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The map label for this gene is lcfA [H]

Identifier: 37520691

GI number: 37520691

Start: 1202678

End: 1204192

Strand: Direct

Name: lcfA [H]

Synonym: glr1122

Alternate gene names: 37520691

Gene position: 1202678-1204192 (Clockwise)

Preceding gene: 37520690

Following gene: 37520693

Centisome position: 25.81

GC content: 55.91

Gene sequence:

>1515_bases
GTGAATCTTCCCCTGGTCCATCGTGCCTACGAACATGGCCCCAGAACTGCGATCGTTACCGCTGACGGGCAATTTTCCTA
TCAGGATCTGCTTAGCCTTTCCAGTCAGGTCGCAACCCGGCTTCTCGATGGTGCCGACGATTTGCGGGAGCAGCGGGTTG
CCTCTCTGGTGCCGGCAGGTTTTGAGTATGTCGCGACCCAGTGGGGTATCTGGCGGGCAGGCGGCATTGCAGTGCCCTTG
TGTGTTTCCCATCCACGGCCCGAACTGGAGTATGTGATTACCAACTCCGGCGCGTCCATTGTGATCGCCCATCCTAGTTT
TGAATCACTCCTACGTCCGATCGCCGAGGCACATCAACTGCGTTTTATCCTCACCTCCGAATCCCTGCCCCCTGACACAG
CGCAACTACCGGAAGTTGAGCCCACCAGACGCGCCTTGATTCTCTACACCAGCGGCACCACCGGAAAACCCAAGGGAGTC
GTGACAACCCACGGCAATATCCAGGCACAGGTCACTAGCCTGATTTCAGCCTGGGAATGGACATCCGGCGATCGCATCTT
GCATGTCCTGCCGTTGCATCACATTCACGGCATTGTCAATGTGTTGACTTGCGCGTTGTGGGCCGGTGCCGAGTGCCACA
TGCTCAGCAAATTTGATGCCGAGACCGTTTGGGAGCATATTTGCGAGGGAAACTTCACTCTGTTTATGGCGGTTCCCACC
ATCTACACAAAACTTATTGCGACTTGGGAAAAAGCCGCTGGCGACCGGCAAATGGATATGTCCAGAGGCTGCGCCGGGAT
GCGATTGATGGTAAGCGGCTCGGCGGCCCTACCTGTCCAAGTTCTAGAGCGATGGACGCACATCAGTGGCCACTTTCTGC
TGGAGCGCTACGGCATGACCGAAATTGGAATGGCACTCTCGAATCCGCTCCACGGTCAACGACGGGCAGGCTATGTCGGA
ATGGCTTTGCCTCAAGTGGACGTGAGATTGGTGGATGAAAGCGGAGTTTCTGTTCCGGCAGGAACGCCCGGAGAAATCCA
GGTGAAGGGCCCGGGTGTATTTTTGGAATATTGGCAGAAACCGGAAGCAACCGCCAAAGCCTTTCAAGATGGCTGGTTCT
GCACAGGCGATCTCGCTGTCGTTGAGCAAGATTGCTATCGCATTCTGGGGCGGATCAGCGTCGATATTATCAAAACCGGA
GGCTACAAAGTTTCAGCTTTGGAAATTGAGGAAGTCCTGCGCACCCATCCCGACATTCAGGAGTGCGCCGTGGTTGGGGT
GGCCGATCCGGAATGGGGAGAGCGGGTCTGTGGGGCATTGGTCCTGCAATCAGGCTGCAATTTGGCGTTAGAACCTTTCC
GCAGCTGGGCAAAAGAACGTCTGGCAGCTTACAAAGTGCCGACTCGAATCCTGAGTGTCGAAGAGTTACCCCGCAATGCG
ATGGGGAAAGTCACCAAGCCGGCCGTGGCACAACTCTTTCGCGCCCAACAAATTGCTGAATCGCAACCAGCATAG

Upstream 100 bases:

>100_bases
GTACTTACAGATAAATATTCTCTCGCACGTAGCTAGACTGGCGATCGTCCGCTATGCTCTCTGTAGTCGGTCTATATCTA
TCTTGCACCGGTCAATTTCT

Downstream 100 bases:

>100_bases
GCTGTCTACTCAGTGGTTCAGTTGCATGTGTGGAAATTCCCGGTTGCAAACGATCCCGCTGCCGCCAGGGGTGGTTCTGC
CCGCAGCACGACCAAAATCA

Product: long chain fatty acid CoA ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MNLPLVHRAYEHGPRTAIVTADGQFSYQDLLSLSSQVATRLLDGADDLREQRVASLVPAGFEYVATQWGIWRAGGIAVPL
CVSHPRPELEYVITNSGASIVIAHPSFESLLRPIAEAHQLRFILTSESLPPDTAQLPEVEPTRRALILYTSGTTGKPKGV
VTTHGNIQAQVTSLISAWEWTSGDRILHVLPLHHIHGIVNVLTCALWAGAECHMLSKFDAETVWEHICEGNFTLFMAVPT
IYTKLIATWEKAAGDRQMDMSRGCAGMRLMVSGSAALPVQVLERWTHISGHFLLERYGMTEIGMALSNPLHGQRRAGYVG
MALPQVDVRLVDESGVSVPAGTPGEIQVKGPGVFLEYWQKPEATAKAFQDGWFCTGDLAVVEQDCYRILGRISVDIIKTG
GYKVSALEIEEVLRTHPDIQECAVVGVADPEWGERVCGALVLQSGCNLALEPFRSWAKERLAAYKVPTRILSVEELPRNA
MGKVTKPAVAQLFRAQQIAESQPA

Sequences:

>Translated_504_residues
MNLPLVHRAYEHGPRTAIVTADGQFSYQDLLSLSSQVATRLLDGADDLREQRVASLVPAGFEYVATQWGIWRAGGIAVPL
CVSHPRPELEYVITNSGASIVIAHPSFESLLRPIAEAHQLRFILTSESLPPDTAQLPEVEPTRRALILYTSGTTGKPKGV
VTTHGNIQAQVTSLISAWEWTSGDRILHVLPLHHIHGIVNVLTCALWAGAECHMLSKFDAETVWEHICEGNFTLFMAVPT
IYTKLIATWEKAAGDRQMDMSRGCAGMRLMVSGSAALPVQVLERWTHISGHFLLERYGMTEIGMALSNPLHGQRRAGYVG
MALPQVDVRLVDESGVSVPAGTPGEIQVKGPGVFLEYWQKPEATAKAFQDGWFCTGDLAVVEQDCYRILGRISVDIIKTG
GYKVSALEIEEVLRTHPDIQECAVVGVADPEWGERVCGALVLQSGCNLALEPFRSWAKERLAAYKVPTRILSVEELPRNA
MGKVTKPAVAQLFRAQQIAESQPA
>Mature_504_residues
MNLPLVHRAYEHGPRTAIVTADGQFSYQDLLSLSSQVATRLLDGADDLREQRVASLVPAGFEYVATQWGIWRAGGIAVPL
CVSHPRPELEYVITNSGASIVIAHPSFESLLRPIAEAHQLRFILTSESLPPDTAQLPEVEPTRRALILYTSGTTGKPKGV
VTTHGNIQAQVTSLISAWEWTSGDRILHVLPLHHIHGIVNVLTCALWAGAECHMLSKFDAETVWEHICEGNFTLFMAVPT
IYTKLIATWEKAAGDRQMDMSRGCAGMRLMVSGSAALPVQVLERWTHISGHFLLERYGMTEIGMALSNPLHGQRRAGYVG
MALPQVDVRLVDESGVSVPAGTPGEIQVKGPGVFLEYWQKPEATAKAFQDGWFCTGDLAVVEQDCYRILGRISVDIIKTG
GYKVSALEIEEVLRTHPDIQECAVVGVADPEWGERVCGALVLQSGCNLALEPFRSWAKERLAAYKVPTRILSVEELPRNA
MGKVTKPAVAQLFRAQQIAESQPA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=529, Percent_Identity=45.9357277882798, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI187761343, Length=529, Percent_Identity=45.9357277882798, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI156151445, Length=479, Percent_Identity=28.6012526096033, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI38505220, Length=500, Percent_Identity=27.4, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI58082049, Length=490, Percent_Identity=27.1428571428571, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI157311624, Length=490, Percent_Identity=26.734693877551, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI157311622, Length=490, Percent_Identity=26.734693877551, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI42544132, Length=494, Percent_Identity=23.4817813765182, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI122937307, Length=500, Percent_Identity=25.6, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI115511026, Length=525, Percent_Identity=24.7619047619048, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI13375727, Length=381, Percent_Identity=23.8845144356955, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI38524616, Length=418, Percent_Identity=25.3588516746411, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI40807357, Length=533, Percent_Identity=24.015009380863, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI28416953, Length=387, Percent_Identity=26.3565891472868, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI13325057, Length=449, Percent_Identity=25.1670378619154, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788107, Length=523, Percent_Identity=29.2543021032505, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI145693145, Length=467, Percent_Identity=29.9785867237687, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI221142682, Length=454, Percent_Identity=30.6167400881057, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1786810, Length=526, Percent_Identity=25.8555133079848, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1789201, Length=370, Percent_Identity=29.4594594594595, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788595, Length=361, Percent_Identity=24.0997229916898, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786801, Length=502, Percent_Identity=23.9043824701195, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790505, Length=380, Percent_Identity=25, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17558820, Length=486, Percent_Identity=32.7160493827161, Blast_Score=250, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI32563687, Length=490, Percent_Identity=31.2244897959184, Blast_Score=244, Evalue=8e-65,
Organism=Caenorhabditis elegans, GI17559526, Length=360, Percent_Identity=28.3333333333333, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI71994703, Length=535, Percent_Identity=26.1682242990654, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71994694, Length=535, Percent_Identity=26.1682242990654, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71994690, Length=535, Percent_Identity=26.1682242990654, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17531443, Length=463, Percent_Identity=24.622030237581, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=531, Percent_Identity=26.5536723163842, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17560140, Length=366, Percent_Identity=27.0491803278689, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=532, Percent_Identity=24.812030075188, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI71996755, Length=516, Percent_Identity=24.4186046511628, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=405, Percent_Identity=28.641975308642, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17538037, Length=518, Percent_Identity=24.3243243243243, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17510401, Length=275, Percent_Identity=28.3636363636364, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=529, Percent_Identity=25.8979206049149, Blast_Score=180, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6323903, Length=416, Percent_Identity=26.4423076923077, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320852, Length=355, Percent_Identity=27.6056338028169, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6319264, Length=370, Percent_Identity=24.5945945945946, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI221329755, Length=560, Percent_Identity=25.3571428571429, Blast_Score=178, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24581924, Length=557, Percent_Identity=27.1095152603232, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI18859661, Length=514, Percent_Identity=24.9027237354086, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI21355181, Length=455, Percent_Identity=24.3956043956044, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21356947, Length=374, Percent_Identity=26.7379679144385, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648257, Length=362, Percent_Identity=25.414364640884, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI20130357, Length=369, Percent_Identity=23.8482384823848, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281365686, Length=370, Percent_Identity=25.4054054054054, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648260, Length=379, Percent_Identity=25.3298153034301, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI17933690, Length=340, Percent_Identity=25.2941176470588, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356441, Length=351, Percent_Identity=25.0712250712251, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922652, Length=371, Percent_Identity=23.4501347708895, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24667959, Length=371, Percent_Identity=24.7978436657682, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24667955, Length=368, Percent_Identity=25, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI62472339, Length=371, Percent_Identity=24.7978436657682, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648253, Length=445, Percent_Identity=23.3707865168539, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24653035, Length=233, Percent_Identity=27.8969957081545, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648255, Length=445, Percent_Identity=23.3707865168539, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21358303, Length=329, Percent_Identity=21.580547112462, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583465, Length=524, Percent_Identity=22.5190839694656, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24583463, Length=524, Percent_Identity=22.5190839694656, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI281364798, Length=524, Percent_Identity=22.5190839694656, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI161076582, Length=237, Percent_Identity=24.0506329113924, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 55182; Mature: 55182

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPLVHRAYEHGPRTAIVTADGQFSYQDLLSLSSQVATRLLDGADDLREQRVASLVPAG
CCCCCCHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
FEYVATQWGIWRAGGIAVPLCVSHPRPELEYVITNSGASIVIAHPSFESLLRPIAEAHQL
HHHHHHCCCEEECCCEEEEEEECCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHE
RFILTSESLPPDTAQLPEVEPTRRALILYTSGTTGKPKGVVTTHGNIQAQVTSLISAWEW
EEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCC
TSGDRILHVLPLHHIHGIVNVLTCALWAGAECHMLSKFDAETVWEHICEGNFTLFMAVPT
CCCCCEEEEECHHHHHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHHHHCCCCEEEEEHHH
IYTKLIATWEKAAGDRQMDMSRGCAGMRLMVSGSAALPVQVLERWTHISGHFLLERYGMT
HHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHCCCH
EIGMALSNPLHGQRRAGYVGMALPQVDVRLVDESGVSVPAGTPGEIQVKGPGVFLEYWQK
HHHHHHCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCEEEEECCCEEEEECCC
PEATAKAFQDGWFCTGDLAVVEQDCYRILGRISVDIIKTGGYKVSALEIEEVLRTHPDIQ
CCHHHHHHHCCCEEECCHHHHHHHHHHHHHHCEEEEEECCCEEEEHEEHHHHHHCCCCHH
ECAVVGVADPEWGERVCGALVLQSGCNLALEPFRSWAKERLAAYKVPTRILSVEELPRNA
HEEEEECCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHH
MGKVTKPAVAQLFRAQQIAESQPA
HCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLPLVHRAYEHGPRTAIVTADGQFSYQDLLSLSSQVATRLLDGADDLREQRVASLVPAG
CCCCCCHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
FEYVATQWGIWRAGGIAVPLCVSHPRPELEYVITNSGASIVIAHPSFESLLRPIAEAHQL
HHHHHHCCCEEECCCEEEEEEECCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHE
RFILTSESLPPDTAQLPEVEPTRRALILYTSGTTGKPKGVVTTHGNIQAQVTSLISAWEW
EEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCC
TSGDRILHVLPLHHIHGIVNVLTCALWAGAECHMLSKFDAETVWEHICEGNFTLFMAVPT
CCCCCEEEEECHHHHHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHHHHCCCCEEEEEHHH
IYTKLIATWEKAAGDRQMDMSRGCAGMRLMVSGSAALPVQVLERWTHISGHFLLERYGMT
HHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHCCCH
EIGMALSNPLHGQRRAGYVGMALPQVDVRLVDESGVSVPAGTPGEIQVKGPGVFLEYWQK
HHHHHHCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCEEEEECCCEEEEECCC
PEATAKAFQDGWFCTGDLAVVEQDCYRILGRISVDIIKTGGYKVSALEIEEVLRTHPDIQ
CCHHHHHHHCCCEEECCHHHHHHHHHHHHHHCEEEEEECCCEEEEHEEHHHHHHCCCCHH
ECAVVGVADPEWGERVCGALVLQSGCNLALEPFRSWAKERLAAYKVPTRILSVEELPRNA
HEEEEECCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHH
MGKVTKPAVAQLFRAQQIAESQPA
HCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]