVEAANAPTTMEADRILEERGVTVLPDILANAGGVVVSYLEWVQGLS
YLFWDEEKVNLELEKLMVGAYARVLQQATQFRLPLRPAAYTLAVGRVVEAFNHRGLYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=352, Percent_Identity=44.0340909090909, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI4885281, Length=352, Percent_Identity=44.3181818181818, Blast_Score=305, Evalue=6e-83,
Organism=Escherichia coli, GI1788059, Length=360, Percent_Identity=36.3888888888889, Blast_Score=203, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17544676, Length=389, Percent_Identity=39.0745501285347, Blast_Score=276, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6319256, Length=391, Percent_Identity=32.7365728900256, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6324951, Length=396, Percent_Identity=31.3131313131313, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI45549226, Length=361, Percent_Identity=39.3351800554017, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI45553475, Length=361, Percent_Identity=39.3351800554017, Blast_Score=278, Evalue=4e-75,
Organism=Drosophila melanogaster, GI24649283, Length=383, Percent_Identity=36.8146214099217, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI24649460, Length=297, Percent_Identity=41.0774410774411, Blast_Score=238, Evalue=9e-63,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 49242; Mature: 49242

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRSLTLAWGGDQCFCNIEYSCLNPFVKGRSMVTSLNDSPSPAYICPNDMACSNLDRAAQ
CCCEEEEEECCCEEEEEEEHHHHCHHHHCCHHEEECCCCCCCCEECCCCCCCCCHHHHHH
ILRLDPGVVEVLGTPHRVLTVSVPVRMDNGRIRVFAGHRVQHCNVLGPYKGGMRYHPDVT
HHCCCCCCEEECCCCCEEEEEEEEEEECCCEEEEEECCCEEECCEECCCCCCCEECCCCH
LREVSTLAMLMTWKCALMGIPYGGAKGGIAVNPTTLSVGELERLTRRFTSELVRDIGPQI
HHHHHHHHHHHHHHHHHEECCCCCCCCCEEECCCEECHHHHHHHHHHHHHHHHHHCCCCC
DIPAPDVGTGPREMAWMMDTYSMSIGHASPGVVTGKPLSIGGSKGRDAATGRGVVIATRE
CCCCCCCCCCHHHHHEEHHHHEEECCCCCCCEECCCCEECCCCCCCCCCCCCEEEEEEHH
ALDTAGLALRGATIAIQGFGKVGKAAALIFQQHGARVIALSDGSGGIYNPDGLDIEQAAD
HHHHCCEEEECCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHH
FVRDGSRLAQYPFPGVKPIANPELLTLPCDVLVPAALEHQITEANAARVRARLVVEAANA
HHHCCCCEECCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCC
PTTMEADRILEERGVTVLPDILANAGGVVVSYLEWVQGLSYLFWDEEKVNLELEKLMVGA
CCCHHHHHHHHHCCCEEEHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHH
YARVLQQATQFRLPLRPAAYTLAVGRVVEAFNHRGLYP
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLRSLTLAWGGDQCFCNIEYSCLNPFVKGRSMVTSLNDSPSPAYICPNDMACSNLDRAAQ
CCCEEEEEECCCEEEEEEEHHHHCHHHHCCHHEEECCCCCCCCEECCCCCCCCCHHHHHH
ILRLDPGVVEVLGTPHRVLTVSVPVRMDNGRIRVFAGHRVQHCNVLGPYKGGMRYHPDVT
HHCCCCCCEEECCCCCEEEEEEEEEEECCCEEEEEECCCEEECCEECCCCCCCEECCCCH
LREVSTLAMLMTWKCALMGIPYGGAKGGIAVNPTTLSVGELERLTRRFTSELVRDIGPQI
HHHHHHHHHHHHHHHHHEECCCCCCCCCEEECCCEECHHHHHHHHHHHHHHHHHHCCCCC
DIPAPDVGTGPREMAWMMDTYSMSIGHASPGVVTGKPLSIGGSKGRDAATGRGVVIATRE
CCCCCCCCCCHHHHHEEHHHHEEECCCCCCCEECCCCEECCCCCCCCCCCCCEEEEEEHH
ALDTAGLALRGATIAIQGFGKVGKAAALIFQQHGARVIALSDGSGGIYNPDGLDIEQAAD
HHHHCCEEEECCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHH
FVRDGSRLAQYPFPGVKPIANPELLTLPCDVLVPAALEHQITEANAARVRARLVVEAANA
HHHCCCCEECCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCC
PTTMEADRILEERGVTVLPDILANAGGVVVSYLEWVQGLSYLFWDEEKVNLELEKLMVGA
CCCHHHHHHHHHCCCEEEHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHH
YARVLQQATQFRLPLRPAAYTLAVGRVVEAFNHRGLYP
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]