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The map label for this gene is folC [H]

Identifier: 37520643

GI number: 37520643

Start: 1150393

End: 1151634

Strand: Direct

Name: folC [H]

Synonym: gvip147

Alternate gene names: 37520643

Gene position: 1150393-1151634 (Clockwise)

Preceding gene: 37520642

Following gene: 37520644

Centisome position: 24.69

GC content: 62.56

Gene sequence:

>1242_bases
ATGACGGCTGGAGGTTATCTCGATCACCTGCAAAAATTTGGCGTTCACCTGGGCTTGGAGCGCATCCAGGCGCTGCTTAA
GCGCCTGGGGGAGCCGCAGGTTGCCTTCCAAGCCGTGCATGTGGCGGGGACCAATGGTAAAGGCTCGGTCTGCGCTTATC
TGAGCCATATTTTGCAGACGGCCGGGTATCGGACGGGGCGCTACACTTCACCCCATTTGCTCGACTGGAACGAGCGCATC
TGGATTGACGGTGCTTTTATCTCGGATGGGGCTTTGCAGGCGTGCCTGGCAAAAGTGAAAGCCTCCGCCGAGCATTTGCC
GAAAAGTTTGGGGGAGCCTACCCAGTTCGAGATTGTCACGGCCGCTGCGTTCTTGCACTTTGCCCAATCTGCCGTCGAAG
TGGCGGTAGTCGAGGTCGGACTGGGCGGACGACTCGACGCCACCAACGTCTTCGAGCAGCCGCCGGCGAGCGTGATCACC
GGTATCGGCCTGGATCACTGCGACCAACTCGGCTCCACGCTGGCGGCCATCGCCGCCGAAAAAGCCGGGATTTTGCGGTC
CGGCTGCCCCCTGGTCTGCGCGCCGCTGGCCCCGGAAGCGATGCAGGTGGTCACTGAAAAGGCACTGGCGTTGGGCGTTG
TGATCACCTTGGCCGAGCCTGCCCACGAGGTCGCCCCGGGTGAGGCGCAGTGGCAAGGATTTCGTTACCGGCTCCCCCTC
GCAGGTGGGGTACAGCTGCAAAACAGCGCCACCGCCCTCGCCACCTGCCAAGTGCTCCGGGAGCGGGGACTGCCCATTGA
TGAAACAGCGATCCGCACCGGCCTCGAAAACACCGTTTGGCCGGGACGCTACCAGTGGCTTGGCGAACGGTTGCTGCTCG
ACGGTGCCCACAACGAAGACAGTGCCGTGTACCTGCGCCGCTATCTGGATACCCTCCACCCCAAAGAATCCATCCGCTGG
ATCGTGGGCATTCTCGAAAGCAAGGACGCGCGAGCCGTCTTAAAAGCTTTGCTCCGTCCGGGCGACGCTTTTGTAGCCGT
CCCCGTTCCCGACGCCCGCTCCCATCCGCCCGAGAGGCTGGCCTCCATGGCGCAGACCATGGGCATACACTCCATCGCAA
GCGCCTCCGGTTTTTCTGAAGCCCTTCAGCTTAGCGATACAGAGCAGTTGACGGTGATCGCCGGCTCTTTGTACCTGGCG
GGGGCGGTGCTGCGGGACTGGCCGCGCCTCCAAAAGCAATGA

Upstream 100 bases:

>100_bases
CGTGTCGACTGCGGCGATGGGGCTGGCTCGCCACTGAGCTGCAGGAGTGTCCAGTGCGTCACAATAGTCCAAATAATGTA
GATATCTACTACCCAAACCA

Downstream 100 bases:

>100_bases
AGCGATTCGTGGCCGCTGCGGCTGGAAGACCTTCGGGACCAACCGATAGCATGGGAAAGATTTTCTCAGGTGCTCCAGGA
TGCTCACCGCCGATCCGGCC

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MTAGGYLDHLQKFGVHLGLERIQALLKRLGEPQVAFQAVHVAGTNGKGSVCAYLSHILQTAGYRTGRYTSPHLLDWNERI
WIDGAFISDGALQACLAKVKASAEHLPKSLGEPTQFEIVTAAAFLHFAQSAVEVAVVEVGLGGRLDATNVFEQPPASVIT
GIGLDHCDQLGSTLAAIAAEKAGILRSGCPLVCAPLAPEAMQVVTEKALALGVVITLAEPAHEVAPGEAQWQGFRYRLPL
AGGVQLQNSATALATCQVLRERGLPIDETAIRTGLENTVWPGRYQWLGERLLLDGAHNEDSAVYLRRYLDTLHPKESIRW
IVGILESKDARAVLKALLRPGDAFVAVPVPDARSHPPERLASMAQTMGIHSIASASGFSEALQLSDTEQLTVIAGSLYLA
GAVLRDWPRLQKQ

Sequences:

>Translated_413_residues
MTAGGYLDHLQKFGVHLGLERIQALLKRLGEPQVAFQAVHVAGTNGKGSVCAYLSHILQTAGYRTGRYTSPHLLDWNERI
WIDGAFISDGALQACLAKVKASAEHLPKSLGEPTQFEIVTAAAFLHFAQSAVEVAVVEVGLGGRLDATNVFEQPPASVIT
GIGLDHCDQLGSTLAAIAAEKAGILRSGCPLVCAPLAPEAMQVVTEKALALGVVITLAEPAHEVAPGEAQWQGFRYRLPL
AGGVQLQNSATALATCQVLRERGLPIDETAIRTGLENTVWPGRYQWLGERLLLDGAHNEDSAVYLRRYLDTLHPKESIRW
IVGILESKDARAVLKALLRPGDAFVAVPVPDARSHPPERLASMAQTMGIHSIASASGFSEALQLSDTEQLTVIAGSLYLA
GAVLRDWPRLQKQ
>Mature_412_residues
TAGGYLDHLQKFGVHLGLERIQALLKRLGEPQVAFQAVHVAGTNGKGSVCAYLSHILQTAGYRTGRYTSPHLLDWNERIW
IDGAFISDGALQACLAKVKASAEHLPKSLGEPTQFEIVTAAAFLHFAQSAVEVAVVEVGLGGRLDATNVFEQPPASVITG
IGLDHCDQLGSTLAAIAAEKAGILRSGCPLVCAPLAPEAMQVVTEKALALGVVITLAEPAHEVAPGEAQWQGFRYRLPLA
GGVQLQNSATALATCQVLRERGLPIDETAIRTGLENTVWPGRYQWLGERLLLDGAHNEDSAVYLRRYLDTLHPKESIRWI
VGILESKDARAVLKALLRPGDAFVAVPVPDARSHPPERLASMAQTMGIHSIASASGFSEALQLSDTEQLTVIAGSLYLAG
AVLRDWPRLQKQ

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=392, Percent_Identity=31.8877551020408, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI66932990, Length=392, Percent_Identity=31.8877551020408, Blast_Score=135, Evalue=6e-32,
Organism=Escherichia coli, GI1788654, Length=407, Percent_Identity=34.1523341523341, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI71984923, Length=293, Percent_Identity=32.7645051194539, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17553148, Length=293, Percent_Identity=32.7645051194539, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17553150, Length=293, Percent_Identity=32.7645051194539, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323760, Length=423, Percent_Identity=33.806146572104, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6324815, Length=343, Percent_Identity=31.1953352769679, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322718, Length=422, Percent_Identity=23.9336492890995, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24641571, Length=353, Percent_Identity=33.1444759206799, Blast_Score=151, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24581568, Length=333, Percent_Identity=27.6276276276276, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 44282; Mature: 44151

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGGYLDHLQKFGVHLGLERIQALLKRLGEPQVAFQAVHVAGTNGKGSVCAYLSHILQT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
AGYRTGRYTSPHLLDWNERIWIDGAFISDGALQACLAKVKASAEHLPKSLGEPTQFEIVT
CCCCCCCCCCCCEECCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AAAFLHFAQSAVEVAVVEVGLGGRLDATNVFEQPPASVITGIGLDHCDQLGSTLAAIAAE
HHHHHHHHHHHHEEEEEEECCCCCCCCCCHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHH
KAGILRSGCPLVCAPLAPEAMQVVTEKALALGVVITLAEPAHEVAPGEAQWQGFRYRLPL
HHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHEEECCCHHHCCCCCHHCCCEEEEECC
AGGVQLQNSATALATCQVLRERGLPIDETAIRTGLENTVWPGRYQWLGERLLLDGAHNED
CCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHEECCCCCCC
SAVYLRRYLDTLHPKESIRWIVGILESKDARAVLKALLRPGDAFVAVPVPDARSHPPERL
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHH
ASMAQTMGIHSIASASGFSEALQLSDTEQLTVIAGSLYLAGAVLRDWPRLQKQ
HHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAGGYLDHLQKFGVHLGLERIQALLKRLGEPQVAFQAVHVAGTNGKGSVCAYLSHILQT
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
AGYRTGRYTSPHLLDWNERIWIDGAFISDGALQACLAKVKASAEHLPKSLGEPTQFEIVT
CCCCCCCCCCCCEECCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AAAFLHFAQSAVEVAVVEVGLGGRLDATNVFEQPPASVITGIGLDHCDQLGSTLAAIAAE
HHHHHHHHHHHHEEEEEEECCCCCCCCCCHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHH
KAGILRSGCPLVCAPLAPEAMQVVTEKALALGVVITLAEPAHEVAPGEAQWQGFRYRLPL
HHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHEEECCCHHHCCCCCHHCCCEEEEECC
AGGVQLQNSATALATCQVLRERGLPIDETAIRTGLENTVWPGRYQWLGERLLLDGAHNED
CCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHEECCCCCCC
SAVYLRRYLDTLHPKESIRWIVGILESKDARAVLKALLRPGDAFVAVPVPDARSHPPERL
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHH
ASMAQTMGIHSIASASGFSEALQLSDTEQLTVIAGSLYLAGAVLRDWPRLQKQ
HHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]