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The map label for this gene is thiC

Identifier: 37520687

GI number: 37520687

Start: 1196979

End: 1198358

Strand: Direct

Name: thiC

Synonym: gvip148

Alternate gene names: 37520687

Gene position: 1196979-1198358 (Clockwise)

Preceding gene: 37520685

Following gene: 37520690

Centisome position: 25.69

GC content: 63.84

Gene sequence:

>1380_bases
ATGCTACGCACCGAATGGATCGCGCGCCGGCACGGGCACGCCAATGTCTCGCAGATGTACTACGCCCGCCAGGGGTTGAT
CACCGAGGAGGTGGCCTACGTCGCCCACCGCGAAAACCTGGAGCCCGAACTCGTCCGCTCCGAGGTGGCCCGCGGCCGGC
TGATCATCCCCGCCAACACCAATCACCCCAACCTGGAGCCGATGGGCATCGGCATCGCCACCCGCTGCAAAGTCAACGCC
AACATCGGCGCCTCCCCCAACGCCTCGAACCTGGGCGAGGAAGTGGCCAAACTGGAACTGGCCGTCAAGTACGGCGCCGA
CACGGTGATGGATCTATCCACCGGCGGCGGCGATCTGGACGCCATCCGCAGTGCCATCATCCAGGCAAGTCCGGTGCCCA
TCGGCACGGTGCCGATGTACCAGGCCCTCGAAGCGGTCCACGGCAACGTCGAGAAGCTCACCCCCGAACACATCCTCGAT
GTAATCGAAAAGCACGCGAAGCAGGGGGTCGATTACATGACCATCCACGCCGGGATCTTAATTGAGCACCTGCCTTTGAC
CCAGGGTCGCCTCACCGGCATCGTCTCGCGCGGCGGCGGCATCCTGGCGCGCTGGATGCTTGCCCACCACAAACAAAACC
CGCTCTACACGCACTTCGACGAGATTATCAACATCTTCAAGAAGTACGACGTCTCCTTCAGCCTGGGCGATTCGCTGAGG
CCCGGCTGCCAGCACGACGCGAGCGACGCCGCCCAGTTCGCCGAATTGAAGACCCTAGGCGAACTCACCCGCCGCGCCTG
GACCCACGATGTGCAGGTGATGGTCGAAGGCCCCGGCCACGTGCCGATGCACCAGATCGAGATGAACGTGCGCAAGCAGA
TGCAGGAATGCTCCGAAGCGCCCTTTTATGTCCTGGGTCCCCTGGTCACCGACATCGCCCCGGGCTATGACCACATCACC
AGCGCCATCGGCGCTGCCCTGGCCGGCTGGTACGGCACCGCCATGCTCTGCTATGTGACCCCCAAGGAGCACCTGGGCCT
GCCCAACGCCGAGGATGTGCGCACAGGGCTCATCGCCTACAAAATTGCCGCCCACGCCGCCGACATCGCCCGCGGCCGTC
CGGGGGCGCGCGATCGCGACGACGAACTATCACGCGCGCGCTACAACTTCGACTGGAACCGCCAGTTCGAACTTTCGCTC
GACCCGGAGCGCGCCCGCGAATACCACGACGAGACGTTGCCGGCAGATATCTACAAATCCGCCGAATTCTGCTCGATGTG
CGGCCCCAAGTTCTGCCCGATGCAAACCAAAATCGACTCGCAAGCCTTGAGTGAACTGGAAGCGGCCCTCAAGCCCCAAC
CGGTCGCTGCTCAGGAGTAG

Upstream 100 bases:

>100_bases
CGCTGGGGGTGCGGACGGTCCGCACGATCGCTTCGCTGAGATCACACCCCTTGAACCTGATCCGGTTCGTACCGGCGAGA
GGGAAGCCAAAGGAGTCACT

Downstream 100 bases:

>100_bases
GACTTCGGATTTATTTTGAAAGGGATGTCATCAAGGCATCCCTTTCGCTTAAGTAAAACGCACTAACTGAAAAGATCCTC
CTCAGACAGCCCTGGGCTTC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLRTEWIARRHGHANVSQMYYARQGLITEEVAYVAHRENLEPELVRSEVARGRLIIPANTNHPNLEPMGIGIATRCKVNA
NIGASPNASNLGEEVAKLELAVKYGADTVMDLSTGGGDLDAIRSAIIQASPVPIGTVPMYQALEAVHGNVEKLTPEHILD
VIEKHAKQGVDYMTIHAGILIEHLPLTQGRLTGIVSRGGGILARWMLAHHKQNPLYTHFDEIINIFKKYDVSFSLGDSLR
PGCQHDASDAAQFAELKTLGELTRRAWTHDVQVMVEGPGHVPMHQIEMNVRKQMQECSEAPFYVLGPLVTDIAPGYDHIT
SAIGAALAGWYGTAMLCYVTPKEHLGLPNAEDVRTGLIAYKIAAHAADIARGRPGARDRDDELSRARYNFDWNRQFELSL
DPERAREYHDETLPADIYKSAEFCSMCGPKFCPMQTKIDSQALSELEAALKPQPVAAQE

Sequences:

>Translated_459_residues
MLRTEWIARRHGHANVSQMYYARQGLITEEVAYVAHRENLEPELVRSEVARGRLIIPANTNHPNLEPMGIGIATRCKVNA
NIGASPNASNLGEEVAKLELAVKYGADTVMDLSTGGGDLDAIRSAIIQASPVPIGTVPMYQALEAVHGNVEKLTPEHILD
VIEKHAKQGVDYMTIHAGILIEHLPLTQGRLTGIVSRGGGILARWMLAHHKQNPLYTHFDEIINIFKKYDVSFSLGDSLR
PGCQHDASDAAQFAELKTLGELTRRAWTHDVQVMVEGPGHVPMHQIEMNVRKQMQECSEAPFYVLGPLVTDIAPGYDHIT
SAIGAALAGWYGTAMLCYVTPKEHLGLPNAEDVRTGLIAYKIAAHAADIARGRPGARDRDDELSRARYNFDWNRQFELSL
DPERAREYHDETLPADIYKSAEFCSMCGPKFCPMQTKIDSQALSELEAALKPQPVAAQE
>Mature_459_residues
MLRTEWIARRHGHANVSQMYYARQGLITEEVAYVAHRENLEPELVRSEVARGRLIIPANTNHPNLEPMGIGIATRCKVNA
NIGASPNASNLGEEVAKLELAVKYGADTVMDLSTGGGDLDAIRSAIIQASPVPIGTVPMYQALEAVHGNVEKLTPEHILD
VIEKHAKQGVDYMTIHAGILIEHLPLTQGRLTGIVSRGGGILARWMLAHHKQNPLYTHFDEIINIFKKYDVSFSLGDSLR
PGCQHDASDAAQFAELKTLGELTRRAWTHDVQVMVEGPGHVPMHQIEMNVRKQMQECSEAPFYVLGPLVTDIAPGYDHIT
SAIGAALAGWYGTAMLCYVTPKEHLGLPNAEDVRTGLIAYKIAAHAADIARGRPGARDRDDELSRARYNFDWNRQFELSL
DPERAREYHDETLPADIYKSAEFCSMCGPKFCPMQTKIDSQALSELEAALKPQPVAAQE

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=447, Percent_Identity=59.0604026845638, Blast_Score=535, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_GLOVI (Q7NLK5)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_924064.1
- GeneID:   2598985
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.1118
- HOGENOM:   HBG285234
- OMA:   EPMAIGI
- ProtClustDB:   PRK13352
- BioCyc:   GVIO251221:GLR1118-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 50648; Mature: 50648

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: BINDING 81-81 BINDING 110-110 BINDING 140-140 BINDING 176-176 BINDING 276-276 BINDING 303-303

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRTEWIARRHGHANVSQMYYARQGLITEEVAYVAHRENLEPELVRSEVARGRLIIPANT
CCCCHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCC
NHPNLEPMGIGIATRCKVNANIGASPNASNLGEEVAKLELAVKYGADTVMDLSTGGGDLD
CCCCCCEECCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
AIRSAIIQASPVPIGTVPMYQALEAVHGNVEKLTPEHILDVIEKHAKQGVDYMTIHAGIL
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHCCCCEEEEEHHHH
IEHLPLTQGRLTGIVSRGGGILARWMLAHHKQNPLYTHFDEIINIFKKYDVSFSLGDSLR
HCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEECCCCCC
PGCQHDASDAAQFAELKTLGELTRRAWTHDVQVMVEGPGHVPMHQIEMNVRKQMQECSEA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCC
PFYVLGPLVTDIAPGYDHITSAIGAALAGWYGTAMLCYVTPKEHLGLPNAEDVRTGLIAY
CEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHHHHHHH
KIAAHAADIARGRPGARDRDDELSRARYNFDWNRQFELSLDPERAREYHDETLPADIYKS
HHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCCCHHHHHH
AEFCSMCGPKFCPMQTKIDSQALSELEAALKPQPVAAQE
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLRTEWIARRHGHANVSQMYYARQGLITEEVAYVAHRENLEPELVRSEVARGRLIIPANT
CCCCHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCC
NHPNLEPMGIGIATRCKVNANIGASPNASNLGEEVAKLELAVKYGADTVMDLSTGGGDLD
CCCCCCEECCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
AIRSAIIQASPVPIGTVPMYQALEAVHGNVEKLTPEHILDVIEKHAKQGVDYMTIHAGIL
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHCCCCEEEEEHHHH
IEHLPLTQGRLTGIVSRGGGILARWMLAHHKQNPLYTHFDEIINIFKKYDVSFSLGDSLR
HCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEECCCCCC
PGCQHDASDAAQFAELKTLGELTRRAWTHDVQVMVEGPGHVPMHQIEMNVRKQMQECSEA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCC
PFYVLGPLVTDIAPGYDHITSAIGAALAGWYGTAMLCYVTPKEHLGLPNAEDVRTGLIAY
CEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHHHHHHH
KIAAHAADIARGRPGARDRDDELSRARYNFDWNRQFELSLDPERAREYHDETLPADIYKS
HHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCCCHHHHHH
AEFCSMCGPKFCPMQTKIDSQALSELEAALKPQPVAAQE
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292